The Inflammatory Mosaic in the Histological Subtypes of Basal Cell Carcinoma
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista Brasileira de Cancerologia (Online) |
Texto Completo: | https://rbc.inca.gov.br/index.php/revista/article/view/971 |
Resumo: | Introduction: Basal Cell Carcinoma (BCC) is defined as a slow-growing locally invasive tumor. As it spreads, it elicits a chronic inflammatory process with the recruitment of several cell types. Objective: To characterize the inflammatory infiltrate describing the different subtypes of BCC through the identification and quantification of its cells. Method: This was a retrospective study of 71 paraffin blocks from patients diagnosed with non-recurrent BCC. The immunohistochemical analyses were performed using the Streptavidin-biotin-peroxidase technique (CD3, CD20, CD68, CD8, CD4, and Ki-67 cell MAST), and the toluidine blue technique for mast cells. Results: The most frequent subtypes found were infiltrating (26%) and superficial (23%) BCC. Regarding the composition of the inflammatory infiltrate, the TCD 4 + lymphocytes corresponded to the largest population (216.2 ± 22.23), followed by mast cells (111.0 ± 7.88), TCD8 + lymphocytes (57.38 ± 5.94), B lymphocytes (55.9±6.83) and macrophages (21.18 ± 2.58). The total cell proliferative activity was generally low (47.61 ± 7.48), except for more aggressive forms of the disease, in which infiltrates rich in mast cells could be found. The adenoid subtype showed a denser infiltrate, while the Cystic subtype presented a scanty infiltrate. There was an inverse relationship between the number of mast cells and the number of T lymphocytes, without correlation with aggressiveness. Conclusion: In the BCC, the peritumoral inflammatory infiltrates suggests an immune response mediated by TCD 4+ and a composition which varies according to the type of tumor. It has been suggested that the characteristics of each tumor type might reveal differences in the tumor tissue microenvironment, which cause alterations in the composition of the infiltrate, thereby favoring or impeding tumor growth. |
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The Inflammatory Mosaic in the Histological Subtypes of Basal Cell CarcinomaMosaico Inflamatorio en los Subtipos de Carcinoma de Células BasalesO Mosaico Inflamatório nos Subtipos de Carcinoma BasocelularHumanosNeoplasias CutâneasCarcinoma Basocelular-fisiopatologiaCarcinoma Basocelular-patologiaMastócitosHumansSkin NeoplasmsCarcinoma, Basal Cell-physiopathologyCarcinoma, Basal Cell-pathologyMast CellsHumanosNeoplasias CutâneasCarcinoma Basocelular-fisiopatologíaCarcinoma BasocelularfisiopatologíaMastocítosIntroduction: Basal Cell Carcinoma (BCC) is defined as a slow-growing locally invasive tumor. As it spreads, it elicits a chronic inflammatory process with the recruitment of several cell types. Objective: To characterize the inflammatory infiltrate describing the different subtypes of BCC through the identification and quantification of its cells. Method: This was a retrospective study of 71 paraffin blocks from patients diagnosed with non-recurrent BCC. The immunohistochemical analyses were performed using the Streptavidin-biotin-peroxidase technique (CD3, CD20, CD68, CD8, CD4, and Ki-67 cell MAST), and the toluidine blue technique for mast cells. Results: The most frequent subtypes found were infiltrating (26%) and superficial (23%) BCC. Regarding the composition of the inflammatory infiltrate, the TCD 4 + lymphocytes corresponded to the largest population (216.2 ± 22.23), followed by mast cells (111.0 ± 7.88), TCD8 + lymphocytes (57.38 ± 5.94), B lymphocytes (55.9±6.83) and macrophages (21.18 ± 2.58). The total cell proliferative activity was generally low (47.61 ± 7.48), except for more aggressive forms of the disease, in which infiltrates rich in mast cells could be found. The adenoid subtype showed a denser infiltrate, while the Cystic subtype presented a scanty infiltrate. There was an inverse relationship between the number of mast cells and the number of T lymphocytes, without correlation with aggressiveness. Conclusion: In the BCC, the peritumoral inflammatory infiltrates suggests an immune response mediated by TCD 4+ and a composition which varies according to the type of tumor. It has been suggested that the characteristics of each tumor type might reveal differences in the tumor tissue microenvironment, which cause alterations in the composition of the infiltrate, thereby favoring or impeding tumor growth. Introducción: El carcinoma de celulas basales (CCB) se define como un tumor localmente invasivo y de lenta progresion. A medida que se propaga, se inicia un proceso inflamatorio cronico con la incorporacion de distintos tipos de celulas. Objetivo: Caracterizar el infiltrado inflamatorio en los diferentes subtipos de CCB, a traves de la identificacion y cuantificacion de sus celulas. Método: Se trato de un estudio retrospectivo de 71 bloques de parafina de pacientes diagnosticados con CCB no recurrentes. El analisis inmune-histoquimicas se llevaron a cabo por la tecnica de Estreptavidina-biotina-peroxidasa (CD3, CD20, CD68, CD8, CD4, Ki-67 y mastocitos), ademas de la tecnica de azul de toluidina para mastocitos. Resultados: Los subtipos mas frecuentes fueron el infiltrado (26%) y el superficial (23%). En la composicion del infiltrado inflamatorio, los linfocitos TCD 4+ correspondian a la poblacion mas numerosa (216,2 ± 22,23), seguida de mastocitos (111,0 ± 7,88), los linfocitos TCD 8+ (57,38 ± 5,94), los linfocitos B (55,9 ± 6,83) y macrofagos (21,18 ± 2,58). Hubo una baja actividad proliferativa celular total (47,61 ± 7,48), sin embargo en formas mas agresivas este dato fue lo opuesto y con infiltrado rico en mastocitos. El subtipo adenoidea presento el mas denso infiltrado, mientras que el quistico presento el mas discreto. Hemos observado una relacion inversa entre el numero de mastocitos y de linfocitos T, sin correlacion con la agresividad. Conclusión: En el CCB, el infiltrado inflamatorio peritumoral sugiere una respuesta inmunologica mediada por celulas TCD 4+ y composicion puede variar segun el tipo de tumor. Se sugiere que las caracteristicas de cada tumor puedan promover las diferencias en el microambiente del tejido, induciendo cambios en la composicion de la infiltracion que podria tanto ayudar, como impedir el crecimiento del tumor.Introdução: O carcinoma basocelular (CBC) e definido como um tumor localmente invasivo e de progressão lenta. A medida que ele se espalha, inicia-se um processo inflamatório crônico com recrutamento de diversos tipos celulares. Objetivo: Caracterizar o infiltrado inflamatório, nos diferentes subtipos de CBC, através da identificação e quantificação de suas células. Método: Foi realizado estudo retrospectivo de 71 blocos de parafina de pacientes diagnosticados com CBC não recorrente. As analises imuno-histoquímicas foram realizadas pela técnica de estreptoavidina-biotinaperoxidase (CD3, CD20, CD68, CD8, CD4, Ki-67 e MAST cell), além da técnica do azul de toluidina para mastócitos. Resultados: Os subtipos mais frequentes foram o infiltrativo (26%) e o superficial (23%). Na composição do infiltrado inflamatório, os linfócitos TCD 4+ corresponderam a população mais numerosa (216,2 ± 22,23), seguida por mastócitos (111,0 ± 7,88), linfócitos TCD 8+ (57,38 ± 5,94), linfócitos B (55,9 ± 6,83) e macrófagos (21,18 ± 2,58). Houve uma baixa atividade proliferativa celular total (47,61 ± 7,48), no entanto em formas mais agressivas esse dado foi inverso e com infiltrado rico em mastócitos. O subtipo adenoide apresentou o mais denso infiltrado, enquanto o cístico apresentou o mais discreto. Observou-se relação inversa entre o número de mastócitos e de linfócitos T, sem correlação com agressividade. Conclusão: No CBC, o infiltrado inflamatório peritumoral sugere uma resposta imunológica mediada por células TCD 4+ e composição variando de acordo com o tipo de tumor. Sugere-se que as características de cada tumor possam promover diferenças no microambiente tecidual, induzindo alterações na composição do infiltrado que poderiam tanto auxiliar quanto impedir o crescimento tumoral. INCA2013-12-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtigos, Avaliado pelos paresapplication/pdfhttps://rbc.inca.gov.br/index.php/revista/article/view/97110.32635/2176-9745.RBC.2013v59n4.971Revista Brasileira de Cancerologia; Vol. 59 No. 4 (2013): Oct./Nov./Dec.; 531-538Revista Brasileira de Cancerologia; Vol. 59 Núm. 4 (2013): oct./nov./dic.; 531-538Revista Brasileira de Cancerologia; v. 59 n. 4 (2013): out./nov./dez.; 531-5382176-9745reponame:Revista Brasileira de Cancerologia (Online)instname:Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA)instacron:INCAporhttps://rbc.inca.gov.br/index.php/revista/article/view/971/585Ramos Machado Braga, JacquelineSadigursky, Moysésde Almeida Barbosa Junior, Aryoninfo:eu-repo/semantics/openAccess2021-11-29T20:10:49Zoai:rbc.inca.gov.br:article/971Revistahttps://rbc.inca.gov.br/index.php/revistaPUBhttps://rbc.inca.gov.br/index.php/revista/oairbc@inca.gov.br0034-71162176-9745opendoar:2021-11-29T20:10:49Revista Brasileira de Cancerologia (Online) - Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA)false |
dc.title.none.fl_str_mv |
The Inflammatory Mosaic in the Histological Subtypes of Basal Cell Carcinoma Mosaico Inflamatorio en los Subtipos de Carcinoma de Células Basales O Mosaico Inflamatório nos Subtipos de Carcinoma Basocelular |
title |
The Inflammatory Mosaic in the Histological Subtypes of Basal Cell Carcinoma |
spellingShingle |
The Inflammatory Mosaic in the Histological Subtypes of Basal Cell Carcinoma Ramos Machado Braga, Jacqueline Humanos Neoplasias Cutâneas Carcinoma Basocelular-fisiopatologia Carcinoma Basocelular-patologia Mastócitos Humans Skin Neoplasms Carcinoma, Basal Cell-physiopathology Carcinoma, Basal Cell-pathology Mast Cells Humanos Neoplasias Cutâneas Carcinoma Basocelular-fisiopatología Carcinoma Basocelularfisiopatología Mastocítos |
title_short |
The Inflammatory Mosaic in the Histological Subtypes of Basal Cell Carcinoma |
title_full |
The Inflammatory Mosaic in the Histological Subtypes of Basal Cell Carcinoma |
title_fullStr |
The Inflammatory Mosaic in the Histological Subtypes of Basal Cell Carcinoma |
title_full_unstemmed |
The Inflammatory Mosaic in the Histological Subtypes of Basal Cell Carcinoma |
title_sort |
The Inflammatory Mosaic in the Histological Subtypes of Basal Cell Carcinoma |
author |
Ramos Machado Braga, Jacqueline |
author_facet |
Ramos Machado Braga, Jacqueline Sadigursky, Moysés de Almeida Barbosa Junior, Aryon |
author_role |
author |
author2 |
Sadigursky, Moysés de Almeida Barbosa Junior, Aryon |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Ramos Machado Braga, Jacqueline Sadigursky, Moysés de Almeida Barbosa Junior, Aryon |
dc.subject.por.fl_str_mv |
Humanos Neoplasias Cutâneas Carcinoma Basocelular-fisiopatologia Carcinoma Basocelular-patologia Mastócitos Humans Skin Neoplasms Carcinoma, Basal Cell-physiopathology Carcinoma, Basal Cell-pathology Mast Cells Humanos Neoplasias Cutâneas Carcinoma Basocelular-fisiopatología Carcinoma Basocelularfisiopatología Mastocítos |
topic |
Humanos Neoplasias Cutâneas Carcinoma Basocelular-fisiopatologia Carcinoma Basocelular-patologia Mastócitos Humans Skin Neoplasms Carcinoma, Basal Cell-physiopathology Carcinoma, Basal Cell-pathology Mast Cells Humanos Neoplasias Cutâneas Carcinoma Basocelular-fisiopatología Carcinoma Basocelularfisiopatología Mastocítos |
description |
Introduction: Basal Cell Carcinoma (BCC) is defined as a slow-growing locally invasive tumor. As it spreads, it elicits a chronic inflammatory process with the recruitment of several cell types. Objective: To characterize the inflammatory infiltrate describing the different subtypes of BCC through the identification and quantification of its cells. Method: This was a retrospective study of 71 paraffin blocks from patients diagnosed with non-recurrent BCC. The immunohistochemical analyses were performed using the Streptavidin-biotin-peroxidase technique (CD3, CD20, CD68, CD8, CD4, and Ki-67 cell MAST), and the toluidine blue technique for mast cells. Results: The most frequent subtypes found were infiltrating (26%) and superficial (23%) BCC. Regarding the composition of the inflammatory infiltrate, the TCD 4 + lymphocytes corresponded to the largest population (216.2 ± 22.23), followed by mast cells (111.0 ± 7.88), TCD8 + lymphocytes (57.38 ± 5.94), B lymphocytes (55.9±6.83) and macrophages (21.18 ± 2.58). The total cell proliferative activity was generally low (47.61 ± 7.48), except for more aggressive forms of the disease, in which infiltrates rich in mast cells could be found. The adenoid subtype showed a denser infiltrate, while the Cystic subtype presented a scanty infiltrate. There was an inverse relationship between the number of mast cells and the number of T lymphocytes, without correlation with aggressiveness. Conclusion: In the BCC, the peritumoral inflammatory infiltrates suggests an immune response mediated by TCD 4+ and a composition which varies according to the type of tumor. It has been suggested that the characteristics of each tumor type might reveal differences in the tumor tissue microenvironment, which cause alterations in the composition of the infiltrate, thereby favoring or impeding tumor growth. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-31 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artigos, Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rbc.inca.gov.br/index.php/revista/article/view/971 10.32635/2176-9745.RBC.2013v59n4.971 |
url |
https://rbc.inca.gov.br/index.php/revista/article/view/971 |
identifier_str_mv |
10.32635/2176-9745.RBC.2013v59n4.971 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rbc.inca.gov.br/index.php/revista/article/view/971/585 |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
INCA |
publisher.none.fl_str_mv |
INCA |
dc.source.none.fl_str_mv |
Revista Brasileira de Cancerologia; Vol. 59 No. 4 (2013): Oct./Nov./Dec.; 531-538 Revista Brasileira de Cancerologia; Vol. 59 Núm. 4 (2013): oct./nov./dic.; 531-538 Revista Brasileira de Cancerologia; v. 59 n. 4 (2013): out./nov./dez.; 531-538 2176-9745 reponame:Revista Brasileira de Cancerologia (Online) instname:Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA) instacron:INCA |
instname_str |
Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA) |
instacron_str |
INCA |
institution |
INCA |
reponame_str |
Revista Brasileira de Cancerologia (Online) |
collection |
Revista Brasileira de Cancerologia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Cancerologia (Online) - Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA) |
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rbc@inca.gov.br |
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