Characterization of Leishmania (L.) infantum chagasi in visceral leishmaniasis associated with hiv co-infection in Northeastern Brazil

• Autor(a) principal: Lima, Elaine Cristina Bomfim de
• Data de Publicação: 2017
• Outros Autores: Barbosa Júnior, Walter Lins, Brito, Maria Edileuza Felinto de, Melo, Fábio Lopes de, Brandão Filho, Sinval Pinto, Medeiros, Zulma Maria de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
• Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/140697
• topic: Characterization; Leishmania (L.) infantum chagasi; Visceral leishmaniasis; HIV/AIDS; Coinfection

Visceral leishmaniasis, associated with HIV/AIDS coinfection, is becoming a more aggressive disease, complicating an accurate prognosis. A 21-year-old HIV-positive female presenting with clinical features of visceral leishmaniasis was enrolled in this study. Bone marrow cytology, Novy-MacNeal-Nicolle culture and kDNA PCR of peripheral blood were all positive. Typing methods, multilocus enzyme electrophoresis and ITS1-RFLP PCR of peripheral blood confirmed infection by Leishmania (L.) infantum chagasi . PCR has proved to be safer and more affordable than other characterization methods; ITS1-RFLP PCR can diagnose and type Leishmania spp. in both endemic and non-endemic areas, favoring the prognosis and allowing the appropriate treatment of patients.