Frequency of genomic mutations mediating resistance of Mycobacterium tuberculosis isolates to rifampicin in Northern Morocco

Detalhes bibliográficos
Autor(a) principal: Karimi, Hind
Data de Publicação: 2020
Outros Autores: Oudghiri, Amal, En-nanei, Latifa, Mzibri, Mohammed El, Laglaoui, Amin, Chaoui, Imane, Abid, Mohammed
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/172368
Resumo: Drug resistant tuberculosis (DR-TB) is challenging particularly in developing countries. As such, a previous investigation gave the first insight into the mutational status of the Rifampicin Resistance Determining Region (RRDR) of rpoB gene among a restricted number of MTB patients’ residents in the Northern Morocco. The purpose of this study was to investigate rpoB mutation types and frequencies associated with resistance to Rifampicin in a larger panel of MTB patients and to evaluate the usefulness of these mutations to improve the diagnosis of resistance to Rifampicin. A panel of 301 consecutive sputum samples belonging to patients suscpected of having TB from Northern Morocco was collected at the Pasteur Institute of Tangier between 2014-2017. Samples were subjected to conventionel microbiological tests. Evaluation of rpoB muational status was assessed by PCR amplification and sequencing of the RRDR of the rpoB gene. DST results showed that 26.4% of strains were MDR. Sequencing results reported single point mutations in 36 of 65 RIFR isolates of which two had two mutations. Aminoacid substitutions in the codon Ser531Leu occurred at the highest frequency (34.46%). Overall, 10 aminoacid substitutions have been registered, and the H526S substitution was reported for the first time. The present study highlighted that resistance to RIF is a reliable marker of MDR-TB, the common mutations successfully detected in the rpoB 531, rpoB526 and rpoB516 codons provide a foundation for the implementation of molecular approaches such as Hain and GeneXpert as a routine tests to detect DR-TB. However, considerable work is still necessary to identify extensive mutations associated with DR-TB.
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spelling Frequency of genomic mutations mediating resistance of Mycobacterium tuberculosis isolates to rifampicin in Northern MoroccoMycobacterium tuberculosisTuberculosisMDR-TBXDR-TBDNA sequencingDrug susceptibility testingResistance to RifampicinrpoB mutationsMoroccoDrug resistant tuberculosis (DR-TB) is challenging particularly in developing countries. As such, a previous investigation gave the first insight into the mutational status of the Rifampicin Resistance Determining Region (RRDR) of rpoB gene among a restricted number of MTB patients’ residents in the Northern Morocco. The purpose of this study was to investigate rpoB mutation types and frequencies associated with resistance to Rifampicin in a larger panel of MTB patients and to evaluate the usefulness of these mutations to improve the diagnosis of resistance to Rifampicin. A panel of 301 consecutive sputum samples belonging to patients suscpected of having TB from Northern Morocco was collected at the Pasteur Institute of Tangier between 2014-2017. Samples were subjected to conventionel microbiological tests. Evaluation of rpoB muational status was assessed by PCR amplification and sequencing of the RRDR of the rpoB gene. DST results showed that 26.4% of strains were MDR. Sequencing results reported single point mutations in 36 of 65 RIFR isolates of which two had two mutations. Aminoacid substitutions in the codon Ser531Leu occurred at the highest frequency (34.46%). Overall, 10 aminoacid substitutions have been registered, and the H526S substitution was reported for the first time. The present study highlighted that resistance to RIF is a reliable marker of MDR-TB, the common mutations successfully detected in the rpoB 531, rpoB526 and rpoB516 codons provide a foundation for the implementation of molecular approaches such as Hain and GeneXpert as a routine tests to detect DR-TB. However, considerable work is still necessary to identify extensive mutations associated with DR-TB.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2020-07-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/xmlhttps://www.revistas.usp.br/rimtsp/article/view/17236810.1590/s1678-9946202062037Revista do Instituto de Medicina Tropical de São Paulo; Vol. 62 (2020); e37Revista do Instituto de Medicina Tropical de São Paulo; Vol. 62 (2020); e37Revista do Instituto de Medicina Tropical de São Paulo; v. 62 (2020); e371678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/172368/161987https://www.revistas.usp.br/rimtsp/article/view/172368/161988Copyright (c) 2020 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessKarimi, HindOudghiri, AmalEn-nanei, LatifaMzibri, Mohammed ElLaglaoui, AminChaoui, ImaneAbid, Mohammed2020-10-26T17:39:25Zoai:revistas.usp.br:article/172368Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:52.662559Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Frequency of genomic mutations mediating resistance of Mycobacterium tuberculosis isolates to rifampicin in Northern Morocco
title Frequency of genomic mutations mediating resistance of Mycobacterium tuberculosis isolates to rifampicin in Northern Morocco
spellingShingle Frequency of genomic mutations mediating resistance of Mycobacterium tuberculosis isolates to rifampicin in Northern Morocco
Karimi, Hind
Mycobacterium tuberculosis
Tuberculosis
MDR-TB
XDR-TB
DNA sequencing
Drug susceptibility testing
Resistance to Rifampicin
rpoB mutations
Morocco
title_short Frequency of genomic mutations mediating resistance of Mycobacterium tuberculosis isolates to rifampicin in Northern Morocco
title_full Frequency of genomic mutations mediating resistance of Mycobacterium tuberculosis isolates to rifampicin in Northern Morocco
title_fullStr Frequency of genomic mutations mediating resistance of Mycobacterium tuberculosis isolates to rifampicin in Northern Morocco
title_full_unstemmed Frequency of genomic mutations mediating resistance of Mycobacterium tuberculosis isolates to rifampicin in Northern Morocco
title_sort Frequency of genomic mutations mediating resistance of Mycobacterium tuberculosis isolates to rifampicin in Northern Morocco
author Karimi, Hind
author_facet Karimi, Hind
Oudghiri, Amal
En-nanei, Latifa
Mzibri, Mohammed El
Laglaoui, Amin
Chaoui, Imane
Abid, Mohammed
author_role author
author2 Oudghiri, Amal
En-nanei, Latifa
Mzibri, Mohammed El
Laglaoui, Amin
Chaoui, Imane
Abid, Mohammed
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Karimi, Hind
Oudghiri, Amal
En-nanei, Latifa
Mzibri, Mohammed El
Laglaoui, Amin
Chaoui, Imane
Abid, Mohammed
dc.subject.por.fl_str_mv Mycobacterium tuberculosis
Tuberculosis
MDR-TB
XDR-TB
DNA sequencing
Drug susceptibility testing
Resistance to Rifampicin
rpoB mutations
Morocco
topic Mycobacterium tuberculosis
Tuberculosis
MDR-TB
XDR-TB
DNA sequencing
Drug susceptibility testing
Resistance to Rifampicin
rpoB mutations
Morocco
description Drug resistant tuberculosis (DR-TB) is challenging particularly in developing countries. As such, a previous investigation gave the first insight into the mutational status of the Rifampicin Resistance Determining Region (RRDR) of rpoB gene among a restricted number of MTB patients’ residents in the Northern Morocco. The purpose of this study was to investigate rpoB mutation types and frequencies associated with resistance to Rifampicin in a larger panel of MTB patients and to evaluate the usefulness of these mutations to improve the diagnosis of resistance to Rifampicin. A panel of 301 consecutive sputum samples belonging to patients suscpected of having TB from Northern Morocco was collected at the Pasteur Institute of Tangier between 2014-2017. Samples were subjected to conventionel microbiological tests. Evaluation of rpoB muational status was assessed by PCR amplification and sequencing of the RRDR of the rpoB gene. DST results showed that 26.4% of strains were MDR. Sequencing results reported single point mutations in 36 of 65 RIFR isolates of which two had two mutations. Aminoacid substitutions in the codon Ser531Leu occurred at the highest frequency (34.46%). Overall, 10 aminoacid substitutions have been registered, and the H526S substitution was reported for the first time. The present study highlighted that resistance to RIF is a reliable marker of MDR-TB, the common mutations successfully detected in the rpoB 531, rpoB526 and rpoB516 codons provide a foundation for the implementation of molecular approaches such as Hain and GeneXpert as a routine tests to detect DR-TB. However, considerable work is still necessary to identify extensive mutations associated with DR-TB.
publishDate 2020
dc.date.none.fl_str_mv 2020-07-13
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/172368
10.1590/s1678-9946202062037
url https://www.revistas.usp.br/rimtsp/article/view/172368
identifier_str_mv 10.1590/s1678-9946202062037
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/172368/161987
https://www.revistas.usp.br/rimtsp/article/view/172368/161988
dc.rights.driver.fl_str_mv Copyright (c) 2020 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/xml
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 62 (2020); e37
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 62 (2020); e37
Revista do Instituto de Medicina Tropical de São Paulo; v. 62 (2020); e37
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
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