The interplay between mutations in cagA, 23S rRNA, gyrA and drug resistance in Helicobacter pylori

Detalhes bibliográficos
Autor(a) principal: Vianna, Júlia Silveira
Data de Publicação: 2018
Outros Autores: Ramis, Ivy Bastos, Ramos, Daniela Fernandes, Gastal, Otávio Leite, Silva, Renato Azevedo da, Gonçalves, Carla Vitola, Silva, Pedro Eduardo Almeida da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/148082
Resumo: In this study, we evaluated the mutations of Helicobacter pylori associated with resistance to clarithromycin and levofloxacin. Furthermore, based on the proposed interaction between antimicrobial resistance and pathogenicity, we correlated the mutation profiles of the strains with the presence of the pathogenicity gene cagA. We analyzed 80 gastric biopsy specimens from H. pylori-infected patients for point mutations in the 23S rRNA gene region and in the gyrA gene, which are related to clarithromycin and levofloxacin resistance, respectively, and investigated the presence of the cagA gene in these strains. We observed that in the assayed biopsies, 8.7% (7/80) had mutations in the 23S rRNA gene region at positions 2143 and 2142, while 22.5% (18/80) had mutations in gyrA at codons 87 and 91. Moreover, absence of the CagA-EPIYA pathogenicity factor was observed in 68% (17/25) of resistant samples. The knowledge of the local profile of antimicrobial resistance and the complex interplay involving resistance and pathogenicity can contribute to an appropriate clinical approach.
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spelling The interplay between mutations in cagA, 23S rRNA, gyrA and drug resistance in Helicobacter pyloriAntimicrobial resistancePoint mutationsClarithromycinLevofloxacinCagA EPIYA In this study, we evaluated the mutations of Helicobacter pylori associated with resistance to clarithromycin and levofloxacin. Furthermore, based on the proposed interaction between antimicrobial resistance and pathogenicity, we correlated the mutation profiles of the strains with the presence of the pathogenicity gene cagA. We analyzed 80 gastric biopsy specimens from H. pylori-infected patients for point mutations in the 23S rRNA gene region and in the gyrA gene, which are related to clarithromycin and levofloxacin resistance, respectively, and investigated the presence of the cagA gene in these strains. We observed that in the assayed biopsies, 8.7% (7/80) had mutations in the 23S rRNA gene region at positions 2143 and 2142, while 22.5% (18/80) had mutations in gyrA at codons 87 and 91. Moreover, absence of the CagA-EPIYA pathogenicity factor was observed in 68% (17/25) of resistant samples. The knowledge of the local profile of antimicrobial resistance and the complex interplay involving resistance and pathogenicity can contribute to an appropriate clinical approach.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/xmlhttps://www.revistas.usp.br/rimtsp/article/view/148082Revista do Instituto de Medicina Tropical de São Paulo; Vol. 60 (2018); e25Revista do Instituto de Medicina Tropical de São Paulo; Vol. 60 (2018); e25Revista do Instituto de Medicina Tropical de São Paulo; v. 60 (2018); e251678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/148082/141656https://www.revistas.usp.br/rimtsp/article/view/148082/148547Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessVianna, Júlia SilveiraRamis, Ivy BastosRamos, Daniela FernandesGastal, Otávio LeiteSilva, Renato Azevedo daGonçalves, Carla VitolaSilva, Pedro Eduardo Almeida da2018-07-12T16:41:34Zoai:revistas.usp.br:article/148082Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:44.862333Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv The interplay between mutations in cagA, 23S rRNA, gyrA and drug resistance in Helicobacter pylori
title The interplay between mutations in cagA, 23S rRNA, gyrA and drug resistance in Helicobacter pylori
spellingShingle The interplay between mutations in cagA, 23S rRNA, gyrA and drug resistance in Helicobacter pylori
Vianna, Júlia Silveira
Antimicrobial resistance
Point mutations
Clarithromycin
Levofloxacin
CagA EPIYA
title_short The interplay between mutations in cagA, 23S rRNA, gyrA and drug resistance in Helicobacter pylori
title_full The interplay between mutations in cagA, 23S rRNA, gyrA and drug resistance in Helicobacter pylori
title_fullStr The interplay between mutations in cagA, 23S rRNA, gyrA and drug resistance in Helicobacter pylori
title_full_unstemmed The interplay between mutations in cagA, 23S rRNA, gyrA and drug resistance in Helicobacter pylori
title_sort The interplay between mutations in cagA, 23S rRNA, gyrA and drug resistance in Helicobacter pylori
author Vianna, Júlia Silveira
author_facet Vianna, Júlia Silveira
Ramis, Ivy Bastos
Ramos, Daniela Fernandes
Gastal, Otávio Leite
Silva, Renato Azevedo da
Gonçalves, Carla Vitola
Silva, Pedro Eduardo Almeida da
author_role author
author2 Ramis, Ivy Bastos
Ramos, Daniela Fernandes
Gastal, Otávio Leite
Silva, Renato Azevedo da
Gonçalves, Carla Vitola
Silva, Pedro Eduardo Almeida da
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vianna, Júlia Silveira
Ramis, Ivy Bastos
Ramos, Daniela Fernandes
Gastal, Otávio Leite
Silva, Renato Azevedo da
Gonçalves, Carla Vitola
Silva, Pedro Eduardo Almeida da
dc.subject.por.fl_str_mv Antimicrobial resistance
Point mutations
Clarithromycin
Levofloxacin
CagA EPIYA
topic Antimicrobial resistance
Point mutations
Clarithromycin
Levofloxacin
CagA EPIYA
description In this study, we evaluated the mutations of Helicobacter pylori associated with resistance to clarithromycin and levofloxacin. Furthermore, based on the proposed interaction between antimicrobial resistance and pathogenicity, we correlated the mutation profiles of the strains with the presence of the pathogenicity gene cagA. We analyzed 80 gastric biopsy specimens from H. pylori-infected patients for point mutations in the 23S rRNA gene region and in the gyrA gene, which are related to clarithromycin and levofloxacin resistance, respectively, and investigated the presence of the cagA gene in these strains. We observed that in the assayed biopsies, 8.7% (7/80) had mutations in the 23S rRNA gene region at positions 2143 and 2142, while 22.5% (18/80) had mutations in gyrA at codons 87 and 91. Moreover, absence of the CagA-EPIYA pathogenicity factor was observed in 68% (17/25) of resistant samples. The knowledge of the local profile of antimicrobial resistance and the complex interplay involving resistance and pathogenicity can contribute to an appropriate clinical approach.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/148082
url https://www.revistas.usp.br/rimtsp/article/view/148082
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/148082/141656
https://www.revistas.usp.br/rimtsp/article/view/148082/148547
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/xml
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 60 (2018); e25
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 60 (2018); e25
Revista do Instituto de Medicina Tropical de São Paulo; v. 60 (2018); e25
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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