The interplay between mutations in cagA, 23S rRNA, gyrA and drug resistance in Helicobacter pylori
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/148082 |
Resumo: | In this study, we evaluated the mutations of Helicobacter pylori associated with resistance to clarithromycin and levofloxacin. Furthermore, based on the proposed interaction between antimicrobial resistance and pathogenicity, we correlated the mutation profiles of the strains with the presence of the pathogenicity gene cagA. We analyzed 80 gastric biopsy specimens from H. pylori-infected patients for point mutations in the 23S rRNA gene region and in the gyrA gene, which are related to clarithromycin and levofloxacin resistance, respectively, and investigated the presence of the cagA gene in these strains. We observed that in the assayed biopsies, 8.7% (7/80) had mutations in the 23S rRNA gene region at positions 2143 and 2142, while 22.5% (18/80) had mutations in gyrA at codons 87 and 91. Moreover, absence of the CagA-EPIYA pathogenicity factor was observed in 68% (17/25) of resistant samples. The knowledge of the local profile of antimicrobial resistance and the complex interplay involving resistance and pathogenicity can contribute to an appropriate clinical approach. |
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Revista do Instituto de Medicina Tropical de São Paulo |
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The interplay between mutations in cagA, 23S rRNA, gyrA and drug resistance in Helicobacter pyloriAntimicrobial resistancePoint mutationsClarithromycinLevofloxacinCagA EPIYA In this study, we evaluated the mutations of Helicobacter pylori associated with resistance to clarithromycin and levofloxacin. Furthermore, based on the proposed interaction between antimicrobial resistance and pathogenicity, we correlated the mutation profiles of the strains with the presence of the pathogenicity gene cagA. We analyzed 80 gastric biopsy specimens from H. pylori-infected patients for point mutations in the 23S rRNA gene region and in the gyrA gene, which are related to clarithromycin and levofloxacin resistance, respectively, and investigated the presence of the cagA gene in these strains. We observed that in the assayed biopsies, 8.7% (7/80) had mutations in the 23S rRNA gene region at positions 2143 and 2142, while 22.5% (18/80) had mutations in gyrA at codons 87 and 91. Moreover, absence of the CagA-EPIYA pathogenicity factor was observed in 68% (17/25) of resistant samples. The knowledge of the local profile of antimicrobial resistance and the complex interplay involving resistance and pathogenicity can contribute to an appropriate clinical approach.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/xmlhttps://www.revistas.usp.br/rimtsp/article/view/148082Revista do Instituto de Medicina Tropical de São Paulo; Vol. 60 (2018); e25Revista do Instituto de Medicina Tropical de São Paulo; Vol. 60 (2018); e25Revista do Instituto de Medicina Tropical de São Paulo; v. 60 (2018); e251678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/148082/141656https://www.revistas.usp.br/rimtsp/article/view/148082/148547Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessVianna, Júlia SilveiraRamis, Ivy BastosRamos, Daniela FernandesGastal, Otávio LeiteSilva, Renato Azevedo daGonçalves, Carla VitolaSilva, Pedro Eduardo Almeida da2018-07-12T16:41:34Zoai:revistas.usp.br:article/148082Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:44.862333Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
The interplay between mutations in cagA, 23S rRNA, gyrA and drug resistance in Helicobacter pylori |
title |
The interplay between mutations in cagA, 23S rRNA, gyrA and drug resistance in Helicobacter pylori |
spellingShingle |
The interplay between mutations in cagA, 23S rRNA, gyrA and drug resistance in Helicobacter pylori Vianna, Júlia Silveira Antimicrobial resistance Point mutations Clarithromycin Levofloxacin CagA EPIYA |
title_short |
The interplay between mutations in cagA, 23S rRNA, gyrA and drug resistance in Helicobacter pylori |
title_full |
The interplay between mutations in cagA, 23S rRNA, gyrA and drug resistance in Helicobacter pylori |
title_fullStr |
The interplay between mutations in cagA, 23S rRNA, gyrA and drug resistance in Helicobacter pylori |
title_full_unstemmed |
The interplay between mutations in cagA, 23S rRNA, gyrA and drug resistance in Helicobacter pylori |
title_sort |
The interplay between mutations in cagA, 23S rRNA, gyrA and drug resistance in Helicobacter pylori |
author |
Vianna, Júlia Silveira |
author_facet |
Vianna, Júlia Silveira Ramis, Ivy Bastos Ramos, Daniela Fernandes Gastal, Otávio Leite Silva, Renato Azevedo da Gonçalves, Carla Vitola Silva, Pedro Eduardo Almeida da |
author_role |
author |
author2 |
Ramis, Ivy Bastos Ramos, Daniela Fernandes Gastal, Otávio Leite Silva, Renato Azevedo da Gonçalves, Carla Vitola Silva, Pedro Eduardo Almeida da |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Vianna, Júlia Silveira Ramis, Ivy Bastos Ramos, Daniela Fernandes Gastal, Otávio Leite Silva, Renato Azevedo da Gonçalves, Carla Vitola Silva, Pedro Eduardo Almeida da |
dc.subject.por.fl_str_mv |
Antimicrobial resistance Point mutations Clarithromycin Levofloxacin CagA EPIYA |
topic |
Antimicrobial resistance Point mutations Clarithromycin Levofloxacin CagA EPIYA |
description |
In this study, we evaluated the mutations of Helicobacter pylori associated with resistance to clarithromycin and levofloxacin. Furthermore, based on the proposed interaction between antimicrobial resistance and pathogenicity, we correlated the mutation profiles of the strains with the presence of the pathogenicity gene cagA. We analyzed 80 gastric biopsy specimens from H. pylori-infected patients for point mutations in the 23S rRNA gene region and in the gyrA gene, which are related to clarithromycin and levofloxacin resistance, respectively, and investigated the presence of the cagA gene in these strains. We observed that in the assayed biopsies, 8.7% (7/80) had mutations in the 23S rRNA gene region at positions 2143 and 2142, while 22.5% (18/80) had mutations in gyrA at codons 87 and 91. Moreover, absence of the CagA-EPIYA pathogenicity factor was observed in 68% (17/25) of resistant samples. The knowledge of the local profile of antimicrobial resistance and the complex interplay involving resistance and pathogenicity can contribute to an appropriate clinical approach. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/148082 |
url |
https://www.revistas.usp.br/rimtsp/article/view/148082 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/148082/141656 https://www.revistas.usp.br/rimtsp/article/view/148082/148547 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/xml |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 60 (2018); e25 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 60 (2018); e25 Revista do Instituto de Medicina Tropical de São Paulo; v. 60 (2018); e25 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951652139466752 |