Immunofluorescent antibody to rotavirus and antigen excretion in infantile acute diarrhoea

• Autor(a) principal: Nozawa, C.M.
• Data de Publicação: 1988
• Outros Autores: Lampe, E., Vaz, M.G.S., Hubinger, M.G. von
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
• Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/28606
• topic: Rotavirus antigen; Paired serology; Immunofluorescence test

A serological study was carried out to evaluate the causal relationship of acute diarrhoea and rotavirus in children under five years of age. The rotaviral infection was demonstrated in paired sera by determining the antibody titers by immunofluorescence test (IF). Bovine rotavirus-infected MA-104 cell culture was used as substratum for IF. Out of 80 paired sera it was shown that 23 (28.75%) presented seroconversion, 19 (23.75%) samples showed a twofold increase in their titers and 38 (47.5%) had no increase in rotavirus antibody. This result is discussed on the light of previously obtained results on viral antigen detection by counterimmunoelectrosmophoresis (CIEOP).