A classificação de Bethesda: uma análise morfométrica das biópsias do colo uterino

• Autor(a) principal: Miranda, Carlos Roberto de Resende
• Data de Publicação: 2022
• Outros Autores: Deligdisch, Liane, Gil, Joan, Dottino, Peter Remson, Cohen, Carmel Jonathan
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista Brasileira de Cancerologia (Online)
• Texto Completo: https://rbc.inca.gov.br/index.php/revista/article/view/2954
• topic: Infecções por Papillomavirus; Lesões Intraepiteliais Escamosas; Displasia do Colo do Útero; Papillomavirus Infections; Squamous Intraepithelial Lesions; Uterine Cervical Dysplasia; Infecciones por Papillomavirus; Lesiones Intraepiteliales Escamosas; Displasia del Cuello del Útero

Because of the possibility of extending the Bethesda System to histologic reports, we consider in this morphometric study some of main issues involved in the HPV-related lesions, in particular the possibility of establishing a cut of between low and high-grade lesions. We compared histological and cytological features of cervical precancerous conditions by morphometric analysis. Histologic sections from 62 colposcopically guided biopsies of the cervix were studied by computerized morphometry. The cases had been grouped according to the subjective dia gnosis into three groups: normal (33 cases), Squamous Intraepithelial Lesions - Low Grade (SIL-LG, 21 cases), and Squamous Intra epithelial Lesions - High Grade (SIL-HG, 8 cases). We carried out our study using programs called DRAW and COUNT3, that considers the following parameters: area, length, maximum chord, roundness, of the nuclei of cells in the lesion, and celullarity, from the outer Iayer of cervical epithelium (superficial). After measuremens of the cells of interest, we analyzed the results by multivariate analysis and computed posterior probabilities to confirm the homogeneity of the cases in the dia gnostic groups. Area and celiularity seem to be the most reliable parameters. The posterior probability of all but six cases were higher than 0.975. Morphometric procedures fuliy support the distinction among normal squamous tissue, SIL-LG and SIL-HG.