The Bunocephalus coracoideus species complex (Siluriformes, Aspredinidae). Signs of a speciation process through chromosomal, genetic and ecological diversity
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/15711 |
Resumo: | Bunocephalus is the most species-rich Aspredinidae genus, corresponding to a monophyletic clade with 13 valid species. However, many species have their classification put in question. Here, we analyzed individuals from four Amazonian populations of Bunocephalus coracoideus by cytogenetic and molecular procedures. The geographic distribution, genetic distances and karyotype data indicate that each population represents an Evolutionary Significant Unit (ESU). Cytogenetic markers showed distinct 2n and karyotype formulas, as well as different numbers and locations of the rDNA sites among ESUs. One of such populations (ESU-D) highlighted an extensive polymorphic condition, with several cytotypes probably due to chromosomal rearrangements and meiotic non-disjunctions. This resulted in several aneuploid karyotypes, which was also supported by the mapping of telomeric sequences. Phylograms based on Maximum Likelihood (ML) and Neighbor Joining (NJ) analyses grouped each ESU on particular highly supported clades, with the estimation of evolutionary divergence indicating values being higher than 3.8-12.3% among them. Our study reveals a huge degree of chromosomal and genetic diversity in B. coracoideus and highly points to the existence of four ESUs in allopatric and sympatric speciation processes. In fact, the high divergences found among the ESUs allowed us to delimitate lineages with taxonomic uncertainties in this nominal species. © 2017 Ferreira, Garcia, Matoso, de Jesus, Cioffi, Bertollo, Zuanon and Feldberg. |
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Ferreira, MilenaGarcia, CarolineMatoso, D. A.Jesus, Isac Silva deCioffi, Marcelo de BelloBertollo, Luiz Antônio CarlosZuanon, JansenFeldberg, Eliana2020-05-18T15:08:10Z2020-05-18T15:08:10Z2017https://repositorio.inpa.gov.br/handle/1/1571110.3389/fgene.2017.00120Bunocephalus is the most species-rich Aspredinidae genus, corresponding to a monophyletic clade with 13 valid species. However, many species have their classification put in question. Here, we analyzed individuals from four Amazonian populations of Bunocephalus coracoideus by cytogenetic and molecular procedures. The geographic distribution, genetic distances and karyotype data indicate that each population represents an Evolutionary Significant Unit (ESU). Cytogenetic markers showed distinct 2n and karyotype formulas, as well as different numbers and locations of the rDNA sites among ESUs. One of such populations (ESU-D) highlighted an extensive polymorphic condition, with several cytotypes probably due to chromosomal rearrangements and meiotic non-disjunctions. This resulted in several aneuploid karyotypes, which was also supported by the mapping of telomeric sequences. Phylograms based on Maximum Likelihood (ML) and Neighbor Joining (NJ) analyses grouped each ESU on particular highly supported clades, with the estimation of evolutionary divergence indicating values being higher than 3.8-12.3% among them. Our study reveals a huge degree of chromosomal and genetic diversity in B. coracoideus and highly points to the existence of four ESUs in allopatric and sympatric speciation processes. In fact, the high divergences found among the ESUs allowed us to delimitate lineages with taxonomic uncertainties in this nominal species. © 2017 Ferreira, Garcia, Matoso, de Jesus, Cioffi, Bertollo, Zuanon and Feldberg.Volume 8, Número SEPAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessDna 18sRibosome DnaRna 5sBiodiversityBunocephalus CoracoideusCatfishChromosome AnalysisCytogeneticsDna BarcodingDna DenaturationDna SequenceEvolutionIn Situ Hybridization, FluorescenceGene AmplificationGenetic VariabilityImage ProcessingKidney CellNonhumanPhylogenyPolymerase Chain ReactionTestisThe Bunocephalus coracoideus species complex (Siluriformes, Aspredinidae). Signs of a speciation process through chromosomal, genetic and ecological diversityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFrontiers in Geneticsengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfartigo-inpa.pdfapplication/pdf2707273https://repositorio.inpa.gov.br/bitstream/1/15711/1/artigo-inpa.pdfc3ef8c79da15e7c8aca4656281bbca3eMD511/157112020-05-18 11:20:07.072oai:repositorio:1/15711Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-05-18T15:20:07Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
The Bunocephalus coracoideus species complex (Siluriformes, Aspredinidae). Signs of a speciation process through chromosomal, genetic and ecological diversity |
title |
The Bunocephalus coracoideus species complex (Siluriformes, Aspredinidae). Signs of a speciation process through chromosomal, genetic and ecological diversity |
spellingShingle |
The Bunocephalus coracoideus species complex (Siluriformes, Aspredinidae). Signs of a speciation process through chromosomal, genetic and ecological diversity Ferreira, Milena Dna 18s Ribosome Dna Rna 5s Biodiversity Bunocephalus Coracoideus Catfish Chromosome Analysis Cytogenetics Dna Barcoding Dna Denaturation Dna Sequence Evolution In Situ Hybridization, Fluorescence Gene Amplification Genetic Variability Image Processing Kidney Cell Nonhuman Phylogeny Polymerase Chain Reaction Testis |
title_short |
The Bunocephalus coracoideus species complex (Siluriformes, Aspredinidae). Signs of a speciation process through chromosomal, genetic and ecological diversity |
title_full |
The Bunocephalus coracoideus species complex (Siluriformes, Aspredinidae). Signs of a speciation process through chromosomal, genetic and ecological diversity |
title_fullStr |
The Bunocephalus coracoideus species complex (Siluriformes, Aspredinidae). Signs of a speciation process through chromosomal, genetic and ecological diversity |
title_full_unstemmed |
The Bunocephalus coracoideus species complex (Siluriformes, Aspredinidae). Signs of a speciation process through chromosomal, genetic and ecological diversity |
title_sort |
The Bunocephalus coracoideus species complex (Siluriformes, Aspredinidae). Signs of a speciation process through chromosomal, genetic and ecological diversity |
author |
Ferreira, Milena |
author_facet |
Ferreira, Milena Garcia, Caroline Matoso, D. A. Jesus, Isac Silva de Cioffi, Marcelo de Bello Bertollo, Luiz Antônio Carlos Zuanon, Jansen Feldberg, Eliana |
author_role |
author |
author2 |
Garcia, Caroline Matoso, D. A. Jesus, Isac Silva de Cioffi, Marcelo de Bello Bertollo, Luiz Antônio Carlos Zuanon, Jansen Feldberg, Eliana |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Ferreira, Milena Garcia, Caroline Matoso, D. A. Jesus, Isac Silva de Cioffi, Marcelo de Bello Bertollo, Luiz Antônio Carlos Zuanon, Jansen Feldberg, Eliana |
dc.subject.eng.fl_str_mv |
Dna 18s Ribosome Dna Rna 5s Biodiversity Bunocephalus Coracoideus Catfish Chromosome Analysis Cytogenetics Dna Barcoding Dna Denaturation Dna Sequence Evolution In Situ Hybridization, Fluorescence Gene Amplification Genetic Variability Image Processing Kidney Cell Nonhuman Phylogeny Polymerase Chain Reaction Testis |
topic |
Dna 18s Ribosome Dna Rna 5s Biodiversity Bunocephalus Coracoideus Catfish Chromosome Analysis Cytogenetics Dna Barcoding Dna Denaturation Dna Sequence Evolution In Situ Hybridization, Fluorescence Gene Amplification Genetic Variability Image Processing Kidney Cell Nonhuman Phylogeny Polymerase Chain Reaction Testis |
description |
Bunocephalus is the most species-rich Aspredinidae genus, corresponding to a monophyletic clade with 13 valid species. However, many species have their classification put in question. Here, we analyzed individuals from four Amazonian populations of Bunocephalus coracoideus by cytogenetic and molecular procedures. The geographic distribution, genetic distances and karyotype data indicate that each population represents an Evolutionary Significant Unit (ESU). Cytogenetic markers showed distinct 2n and karyotype formulas, as well as different numbers and locations of the rDNA sites among ESUs. One of such populations (ESU-D) highlighted an extensive polymorphic condition, with several cytotypes probably due to chromosomal rearrangements and meiotic non-disjunctions. This resulted in several aneuploid karyotypes, which was also supported by the mapping of telomeric sequences. Phylograms based on Maximum Likelihood (ML) and Neighbor Joining (NJ) analyses grouped each ESU on particular highly supported clades, with the estimation of evolutionary divergence indicating values being higher than 3.8-12.3% among them. Our study reveals a huge degree of chromosomal and genetic diversity in B. coracoideus and highly points to the existence of four ESUs in allopatric and sympatric speciation processes. In fact, the high divergences found among the ESUs allowed us to delimitate lineages with taxonomic uncertainties in this nominal species. © 2017 Ferreira, Garcia, Matoso, de Jesus, Cioffi, Bertollo, Zuanon and Feldberg. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2020-05-18T15:08:10Z |
dc.date.available.fl_str_mv |
2020-05-18T15:08:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/15711 |
dc.identifier.doi.none.fl_str_mv |
10.3389/fgene.2017.00120 |
url |
https://repositorio.inpa.gov.br/handle/1/15711 |
identifier_str_mv |
10.3389/fgene.2017.00120 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 8, Número SEP |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Frontiers in Genetics |
publisher.none.fl_str_mv |
Frontiers in Genetics |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional do INPA instname:Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
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INPA |
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INPA |
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Repositório Institucional do INPA |
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Repositório Institucional do INPA |
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