Tissue reparation histology of open cutaneous wonds by metotrexate: experimental studies of female rats

Detalhes bibliográficos
Autor(a) principal: Carneiro, Paulo Cesar Alves
Data de Publicação: 2022
Outros Autores: Gutman, Teresa Cristina Ferreira, Castro, Onofre Ferreira de, Rodrigues, Pedro Carvalho, Pinheiro, Juliano Moraes, Oliveira, Dilon Pinheiro de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista Brasileira de Cancerologia (Online)
Texto Completo: https://rbc.inca.gov.br/index.php/revista/article/view/2885
Resumo: The authors have studied the action of methotrexate applied intramusculary on healing of cutaneous circular open wound on female ratsfrom the macroscopic and histological point of view. 36 Wistar female rats scaling 250.0 grams on an average. On the 18 animais of the control group (Group 1) sodium chloride was injected intramuscularly into the left back member in an equivalent to the amount required for the group treated, immediately after making a skin open wound with a 6 mm diameter punch on the back of the animal at about 5 cm from the lower edges of the external ears. The same surgical procedure of the control group was carried out in the experimental group (Group 2), and methotrexate (1 mg/kg ofbody weight) was applied intramuscularly into the same anatomic location. They were killed on the 4th and 7th days after operation. The histologic alterations ofthe ejfect of methotrexate evidenced through optical microscopy, such as reduction of tissue area of granulation and necrosis (p < 0.001), the number of round nuclei (p < 0.001), in the number of fibroblasts and histiocytes in transformation (fusiform nuclei field) (p < 0.001), in the distance getween the edges of the wound (p < 0.001) and in the number of vessels/field (p < 0.05), were predominant on the 4th and 7th days. It is concluded that, the methotrexate attenuates the inflammatory answer actuating in the initial and fibroplasia phases, however, this has accelerated the reepitelization process.
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spelling Tissue reparation histology of open cutaneous wonds by metotrexate: experimental studies of female ratsAspectos histológicos da reparação tecidual por efeito do metotrexato em feridas cutâneas abertas: estudo experimental em ratasPeleCicatrização das FeridasHistologiaMetotrexatoEfeitos AdversosSkinWound HealingHistologyMethotrexateDrug EffectsThe authors have studied the action of methotrexate applied intramusculary on healing of cutaneous circular open wound on female ratsfrom the macroscopic and histological point of view. 36 Wistar female rats scaling 250.0 grams on an average. On the 18 animais of the control group (Group 1) sodium chloride was injected intramuscularly into the left back member in an equivalent to the amount required for the group treated, immediately after making a skin open wound with a 6 mm diameter punch on the back of the animal at about 5 cm from the lower edges of the external ears. The same surgical procedure of the control group was carried out in the experimental group (Group 2), and methotrexate (1 mg/kg ofbody weight) was applied intramuscularly into the same anatomic location. They were killed on the 4th and 7th days after operation. The histologic alterations ofthe ejfect of methotrexate evidenced through optical microscopy, such as reduction of tissue area of granulation and necrosis (p < 0.001), the number of round nuclei (p < 0.001), in the number of fibroblasts and histiocytes in transformation (fusiform nuclei field) (p < 0.001), in the distance getween the edges of the wound (p < 0.001) and in the number of vessels/field (p < 0.05), were predominant on the 4th and 7th days. It is concluded that, the methotrexate attenuates the inflammatory answer actuating in the initial and fibroplasia phases, however, this has accelerated the reepitelization process.Foi estudada a ação do metotrexato administrado por via intramuscular sobre a cicatrização de feridas cutâneas circulares abertas em ratas, do ponto de vista histológico. Foram operadas 36 ratas Wistar, com peso médio de 250,0 gramas. Nos 18 animais do grupo-controle (Grupo 1) foi injetado soro fisiológico 0,9% por via intramuscular, em volume equivalente ao necessário ao grupo tratado, no membro traseiro esquerdo, imediatamente após a confecção de uma ferida aberta de pele, provocada com vazador de 6 mm de diâmetro no dorso do animal, a cerca de 5 cm das bordas inferiores dos pavilhões auriculares. No grupo-experimento (Grupo 2) realizou-se o mesmo procedimento cirúrgico do grupo-controle e administrou-se metotrexato (1 mg/kg de peso corpóreo) por via intramuscular na mesma localização anatômica. Foram sacrificadas no 4“ e T dias de pós-operatório. As alterações histológicas do efeito do metotrexato, evidenciado através da microscopia óptica, se fizeram predominantemente no 7° dia de pós-operatório, tais como: a redução da área de tecido de granulação e necrose (p < 0,001), do número de núcleos redondos (p < 0,001), do número de polimorfonucleares (p < 0,001), do número de fibroblastos e histiócitos em transformação (núcleos fusiformes/campo) (p < 0,001), da distância entre as bordas da ferida (p < 0,001) e do número de vasos/campo (p < 0,005). Concluem que o metotrexato atenua a resposta inflamatória, atuando nas fases iniciais e de fibroplasia, entretanto, acelerou o processo de reepitelização.INCA2022-10-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtigos, Avaliado pelos paresapplication/pdfhttps://rbc.inca.gov.br/index.php/revista/article/view/288510.32635/2176-9745.RBC.1996v42n1.2885Revista Brasileira de Cancerologia; Vol. 42 No. 1 (1996): Jan./Feb./Mar.; 49-59Revista Brasileira de Cancerologia; Vol. 42 Núm. 1 (1996): ene./feb./mar.; 49-59Revista Brasileira de Cancerologia; v. 42 n. 1 (1996): jan./fev./mar.; 49-592176-9745reponame:Revista Brasileira de Cancerologia (Online)instname:Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA)instacron:INCAporhttps://rbc.inca.gov.br/index.php/revista/article/view/2885/1762https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCarneiro, Paulo Cesar AlvesGutman, Teresa Cristina FerreiraCastro, Onofre Ferreira deRodrigues, Pedro CarvalhoPinheiro, Juliano MoraesOliveira, Dilon Pinheiro de2023-06-19T13:18:58Zoai:rbc.inca.gov.br:article/2885Revistahttps://rbc.inca.gov.br/index.php/revistaPUBhttps://rbc.inca.gov.br/index.php/revista/oairbc@inca.gov.br0034-71162176-9745opendoar:2023-06-19T13:18:58Revista Brasileira de Cancerologia (Online) - Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA)false
dc.title.none.fl_str_mv Tissue reparation histology of open cutaneous wonds by metotrexate: experimental studies of female rats
Aspectos histológicos da reparação tecidual por efeito do metotrexato em feridas cutâneas abertas: estudo experimental em ratas
title Tissue reparation histology of open cutaneous wonds by metotrexate: experimental studies of female rats
spellingShingle Tissue reparation histology of open cutaneous wonds by metotrexate: experimental studies of female rats
Carneiro, Paulo Cesar Alves
Pele
Cicatrização das Feridas
Histologia
Metotrexato
Efeitos Adversos
Skin
Wound Healing
Histology
Methotrexate
Drug Effects
title_short Tissue reparation histology of open cutaneous wonds by metotrexate: experimental studies of female rats
title_full Tissue reparation histology of open cutaneous wonds by metotrexate: experimental studies of female rats
title_fullStr Tissue reparation histology of open cutaneous wonds by metotrexate: experimental studies of female rats
title_full_unstemmed Tissue reparation histology of open cutaneous wonds by metotrexate: experimental studies of female rats
title_sort Tissue reparation histology of open cutaneous wonds by metotrexate: experimental studies of female rats
author Carneiro, Paulo Cesar Alves
author_facet Carneiro, Paulo Cesar Alves
Gutman, Teresa Cristina Ferreira
Castro, Onofre Ferreira de
Rodrigues, Pedro Carvalho
Pinheiro, Juliano Moraes
Oliveira, Dilon Pinheiro de
author_role author
author2 Gutman, Teresa Cristina Ferreira
Castro, Onofre Ferreira de
Rodrigues, Pedro Carvalho
Pinheiro, Juliano Moraes
Oliveira, Dilon Pinheiro de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Carneiro, Paulo Cesar Alves
Gutman, Teresa Cristina Ferreira
Castro, Onofre Ferreira de
Rodrigues, Pedro Carvalho
Pinheiro, Juliano Moraes
Oliveira, Dilon Pinheiro de
dc.subject.por.fl_str_mv Pele
Cicatrização das Feridas
Histologia
Metotrexato
Efeitos Adversos
Skin
Wound Healing
Histology
Methotrexate
Drug Effects
topic Pele
Cicatrização das Feridas
Histologia
Metotrexato
Efeitos Adversos
Skin
Wound Healing
Histology
Methotrexate
Drug Effects
description The authors have studied the action of methotrexate applied intramusculary on healing of cutaneous circular open wound on female ratsfrom the macroscopic and histological point of view. 36 Wistar female rats scaling 250.0 grams on an average. On the 18 animais of the control group (Group 1) sodium chloride was injected intramuscularly into the left back member in an equivalent to the amount required for the group treated, immediately after making a skin open wound with a 6 mm diameter punch on the back of the animal at about 5 cm from the lower edges of the external ears. The same surgical procedure of the control group was carried out in the experimental group (Group 2), and methotrexate (1 mg/kg ofbody weight) was applied intramuscularly into the same anatomic location. They were killed on the 4th and 7th days after operation. The histologic alterations ofthe ejfect of methotrexate evidenced through optical microscopy, such as reduction of tissue area of granulation and necrosis (p < 0.001), the number of round nuclei (p < 0.001), in the number of fibroblasts and histiocytes in transformation (fusiform nuclei field) (p < 0.001), in the distance getween the edges of the wound (p < 0.001) and in the number of vessels/field (p < 0.05), were predominant on the 4th and 7th days. It is concluded that, the methotrexate attenuates the inflammatory answer actuating in the initial and fibroplasia phases, however, this has accelerated the reepitelization process.
publishDate 2022
dc.date.none.fl_str_mv 2022-10-04
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Artigos, Avaliado pelos pares
format article
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dc.identifier.uri.fl_str_mv https://rbc.inca.gov.br/index.php/revista/article/view/2885
10.32635/2176-9745.RBC.1996v42n1.2885
url https://rbc.inca.gov.br/index.php/revista/article/view/2885
identifier_str_mv 10.32635/2176-9745.RBC.1996v42n1.2885
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rbc.inca.gov.br/index.php/revista/article/view/2885/1762
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
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dc.publisher.none.fl_str_mv INCA
publisher.none.fl_str_mv INCA
dc.source.none.fl_str_mv Revista Brasileira de Cancerologia; Vol. 42 No. 1 (1996): Jan./Feb./Mar.; 49-59
Revista Brasileira de Cancerologia; Vol. 42 Núm. 1 (1996): ene./feb./mar.; 49-59
Revista Brasileira de Cancerologia; v. 42 n. 1 (1996): jan./fev./mar.; 49-59
2176-9745
reponame:Revista Brasileira de Cancerologia (Online)
instname:Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA)
instacron:INCA
instname_str Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA)
instacron_str INCA
institution INCA
reponame_str Revista Brasileira de Cancerologia (Online)
collection Revista Brasileira de Cancerologia (Online)
repository.name.fl_str_mv Revista Brasileira de Cancerologia (Online) - Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA)
repository.mail.fl_str_mv rbc@inca.gov.br
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