Hyperimmune antirabies sera titration by standard mouse neutralization and counterimmunoelectrophoresis tests, comparing results of different laboratories

Detalhes bibliográficos
Autor(a) principal: Díaz, Ana Maria
Data de Publicação: 1995
Outros Autores: Valentini, Elizabeth Juliana Ghiuro, Albas, Avelino, Fuches, Regina Maria Mourão, Gallina, Neuza Maria Frazatti
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/29304
Resumo: To determine the rabies antibody level of twenty-four hyperimmune equine sera, Standard Mouse Neutralization (SMN) and Couterimmunoelectrophoresis (CIE) tests were carried out, both at the Instituto Butantan (IB) and Instituto Panamericano de Protección de Alimentos y Zoonosis (INPPAZ). Statistical analysis has shown a correlation (r) of 0.9317 between the SMN and CIE performed at the IB, while at the INPPAZ it scored 0.974. Comparison of CIE data of both laboratories yielded a correlation of 0.845. The CIE technique has shown to be as sensitive and efficient as the SMN in titrating antirabies hyperimmune equine sera. Based on CIE results, a simple, rapid and inexpensive technique, tilers of sera antibody can be reliably estimated in SMN test.
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spelling Hyperimmune antirabies sera titration by standard mouse neutralization and counterimmunoelectrophoresis tests, comparing results of different laboratories Titulação de soros anti-rábicos hiperimunes, através da soroneutralização em camundongos e contraimunoeletroforese. Comparação dos resultados obtidos em diferentes laboratórios Antirabies seraCounterimmunoelectrophoresisStandard Mouse Neutralization Test To determine the rabies antibody level of twenty-four hyperimmune equine sera, Standard Mouse Neutralization (SMN) and Couterimmunoelectrophoresis (CIE) tests were carried out, both at the Instituto Butantan (IB) and Instituto Panamericano de Protección de Alimentos y Zoonosis (INPPAZ). Statistical analysis has shown a correlation (r) of 0.9317 between the SMN and CIE performed at the IB, while at the INPPAZ it scored 0.974. Comparison of CIE data of both laboratories yielded a correlation of 0.845. The CIE technique has shown to be as sensitive and efficient as the SMN in titrating antirabies hyperimmune equine sera. Based on CIE results, a simple, rapid and inexpensive technique, tilers of sera antibody can be reliably estimated in SMN test. Para a determinação do nível de anticorpos de vinte e quatro amostras de soros eqüinos hiperimunes contra a Raiva, foram utilizadas as técnicas de soroneutralização em camundongos (SN) e contraimunoeletroforese (CIE). As provas foram realizadas nos Institutos Butantan (IB) e Panamericano de Protección de Alimentos y Zoonosis (INPPAZ). A análise estatística demonstrou que para a SN e CIE feitas no IB, a correlação foi de r=0,9317, enquanto que no INPPAZ foi de r=0,974. Comparando-se os dados obtidos pela CIE nos dois laboratórios observou-se uma correlação de r=0,845. A técnica de CIE demonstrou ser tão sensível e eficiente quanto a SN na titulação de soros anti-rábicos hiperimunes de origem eqüina. Assim, com base nos resultados de CIE, que é uma técnica simples, rápida e econômica, pode-se estimar os títulos de anticorpos dos soros em SN. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1995-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/29304Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 No. 5 (1995); 435-440 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 Núm. 5 (1995); 435-440 Revista do Instituto de Medicina Tropical de São Paulo; v. 37 n. 5 (1995); 435-440 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/29304/31161Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessDíaz, Ana MariaValentini, Elizabeth Juliana GhiuroAlbas, AvelinoFuches, Regina Maria MourãoGallina, Neuza Maria Frazatti2012-07-02T01:40:51Zoai:revistas.usp.br:article/29304Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:03.163164Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Hyperimmune antirabies sera titration by standard mouse neutralization and counterimmunoelectrophoresis tests, comparing results of different laboratories
Titulação de soros anti-rábicos hiperimunes, através da soroneutralização em camundongos e contraimunoeletroforese. Comparação dos resultados obtidos em diferentes laboratórios
title Hyperimmune antirabies sera titration by standard mouse neutralization and counterimmunoelectrophoresis tests, comparing results of different laboratories
spellingShingle Hyperimmune antirabies sera titration by standard mouse neutralization and counterimmunoelectrophoresis tests, comparing results of different laboratories
Díaz, Ana Maria
Antirabies sera
Counterimmunoelectrophoresis
Standard Mouse Neutralization Test
title_short Hyperimmune antirabies sera titration by standard mouse neutralization and counterimmunoelectrophoresis tests, comparing results of different laboratories
title_full Hyperimmune antirabies sera titration by standard mouse neutralization and counterimmunoelectrophoresis tests, comparing results of different laboratories
title_fullStr Hyperimmune antirabies sera titration by standard mouse neutralization and counterimmunoelectrophoresis tests, comparing results of different laboratories
title_full_unstemmed Hyperimmune antirabies sera titration by standard mouse neutralization and counterimmunoelectrophoresis tests, comparing results of different laboratories
title_sort Hyperimmune antirabies sera titration by standard mouse neutralization and counterimmunoelectrophoresis tests, comparing results of different laboratories
author Díaz, Ana Maria
author_facet Díaz, Ana Maria
Valentini, Elizabeth Juliana Ghiuro
Albas, Avelino
Fuches, Regina Maria Mourão
Gallina, Neuza Maria Frazatti
author_role author
author2 Valentini, Elizabeth Juliana Ghiuro
Albas, Avelino
Fuches, Regina Maria Mourão
Gallina, Neuza Maria Frazatti
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Díaz, Ana Maria
Valentini, Elizabeth Juliana Ghiuro
Albas, Avelino
Fuches, Regina Maria Mourão
Gallina, Neuza Maria Frazatti
dc.subject.por.fl_str_mv Antirabies sera
Counterimmunoelectrophoresis
Standard Mouse Neutralization Test
topic Antirabies sera
Counterimmunoelectrophoresis
Standard Mouse Neutralization Test
description To determine the rabies antibody level of twenty-four hyperimmune equine sera, Standard Mouse Neutralization (SMN) and Couterimmunoelectrophoresis (CIE) tests were carried out, both at the Instituto Butantan (IB) and Instituto Panamericano de Protección de Alimentos y Zoonosis (INPPAZ). Statistical analysis has shown a correlation (r) of 0.9317 between the SMN and CIE performed at the IB, while at the INPPAZ it scored 0.974. Comparison of CIE data of both laboratories yielded a correlation of 0.845. The CIE technique has shown to be as sensitive and efficient as the SMN in titrating antirabies hyperimmune equine sera. Based on CIE results, a simple, rapid and inexpensive technique, tilers of sera antibody can be reliably estimated in SMN test.
publishDate 1995
dc.date.none.fl_str_mv 1995-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/29304
url https://www.revistas.usp.br/rimtsp/article/view/29304
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/29304/31161
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 No. 5 (1995); 435-440
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 Núm. 5 (1995); 435-440
Revista do Instituto de Medicina Tropical de São Paulo; v. 37 n. 5 (1995); 435-440
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
_version_ 1798951641663143936

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