Protocol for DNA extraction of Cryptosporidium spp. oocysts in fecal samples
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/31176 |
Resumo: | Molecular characterization of Cryptosporidium spp.oocysts in clinical samples is useful for public health since it allows the study of sources of contamination as well as the transmission in different geographical regions. Although widely used in developed countries, in Brazil it is restricted to academic studies, mostly using commercial kits for the extraction of genomic DNA, or in collaboration with external reference centers, rendering the method expensive and limited. The study proposes the application of the modifications recently introduced in the method improving feasibility with lower cost. This method was efficient for clinical samples preserved at -20 °C for up to six years and the low number of oocysts may be overcomed by repetitions of extraction. |
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Protocol for DNA extraction of Cryptosporidium spp. oocysts in fecal samples Protocolo para extração de DNA de oocistos de Cryptosporidium spp. em amostras fecais Cryptosporidium spp.DiagnosisMolecular techniques Molecular characterization of Cryptosporidium spp.oocysts in clinical samples is useful for public health since it allows the study of sources of contamination as well as the transmission in different geographical regions. Although widely used in developed countries, in Brazil it is restricted to academic studies, mostly using commercial kits for the extraction of genomic DNA, or in collaboration with external reference centers, rendering the method expensive and limited. The study proposes the application of the modifications recently introduced in the method improving feasibility with lower cost. This method was efficient for clinical samples preserved at -20 °C for up to six years and the low number of oocysts may be overcomed by repetitions of extraction. A caracterização molecular de oocistos de Cryptosporidium spp. em amostras clínicas é útil à saúde pública, pois permite estudo das fontes de contaminação e a transmissão em determinadas regiões geográficas. Apesar de largamente utilizada em países desenvolvidos, no Brasil está restrita aos estudos acadêmicos, na maioria utilizando kits comerciais para extração do DNA genômico, ou em colaborações com centros de referência externos, o que torna o método caro e limitado. Este estudo propõe a introdução de modificações nos métodos existentes para melhorar a viabilidade e baixar custos. O método proposto foi eficiente em amostras clínicas preservadas a -20 °C por até seis anos e o baixo número de oocistos pode ser contornado por replicadas extrações de DNA. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2008-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/31176Revista do Instituto de Medicina Tropical de São Paulo; Vol. 50 No. 3 (2008); 165-167 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 50 Núm. 3 (2008); 165-167 Revista do Instituto de Medicina Tropical de São Paulo; v. 50 n. 3 (2008); 165-167 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/31176/33060Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessGonçalves, Elenice M.N.Araújo, Ronalda S.Orban, MagaliMatté, Glavur R.Matté, Maria HelenaCorbett, Carlos E.P.2012-07-07T19:09:12Zoai:revistas.usp.br:article/31176Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:51.932965Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Protocol for DNA extraction of Cryptosporidium spp. oocysts in fecal samples Protocolo para extração de DNA de oocistos de Cryptosporidium spp. em amostras fecais |
title |
Protocol for DNA extraction of Cryptosporidium spp. oocysts in fecal samples |
spellingShingle |
Protocol for DNA extraction of Cryptosporidium spp. oocysts in fecal samples Gonçalves, Elenice M.N. Cryptosporidium spp. Diagnosis Molecular techniques |
title_short |
Protocol for DNA extraction of Cryptosporidium spp. oocysts in fecal samples |
title_full |
Protocol for DNA extraction of Cryptosporidium spp. oocysts in fecal samples |
title_fullStr |
Protocol for DNA extraction of Cryptosporidium spp. oocysts in fecal samples |
title_full_unstemmed |
Protocol for DNA extraction of Cryptosporidium spp. oocysts in fecal samples |
title_sort |
Protocol for DNA extraction of Cryptosporidium spp. oocysts in fecal samples |
author |
Gonçalves, Elenice M.N. |
author_facet |
Gonçalves, Elenice M.N. Araújo, Ronalda S. Orban, Magali Matté, Glavur R. Matté, Maria Helena Corbett, Carlos E.P. |
author_role |
author |
author2 |
Araújo, Ronalda S. Orban, Magali Matté, Glavur R. Matté, Maria Helena Corbett, Carlos E.P. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Gonçalves, Elenice M.N. Araújo, Ronalda S. Orban, Magali Matté, Glavur R. Matté, Maria Helena Corbett, Carlos E.P. |
dc.subject.por.fl_str_mv |
Cryptosporidium spp. Diagnosis Molecular techniques |
topic |
Cryptosporidium spp. Diagnosis Molecular techniques |
description |
Molecular characterization of Cryptosporidium spp.oocysts in clinical samples is useful for public health since it allows the study of sources of contamination as well as the transmission in different geographical regions. Although widely used in developed countries, in Brazil it is restricted to academic studies, mostly using commercial kits for the extraction of genomic DNA, or in collaboration with external reference centers, rendering the method expensive and limited. The study proposes the application of the modifications recently introduced in the method improving feasibility with lower cost. This method was efficient for clinical samples preserved at -20 °C for up to six years and the low number of oocysts may be overcomed by repetitions of extraction. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/31176 |
url |
https://www.revistas.usp.br/rimtsp/article/view/31176 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/31176/33060 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 50 No. 3 (2008); 165-167 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 50 Núm. 3 (2008); 165-167 Revista do Instituto de Medicina Tropical de São Paulo; v. 50 n. 3 (2008); 165-167 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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