Diagnosis of abdominal angiostrongyliasis by PCR from sera of patients

Detalhes bibliográficos
Autor(a) principal: Silva, Ana Cristina Arámburu da
Data de Publicação: 2003
Outros Autores: Graeff-Teixeira, Carlos, Zaha, Arnaldo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/30743
Resumo: Abdominal angiostrongyliasis is a zoonotic infection caused by an intra-vascular nematode parasitic of wild rodents, Angiostrongylus costaricensis. No parasitological diagnosis is currently available and immunodiagnosis presents several drawbacks. Primers constructed based on a congeneric species, A. cantonensis, were able to amplify a 232 bp fragment from serum samples of 3 patients with histopathological diagnosis. Extraction was better performed with DNAzol and the specificity of the primers was confirmed by Southern blot. This disease has been diagnosed with frequency in south of Brazil, thus, this method appears like the important and unpublished alternative to improve diagnostic of disease.
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spelling Diagnosis of abdominal angiostrongyliasis by PCR from sera of patients Diagnóstico da angiostrongilíase abdominal utilizando PCR em soro de pacientes Angiostrongylus costaricensisEosinophilic gastroenteritisPCR Abdominal angiostrongyliasis is a zoonotic infection caused by an intra-vascular nematode parasitic of wild rodents, Angiostrongylus costaricensis. No parasitological diagnosis is currently available and immunodiagnosis presents several drawbacks. Primers constructed based on a congeneric species, A. cantonensis, were able to amplify a 232 bp fragment from serum samples of 3 patients with histopathological diagnosis. Extraction was better performed with DNAzol and the specificity of the primers was confirmed by Southern blot. This disease has been diagnosed with frequency in south of Brazil, thus, this method appears like the important and unpublished alternative to improve diagnostic of disease. Angiostrongilíase abdominal é uma infecção zoonótica causada por um parasito nematódeo intravascular de roedores silvestres, Angiostrongylus costaricensis. Nenhum diagnóstico parasitológico é atualmente disponível e o imunodiagnóstico apresenta alguns obstáculos. Oligonucleotídeos foram construídos baseados em um gênero específico, Angiostrongylus cantonensis, e foi capaz de amplificar um fragmento de 232 bp de amostras de soro de 3 pacientes com diagnóstico histopatológico. O melhor método de extração foi com DNAzol e a especificidade dos oligonucleotídeos foi confirmada por Southern Blot. A doença tem sido diagnosticada com freqüência no sul do Brasil, assim, este método surge como uma importante e inédita alternativa no auxílio do diagnóstico desta doença. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2003-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30743Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 No. 5 (2003); 295-297 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 Núm. 5 (2003); 295-297 Revista do Instituto de Medicina Tropical de São Paulo; v. 45 n. 5 (2003); 295-297 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30743/32627Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessSilva, Ana Cristina Arámburu daGraeff-Teixeira, CarlosZaha, Arnaldo2012-07-07T18:03:56Zoai:revistas.usp.br:article/30743Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:30.243702Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Diagnosis of abdominal angiostrongyliasis by PCR from sera of patients
Diagnóstico da angiostrongilíase abdominal utilizando PCR em soro de pacientes
title Diagnosis of abdominal angiostrongyliasis by PCR from sera of patients
spellingShingle Diagnosis of abdominal angiostrongyliasis by PCR from sera of patients
Silva, Ana Cristina Arámburu da
Angiostrongylus costaricensis
Eosinophilic gastroenteritis
PCR
title_short Diagnosis of abdominal angiostrongyliasis by PCR from sera of patients
title_full Diagnosis of abdominal angiostrongyliasis by PCR from sera of patients
title_fullStr Diagnosis of abdominal angiostrongyliasis by PCR from sera of patients
title_full_unstemmed Diagnosis of abdominal angiostrongyliasis by PCR from sera of patients
title_sort Diagnosis of abdominal angiostrongyliasis by PCR from sera of patients
author Silva, Ana Cristina Arámburu da
author_facet Silva, Ana Cristina Arámburu da
Graeff-Teixeira, Carlos
Zaha, Arnaldo
author_role author
author2 Graeff-Teixeira, Carlos
Zaha, Arnaldo
author2_role author
author
dc.contributor.author.fl_str_mv Silva, Ana Cristina Arámburu da
Graeff-Teixeira, Carlos
Zaha, Arnaldo
dc.subject.por.fl_str_mv Angiostrongylus costaricensis
Eosinophilic gastroenteritis
PCR
topic Angiostrongylus costaricensis
Eosinophilic gastroenteritis
PCR
description Abdominal angiostrongyliasis is a zoonotic infection caused by an intra-vascular nematode parasitic of wild rodents, Angiostrongylus costaricensis. No parasitological diagnosis is currently available and immunodiagnosis presents several drawbacks. Primers constructed based on a congeneric species, A. cantonensis, were able to amplify a 232 bp fragment from serum samples of 3 patients with histopathological diagnosis. Extraction was better performed with DNAzol and the specificity of the primers was confirmed by Southern blot. This disease has been diagnosed with frequency in south of Brazil, thus, this method appears like the important and unpublished alternative to improve diagnostic of disease.
publishDate 2003
dc.date.none.fl_str_mv 2003-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30743
url https://www.revistas.usp.br/rimtsp/article/view/30743
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30743/32627
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 No. 5 (2003); 295-297
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 Núm. 5 (2003); 295-297
Revista do Instituto de Medicina Tropical de São Paulo; v. 45 n. 5 (2003); 295-297
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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