Caracterização e otimização do líquido vesicular de Echinococcus granulosus bovino para utilização no imunodiagnóstico da hidatidose por ELISA
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/30452 |
Resumo: | The aim of this work was to assess the influence in the diagnostic value for human hydatid disease of the composition of bovine hydatid cyst fluid (BHCF) obtained from fertile (FC) and non-fertile cysts (NFC). Eight batches from FC and 5 from NFC were prepared and analysed with respect to chemical composition: total protein, host-derived protein, carbohydrate and lipid contents. No differences were observed in the first two parameters but carbohydrate and lipid contents were shown to be higher in batches from FC than in those from NFC. Bands of 38 and 116 kD in SDS-PAGE profiles were observed to be present in BHCF from FC only. Two pools were prepared from BHCF batches obtained from FC (PFC) and NFC (PNFC), respectively. Antigen recognition patterns were analysed by immunoblot. Physicochemical conditions for adsorption of antigens to the polystyrene surface (ELISA plates) were optimized. The diagnostic value of both types of BHCF as well as the diagnostic relevance of oxidation of their carbohydrate moieties with periodate were assessed by ELISA using 42 serum samples from hydatid patients, 41 from patients with other disorders, and 15 from healthy donors. Reactivity of all sera against native antigen were tested with and without free phosphorylcholine. The best diagnostic efficiency was observed using BHCF from periodate-treated PFC using glycine buffer with strong ionic strength to coat ELISA plates. |
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Caracterização e otimização do líquido vesicular de Echinococcus granulosus bovino para utilização no imunodiagnóstico da hidatidose por ELISA Characterization and optimization of bovine Echinococcus granulosus cyst fluid to be used in immunodiagnosis of hydatid disease by ELISA Echinococcus granuloBovine hydatid cyst flHydatidoImmunodiagno The aim of this work was to assess the influence in the diagnostic value for human hydatid disease of the composition of bovine hydatid cyst fluid (BHCF) obtained from fertile (FC) and non-fertile cysts (NFC). Eight batches from FC and 5 from NFC were prepared and analysed with respect to chemical composition: total protein, host-derived protein, carbohydrate and lipid contents. No differences were observed in the first two parameters but carbohydrate and lipid contents were shown to be higher in batches from FC than in those from NFC. Bands of 38 and 116 kD in SDS-PAGE profiles were observed to be present in BHCF from FC only. Two pools were prepared from BHCF batches obtained from FC (PFC) and NFC (PNFC), respectively. Antigen recognition patterns were analysed by immunoblot. Physicochemical conditions for adsorption of antigens to the polystyrene surface (ELISA plates) were optimized. The diagnostic value of both types of BHCF as well as the diagnostic relevance of oxidation of their carbohydrate moieties with periodate were assessed by ELISA using 42 serum samples from hydatid patients, 41 from patients with other disorders, and 15 from healthy donors. Reactivity of all sera against native antigen were tested with and without free phosphorylcholine. The best diagnostic efficiency was observed using BHCF from periodate-treated PFC using glycine buffer with strong ionic strength to coat ELISA plates. O objetivo do presente trabalho foi testar a composição química do líquido hidático bovino (BHCF) obtido de cistos hidáticos férteis (FC) e não férteis (NFC). Oito lotes de FC e 5 de NFC foram preparados e testados quanto à composição química, proteínas totais, proteínas derivadas do hospedeiro, conteúdo de carbohidratos e lipídeos. Não foram observadas diferenças entre os dois primeiros parâmetros sendo que o conteúdo de carbohidratos e lipídeos foi maior nos lotes FC do que nos NFC. Por SDS-PAGE foram observadas bandas de 38 e 116 kD somente nos BHCF do FC. Foram preparados dois «pools» de BHCF, um de FC (PFC) e outro de NFC (PNFC). Os padrões de reconhecimento dos antígenos foram analisados por imunoblot. As condições físico-químicas para adsorção dos antígenos na superfície das placas de poliestireno (ELISA plates) foram otimizadas. O valor de diagnóstico de ambos tipos de BHCF bem como a importância diagnóstica da oxidação das moléculas de carbohidratos com periodato foram analisadas por ELISA usando 42 amostras de soro de pacientes com hidatidose, 41 de pacientes com outras doenças e 15 de doadores aparentemente saudáveis. A reatividade de todos soros contra antígenos nativos foi analisada com e sem fosforilcolina livre. A melhor eficiência diagnóstica foi observada usando BHCF de PFC tratado com periodato usando tampão glicina com forte força iônica para sensibilizar as placas de ELISA. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2000-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30452Revista do Instituto de Medicina Tropical de São Paulo; Vol. 42 No. 5 (2000); 255-262 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 42 Núm. 5 (2000); 255-262 Revista do Instituto de Medicina Tropical de São Paulo; v. 42 n. 5 (2000); 255-262 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30452/32336Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessIRABUENA, OscarNIETO, AlbertoFERREIRA, Ana MaríaBATTISTONI, JulioFERRAGUT, Gabriela2012-07-07T09:36:09Zoai:revistas.usp.br:article/30452Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:13.525604Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Caracterização e otimização do líquido vesicular de Echinococcus granulosus bovino para utilização no imunodiagnóstico da hidatidose por ELISA Characterization and optimization of bovine Echinococcus granulosus cyst fluid to be used in immunodiagnosis of hydatid disease by ELISA |
title |
Caracterização e otimização do líquido vesicular de Echinococcus granulosus bovino para utilização no imunodiagnóstico da hidatidose por ELISA |
spellingShingle |
Caracterização e otimização do líquido vesicular de Echinococcus granulosus bovino para utilização no imunodiagnóstico da hidatidose por ELISA IRABUENA, Oscar Echinococcus granulo Bovine hydatid cyst fl Hydatido Immunodiagno |
title_short |
Caracterização e otimização do líquido vesicular de Echinococcus granulosus bovino para utilização no imunodiagnóstico da hidatidose por ELISA |
title_full |
Caracterização e otimização do líquido vesicular de Echinococcus granulosus bovino para utilização no imunodiagnóstico da hidatidose por ELISA |
title_fullStr |
Caracterização e otimização do líquido vesicular de Echinococcus granulosus bovino para utilização no imunodiagnóstico da hidatidose por ELISA |
title_full_unstemmed |
Caracterização e otimização do líquido vesicular de Echinococcus granulosus bovino para utilização no imunodiagnóstico da hidatidose por ELISA |
title_sort |
Caracterização e otimização do líquido vesicular de Echinococcus granulosus bovino para utilização no imunodiagnóstico da hidatidose por ELISA |
author |
IRABUENA, Oscar |
author_facet |
IRABUENA, Oscar NIETO, Alberto FERREIRA, Ana María BATTISTONI, Julio FERRAGUT, Gabriela |
author_role |
author |
author2 |
NIETO, Alberto FERREIRA, Ana María BATTISTONI, Julio FERRAGUT, Gabriela |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
IRABUENA, Oscar NIETO, Alberto FERREIRA, Ana María BATTISTONI, Julio FERRAGUT, Gabriela |
dc.subject.por.fl_str_mv |
Echinococcus granulo Bovine hydatid cyst fl Hydatido Immunodiagno |
topic |
Echinococcus granulo Bovine hydatid cyst fl Hydatido Immunodiagno |
description |
The aim of this work was to assess the influence in the diagnostic value for human hydatid disease of the composition of bovine hydatid cyst fluid (BHCF) obtained from fertile (FC) and non-fertile cysts (NFC). Eight batches from FC and 5 from NFC were prepared and analysed with respect to chemical composition: total protein, host-derived protein, carbohydrate and lipid contents. No differences were observed in the first two parameters but carbohydrate and lipid contents were shown to be higher in batches from FC than in those from NFC. Bands of 38 and 116 kD in SDS-PAGE profiles were observed to be present in BHCF from FC only. Two pools were prepared from BHCF batches obtained from FC (PFC) and NFC (PNFC), respectively. Antigen recognition patterns were analysed by immunoblot. Physicochemical conditions for adsorption of antigens to the polystyrene surface (ELISA plates) were optimized. The diagnostic value of both types of BHCF as well as the diagnostic relevance of oxidation of their carbohydrate moieties with periodate were assessed by ELISA using 42 serum samples from hydatid patients, 41 from patients with other disorders, and 15 from healthy donors. Reactivity of all sera against native antigen were tested with and without free phosphorylcholine. The best diagnostic efficiency was observed using BHCF from periodate-treated PFC using glycine buffer with strong ionic strength to coat ELISA plates. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30452 |
url |
https://www.revistas.usp.br/rimtsp/article/view/30452 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30452/32336 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 42 No. 5 (2000); 255-262 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 42 Núm. 5 (2000); 255-262 Revista do Instituto de Medicina Tropical de São Paulo; v. 42 n. 5 (2000); 255-262 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951642818674688 |