Coexistence of cytotypes and chromosomal mosaicism in Hoplias malabaricus (Characiformes, Erythrinidae)
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INMETRO |
Texto Completo: | http://hdl.handle.net/10926/1764 |
Resumo: | Karyotypes of seventeen Hoplias malabaricus specimens, collected in the fish culture station of UNOPAR (University of Northern Paraná), were analyzed. The station is in the Claro River system in the Tibagi River basin. Two distinct and coexistent karyotype forms (cytotypes) were identified, comprising either 42 chromosomes (cytotype A) or 40 chromosomes (cytotype C), both presenting metacentric and submetacentric chromosomes. In two specimens, one male and one female, it was not possible to characterize a modal diploid number because different cell lines were observed, with a predominance of 2n=41 and 2n=42 chromosomes at a frequency of 38.24% and 41.12%, respectively. The karyotype with 2n=41 showed some putative monosomic and trisomic chromosomes, while the karyotype with 2n=42 showed 21 chromosomal pairs, similar to cytotype A. RAPD analysis showed that these two specimens have the same band profile of cytotype A (Nei’s genetic identity=92%), discarding a possible hybridization between both cytotypes and supporting the mosaicism hypothesis. These findings corroborate the isolation between cytotypes A and C. |
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info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCoexistence of cytotypes and chromosomal mosaicism in Hoplias malabaricus (Characiformes, Erythrinidae)20092012-06-27T19:00:25Z2012-06-27T19:00:25ZKaryotypes of seventeen Hoplias malabaricus specimens, collected in the fish culture station of UNOPAR (University of Northern Paraná), were analyzed. The station is in the Claro River system in the Tibagi River basin. Two distinct and coexistent karyotype forms (cytotypes) were identified, comprising either 42 chromosomes (cytotype A) or 40 chromosomes (cytotype C), both presenting metacentric and submetacentric chromosomes. In two specimens, one male and one female, it was not possible to characterize a modal diploid number because different cell lines were observed, with a predominance of 2n=41 and 2n=42 chromosomes at a frequency of 38.24% and 41.12%, respectively. The karyotype with 2n=41 showed some putative monosomic and trisomic chromosomes, while the karyotype with 2n=42 showed 21 chromosomal pairs, similar to cytotype A. RAPD analysis showed that these two specimens have the same band profile of cytotype A (Nei’s genetic identity=92%), discarding a possible hybridization between both cytotypes and supporting the mosaicism hypothesis. These findings corroborate the isolation between cytotypes A and C.8 p. : il.Submitted by Jonathan Barbosa (jon_crf@hotmail.com) on 2012-02-16T11:14:33Z No. of bitstreams: 1 Santos_2009.pdf: 1802408 bytes, checksum: 576eadc61c5395767c68683d9d31420b (MD5)Approved for entry into archive by Regina Mello(mrmello@inmetro.gov.br) on 2012-06-26T16:02:57Z (GMT) No. of bitstreams: 1 Santos_2009.pdf: 1802408 bytes, checksum: 576eadc61c5395767c68683d9d31420b (MD5)Made available in DSpace on 2012-06-27T19:00:25Z (GMT). No. of bitstreams: 1 Santos_2009.pdf: 1802408 bytes, checksum: 576eadc61c5395767c68683d9d31420b (MD5) Previous issue date: 2009enghttp://hdl.handle.net/10926/1764DMD_hdl_10926/1764ROSA, Renata da et al. Coexistence of cytotypes and chromosomal mosaicism in Hoplias malabaricus (Characiformes, Erythrinidae). Biological Research, v. 42, p. 289-296, 2009.Rosa, Renata daRubert, MarceléiaBertollo, Luiz Antonio CarlosSantos, Isabel Cristina Martins dosCaetano, Lucia Giulianoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional do INMETROinstname:Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)instacron:INMETRORosa_2009.pdfhttp://xrepo01s.inmetro.gov.br/bitstream/10926/1764/1/Rosa_2009.pdfapplication/pdf1802408http://xrepo01s.inmetro.gov.br/bitstream/10926/1764/1/Rosa_2009.pdf576eadc61c5395767c68683d9d31420bMD510926_1764_1Rosa_2009.pdf.txthttp://xrepo01s.inmetro.gov.br/bitstream/10926/1764/7/Rosa_2009.pdf.txttext/plain19636http://xrepo01s.inmetro.gov.br/bitstream/10926/1764/7/Rosa_2009.pdf.txt11a00561b3db08d7f122a08fd397f10dMD510926_1764_72024-06-10T15:24:45Zoai:xrepo01s.inmetro.gov.br:10926/1764Repositório de Publicaçõeshttp://repositorios.inmetro.gov.br/oai/requestopendoar:2013-04-26T18:29:24Repositório Institucional do INMETRO - Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)false |
dc.title.none.fl_str_mv |
Coexistence of cytotypes and chromosomal mosaicism in Hoplias malabaricus (Characiformes, Erythrinidae) |
title |
Coexistence of cytotypes and chromosomal mosaicism in Hoplias malabaricus (Characiformes, Erythrinidae) |
spellingShingle |
Coexistence of cytotypes and chromosomal mosaicism in Hoplias malabaricus (Characiformes, Erythrinidae) Rosa, Renata da |
title_short |
Coexistence of cytotypes and chromosomal mosaicism in Hoplias malabaricus (Characiformes, Erythrinidae) |
title_full |
Coexistence of cytotypes and chromosomal mosaicism in Hoplias malabaricus (Characiformes, Erythrinidae) |
title_fullStr |
Coexistence of cytotypes and chromosomal mosaicism in Hoplias malabaricus (Characiformes, Erythrinidae) |
title_full_unstemmed |
Coexistence of cytotypes and chromosomal mosaicism in Hoplias malabaricus (Characiformes, Erythrinidae) |
title_sort |
Coexistence of cytotypes and chromosomal mosaicism in Hoplias malabaricus (Characiformes, Erythrinidae) |
author |
Rosa, Renata da |
author_facet |
Rosa, Renata da Rubert, Marceléia Bertollo, Luiz Antonio Carlos Santos, Isabel Cristina Martins dos Caetano, Lucia Giuliano |
author_role |
author |
author2 |
Rubert, Marceléia Bertollo, Luiz Antonio Carlos Santos, Isabel Cristina Martins dos Caetano, Lucia Giuliano |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Rosa, Renata da Rubert, Marceléia Bertollo, Luiz Antonio Carlos Santos, Isabel Cristina Martins dos Caetano, Lucia Giuliano |
description |
Karyotypes of seventeen Hoplias malabaricus specimens, collected in the fish culture station of UNOPAR (University of Northern Paraná), were analyzed. The station is in the Claro River system in the Tibagi River basin. Two distinct and coexistent karyotype forms (cytotypes) were identified, comprising either 42 chromosomes (cytotype A) or 40 chromosomes (cytotype C), both presenting metacentric and submetacentric chromosomes. In two specimens, one male and one female, it was not possible to characterize a modal diploid number because different cell lines were observed, with a predominance of 2n=41 and 2n=42 chromosomes at a frequency of 38.24% and 41.12%, respectively. The karyotype with 2n=41 showed some putative monosomic and trisomic chromosomes, while the karyotype with 2n=42 showed 21 chromosomal pairs, similar to cytotype A. RAPD analysis showed that these two specimens have the same band profile of cytotype A (Nei’s genetic identity=92%), discarding a possible hybridization between both cytotypes and supporting the mosaicism hypothesis. These findings corroborate the isolation between cytotypes A and C. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009 |
dc.date.available.fl_str_mv |
2012-06-27T19:00:25Z |
dc.date.accessioned.fl_str_mv |
2012-06-27T19:00:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10926/1764 DMD_hdl_10926/1764 |
dc.identifier.citation.fl_str_mv |
ROSA, Renata da et al. Coexistence of cytotypes and chromosomal mosaicism in Hoplias malabaricus (Characiformes, Erythrinidae). Biological Research, v. 42, p. 289-296, 2009. |
url |
http://hdl.handle.net/10926/1764 |
identifier_str_mv |
DMD_hdl_10926/1764 ROSA, Renata da et al. Coexistence of cytotypes and chromosomal mosaicism in Hoplias malabaricus (Characiformes, Erythrinidae). Biological Research, v. 42, p. 289-296, 2009. |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.bitstream.fl_str_mv |
application/pdf text/plain |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional do INMETRO instname:Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO) instacron:INMETRO |
instname_str |
Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO) |
instacron_str |
INMETRO |
institution |
INMETRO |
reponame_str |
Repositório Institucional do INMETRO |
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Repositório Institucional do INMETRO |
repository.name.fl_str_mv |
Repositório Institucional do INMETRO - Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO) |
repository.mail.fl_str_mv |
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