Antioxidant activity and peroxidase inhibition of Amazonian plants extracts traditionally used as anti-inflammatory

• Autor(a) principal: Vargas, Fabiano S. de
• Data de Publicação: 2016
• Outros Autores: Almeida, Patrícia Danielle Oliveira de, Boleti, Ana Paula A. de, Pereira, Maria de Meneses, Souza, Tatiane Pereira de, Vasconcellos, Marne Carvalho de, Nunez, C. V., Pohlit, Adrian Martin, Lima, Emerson Silva
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional do INPA
• Texto Completo: https://repositorio.inpa.gov.br/handle/1/15834
• topic: Antioxidant; Byrsonima Japurensis Plant Extract; Calycophyllum Spruceanum Plant Extract; Enzyme Inhibitor; Horseradish Peroxidase; Maytenus Guyanensis Plant Extract; Myeloperoxidase; Passiflora Nitida Plant Extract; Ptychopetalum Olacoides Plant Extract; Unclassified Drug; Antiinflammatory Agent; Antioxidant; Peroxidase; Plant Extract; Polyphenol; Animals Cell; Antiinflammatory Activity; Antioxidant Activity; Bleaching; Byrsonima Japurensis; Calycophyllum Spruceanum; Concentration Response; Controlled Study; Drug Screening; Enzyme Inhibition; Fibroblast; Maytenus Guyanensis; Medicinal Plant; Mouse; Nonhuman; Passiflora Nitida; Plant Stem; Ptychopetalum Olacoides; Animals; Antagonists And Inhibitors; Brasil; Chemistry; Human; Malpighiaceae; Maytenus; Nih 3t3 Cell Line; Olacaceae; Passiflora; Phytotherapy; Medicine, Traditional; Animal; Anti-inflammatory Agents; Antioxidants; Brasil; Humans; Malpighiaceae; Maytenus; Medicine, Traditional; Mice; Nih 3t3 Cells; Olacaceae; Passiflora; Peroxidase; Peroxidases; Phytotherapy; Plant Extracts; Plants, Medicinal; Polyphenols

Background: The Amazon is the largest rainforest in the world and is home to a rich biodiversity of medicinal plants. Several of these plants are used by the local population for the treatment of diseases, many of those with probable anti-inflammatory effect. The aim of the present investigation was to evaluate the in vitro antioxidant and anti-peroxidases potential of the ethanol extracts of five plants from the Brazilian Amazon (Byrsonima japurensis, Calycophyllum spruceanum, Maytenus guyanensis, Passiflora nitida and Ptychopetalum olacoides). Methods: DPPH, ABTS, superoxide anion radical, singlet oxygen and the β-carotene bleaching methods were employed for characterization of free radical scavenging activity. Also, total polyphenols were determined. Antioxidant activities were evaluated using murine fibroblast NIH3T3 cell. Inhibition of HRP and MPO were evaluated using amplex red® as susbtract. Results: The stem bark extracts of C. spruceanum and M. guyanensis provided the highest free radical scavenging activities. C. spruceanum exhibited IC50 = 7.5 ± 0.9, 5.0 ± 0.1, 18.2 ± 3.0 and 92.4 ± 24.8 μg/mL for DPPH·, ABTS+·, O2 -· and 1O2 assays, respectively. P. olacoides and C. spruceanum extracts also inhibited free radicals formation in the cell-based assay. At a concentration of 100 μg/mL, the extracts of C. spruceanum, B. japurensis inhibited horseradish peroxidase by 62 and 50 %, respectively. C. spruceanum, M. guyanensis, B. japurensis also inhibited myeloperoxidase in 72, 67 and 56 %, respectively. Conclusions: This work supports the folk use these species that inhibited peroxidases and exhibited significant free radical scavenging and antioxidant activities what can be related to treatment of inflammation. © 2016 de Vargas et al.