Low occurrence of Hepatitis A virus in water samples from an urban area of Southern Brazil

Detalhes bibliográficos
Autor(a) principal: Souza, Fernanda Gil de
Data de Publicação: 2018
Outros Autores: Silva, Francini Pereira da, Staggemeier, Rodrigo, Rigotto, Caroline, Spilki, Fernando Rosado
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/151628
Resumo: Hepatitis A virus (HAV), a member of Picornaviridae family, is the main causative agent of acute viral hepatitis in the world, mainly in developing countries. HAV may be present in contaminated water and food and its presence is often associated to a lesser extent with socioeconomic factors and environmental quality. The main goals in the present study were to standardize a cell culture combined to a polymerase chain reaction protocol for the detection and quantification of viral viability and analyze whether the virus could be found in water samples collected in four urban streams of Sinos River watershed. Virus recovery was assayed from known virus concentrations measured in experimentally contaminated raw and ultrapure water (MilliQ®). Recovery rates ranged from 270% in raw water to 15,000% in ultrapure water. In a second step, a qPCR coupled to a previous passage in cells, demonstrated more analytical sensitivity when compared to samples assayed without a previous passage in cell cultures. HAV genome was detected in only 1 of 84 samples analyzed, pointing to a very low occurrence of HAV in water samples in the studied region. These findings are remarkable, since no more than 5% of the domestic sewage in this area is treated pointing to a low occurrence of HAV in the population living nearby during the study period.
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spelling Low occurrence of Hepatitis A virus in water samples from an urban area of Southern BrazilHAVStreamICC-PCRRT-PCRHepatitisNested-RT-PCREnteric virusHepatitis A virus (HAV), a member of Picornaviridae family, is the main causative agent of acute viral hepatitis in the world, mainly in developing countries. HAV may be present in contaminated water and food and its presence is often associated to a lesser extent with socioeconomic factors and environmental quality. The main goals in the present study were to standardize a cell culture combined to a polymerase chain reaction protocol for the detection and quantification of viral viability and analyze whether the virus could be found in water samples collected in four urban streams of Sinos River watershed. Virus recovery was assayed from known virus concentrations measured in experimentally contaminated raw and ultrapure water (MilliQ®). Recovery rates ranged from 270% in raw water to 15,000% in ultrapure water. In a second step, a qPCR coupled to a previous passage in cells, demonstrated more analytical sensitivity when compared to samples assayed without a previous passage in cell cultures. HAV genome was detected in only 1 of 84 samples analyzed, pointing to a very low occurrence of HAV in water samples in the studied region. These findings are remarkable, since no more than 5% of the domestic sewage in this area is treated pointing to a low occurrence of HAV in the population living nearby during the study period.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2018-11-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/xmlhttps://www.revistas.usp.br/rimtsp/article/view/151628Revista do Instituto de Medicina Tropical de São Paulo; Vol. 60 (2018); e69Revista do Instituto de Medicina Tropical de São Paulo; Vol. 60 (2018); e69Revista do Instituto de Medicina Tropical de São Paulo; v. 60 (2018); e691678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/151628/148577https://www.revistas.usp.br/rimtsp/article/view/151628/148578Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessSouza, Fernanda Gil deSilva, Francini Pereira daStaggemeier, RodrigoRigotto, CarolineSpilki, Fernando Rosado2018-11-08T12:21:38Zoai:revistas.usp.br:article/151628Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:45.615084Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Low occurrence of Hepatitis A virus in water samples from an urban area of Southern Brazil
title Low occurrence of Hepatitis A virus in water samples from an urban area of Southern Brazil
spellingShingle Low occurrence of Hepatitis A virus in water samples from an urban area of Southern Brazil
Souza, Fernanda Gil de
HAV
Stream
ICC-PCR
RT-PCR
Hepatitis
Nested-RT-PCR
Enteric virus
title_short Low occurrence of Hepatitis A virus in water samples from an urban area of Southern Brazil
title_full Low occurrence of Hepatitis A virus in water samples from an urban area of Southern Brazil
title_fullStr Low occurrence of Hepatitis A virus in water samples from an urban area of Southern Brazil
title_full_unstemmed Low occurrence of Hepatitis A virus in water samples from an urban area of Southern Brazil
title_sort Low occurrence of Hepatitis A virus in water samples from an urban area of Southern Brazil
author Souza, Fernanda Gil de
author_facet Souza, Fernanda Gil de
Silva, Francini Pereira da
Staggemeier, Rodrigo
Rigotto, Caroline
Spilki, Fernando Rosado
author_role author
author2 Silva, Francini Pereira da
Staggemeier, Rodrigo
Rigotto, Caroline
Spilki, Fernando Rosado
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Souza, Fernanda Gil de
Silva, Francini Pereira da
Staggemeier, Rodrigo
Rigotto, Caroline
Spilki, Fernando Rosado
dc.subject.por.fl_str_mv HAV
Stream
ICC-PCR
RT-PCR
Hepatitis
Nested-RT-PCR
Enteric virus
topic HAV
Stream
ICC-PCR
RT-PCR
Hepatitis
Nested-RT-PCR
Enteric virus
description Hepatitis A virus (HAV), a member of Picornaviridae family, is the main causative agent of acute viral hepatitis in the world, mainly in developing countries. HAV may be present in contaminated water and food and its presence is often associated to a lesser extent with socioeconomic factors and environmental quality. The main goals in the present study were to standardize a cell culture combined to a polymerase chain reaction protocol for the detection and quantification of viral viability and analyze whether the virus could be found in water samples collected in four urban streams of Sinos River watershed. Virus recovery was assayed from known virus concentrations measured in experimentally contaminated raw and ultrapure water (MilliQ®). Recovery rates ranged from 270% in raw water to 15,000% in ultrapure water. In a second step, a qPCR coupled to a previous passage in cells, demonstrated more analytical sensitivity when compared to samples assayed without a previous passage in cell cultures. HAV genome was detected in only 1 of 84 samples analyzed, pointing to a very low occurrence of HAV in water samples in the studied region. These findings are remarkable, since no more than 5% of the domestic sewage in this area is treated pointing to a low occurrence of HAV in the population living nearby during the study period.
publishDate 2018
dc.date.none.fl_str_mv 2018-11-08
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/151628
url https://www.revistas.usp.br/rimtsp/article/view/151628
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/151628/148577
https://www.revistas.usp.br/rimtsp/article/view/151628/148578
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/xml
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 60 (2018); e69
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 60 (2018); e69
Revista do Instituto de Medicina Tropical de São Paulo; v. 60 (2018); e69
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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