Preliminary investigation of Culicidae species in South Pantanal, Brazil and their potential importance in arbovirus transmission
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/31296 |
Resumo: | In view of the high circulation of migratory birds and the environmental and climatic conditions which favor the proliferation of arthropods, the Brazilian Pantanal is susceptible to circulation of arboviruses. However, the amount of data concerning arbovirus vectors in this area is scarce; therefore the aim of this study was to conduct a preliminary investigation of Culicidae species in the Nhecolândia Sub-region of South Pantanal, Brazil and their potential importance in the arbovirus transmission. A total of 3684 specimens of mosquitoes were captured, 1689 of which caught in the rainy season of 2007, were divided into 78 pools and submitted to viral isolation, Semi-Nested RT-PCR and Nested RT-PCR, with a view to identifying the most important arboviruses in Brazil. Simultaneously, 70 specimens of ticks found blood-feeding on horses were also submitted to the same virological assays. No virus was isolated and viral nucleic-acid detection by RT-PCR was also negative. Nevertheless, a total of 22 Culicidae species were identified, ten of which had previously been reported as vectors of important arboviruses. The diversity of species found blood-feeding on human and horse hosts together with the arboviruses circulation previously reported suggest that the Nhecolândia Sub-region of South Pantanal is an important area for arbovirus surveillance in Brazil. |
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Preliminary investigation of Culicidae species in South Pantanal, Brazil and their potential importance in arbovirus transmission Investigação preliminar das espécies de culicídeos do Pantanal Sul brasileiro e sua potencial importância na transmissão de arbovírus ArbovirusPantanalCulicidaeTicksNhecolândia In view of the high circulation of migratory birds and the environmental and climatic conditions which favor the proliferation of arthropods, the Brazilian Pantanal is susceptible to circulation of arboviruses. However, the amount of data concerning arbovirus vectors in this area is scarce; therefore the aim of this study was to conduct a preliminary investigation of Culicidae species in the Nhecolândia Sub-region of South Pantanal, Brazil and their potential importance in the arbovirus transmission. A total of 3684 specimens of mosquitoes were captured, 1689 of which caught in the rainy season of 2007, were divided into 78 pools and submitted to viral isolation, Semi-Nested RT-PCR and Nested RT-PCR, with a view to identifying the most important arboviruses in Brazil. Simultaneously, 70 specimens of ticks found blood-feeding on horses were also submitted to the same virological assays. No virus was isolated and viral nucleic-acid detection by RT-PCR was also negative. Nevertheless, a total of 22 Culicidae species were identified, ten of which had previously been reported as vectors of important arboviruses. The diversity of species found blood-feeding on human and horse hosts together with the arboviruses circulation previously reported suggest that the Nhecolândia Sub-region of South Pantanal is an important area for arbovirus surveillance in Brazil. Regiões como o Pantanal brasileiro, que apresentam fatores como riqueza de fauna silvestre incluindo circulação de aves migratórias e condições ambientais e climáticas favoráveis à proliferação de artrópodes estão potencialmente sujeitas à circulação de arbovírus. Entretanto, poucos trabalhos foram realizados acerca da presença de arbovírus em potenciais vetores no Pantanal. Neste sentido o principal objetivo deste trabalho foi conduzir uma investigação preliminar para presença de arbovírus em amostragens de culicídeos capturados na Sub-região da Nhecolândia no Pantanal Sul. Um total de 3684 mosquitos foi capturado, dos quais 78 grupos compondo uma amostragem de 1789 espécimes foram submetidos às técnicas de isolamento viral e RT-PCR para os mais importantes arbovírus no Brasil. Simultaneamente, 70 espécimes de carrapatos capturados durante hematofagia em cavalos também foram submetidos à pesquisa viral. Não houve isolamento viral em nenhuma amostra analisada e os resultados de detecção de ácido nucléico viral foram também negativos. Entretanto, foram identificadas 22 espécies de culicídeos, dez das quais previamente reportadas como vetores de importantes arbovírus. A competência vetorial de espécies capturadas durante hematofagia em humanos e cavalos aliada ao relato prévio de circulação de arbovírus sugerem a Sub-região da Nhecolândia como uma importante área de vigilância para arbovírus no Centro-Oeste do Brasil. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2010-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/31296Revista do Instituto de Medicina Tropical de São Paulo; Vol. 52 No. 1 (2010); 17-24 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 52 Núm. 1 (2010); 17-24 Revista do Instituto de Medicina Tropical de São Paulo; v. 52 n. 1 (2010); 17-24 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/31296/33181Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessPauvolid-Corrêa, AlexTavares, Fernando NetoAlencar, JeronimoSilva, Julia dos SantosMurta, MicheleSerra-Freire, Nicolau MauésPellegrin, Aiesca OliveiraGil-Santana, HélcioGuimarães, Anthony ÉricoSilva, Edson Elias da2012-07-07T19:29:25Zoai:revistas.usp.br:article/31296Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:58.455459Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Preliminary investigation of Culicidae species in South Pantanal, Brazil and their potential importance in arbovirus transmission Investigação preliminar das espécies de culicídeos do Pantanal Sul brasileiro e sua potencial importância na transmissão de arbovírus |
title |
Preliminary investigation of Culicidae species in South Pantanal, Brazil and their potential importance in arbovirus transmission |
spellingShingle |
Preliminary investigation of Culicidae species in South Pantanal, Brazil and their potential importance in arbovirus transmission Pauvolid-Corrêa, Alex Arbovirus Pantanal Culicidae Ticks Nhecolândia |
title_short |
Preliminary investigation of Culicidae species in South Pantanal, Brazil and their potential importance in arbovirus transmission |
title_full |
Preliminary investigation of Culicidae species in South Pantanal, Brazil and their potential importance in arbovirus transmission |
title_fullStr |
Preliminary investigation of Culicidae species in South Pantanal, Brazil and their potential importance in arbovirus transmission |
title_full_unstemmed |
Preliminary investigation of Culicidae species in South Pantanal, Brazil and their potential importance in arbovirus transmission |
title_sort |
Preliminary investigation of Culicidae species in South Pantanal, Brazil and their potential importance in arbovirus transmission |
author |
Pauvolid-Corrêa, Alex |
author_facet |
Pauvolid-Corrêa, Alex Tavares, Fernando Neto Alencar, Jeronimo Silva, Julia dos Santos Murta, Michele Serra-Freire, Nicolau Maués Pellegrin, Aiesca Oliveira Gil-Santana, Hélcio Guimarães, Anthony Érico Silva, Edson Elias da |
author_role |
author |
author2 |
Tavares, Fernando Neto Alencar, Jeronimo Silva, Julia dos Santos Murta, Michele Serra-Freire, Nicolau Maués Pellegrin, Aiesca Oliveira Gil-Santana, Hélcio Guimarães, Anthony Érico Silva, Edson Elias da |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Pauvolid-Corrêa, Alex Tavares, Fernando Neto Alencar, Jeronimo Silva, Julia dos Santos Murta, Michele Serra-Freire, Nicolau Maués Pellegrin, Aiesca Oliveira Gil-Santana, Hélcio Guimarães, Anthony Érico Silva, Edson Elias da |
dc.subject.por.fl_str_mv |
Arbovirus Pantanal Culicidae Ticks Nhecolândia |
topic |
Arbovirus Pantanal Culicidae Ticks Nhecolândia |
description |
In view of the high circulation of migratory birds and the environmental and climatic conditions which favor the proliferation of arthropods, the Brazilian Pantanal is susceptible to circulation of arboviruses. However, the amount of data concerning arbovirus vectors in this area is scarce; therefore the aim of this study was to conduct a preliminary investigation of Culicidae species in the Nhecolândia Sub-region of South Pantanal, Brazil and their potential importance in the arbovirus transmission. A total of 3684 specimens of mosquitoes were captured, 1689 of which caught in the rainy season of 2007, were divided into 78 pools and submitted to viral isolation, Semi-Nested RT-PCR and Nested RT-PCR, with a view to identifying the most important arboviruses in Brazil. Simultaneously, 70 specimens of ticks found blood-feeding on horses were also submitted to the same virological assays. No virus was isolated and viral nucleic-acid detection by RT-PCR was also negative. Nevertheless, a total of 22 Culicidae species were identified, ten of which had previously been reported as vectors of important arboviruses. The diversity of species found blood-feeding on human and horse hosts together with the arboviruses circulation previously reported suggest that the Nhecolândia Sub-region of South Pantanal is an important area for arbovirus surveillance in Brazil. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/31296 |
url |
https://www.revistas.usp.br/rimtsp/article/view/31296 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/31296/33181 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 52 No. 1 (2010); 17-24 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 52 Núm. 1 (2010); 17-24 Revista do Instituto de Medicina Tropical de São Paulo; v. 52 n. 1 (2010); 17-24 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951647387320320 |