Isolation of yellow fever virus (YFV) from naturally infectied Haemagogus (Conopostegus) leucocelaenus (diptera, cukicudae) in São Paulo State, Brazil, 2009
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/31392 |
Resumo: | After detecting the death of Howlers monkeys (genus Alouatta) and isolation of yellow fever virus (YFV) in Buri county, São Paulo, Brazil, an entomological research study in the field was started. A YFV strain was isolated from newborn Swiss mice and cultured cells of Aedes albopictus - C6/36, from a pool of six Haemagogus (Conopostegus) leucocelaenus (Hg. leucocelaenus) mosquitoes (Dyar & Shannon) collected at the study site. Virus RNA fragment was amplified by RT-PCR and sequenced. The MCC Tree generated showed that the isolated strain is related to the South American I genotype, in a monophyletic clade containing isolates from recent 2008-2010 epidemics and epizootics in Brazil. Statistical analysis commonly used were calculated to characterize the sample in relation to diversity and dominance and indicated a pattern of dominance of one or a few species. Hg. leucocelaenus was found infected in Rio Grande do Sul State as well. In São Paulo State, this is the first detection of YFV in Hg. leucocelaenus. |
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Isolation of yellow fever virus (YFV) from naturally infectied Haemagogus (Conopostegus) leucocelaenus (diptera, cukicudae) in São Paulo State, Brazil, 2009 Isolamento do vírus da Febre Amarela de mosquitos naturalmente infectados Haemagogus (Conopostegus) leucocelaenus (Diptera, Culicidae), São Paulo, Brasil, 2009 Yellow feverHaemagogus leucocelaenusArbovirus After detecting the death of Howlers monkeys (genus Alouatta) and isolation of yellow fever virus (YFV) in Buri county, São Paulo, Brazil, an entomological research study in the field was started. A YFV strain was isolated from newborn Swiss mice and cultured cells of Aedes albopictus - C6/36, from a pool of six Haemagogus (Conopostegus) leucocelaenus (Hg. leucocelaenus) mosquitoes (Dyar & Shannon) collected at the study site. Virus RNA fragment was amplified by RT-PCR and sequenced. The MCC Tree generated showed that the isolated strain is related to the South American I genotype, in a monophyletic clade containing isolates from recent 2008-2010 epidemics and epizootics in Brazil. Statistical analysis commonly used were calculated to characterize the sample in relation to diversity and dominance and indicated a pattern of dominance of one or a few species. Hg. leucocelaenus was found infected in Rio Grande do Sul State as well. In São Paulo State, this is the first detection of YFV in Hg. leucocelaenus. Após a detecção de morte de macacos Bugios (gênero Alouatta) e isolamento do vírus da Febre Amarela (YFV) no município de Buri, Estado de São Paulo, Brasil, foi iniciada uma investigação entomológica em campo. Uma cepa de YFV foi isolada em camundongos recém-nascidos e cultura de células de Aedes albopictus - C6/36, a partir de um lote de seis mosquitos Haemagogus (Conopostegus) leucocelaenus (Hg leucocelaenus) Dyar & Shannon coletados no local de estudo. RNA do vírus foi amplificado por RT-PCR e seqüenciado. A topologia gerada indica que a cepa isolada está relacionada ao genótipo South American I, em clado monofilético englobando isolados recentes de epidemias e epizootias entre 2008 e 2009. Análises estatísticas geralmente usadas caracterizaram a amostra em relação à diversidade e dominância, indicando dominância relativa de uma ou poucas espécies. Hg. leucocelaenus foi detectado infectado também no Rio Grande do Sul. No Estado de São Paulo trata-se da primeira detecção do YFV em Hg leucocelaenus. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2011-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/31392Revista do Instituto de Medicina Tropical de São Paulo; Vol. 53 No. 3 (2011); 133-139 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 53 Núm. 3 (2011); 133-139 Revista do Instituto de Medicina Tropical de São Paulo; v. 53 n. 3 (2011); 133-139 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/31392/33277Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessSouza, Renato Pereira dePetrella, SelmaCoimbra, Terezinha Lisieux MoraesMaeda, Adriana YurikaRocco, Iray MariaBisordi, IvaniSilveira, Vivian ReginaPereira, Luiz EloySuzuki, AkemiSilva, Sarai Joaquim dos SantosSilva, Fernanda GiseleSalvador, Felipe ScassiTubaki, Rosa MariaMenezes, Regiane TironiPereira, MarizaBergo, Eduardo SterlinoHoffmann, Roberto ColozzaSpinola, Roberta Maria FernandesTengan, Cílea HatsumiSiciliano, Melissa Mascheratti2012-07-07T19:39:40Zoai:revistas.usp.br:article/31392Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:04.053591Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Isolation of yellow fever virus (YFV) from naturally infectied Haemagogus (Conopostegus) leucocelaenus (diptera, cukicudae) in São Paulo State, Brazil, 2009 Isolamento do vírus da Febre Amarela de mosquitos naturalmente infectados Haemagogus (Conopostegus) leucocelaenus (Diptera, Culicidae), São Paulo, Brasil, 2009 |
title |
Isolation of yellow fever virus (YFV) from naturally infectied Haemagogus (Conopostegus) leucocelaenus (diptera, cukicudae) in São Paulo State, Brazil, 2009 |
spellingShingle |
Isolation of yellow fever virus (YFV) from naturally infectied Haemagogus (Conopostegus) leucocelaenus (diptera, cukicudae) in São Paulo State, Brazil, 2009 Souza, Renato Pereira de Yellow fever Haemagogus leucocelaenus Arbovirus |
title_short |
Isolation of yellow fever virus (YFV) from naturally infectied Haemagogus (Conopostegus) leucocelaenus (diptera, cukicudae) in São Paulo State, Brazil, 2009 |
title_full |
Isolation of yellow fever virus (YFV) from naturally infectied Haemagogus (Conopostegus) leucocelaenus (diptera, cukicudae) in São Paulo State, Brazil, 2009 |
title_fullStr |
Isolation of yellow fever virus (YFV) from naturally infectied Haemagogus (Conopostegus) leucocelaenus (diptera, cukicudae) in São Paulo State, Brazil, 2009 |
title_full_unstemmed |
Isolation of yellow fever virus (YFV) from naturally infectied Haemagogus (Conopostegus) leucocelaenus (diptera, cukicudae) in São Paulo State, Brazil, 2009 |
title_sort |
Isolation of yellow fever virus (YFV) from naturally infectied Haemagogus (Conopostegus) leucocelaenus (diptera, cukicudae) in São Paulo State, Brazil, 2009 |
author |
Souza, Renato Pereira de |
author_facet |
Souza, Renato Pereira de Petrella, Selma Coimbra, Terezinha Lisieux Moraes Maeda, Adriana Yurika Rocco, Iray Maria Bisordi, Ivani Silveira, Vivian Regina Pereira, Luiz Eloy Suzuki, Akemi Silva, Sarai Joaquim dos Santos Silva, Fernanda Gisele Salvador, Felipe Scassi Tubaki, Rosa Maria Menezes, Regiane Tironi Pereira, Mariza Bergo, Eduardo Sterlino Hoffmann, Roberto Colozza Spinola, Roberta Maria Fernandes Tengan, Cílea Hatsumi Siciliano, Melissa Mascheratti |
author_role |
author |
author2 |
Petrella, Selma Coimbra, Terezinha Lisieux Moraes Maeda, Adriana Yurika Rocco, Iray Maria Bisordi, Ivani Silveira, Vivian Regina Pereira, Luiz Eloy Suzuki, Akemi Silva, Sarai Joaquim dos Santos Silva, Fernanda Gisele Salvador, Felipe Scassi Tubaki, Rosa Maria Menezes, Regiane Tironi Pereira, Mariza Bergo, Eduardo Sterlino Hoffmann, Roberto Colozza Spinola, Roberta Maria Fernandes Tengan, Cílea Hatsumi Siciliano, Melissa Mascheratti |
author2_role |
author author author author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Souza, Renato Pereira de Petrella, Selma Coimbra, Terezinha Lisieux Moraes Maeda, Adriana Yurika Rocco, Iray Maria Bisordi, Ivani Silveira, Vivian Regina Pereira, Luiz Eloy Suzuki, Akemi Silva, Sarai Joaquim dos Santos Silva, Fernanda Gisele Salvador, Felipe Scassi Tubaki, Rosa Maria Menezes, Regiane Tironi Pereira, Mariza Bergo, Eduardo Sterlino Hoffmann, Roberto Colozza Spinola, Roberta Maria Fernandes Tengan, Cílea Hatsumi Siciliano, Melissa Mascheratti |
dc.subject.por.fl_str_mv |
Yellow fever Haemagogus leucocelaenus Arbovirus |
topic |
Yellow fever Haemagogus leucocelaenus Arbovirus |
description |
After detecting the death of Howlers monkeys (genus Alouatta) and isolation of yellow fever virus (YFV) in Buri county, São Paulo, Brazil, an entomological research study in the field was started. A YFV strain was isolated from newborn Swiss mice and cultured cells of Aedes albopictus - C6/36, from a pool of six Haemagogus (Conopostegus) leucocelaenus (Hg. leucocelaenus) mosquitoes (Dyar & Shannon) collected at the study site. Virus RNA fragment was amplified by RT-PCR and sequenced. The MCC Tree generated showed that the isolated strain is related to the South American I genotype, in a monophyletic clade containing isolates from recent 2008-2010 epidemics and epizootics in Brazil. Statistical analysis commonly used were calculated to characterize the sample in relation to diversity and dominance and indicated a pattern of dominance of one or a few species. Hg. leucocelaenus was found infected in Rio Grande do Sul State as well. In São Paulo State, this is the first detection of YFV in Hg. leucocelaenus. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/31392 |
url |
https://www.revistas.usp.br/rimtsp/article/view/31392 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/31392/33277 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 53 No. 3 (2011); 133-139 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 53 Núm. 3 (2011); 133-139 Revista do Instituto de Medicina Tropical de São Paulo; v. 53 n. 3 (2011); 133-139 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951647962988544 |