Osteoblasts and bone marrow mesenchymal stromal cells control hematopoietic stem cell migration and proliferation in 3D In vitro model
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INMETRO |
Texto Completo: | http://hdl.handle.net/10926/1760 |
Resumo: | Background: Migration, proliferation, and differentiation of hematopoietic stem cells (HSCs) are dependent upon a complex three-dimensional (3D) bone marrow microenvironment. Although osteoblasts control the HSC pool, the subendosteal niche is complex and its cellular composition and the role of each cell population in HSC fate have not been established. In vivo models are complex and involve subtle species-specific differences, while bidimensional cultures do not reflect the 3D tissue organization. The aim of this study was to investigate in vitro the role of human bone marrow–derived mesenchymal stromal cells (BMSC) and active osteoblasts in control of migration, lodgment, and proliferation of HSCs. Methodology/Principal Findings: A complex mixed multicellular spheroid in vitro model was developed with human BMSC, undifferentiated or induced for one week into osteoblasts. A clear limit between the two stromal cells was established, and deposition of extracellular matrix proteins fibronectin, collagens I and IV, laminin, and osteopontin was similar to the observed in vivo. Noninduced BMSC cultured as spheroid expressed higher levels of mRNA for the chemokine CXCL12, and the growth factors Wnt5a and Kit ligand. Cord blood and bone marrow CD34+ cells moved in and out the spheroids, and some lodged at the interface of the two stromal cells. Myeloid colony-forming cells were maintained after seven days of coculture with mixed spheroids, and the frequency of cycling CD34+ cells was decreased. Conclusions/Significance: Undifferentiated and one-week osteo-induced BMSC self-assembled in a 3D spheroid and formed a microenvironment that is informative for hematopoietic progenitor cells, allowing their lodgment and controlling their proliferation. |
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info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleOsteoblasts and bone marrow mesenchymal stromal cells control hematopoietic stem cell migration and proliferation in 3D In vitro model20102012-06-27T18:58:57Z2012-06-27T18:58:57ZBackground: Migration, proliferation, and differentiation of hematopoietic stem cells (HSCs) are dependent upon a complex three-dimensional (3D) bone marrow microenvironment. Although osteoblasts control the HSC pool, the subendosteal niche is complex and its cellular composition and the role of each cell population in HSC fate have not been established. In vivo models are complex and involve subtle species-specific differences, while bidimensional cultures do not reflect the 3D tissue organization. The aim of this study was to investigate in vitro the role of human bone marrow–derived mesenchymal stromal cells (BMSC) and active osteoblasts in control of migration, lodgment, and proliferation of HSCs. Methodology/Principal Findings: A complex mixed multicellular spheroid in vitro model was developed with human BMSC, undifferentiated or induced for one week into osteoblasts. A clear limit between the two stromal cells was established, and deposition of extracellular matrix proteins fibronectin, collagens I and IV, laminin, and osteopontin was similar to the observed in vivo. Noninduced BMSC cultured as spheroid expressed higher levels of mRNA for the chemokine CXCL12, and the growth factors Wnt5a and Kit ligand. Cord blood and bone marrow CD34+ cells moved in and out the spheroids, and some lodged at the interface of the two stromal cells. Myeloid colony-forming cells were maintained after seven days of coculture with mixed spheroids, and the frequency of cycling CD34+ cells was decreased. Conclusions/Significance: Undifferentiated and one-week osteo-induced BMSC self-assembled in a 3D spheroid and formed a microenvironment that is informative for hematopoietic progenitor cells, allowing their lodgment and controlling their proliferation.13 p. : il.Submitted by Jonathan Barbosa (jon_crf@hotmail.com) on 2012-02-14T15:28:12Z No. of bitstreams: 1 Borojevic_2010.pdf: 1365228 bytes, checksum: edb7d547b19bcbbe5bcfc0677bd7ab44 (MD5)Approved for entry into archive by Regina Mello(mrmello@inmetro.gov.br) on 2012-06-26T15:55:55Z (GMT) No. of bitstreams: 1 Borojevic_2010.pdf: 1365228 bytes, checksum: edb7d547b19bcbbe5bcfc0677bd7ab44 (MD5)Made available in DSpace on 2012-06-27T18:58:57Z (GMT). No. of bitstreams: 1 Borojevic_2010.pdf: 1365228 bytes, checksum: edb7d547b19bcbbe5bcfc0677bd7ab44 (MD5) Previous issue date: 2010enghttp://hdl.handle.net/10926/1760DMD_hdl_10926/1760BARROS, Ana Paula D. N. de et al. Osteoblasts and bone marrow mesenchymal stromal cells control hematopoietic stem cell migration and proliferation in 3D in vitro model. Plos One, v. 5, 2010.Barros, Ana Paula Dantas Nunes deTakiya, Christina MaedaGarzoni, Luciana Lopes de Almeida RibeiroLeal-Ferreira, Mona LisaDutra, Hélio dos SantosChiarini, Luciana BarretoMeirelles, Maria de Nazareth Silveira Leal deBorojevic, RadovanRossi, Maria Isabel Doriainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional do INMETROinstname:Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)instacron:INMETROBarros_2010.pdfhttp://xrepo01s.inmetro.gov.br/bitstream/10926/1760/1/Barros_2010.pdfapplication/pdf1365228http://xrepo01s.inmetro.gov.br/bitstream/10926/1760/1/Barros_2010.pdfedb7d547b19bcbbe5bcfc0677bd7ab44MD510926_1760_12024-06-10T15:24:45Zoai:xrepo01s.inmetro.gov.br:10926/1760Repositório de Publicaçõeshttp://repositorios.inmetro.gov.br/oai/requestopendoar:2013-10-02T14:06:10Repositório Institucional do INMETRO - Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)false |
dc.title.none.fl_str_mv |
Osteoblasts and bone marrow mesenchymal stromal cells control hematopoietic stem cell migration and proliferation in 3D In vitro model |
title |
Osteoblasts and bone marrow mesenchymal stromal cells control hematopoietic stem cell migration and proliferation in 3D In vitro model |
spellingShingle |
Osteoblasts and bone marrow mesenchymal stromal cells control hematopoietic stem cell migration and proliferation in 3D In vitro model Barros, Ana Paula Dantas Nunes de |
title_short |
Osteoblasts and bone marrow mesenchymal stromal cells control hematopoietic stem cell migration and proliferation in 3D In vitro model |
title_full |
Osteoblasts and bone marrow mesenchymal stromal cells control hematopoietic stem cell migration and proliferation in 3D In vitro model |
title_fullStr |
Osteoblasts and bone marrow mesenchymal stromal cells control hematopoietic stem cell migration and proliferation in 3D In vitro model |
title_full_unstemmed |
Osteoblasts and bone marrow mesenchymal stromal cells control hematopoietic stem cell migration and proliferation in 3D In vitro model |
title_sort |
Osteoblasts and bone marrow mesenchymal stromal cells control hematopoietic stem cell migration and proliferation in 3D In vitro model |
author |
Barros, Ana Paula Dantas Nunes de |
author_facet |
Barros, Ana Paula Dantas Nunes de Takiya, Christina Maeda Garzoni, Luciana Lopes de Almeida Ribeiro Leal-Ferreira, Mona Lisa Dutra, Hélio dos Santos Chiarini, Luciana Barreto Meirelles, Maria de Nazareth Silveira Leal de Borojevic, Radovan Rossi, Maria Isabel Doria |
author_role |
author |
author2 |
Takiya, Christina Maeda Garzoni, Luciana Lopes de Almeida Ribeiro Leal-Ferreira, Mona Lisa Dutra, Hélio dos Santos Chiarini, Luciana Barreto Meirelles, Maria de Nazareth Silveira Leal de Borojevic, Radovan Rossi, Maria Isabel Doria |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Barros, Ana Paula Dantas Nunes de Takiya, Christina Maeda Garzoni, Luciana Lopes de Almeida Ribeiro Leal-Ferreira, Mona Lisa Dutra, Hélio dos Santos Chiarini, Luciana Barreto Meirelles, Maria de Nazareth Silveira Leal de Borojevic, Radovan Rossi, Maria Isabel Doria |
description |
Background: Migration, proliferation, and differentiation of hematopoietic stem cells (HSCs) are dependent upon a complex three-dimensional (3D) bone marrow microenvironment. Although osteoblasts control the HSC pool, the subendosteal niche is complex and its cellular composition and the role of each cell population in HSC fate have not been established. In vivo models are complex and involve subtle species-specific differences, while bidimensional cultures do not reflect the 3D tissue organization. The aim of this study was to investigate in vitro the role of human bone marrow–derived mesenchymal stromal cells (BMSC) and active osteoblasts in control of migration, lodgment, and proliferation of HSCs. Methodology/Principal Findings: A complex mixed multicellular spheroid in vitro model was developed with human BMSC, undifferentiated or induced for one week into osteoblasts. A clear limit between the two stromal cells was established, and deposition of extracellular matrix proteins fibronectin, collagens I and IV, laminin, and osteopontin was similar to the observed in vivo. Noninduced BMSC cultured as spheroid expressed higher levels of mRNA for the chemokine CXCL12, and the growth factors Wnt5a and Kit ligand. Cord blood and bone marrow CD34+ cells moved in and out the spheroids, and some lodged at the interface of the two stromal cells. Myeloid colony-forming cells were maintained after seven days of coculture with mixed spheroids, and the frequency of cycling CD34+ cells was decreased. Conclusions/Significance: Undifferentiated and one-week osteo-induced BMSC self-assembled in a 3D spheroid and formed a microenvironment that is informative for hematopoietic progenitor cells, allowing their lodgment and controlling their proliferation. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010 |
dc.date.available.fl_str_mv |
2012-06-27T18:58:57Z |
dc.date.accessioned.fl_str_mv |
2012-06-27T18:58:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10926/1760 DMD_hdl_10926/1760 |
dc.identifier.citation.fl_str_mv |
BARROS, Ana Paula D. N. de et al. Osteoblasts and bone marrow mesenchymal stromal cells control hematopoietic stem cell migration and proliferation in 3D in vitro model. Plos One, v. 5, 2010. |
url |
http://hdl.handle.net/10926/1760 |
identifier_str_mv |
DMD_hdl_10926/1760 BARROS, Ana Paula D. N. de et al. Osteoblasts and bone marrow mesenchymal stromal cells control hematopoietic stem cell migration and proliferation in 3D in vitro model. Plos One, v. 5, 2010. |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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openAccess |
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reponame:Repositório Institucional do INMETRO instname:Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO) instacron:INMETRO |
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Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO) |
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INMETRO |
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INMETRO |
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Repositório Institucional do INMETRO |
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Repositório Institucional do INMETRO - Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO) |
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