Analysis of the intercaste transcriptional profile of Melipona scutellaris latreille, 1811 (hymenoptera, apidae, meliponini) by mRNA differential display

Detalhes bibliográficos
Autor(a) principal: Siquieroli, Ana Carolina Silva
Data de Publicação: 2009
Outros Autores: Vieira, Carlos Ueira, Carvalho-Zelse, Gislene A., Goulart, L. R., Kerr, Warwick Estevam, Bonetti, Ana Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional do INPA
Texto Completo: https://repositorio.inpa.gov.br/handle/1/16314
Resumo: In colonies of Melipona scutellaris Latreille, 1811 workers can be found with four ganglion nerve cells, a morphological characteristic of the queen. It is hypothesized that these workers, called intercastes, or phenocopies, are phenotypically-like workers, but genotypically identical to queens due to this specific trait. Workers with the same number of ganglion as queens seem to be intercastes between queens and workers. Our objective was to analyze the mRNA profiles of workers, queens, and intercastes of M. scutellaris through DDRT-PCR. Three hundred (300) pupae with white eyes were collected and externally identified according to the number of abdominal nerve ganglions: workers (5 ganglions), queens (4 ganglions) and intercastes (4 ganglions). The analysis identified differentially expressed transcripts that were present only in workers, but absent in intercastes and queens, confirming the hypothesis, by demonstrating the environmental effect on the queen genotype that generated phenotype-like workers.
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spelling Siquieroli, Ana Carolina SilvaVieira, Carlos UeiraCarvalho-Zelse, Gislene A.Goulart, L. R.Kerr, Warwick EstevamBonetti, Ana Maria2020-06-03T19:27:21Z2020-06-03T19:27:21Z2009https://repositorio.inpa.gov.br/handle/1/1631410.4067/S0716-97602009000100011In colonies of Melipona scutellaris Latreille, 1811 workers can be found with four ganglion nerve cells, a morphological characteristic of the queen. It is hypothesized that these workers, called intercastes, or phenocopies, are phenotypically-like workers, but genotypically identical to queens due to this specific trait. Workers with the same number of ganglion as queens seem to be intercastes between queens and workers. Our objective was to analyze the mRNA profiles of workers, queens, and intercastes of M. scutellaris through DDRT-PCR. Three hundred (300) pupae with white eyes were collected and externally identified according to the number of abdominal nerve ganglions: workers (5 ganglions), queens (4 ganglions) and intercastes (4 ganglions). The analysis identified differentially expressed transcripts that were present only in workers, but absent in intercastes and queens, confirming the hypothesis, by demonstrating the environmental effect on the queen genotype that generated phenotype-like workers.Volume 42, Número 1, Pags. 107-110Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessRna, MessengerAbdominal GanglionCell CountControlled StudyGene Expression ProfilingGenetic TranscriptionGenetic VariabilityInsect CasteMelipona ScutellarisNonhumanPupa (life Cycle Stage)Queen (insect)Reverse Transcription Polymerase Chain ReactionRna AnalysisSpecies ComparisonStingless BeeWorker (insect)ApidaeHymenopteraMeliponaMelipona ScutellarisMeliponinaeZosteropidaeAnalysis of the intercaste transcriptional profile of Melipona scutellaris latreille, 1811 (hymenoptera, apidae, meliponini) by mRNA differential displayinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBiological Researchengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALAnalysis.pdfAnalysis.pdfapplication/pdf138796https://repositorio.inpa.gov.br/bitstream/1/16314/1/Analysis.pdf533c6ee265102b462948c69e8d88edcdMD511/163142020-06-03 15:56:40.579oai:repositorio:1/16314Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-06-03T19:56:40Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.en.fl_str_mv Analysis of the intercaste transcriptional profile of Melipona scutellaris latreille, 1811 (hymenoptera, apidae, meliponini) by mRNA differential display
title Analysis of the intercaste transcriptional profile of Melipona scutellaris latreille, 1811 (hymenoptera, apidae, meliponini) by mRNA differential display
spellingShingle Analysis of the intercaste transcriptional profile of Melipona scutellaris latreille, 1811 (hymenoptera, apidae, meliponini) by mRNA differential display
Siquieroli, Ana Carolina Silva
Rna, Messenger
Abdominal Ganglion
Cell Count
Controlled Study
Gene Expression Profiling
Genetic Transcription
Genetic Variability
Insect Caste
Melipona Scutellaris
Nonhuman
Pupa (life Cycle Stage)
Queen (insect)
Reverse Transcription Polymerase Chain Reaction
Rna Analysis
Species Comparison
Stingless Bee
Worker (insect)
Apidae
Hymenoptera
Melipona
Melipona Scutellaris
Meliponinae
Zosteropidae
title_short Analysis of the intercaste transcriptional profile of Melipona scutellaris latreille, 1811 (hymenoptera, apidae, meliponini) by mRNA differential display
title_full Analysis of the intercaste transcriptional profile of Melipona scutellaris latreille, 1811 (hymenoptera, apidae, meliponini) by mRNA differential display
title_fullStr Analysis of the intercaste transcriptional profile of Melipona scutellaris latreille, 1811 (hymenoptera, apidae, meliponini) by mRNA differential display
title_full_unstemmed Analysis of the intercaste transcriptional profile of Melipona scutellaris latreille, 1811 (hymenoptera, apidae, meliponini) by mRNA differential display
title_sort Analysis of the intercaste transcriptional profile of Melipona scutellaris latreille, 1811 (hymenoptera, apidae, meliponini) by mRNA differential display
author Siquieroli, Ana Carolina Silva
author_facet Siquieroli, Ana Carolina Silva
Vieira, Carlos Ueira
Carvalho-Zelse, Gislene A.
Goulart, L. R.
Kerr, Warwick Estevam
Bonetti, Ana Maria
author_role author
author2 Vieira, Carlos Ueira
Carvalho-Zelse, Gislene A.
Goulart, L. R.
Kerr, Warwick Estevam
Bonetti, Ana Maria
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Siquieroli, Ana Carolina Silva
Vieira, Carlos Ueira
Carvalho-Zelse, Gislene A.
Goulart, L. R.
Kerr, Warwick Estevam
Bonetti, Ana Maria
dc.subject.eng.fl_str_mv Rna, Messenger
Abdominal Ganglion
Cell Count
Controlled Study
Gene Expression Profiling
Genetic Transcription
Genetic Variability
Insect Caste
Melipona Scutellaris
Nonhuman
Pupa (life Cycle Stage)
Queen (insect)
Reverse Transcription Polymerase Chain Reaction
Rna Analysis
Species Comparison
Stingless Bee
Worker (insect)
Apidae
Hymenoptera
Melipona
Melipona Scutellaris
Meliponinae
Zosteropidae
topic Rna, Messenger
Abdominal Ganglion
Cell Count
Controlled Study
Gene Expression Profiling
Genetic Transcription
Genetic Variability
Insect Caste
Melipona Scutellaris
Nonhuman
Pupa (life Cycle Stage)
Queen (insect)
Reverse Transcription Polymerase Chain Reaction
Rna Analysis
Species Comparison
Stingless Bee
Worker (insect)
Apidae
Hymenoptera
Melipona
Melipona Scutellaris
Meliponinae
Zosteropidae
description In colonies of Melipona scutellaris Latreille, 1811 workers can be found with four ganglion nerve cells, a morphological characteristic of the queen. It is hypothesized that these workers, called intercastes, or phenocopies, are phenotypically-like workers, but genotypically identical to queens due to this specific trait. Workers with the same number of ganglion as queens seem to be intercastes between queens and workers. Our objective was to analyze the mRNA profiles of workers, queens, and intercastes of M. scutellaris through DDRT-PCR. Three hundred (300) pupae with white eyes were collected and externally identified according to the number of abdominal nerve ganglions: workers (5 ganglions), queens (4 ganglions) and intercastes (4 ganglions). The analysis identified differentially expressed transcripts that were present only in workers, but absent in intercastes and queens, confirming the hypothesis, by demonstrating the environmental effect on the queen genotype that generated phenotype-like workers.
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2020-06-03T19:27:21Z
dc.date.available.fl_str_mv 2020-06-03T19:27:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.inpa.gov.br/handle/1/16314
dc.identifier.doi.none.fl_str_mv 10.4067/S0716-97602009000100011
url https://repositorio.inpa.gov.br/handle/1/16314
identifier_str_mv 10.4067/S0716-97602009000100011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Volume 42, Número 1, Pags. 107-110
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Biological Research
publisher.none.fl_str_mv Biological Research
dc.source.none.fl_str_mv reponame:Repositório Institucional do INPA
instname:Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron:INPA
instname_str Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron_str INPA
institution INPA
reponame_str Repositório Institucional do INPA
collection Repositório Institucional do INPA
bitstream.url.fl_str_mv https://repositorio.inpa.gov.br/bitstream/1/16314/1/Analysis.pdf
bitstream.checksum.fl_str_mv 533c6ee265102b462948c69e8d88edcd
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repository.name.fl_str_mv Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)
repository.mail.fl_str_mv
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