Antioxidant effect of Morus nigra on Chagas disease progression
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/140687 |
Resumo: | Considering the widespread popular use of Morus nigra and the amount of scientific information on its antioxidant and anti-inflammatory activity, the effectiveness of this phytotherapeutic compound in the parasitemia progression during the acute phase of Chagas disease and its role in the development of the inflammatory process as well as its effects on the oxidative damage in the chronic phase of infection were evaluated. Thus, 96 male Swiss mice were randomly divided into eight groups, four groups were uninfected controls, and four groups were intraperitoneally infected with 5.0 x 104 blood trypomastigotes forms of T. cruzi QM2 strain. Four batches composed of one uninfected and one infected group were respectively treated with 70% alcohol solution and 25 μL, 50 μL and 75 μL of the phytotherapeutic compound. Levels of antioxidant elements (TBARS, FRAP, GSH and Sulfhydryl groups) were measured in plasma samples. The phytotherapeutic compound’s antioxidant activity was measured by polyphenol and total flavonoid quantification, DPPH, NO, and FRAP method. Our results showed that the vehicle influenced some of the results that may have physiological relevance in Chagas disease. However, an important action of M. nigra tincture was observed in the progression of Chagas disease, since our results demonstrated a reduction in parasitemia of treated groups when compared to controls, especially in the group receiving 25 µL. However, in the chronic phase, the 50-µL dosage presented a better activity on some antioxidant defenses and minimized the tissue inflammatory process. Results indicated an important action of M. nigra tincture on the Chagas disease progression. |
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Antioxidant effect of Morus nigra on Chagas disease progressionPhytotherapyHerbal medicineHerbal compoundsPhytotherapeutic compoundPlant extractsParasitic diseasesBlackberryOxidative stress Considering the widespread popular use of Morus nigra and the amount of scientific information on its antioxidant and anti-inflammatory activity, the effectiveness of this phytotherapeutic compound in the parasitemia progression during the acute phase of Chagas disease and its role in the development of the inflammatory process as well as its effects on the oxidative damage in the chronic phase of infection were evaluated. Thus, 96 male Swiss mice were randomly divided into eight groups, four groups were uninfected controls, and four groups were intraperitoneally infected with 5.0 x 104 blood trypomastigotes forms of T. cruzi QM2 strain. Four batches composed of one uninfected and one infected group were respectively treated with 70% alcohol solution and 25 μL, 50 μL and 75 μL of the phytotherapeutic compound. Levels of antioxidant elements (TBARS, FRAP, GSH and Sulfhydryl groups) were measured in plasma samples. The phytotherapeutic compound’s antioxidant activity was measured by polyphenol and total flavonoid quantification, DPPH, NO, and FRAP method. Our results showed that the vehicle influenced some of the results that may have physiological relevance in Chagas disease. However, an important action of M. nigra tincture was observed in the progression of Chagas disease, since our results demonstrated a reduction in parasitemia of treated groups when compared to controls, especially in the group receiving 25 µL. However, in the chronic phase, the 50-µL dosage presented a better activity on some antioxidant defenses and minimized the tissue inflammatory process. Results indicated an important action of M. nigra tincture on the Chagas disease progression.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2017-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/xmlhttps://www.revistas.usp.br/rimtsp/article/view/140687Revista do Instituto de Medicina Tropical de São Paulo; Vol. 59 (2017); e73Revista do Instituto de Medicina Tropical de São Paulo; Vol. 59 (2017); e73Revista do Instituto de Medicina Tropical de São Paulo; v. 59 (2017); e731678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/140687/135650https://www.revistas.usp.br/rimtsp/article/view/140687/148459Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessMontenote, Michelly CristinaWajsman, Vithor ZuccaroKonno, Yoichi TakakiFerreira, Paulo CésarSilva, Regildo Márcio GonçalvesTherezo, Altino Luiz SilvaSilva, Luciana PereiraMartins, Luciamáre Perinetti Alves2018-02-23T18:46:02Zoai:revistas.usp.br:article/140687Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:41.453683Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Antioxidant effect of Morus nigra on Chagas disease progression |
title |
Antioxidant effect of Morus nigra on Chagas disease progression |
spellingShingle |
Antioxidant effect of Morus nigra on Chagas disease progression Montenote, Michelly Cristina Phytotherapy Herbal medicine Herbal compounds Phytotherapeutic compound Plant extracts Parasitic diseases Blackberry Oxidative stress |
title_short |
Antioxidant effect of Morus nigra on Chagas disease progression |
title_full |
Antioxidant effect of Morus nigra on Chagas disease progression |
title_fullStr |
Antioxidant effect of Morus nigra on Chagas disease progression |
title_full_unstemmed |
Antioxidant effect of Morus nigra on Chagas disease progression |
title_sort |
Antioxidant effect of Morus nigra on Chagas disease progression |
author |
Montenote, Michelly Cristina |
author_facet |
Montenote, Michelly Cristina Wajsman, Vithor Zuccaro Konno, Yoichi Takaki Ferreira, Paulo César Silva, Regildo Márcio Gonçalves Therezo, Altino Luiz Silva Silva, Luciana Pereira Martins, Luciamáre Perinetti Alves |
author_role |
author |
author2 |
Wajsman, Vithor Zuccaro Konno, Yoichi Takaki Ferreira, Paulo César Silva, Regildo Márcio Gonçalves Therezo, Altino Luiz Silva Silva, Luciana Pereira Martins, Luciamáre Perinetti Alves |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Montenote, Michelly Cristina Wajsman, Vithor Zuccaro Konno, Yoichi Takaki Ferreira, Paulo César Silva, Regildo Márcio Gonçalves Therezo, Altino Luiz Silva Silva, Luciana Pereira Martins, Luciamáre Perinetti Alves |
dc.subject.por.fl_str_mv |
Phytotherapy Herbal medicine Herbal compounds Phytotherapeutic compound Plant extracts Parasitic diseases Blackberry Oxidative stress |
topic |
Phytotherapy Herbal medicine Herbal compounds Phytotherapeutic compound Plant extracts Parasitic diseases Blackberry Oxidative stress |
description |
Considering the widespread popular use of Morus nigra and the amount of scientific information on its antioxidant and anti-inflammatory activity, the effectiveness of this phytotherapeutic compound in the parasitemia progression during the acute phase of Chagas disease and its role in the development of the inflammatory process as well as its effects on the oxidative damage in the chronic phase of infection were evaluated. Thus, 96 male Swiss mice were randomly divided into eight groups, four groups were uninfected controls, and four groups were intraperitoneally infected with 5.0 x 104 blood trypomastigotes forms of T. cruzi QM2 strain. Four batches composed of one uninfected and one infected group were respectively treated with 70% alcohol solution and 25 μL, 50 μL and 75 μL of the phytotherapeutic compound. Levels of antioxidant elements (TBARS, FRAP, GSH and Sulfhydryl groups) were measured in plasma samples. The phytotherapeutic compound’s antioxidant activity was measured by polyphenol and total flavonoid quantification, DPPH, NO, and FRAP method. Our results showed that the vehicle influenced some of the results that may have physiological relevance in Chagas disease. However, an important action of M. nigra tincture was observed in the progression of Chagas disease, since our results demonstrated a reduction in parasitemia of treated groups when compared to controls, especially in the group receiving 25 µL. However, in the chronic phase, the 50-µL dosage presented a better activity on some antioxidant defenses and minimized the tissue inflammatory process. Results indicated an important action of M. nigra tincture on the Chagas disease progression. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/140687 |
url |
https://www.revistas.usp.br/rimtsp/article/view/140687 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/140687/135650 https://www.revistas.usp.br/rimtsp/article/view/140687/148459 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/xml |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 59 (2017); e73 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 59 (2017); e73 Revista do Instituto de Medicina Tropical de São Paulo; v. 59 (2017); e73 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951651743105024 |