Cytogenetic analysis on pterophyllum scalare (perciformes, cichlidae) from jari river, pará state
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/16374 |
Resumo: | Cytogenetic studies were carried out on eighteen wild specimens of Pterophyllum scalare from Jari River, in Pará state, and the results were compared to literature. Mitotic chromosomes were obtained from kidney cells and the analysis was done using: C-banding, Ag-NOR staining, Chromomycin A3/DAPI sequential staining and fluorochrome in situ hybridization with human telomere probes. All individuals showed a chromosome number of 2n = 48 (12 M/SM and 36 ST/A) and FN = 60. No differences were detected between male and female karyotypes, indicating the absence of morphologically differentiated mitotic sexual chromosomes. Constitutive heterochromatin blocks were located at the centromeric and pericentromeric regions of all chromosomes. The largest submetacentric pair showed a differential staining on their short arms. Only two NOR bearing chromosomes were detected, and the stainings were observed at the distal region of the short arm of the largest chromosome pair, matching the secondary constriction. Chromomycin A3, stained the NOR and the centromeres of some chromosomes. DAPI-bands were observed at the centromeric regions of all chromosomes. Telomere sequences hybridised only at the terminal regions. © 2006 Taylor and Francis Group, LLC. |
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Barros, Regina Maria de SouzaPieczarka, Júlio CésarNagamachi, Cleusa YoshikoFeldberg, ElianaNascimento, Aline LCesar, Augusto C.G.Carvalho, Jaime Ribeiro2020-06-04T13:50:32Z2020-06-04T13:50:32Z2006https://repositorio.inpa.gov.br/handle/1/1637410.1080/00087114.2006.10797909Cytogenetic studies were carried out on eighteen wild specimens of Pterophyllum scalare from Jari River, in Pará state, and the results were compared to literature. Mitotic chromosomes were obtained from kidney cells and the analysis was done using: C-banding, Ag-NOR staining, Chromomycin A3/DAPI sequential staining and fluorochrome in situ hybridization with human telomere probes. All individuals showed a chromosome number of 2n = 48 (12 M/SM and 36 ST/A) and FN = 60. No differences were detected between male and female karyotypes, indicating the absence of morphologically differentiated mitotic sexual chromosomes. Constitutive heterochromatin blocks were located at the centromeric and pericentromeric regions of all chromosomes. The largest submetacentric pair showed a differential staining on their short arms. Only two NOR bearing chromosomes were detected, and the stainings were observed at the distal region of the short arm of the largest chromosome pair, matching the secondary constriction. Chromomycin A3, stained the NOR and the centromeres of some chromosomes. DAPI-bands were observed at the centromeric regions of all chromosomes. Telomere sequences hybridised only at the terminal regions. © 2006 Taylor and Francis Group, LLC.Volume 59, Número 2, Pags. 138-143Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessCichlidaePerciformesPterophyllum ScalareCytogenetic analysis on pterophyllum scalare (perciformes, cichlidae) from jari river, pará stateinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCaryologiaengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfartigo-inpa.pdfapplication/pdf1494684https://repositorio.inpa.gov.br/bitstream/1/16374/1/artigo-inpa.pdf93c8bb36f6ced4d513b750e4ecd97fb9MD511/163742020-07-14 11:36:30.872oai:repositorio:1/16374Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T15:36:30Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Cytogenetic analysis on pterophyllum scalare (perciformes, cichlidae) from jari river, pará state |
title |
Cytogenetic analysis on pterophyllum scalare (perciformes, cichlidae) from jari river, pará state |
spellingShingle |
Cytogenetic analysis on pterophyllum scalare (perciformes, cichlidae) from jari river, pará state Barros, Regina Maria de Souza Cichlidae Perciformes Pterophyllum Scalare |
title_short |
Cytogenetic analysis on pterophyllum scalare (perciformes, cichlidae) from jari river, pará state |
title_full |
Cytogenetic analysis on pterophyllum scalare (perciformes, cichlidae) from jari river, pará state |
title_fullStr |
Cytogenetic analysis on pterophyllum scalare (perciformes, cichlidae) from jari river, pará state |
title_full_unstemmed |
Cytogenetic analysis on pterophyllum scalare (perciformes, cichlidae) from jari river, pará state |
title_sort |
Cytogenetic analysis on pterophyllum scalare (perciformes, cichlidae) from jari river, pará state |
author |
Barros, Regina Maria de Souza |
author_facet |
Barros, Regina Maria de Souza Pieczarka, Júlio César Nagamachi, Cleusa Yoshiko Feldberg, Eliana Nascimento, Aline L Cesar, Augusto C.G. Carvalho, Jaime Ribeiro |
author_role |
author |
author2 |
Pieczarka, Júlio César Nagamachi, Cleusa Yoshiko Feldberg, Eliana Nascimento, Aline L Cesar, Augusto C.G. Carvalho, Jaime Ribeiro |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Barros, Regina Maria de Souza Pieczarka, Júlio César Nagamachi, Cleusa Yoshiko Feldberg, Eliana Nascimento, Aline L Cesar, Augusto C.G. Carvalho, Jaime Ribeiro |
dc.subject.eng.fl_str_mv |
Cichlidae Perciformes Pterophyllum Scalare |
topic |
Cichlidae Perciformes Pterophyllum Scalare |
description |
Cytogenetic studies were carried out on eighteen wild specimens of Pterophyllum scalare from Jari River, in Pará state, and the results were compared to literature. Mitotic chromosomes were obtained from kidney cells and the analysis was done using: C-banding, Ag-NOR staining, Chromomycin A3/DAPI sequential staining and fluorochrome in situ hybridization with human telomere probes. All individuals showed a chromosome number of 2n = 48 (12 M/SM and 36 ST/A) and FN = 60. No differences were detected between male and female karyotypes, indicating the absence of morphologically differentiated mitotic sexual chromosomes. Constitutive heterochromatin blocks were located at the centromeric and pericentromeric regions of all chromosomes. The largest submetacentric pair showed a differential staining on their short arms. Only two NOR bearing chromosomes were detected, and the stainings were observed at the distal region of the short arm of the largest chromosome pair, matching the secondary constriction. Chromomycin A3, stained the NOR and the centromeres of some chromosomes. DAPI-bands were observed at the centromeric regions of all chromosomes. Telomere sequences hybridised only at the terminal regions. © 2006 Taylor and Francis Group, LLC. |
publishDate |
2006 |
dc.date.issued.fl_str_mv |
2006 |
dc.date.accessioned.fl_str_mv |
2020-06-04T13:50:32Z |
dc.date.available.fl_str_mv |
2020-06-04T13:50:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/16374 |
dc.identifier.doi.none.fl_str_mv |
10.1080/00087114.2006.10797909 |
url |
https://repositorio.inpa.gov.br/handle/1/16374 |
identifier_str_mv |
10.1080/00087114.2006.10797909 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 59, Número 2, Pags. 138-143 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Caryologia |
publisher.none.fl_str_mv |
Caryologia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional do INPA instname:Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
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Instituto Nacional de Pesquisas da Amazônia (INPA) |
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INPA |
institution |
INPA |
reponame_str |
Repositório Institucional do INPA |
collection |
Repositório Institucional do INPA |
bitstream.url.fl_str_mv |
https://repositorio.inpa.gov.br/bitstream/1/16374/1/artigo-inpa.pdf |
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Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA) |
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