Fluorescein method (fluorescein diacetate and ethidium bromide) to study the viability of Cryptococcus neoformans in liquor

Detalhes bibliográficos
Autor(a) principal: Corrêa, Benedito
Data de Publicação: 1990
Outros Autores: Purchio, Adhemar, Paula, Claudete R., Gambale, Walderez, Shikanai-Yasuda, Maria Aparecida
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/28707
Resumo: The utilization of the fluorescent method (fluorescein diacetate DF and ethidium bromide BE), to verify the viability of fungal cells, was studied in 40 samples of liquor, from patients with neurocryptococcosis. For removing leukocytes and red blood cells, which produce interfering fluorescence, good results were obtained with 0.3% saponin solution. After processing of liquor, 0.1 ml aliquots of resulting suspension were mixed to equal volumes of fresh DF-BE solution. The best incubation period for staining was 30 minutes, resulting in good differentiation between viable (green fluorescence) and non viable (red fluorescence) cells.
id IMT-1_d229a47820c2d46f004ec8bb42e8df51
oai_identifier_str oai:revistas.usp.br:article/28707
network_acronym_str IMT-1
network_name_str Revista do Instituto de Medicina Tropical de São Paulo
repository_id_str
spelling Fluorescein method (fluorescein diacetate and ethidium bromide) to study the viability of Cryptococcus neoformans in liquor Método fluorescente (diacetato de fluoresceína e brometo de etídeo) para o estudo da viabilidade de Cryptococcus neoformans em líquor Cryptococcus neoformansViabilidadeFluorescência The utilization of the fluorescent method (fluorescein diacetate DF and ethidium bromide BE), to verify the viability of fungal cells, was studied in 40 samples of liquor, from patients with neurocryptococcosis. For removing leukocytes and red blood cells, which produce interfering fluorescence, good results were obtained with 0.3% saponin solution. After processing of liquor, 0.1 ml aliquots of resulting suspension were mixed to equal volumes of fresh DF-BE solution. The best incubation period for staining was 30 minutes, resulting in good differentiation between viable (green fluorescence) and non viable (red fluorescence) cells. Padronizou-se método de fluorescência (solução de diacetato de fluoresceína DF e brometo de etídio BE) para análise de viabilidade de células fúngicas, em 40 amostras de liquor, provenientes de casos comprovados de neurocriptococose. A utilização de solução aquosa de saponina a 0,3% eliminou fluorescências interferentes emitidas por hemácias e leucócitos. Após o processamento dos materiais biológicos, foram retiradas alíquotas de 0,1 ml das supensões obtidas e misturadas a volumes iguais da solução DF-BE preparada pouco antes do uso. O tempo de coloração ideal foi de 30 minutos, resultando perfeita diferenciação entre microrganismos viáveis (fluorescência verde) e não viáveis (fluorescência vermelha). Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1990-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28707Revista do Instituto de Medicina Tropical de São Paulo; Vol. 32 No. 1 (1990); 46-50 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 32 Núm. 1 (1990); 46-50 Revista do Instituto de Medicina Tropical de São Paulo; v. 32 n. 1 (1990); 46-50 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTporhttps://www.revistas.usp.br/rimtsp/article/view/28707/30560Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessCorrêa, BeneditoPurchio, AdhemarPaula, Claudete R.Gambale, WalderezShikanai-Yasuda, Maria Aparecida2012-07-02T01:27:23Zoai:revistas.usp.br:article/28707Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:27.541762Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Fluorescein method (fluorescein diacetate and ethidium bromide) to study the viability of Cryptococcus neoformans in liquor
Método fluorescente (diacetato de fluoresceína e brometo de etídeo) para o estudo da viabilidade de Cryptococcus neoformans em líquor
title Fluorescein method (fluorescein diacetate and ethidium bromide) to study the viability of Cryptococcus neoformans in liquor
spellingShingle Fluorescein method (fluorescein diacetate and ethidium bromide) to study the viability of Cryptococcus neoformans in liquor
Corrêa, Benedito
Cryptococcus neoformans
Viabilidade
Fluorescência
title_short Fluorescein method (fluorescein diacetate and ethidium bromide) to study the viability of Cryptococcus neoformans in liquor
title_full Fluorescein method (fluorescein diacetate and ethidium bromide) to study the viability of Cryptococcus neoformans in liquor
title_fullStr Fluorescein method (fluorescein diacetate and ethidium bromide) to study the viability of Cryptococcus neoformans in liquor
title_full_unstemmed Fluorescein method (fluorescein diacetate and ethidium bromide) to study the viability of Cryptococcus neoformans in liquor
title_sort Fluorescein method (fluorescein diacetate and ethidium bromide) to study the viability of Cryptococcus neoformans in liquor
author Corrêa, Benedito
author_facet Corrêa, Benedito
Purchio, Adhemar
Paula, Claudete R.
Gambale, Walderez
Shikanai-Yasuda, Maria Aparecida
author_role author
author2 Purchio, Adhemar
Paula, Claudete R.
Gambale, Walderez
Shikanai-Yasuda, Maria Aparecida
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Corrêa, Benedito
Purchio, Adhemar
Paula, Claudete R.
Gambale, Walderez
Shikanai-Yasuda, Maria Aparecida
dc.subject.por.fl_str_mv Cryptococcus neoformans
Viabilidade
Fluorescência
topic Cryptococcus neoformans
Viabilidade
Fluorescência
description The utilization of the fluorescent method (fluorescein diacetate DF and ethidium bromide BE), to verify the viability of fungal cells, was studied in 40 samples of liquor, from patients with neurocryptococcosis. For removing leukocytes and red blood cells, which produce interfering fluorescence, good results were obtained with 0.3% saponin solution. After processing of liquor, 0.1 ml aliquots of resulting suspension were mixed to equal volumes of fresh DF-BE solution. The best incubation period for staining was 30 minutes, resulting in good differentiation between viable (green fluorescence) and non viable (red fluorescence) cells.
publishDate 1990
dc.date.none.fl_str_mv 1990-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28707
url https://www.revistas.usp.br/rimtsp/article/view/28707
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28707/30560
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 32 No. 1 (1990); 46-50
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 32 Núm. 1 (1990); 46-50
Revista do Instituto de Medicina Tropical de São Paulo; v. 32 n. 1 (1990); 46-50
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
_version_ 1798951638285680640

Registros relacionados