Sharing of antigens between Plasmodium falciparum and Anopheles albimanus

Detalhes bibliográficos
Autor(a) principal: Wide, Albina
Data de Publicação: 2006
Outros Autores: Capaldo, Jacinta, Zerpa, Noraida, Pabon, Rosalba, Noda, Alfredo, Noya, Belkis, González, Julio, Noya, Oscar
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/31038
Resumo: The presence of common antigens between Plasmodium falciparum and Anopheles albimanus was demonstrated. Different groups of rabbits were immunized with: crude extract from female An. albimanus (EAaF), red blood cells infected with Plasmodium falciparum (EPfs), and the SPf66 synthetic malaria vaccine. The rabbit's polyclonal antibodies were evaluated by ELISA, Multiple Antigen Blot Assay (MABA), and immunoblotting. All extracts were immunogenic in rabbits according to these three techniques, when they were evaluated against the homologous antigens. Ten molecules were identified in female mosquitoes and also in P. falciparum antigens by the autologous sera. The electrophoretic pattern by SDS-PAGE was different for the three antigens evaluated. Cross-reactions between An. albimanus and P. falciparum were found by ELISA, MABA, and immunoblotting. Anti-P. falciparum and anti-SPf66 antibodies recognized ten and five components in the EAaF crude extract, respectively. Likewise, immune sera against female An. albimanus identified four molecules in the P. falciparum extract antigen. As far as we know, this is the first work that demonstrates shared antigens between anophelines and malaria parasites. This finding could be useful for diagnosis, vaccines, and the study of physiology of the immune response to malaria.
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spelling Sharing of antigens between Plasmodium falciparum and Anopheles albimanus Antígenos compartidos entre Plasmodium falciparum y Anopheles albimanus Anopheles albimanusPlasmodium falciparumShared antigensCross-reactionMultiple Antigen Blot Assay (MABA) The presence of common antigens between Plasmodium falciparum and Anopheles albimanus was demonstrated. Different groups of rabbits were immunized with: crude extract from female An. albimanus (EAaF), red blood cells infected with Plasmodium falciparum (EPfs), and the SPf66 synthetic malaria vaccine. The rabbit's polyclonal antibodies were evaluated by ELISA, Multiple Antigen Blot Assay (MABA), and immunoblotting. All extracts were immunogenic in rabbits according to these three techniques, when they were evaluated against the homologous antigens. Ten molecules were identified in female mosquitoes and also in P. falciparum antigens by the autologous sera. The electrophoretic pattern by SDS-PAGE was different for the three antigens evaluated. Cross-reactions between An. albimanus and P. falciparum were found by ELISA, MABA, and immunoblotting. Anti-P. falciparum and anti-SPf66 antibodies recognized ten and five components in the EAaF crude extract, respectively. Likewise, immune sera against female An. albimanus identified four molecules in the P. falciparum extract antigen. As far as we know, this is the first work that demonstrates shared antigens between anophelines and malaria parasites. This finding could be useful for diagnosis, vaccines, and the study of physiology of the immune response to malaria. Epítopes de antígenos compartidos entre Plasmodium falciparum y Anopheles albimanus fueron identificados. Diferentes grupos de conejos fueron inmunizados con: extracto crudo de mosquito hembra de An. albimanus (EAaH), glóbulos rojos infectados con P. falciparum (EPfs) y la vacuna antimalárica sintética SPf66. Los anticuerpos policlonales producidos en conejos fueron evaluados por ELISA, inmunoensayo simultáneo de múltiples antígenos (MABA) e Immunoblotting. Todos los extractos resultaron inmunogénicos cuando se evaluaron por ELISA, MABA e Immunoblotting. Diez moléculas fueron identificadas en los mosquitos hembras y diez en los antígenos de P. falciparum por los sueros autólogos. El patrón electroforético por SDS-EGPA fue diferente para los tres antígenos evaluados. La reactividad cruzada de moléculas entre An. albimanus y P. falciparum fue demostrada por ELISA, MABA e Immunoblotting. Anticuerpos anti-P. falciparum y anti-SPf66 reconocieron diez y cinco componentes respectivamente en el extracto crudo de anofelinos (EAaH). Asimismo, sueros inmunes contra An. albimanus hembra identificaron cuatro moléculas en el extracto del antígeno de P. falciparum. Hasta el presente, este es el primer estudio en el que se demuestra la presencia de antígenos compartidos entre anofelinos y los parásitos de malaria. Este hallazgo podría ser de relevancia para el diagnóstico, vacunas e interpretación de la fisiopatología de la respuesta inmunitaria en malaria. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2006-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/31038Revista do Instituto de Medicina Tropical de São Paulo; Vol. 48 No. 6 (2006); 327-332 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 48 Núm. 6 (2006); 327-332 Revista do Instituto de Medicina Tropical de São Paulo; v. 48 n. 6 (2006); 327-332 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/31038/32922Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessWide, AlbinaCapaldo, JacintaZerpa, NoraidaPabon, RosalbaNoda, AlfredoNoya, BelkisGonzález, JulioNoya, Oscar2012-07-07T18:56:25Zoai:revistas.usp.br:article/31038Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:44.667550Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Sharing of antigens between Plasmodium falciparum and Anopheles albimanus
Antígenos compartidos entre Plasmodium falciparum y Anopheles albimanus
title Sharing of antigens between Plasmodium falciparum and Anopheles albimanus
spellingShingle Sharing of antigens between Plasmodium falciparum and Anopheles albimanus
Wide, Albina
Anopheles albimanus
Plasmodium falciparum
Shared antigens
Cross-reaction
Multiple Antigen Blot Assay (MABA)
title_short Sharing of antigens between Plasmodium falciparum and Anopheles albimanus
title_full Sharing of antigens between Plasmodium falciparum and Anopheles albimanus
title_fullStr Sharing of antigens between Plasmodium falciparum and Anopheles albimanus
title_full_unstemmed Sharing of antigens between Plasmodium falciparum and Anopheles albimanus
title_sort Sharing of antigens between Plasmodium falciparum and Anopheles albimanus
author Wide, Albina
author_facet Wide, Albina
Capaldo, Jacinta
Zerpa, Noraida
Pabon, Rosalba
Noda, Alfredo
Noya, Belkis
González, Julio
Noya, Oscar
author_role author
author2 Capaldo, Jacinta
Zerpa, Noraida
Pabon, Rosalba
Noda, Alfredo
Noya, Belkis
González, Julio
Noya, Oscar
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Wide, Albina
Capaldo, Jacinta
Zerpa, Noraida
Pabon, Rosalba
Noda, Alfredo
Noya, Belkis
González, Julio
Noya, Oscar
dc.subject.por.fl_str_mv Anopheles albimanus
Plasmodium falciparum
Shared antigens
Cross-reaction
Multiple Antigen Blot Assay (MABA)
topic Anopheles albimanus
Plasmodium falciparum
Shared antigens
Cross-reaction
Multiple Antigen Blot Assay (MABA)
description The presence of common antigens between Plasmodium falciparum and Anopheles albimanus was demonstrated. Different groups of rabbits were immunized with: crude extract from female An. albimanus (EAaF), red blood cells infected with Plasmodium falciparum (EPfs), and the SPf66 synthetic malaria vaccine. The rabbit's polyclonal antibodies were evaluated by ELISA, Multiple Antigen Blot Assay (MABA), and immunoblotting. All extracts were immunogenic in rabbits according to these three techniques, when they were evaluated against the homologous antigens. Ten molecules were identified in female mosquitoes and also in P. falciparum antigens by the autologous sera. The electrophoretic pattern by SDS-PAGE was different for the three antigens evaluated. Cross-reactions between An. albimanus and P. falciparum were found by ELISA, MABA, and immunoblotting. Anti-P. falciparum and anti-SPf66 antibodies recognized ten and five components in the EAaF crude extract, respectively. Likewise, immune sera against female An. albimanus identified four molecules in the P. falciparum extract antigen. As far as we know, this is the first work that demonstrates shared antigens between anophelines and malaria parasites. This finding could be useful for diagnosis, vaccines, and the study of physiology of the immune response to malaria.
publishDate 2006
dc.date.none.fl_str_mv 2006-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/31038
url https://www.revistas.usp.br/rimtsp/article/view/31038
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/31038/32922
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 48 No. 6 (2006); 327-332
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 48 Núm. 6 (2006); 327-332
Revista do Instituto de Medicina Tropical de São Paulo; v. 48 n. 6 (2006); 327-332
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
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reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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