Tissue reparation histology of open cutaneous wonds by metotrexate: experimental studies of female rats
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista Brasileira de Cancerologia (Online) |
Texto Completo: | https://rbc.inca.gov.br/index.php/revista/article/view/2885 |
Resumo: | The authors have studied the action of methotrexate applied intramusculary on healing of cutaneous circular open wound on female ratsfrom the macroscopic and histological point of view. 36 Wistar female rats scaling 250.0 grams on an average. On the 18 animais of the control group (Group 1) sodium chloride was injected intramuscularly into the left back member in an equivalent to the amount required for the group treated, immediately after making a skin open wound with a 6 mm diameter punch on the back of the animal at about 5 cm from the lower edges of the external ears. The same surgical procedure of the control group was carried out in the experimental group (Group 2), and methotrexate (1 mg/kg ofbody weight) was applied intramuscularly into the same anatomic location. They were killed on the 4th and 7th days after operation. The histologic alterations ofthe ejfect of methotrexate evidenced through optical microscopy, such as reduction of tissue area of granulation and necrosis (p < 0.001), the number of round nuclei (p < 0.001), in the number of fibroblasts and histiocytes in transformation (fusiform nuclei field) (p < 0.001), in the distance getween the edges of the wound (p < 0.001) and in the number of vessels/field (p < 0.05), were predominant on the 4th and 7th days. It is concluded that, the methotrexate attenuates the inflammatory answer actuating in the initial and fibroplasia phases, however, this has accelerated the reepitelization process. |
id |
INCA-1_e3337ea147864c9f0782a2c2264bbdf8 |
---|---|
oai_identifier_str |
oai:rbc.inca.gov.br:article/2885 |
network_acronym_str |
INCA-1 |
network_name_str |
Revista Brasileira de Cancerologia (Online) |
repository_id_str |
|
spelling |
Tissue reparation histology of open cutaneous wonds by metotrexate: experimental studies of female ratsAspectos histológicos da reparação tecidual por efeito do metotrexato em feridas cutâneas abertas: estudo experimental em ratasPeleCicatrização das FeridasHistologiaMetotrexatoEfeitos AdversosSkinWound HealingHistologyMethotrexateDrug EffectsThe authors have studied the action of methotrexate applied intramusculary on healing of cutaneous circular open wound on female ratsfrom the macroscopic and histological point of view. 36 Wistar female rats scaling 250.0 grams on an average. On the 18 animais of the control group (Group 1) sodium chloride was injected intramuscularly into the left back member in an equivalent to the amount required for the group treated, immediately after making a skin open wound with a 6 mm diameter punch on the back of the animal at about 5 cm from the lower edges of the external ears. The same surgical procedure of the control group was carried out in the experimental group (Group 2), and methotrexate (1 mg/kg ofbody weight) was applied intramuscularly into the same anatomic location. They were killed on the 4th and 7th days after operation. The histologic alterations ofthe ejfect of methotrexate evidenced through optical microscopy, such as reduction of tissue area of granulation and necrosis (p < 0.001), the number of round nuclei (p < 0.001), in the number of fibroblasts and histiocytes in transformation (fusiform nuclei field) (p < 0.001), in the distance getween the edges of the wound (p < 0.001) and in the number of vessels/field (p < 0.05), were predominant on the 4th and 7th days. It is concluded that, the methotrexate attenuates the inflammatory answer actuating in the initial and fibroplasia phases, however, this has accelerated the reepitelization process.Foi estudada a ação do metotrexato administrado por via intramuscular sobre a cicatrização de feridas cutâneas circulares abertas em ratas, do ponto de vista histológico. Foram operadas 36 ratas Wistar, com peso médio de 250,0 gramas. Nos 18 animais do grupo-controle (Grupo 1) foi injetado soro fisiológico 0,9% por via intramuscular, em volume equivalente ao necessário ao grupo tratado, no membro traseiro esquerdo, imediatamente após a confecção de uma ferida aberta de pele, provocada com vazador de 6 mm de diâmetro no dorso do animal, a cerca de 5 cm das bordas inferiores dos pavilhões auriculares. No grupo-experimento (Grupo 2) realizou-se o mesmo procedimento cirúrgico do grupo-controle e administrou-se metotrexato (1 mg/kg de peso corpóreo) por via intramuscular na mesma localização anatômica. Foram sacrificadas no 4“ e T dias de pós-operatório. As alterações histológicas do efeito do metotrexato, evidenciado através da microscopia óptica, se fizeram predominantemente no 7° dia de pós-operatório, tais como: a redução da área de tecido de granulação e necrose (p < 0,001), do número de núcleos redondos (p < 0,001), do número de polimorfonucleares (p < 0,001), do número de fibroblastos e histiócitos em transformação (núcleos fusiformes/campo) (p < 0,001), da distância entre as bordas da ferida (p < 0,001) e do número de vasos/campo (p < 0,005). Concluem que o metotrexato atenua a resposta inflamatória, atuando nas fases iniciais e de fibroplasia, entretanto, acelerou o processo de reepitelização.INCA2022-10-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtigos, Avaliado pelos paresapplication/pdfhttps://rbc.inca.gov.br/index.php/revista/article/view/288510.32635/2176-9745.RBC.1996v42n1.2885Revista Brasileira de Cancerologia; Vol. 42 No. 1 (1996): Jan./Feb./Mar.; 49-59Revista Brasileira de Cancerologia; Vol. 42 Núm. 1 (1996): ene./feb./mar.; 49-59Revista Brasileira de Cancerologia; v. 42 n. 1 (1996): jan./fev./mar.; 49-592176-9745reponame:Revista Brasileira de Cancerologia (Online)instname:Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA)instacron:INCAporhttps://rbc.inca.gov.br/index.php/revista/article/view/2885/1762https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCarneiro, Paulo Cesar AlvesGutman, Teresa Cristina FerreiraCastro, Onofre Ferreira deRodrigues, Pedro CarvalhoPinheiro, Juliano MoraesOliveira, Dilon Pinheiro de2023-06-19T13:18:58Zoai:rbc.inca.gov.br:article/2885Revistahttps://rbc.inca.gov.br/index.php/revistaPUBhttps://rbc.inca.gov.br/index.php/revista/oairbc@inca.gov.br0034-71162176-9745opendoar:2023-06-19T13:18:58Revista Brasileira de Cancerologia (Online) - Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA)false |
dc.title.none.fl_str_mv |
Tissue reparation histology of open cutaneous wonds by metotrexate: experimental studies of female rats Aspectos histológicos da reparação tecidual por efeito do metotrexato em feridas cutâneas abertas: estudo experimental em ratas |
title |
Tissue reparation histology of open cutaneous wonds by metotrexate: experimental studies of female rats |
spellingShingle |
Tissue reparation histology of open cutaneous wonds by metotrexate: experimental studies of female rats Carneiro, Paulo Cesar Alves Pele Cicatrização das Feridas Histologia Metotrexato Efeitos Adversos Skin Wound Healing Histology Methotrexate Drug Effects |
title_short |
Tissue reparation histology of open cutaneous wonds by metotrexate: experimental studies of female rats |
title_full |
Tissue reparation histology of open cutaneous wonds by metotrexate: experimental studies of female rats |
title_fullStr |
Tissue reparation histology of open cutaneous wonds by metotrexate: experimental studies of female rats |
title_full_unstemmed |
Tissue reparation histology of open cutaneous wonds by metotrexate: experimental studies of female rats |
title_sort |
Tissue reparation histology of open cutaneous wonds by metotrexate: experimental studies of female rats |
author |
Carneiro, Paulo Cesar Alves |
author_facet |
Carneiro, Paulo Cesar Alves Gutman, Teresa Cristina Ferreira Castro, Onofre Ferreira de Rodrigues, Pedro Carvalho Pinheiro, Juliano Moraes Oliveira, Dilon Pinheiro de |
author_role |
author |
author2 |
Gutman, Teresa Cristina Ferreira Castro, Onofre Ferreira de Rodrigues, Pedro Carvalho Pinheiro, Juliano Moraes Oliveira, Dilon Pinheiro de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Carneiro, Paulo Cesar Alves Gutman, Teresa Cristina Ferreira Castro, Onofre Ferreira de Rodrigues, Pedro Carvalho Pinheiro, Juliano Moraes Oliveira, Dilon Pinheiro de |
dc.subject.por.fl_str_mv |
Pele Cicatrização das Feridas Histologia Metotrexato Efeitos Adversos Skin Wound Healing Histology Methotrexate Drug Effects |
topic |
Pele Cicatrização das Feridas Histologia Metotrexato Efeitos Adversos Skin Wound Healing Histology Methotrexate Drug Effects |
description |
The authors have studied the action of methotrexate applied intramusculary on healing of cutaneous circular open wound on female ratsfrom the macroscopic and histological point of view. 36 Wistar female rats scaling 250.0 grams on an average. On the 18 animais of the control group (Group 1) sodium chloride was injected intramuscularly into the left back member in an equivalent to the amount required for the group treated, immediately after making a skin open wound with a 6 mm diameter punch on the back of the animal at about 5 cm from the lower edges of the external ears. The same surgical procedure of the control group was carried out in the experimental group (Group 2), and methotrexate (1 mg/kg ofbody weight) was applied intramuscularly into the same anatomic location. They were killed on the 4th and 7th days after operation. The histologic alterations ofthe ejfect of methotrexate evidenced through optical microscopy, such as reduction of tissue area of granulation and necrosis (p < 0.001), the number of round nuclei (p < 0.001), in the number of fibroblasts and histiocytes in transformation (fusiform nuclei field) (p < 0.001), in the distance getween the edges of the wound (p < 0.001) and in the number of vessels/field (p < 0.05), were predominant on the 4th and 7th days. It is concluded that, the methotrexate attenuates the inflammatory answer actuating in the initial and fibroplasia phases, however, this has accelerated the reepitelization process. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-10-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artigos, Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rbc.inca.gov.br/index.php/revista/article/view/2885 10.32635/2176-9745.RBC.1996v42n1.2885 |
url |
https://rbc.inca.gov.br/index.php/revista/article/view/2885 |
identifier_str_mv |
10.32635/2176-9745.RBC.1996v42n1.2885 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rbc.inca.gov.br/index.php/revista/article/view/2885/1762 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
INCA |
publisher.none.fl_str_mv |
INCA |
dc.source.none.fl_str_mv |
Revista Brasileira de Cancerologia; Vol. 42 No. 1 (1996): Jan./Feb./Mar.; 49-59 Revista Brasileira de Cancerologia; Vol. 42 Núm. 1 (1996): ene./feb./mar.; 49-59 Revista Brasileira de Cancerologia; v. 42 n. 1 (1996): jan./fev./mar.; 49-59 2176-9745 reponame:Revista Brasileira de Cancerologia (Online) instname:Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA) instacron:INCA |
instname_str |
Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA) |
instacron_str |
INCA |
institution |
INCA |
reponame_str |
Revista Brasileira de Cancerologia (Online) |
collection |
Revista Brasileira de Cancerologia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Cancerologia (Online) - Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA) |
repository.mail.fl_str_mv |
rbc@inca.gov.br |
_version_ |
1797042233331941376 |