Simplified fluorescent inhibition microtest for the titration of rabies neutralizing antibodies
Autor(a) principal: | |
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Data de Publicação: | 1993 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/29020 |
Resumo: | A simplified fluorescence inhibition microtest (SFIMT) was standardized for the evaluation of antirabies serum neutralizing antibodies based on the rapid fluorescent focus inhibition test (RFFIT) and the fluorescence inhibition microtest (FIMT). The simplified test showed reproductibility similar to that of the FIMT with advantages as easier executation and quicker reading. A simple pre-treatment of Brazilian microplates produced for immune enzymatic assays (PROSIL) gave equivalent results and substantial coast reduction, in relation to imported plates (DIFCO). The simplified test can be easily implemented in less sophisticated laboratories, as alternative to the mouse serum neutralization test, still the most largely employed in Brazil, or even to others as RFFIT and FIMT. |
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Simplified fluorescent inhibition microtest for the titration of rabies neutralizing antibodies Microteste de inibição de fluorescência simplificado para titulação de anticorpos antirábicos Simplified Fluorescence Inhibition Microtestrabies antibodies A simplified fluorescence inhibition microtest (SFIMT) was standardized for the evaluation of antirabies serum neutralizing antibodies based on the rapid fluorescent focus inhibition test (RFFIT) and the fluorescence inhibition microtest (FIMT). The simplified test showed reproductibility similar to that of the FIMT with advantages as easier executation and quicker reading. A simple pre-treatment of Brazilian microplates produced for immune enzymatic assays (PROSIL) gave equivalent results and substantial coast reduction, in relation to imported plates (DIFCO). The simplified test can be easily implemented in less sophisticated laboratories, as alternative to the mouse serum neutralization test, still the most largely employed in Brazil, or even to others as RFFIT and FIMT. No presente trabalho foi padronizada uma microtécnica simplificada para a avaliação de anticorpos neutralizantes anti-rábicos com base no RFFIT e no FIMT. Este teste mostrou reprodutibilidade comparável a dos testes originais, sendo de execução mais simples e de leitura mais rápida. Uma microplaca de fabricação nacional para testes imuno-enzimáticos (PROSIL Ind. e Com.) pode ser utilizada com resultados equivalentes aos de microplacas Difco ou similar, com substancial redução de custos, mediante tratamento simples e rápido. O teste estudado pode ser implantado facilmente em laboratórios menos sofisticados substituindo com grandes vantagens a prova de soro-neutralização em camundongos ainda a mais empregada rotineiramente no Brasil. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1993-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/29020Revista do Instituto de Medicina Tropical de São Paulo; Vol. 35 No. 2 (1993); 171-175 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 35 Núm. 2 (1993); 171-175 Revista do Instituto de Medicina Tropical de São Paulo; v. 35 n. 2 (1993); 171-175 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/29020/30877Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessFavoretto, S.R.Carrieri, M.L.Tino, M.S.Zanetti, C.R.Pereira, O.A.C.2012-07-02T01:35:13Zoai:revistas.usp.br:article/29020Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:46.792186Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Simplified fluorescent inhibition microtest for the titration of rabies neutralizing antibodies Microteste de inibição de fluorescência simplificado para titulação de anticorpos antirábicos |
title |
Simplified fluorescent inhibition microtest for the titration of rabies neutralizing antibodies |
spellingShingle |
Simplified fluorescent inhibition microtest for the titration of rabies neutralizing antibodies Favoretto, S.R. Simplified Fluorescence Inhibition Microtest rabies antibodies |
title_short |
Simplified fluorescent inhibition microtest for the titration of rabies neutralizing antibodies |
title_full |
Simplified fluorescent inhibition microtest for the titration of rabies neutralizing antibodies |
title_fullStr |
Simplified fluorescent inhibition microtest for the titration of rabies neutralizing antibodies |
title_full_unstemmed |
Simplified fluorescent inhibition microtest for the titration of rabies neutralizing antibodies |
title_sort |
Simplified fluorescent inhibition microtest for the titration of rabies neutralizing antibodies |
author |
Favoretto, S.R. |
author_facet |
Favoretto, S.R. Carrieri, M.L. Tino, M.S. Zanetti, C.R. Pereira, O.A.C. |
author_role |
author |
author2 |
Carrieri, M.L. Tino, M.S. Zanetti, C.R. Pereira, O.A.C. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Favoretto, S.R. Carrieri, M.L. Tino, M.S. Zanetti, C.R. Pereira, O.A.C. |
dc.subject.por.fl_str_mv |
Simplified Fluorescence Inhibition Microtest rabies antibodies |
topic |
Simplified Fluorescence Inhibition Microtest rabies antibodies |
description |
A simplified fluorescence inhibition microtest (SFIMT) was standardized for the evaluation of antirabies serum neutralizing antibodies based on the rapid fluorescent focus inhibition test (RFFIT) and the fluorescence inhibition microtest (FIMT). The simplified test showed reproductibility similar to that of the FIMT with advantages as easier executation and quicker reading. A simple pre-treatment of Brazilian microplates produced for immune enzymatic assays (PROSIL) gave equivalent results and substantial coast reduction, in relation to imported plates (DIFCO). The simplified test can be easily implemented in less sophisticated laboratories, as alternative to the mouse serum neutralization test, still the most largely employed in Brazil, or even to others as RFFIT and FIMT. |
publishDate |
1993 |
dc.date.none.fl_str_mv |
1993-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/29020 |
url |
https://www.revistas.usp.br/rimtsp/article/view/29020 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/29020/30877 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 35 No. 2 (1993); 171-175 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 35 Núm. 2 (1993); 171-175 Revista do Instituto de Medicina Tropical de São Paulo; v. 35 n. 2 (1993); 171-175 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
_version_ |
1798951640003248128 |