PCR in the diagnosis of cutaneous tuberculosis

Detalhes bibliográficos
Autor(a) principal: Ogusku, Maurício Morishi
Data de Publicação: 2003
Outros Autores: Sadahiro, Aya, Hirata, Mario Hiroyuki, Hirata, Rosario Domínguez Crespo, Zaitz, Clarisse, Salem, Júlia Ignez
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional do INPA
Texto Completo: https://repositorio.inpa.gov.br/handle/1/16407
Resumo: Seeking to improve the laboratory diagnosis of Cutaneous Tuberculosis, a study was carried out on the application of PCR technique in macerated, decontaminated (with 4% H2SO4 for elimination of normal microbiot), neutralized (with 4% NaOH) biopsies tissues samples stored at -20°C. Of the 37 samples submitted for study, 16.22% were positive by microscopy for acid-fast bacilli (concentrated method) and in 43.24% the Mycobacterium tuberculosis was isolated in Löwenstein-Jensen medium. Using a M. tuberculosis complex specific primer set (gene sequence for 16S rDNA), the mycobacterial DNA was detected in 24.32% of the biopsies. The sensitivity and specificity of PCR were 43.7% and 90.4%, respectively. Due to low sensitivity and discrepant results between bacteriological techniques and PCR methodology, the samples were repeated in a new PCR with primers for the IS6110 target. The sensitivity and specificity of PCR for the IS6110 target obtained 100% in comparison with the culture method. The results confirm the effectiveness of PCR methodology using primers for the IS6110 gene sequence and permit the PCR method to be applied to frozen cutaneous biopsies sent by services that do not identify the M. tuberculosis by the biology molecular method.
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spelling Ogusku, Maurício MorishiSadahiro, AyaHirata, Mario HiroyukiHirata, Rosario Domínguez CrespoZaitz, ClarisseSalem, Júlia Ignez2020-06-04T15:17:41Z2020-06-04T15:17:41Z2003https://repositorio.inpa.gov.br/handle/1/1640710.1590/S1517-83822003000200015Seeking to improve the laboratory diagnosis of Cutaneous Tuberculosis, a study was carried out on the application of PCR technique in macerated, decontaminated (with 4% H2SO4 for elimination of normal microbiot), neutralized (with 4% NaOH) biopsies tissues samples stored at -20°C. Of the 37 samples submitted for study, 16.22% were positive by microscopy for acid-fast bacilli (concentrated method) and in 43.24% the Mycobacterium tuberculosis was isolated in Löwenstein-Jensen medium. Using a M. tuberculosis complex specific primer set (gene sequence for 16S rDNA), the mycobacterial DNA was detected in 24.32% of the biopsies. The sensitivity and specificity of PCR were 43.7% and 90.4%, respectively. Due to low sensitivity and discrepant results between bacteriological techniques and PCR methodology, the samples were repeated in a new PCR with primers for the IS6110 target. The sensitivity and specificity of PCR for the IS6110 target obtained 100% in comparison with the culture method. The results confirm the effectiveness of PCR methodology using primers for the IS6110 gene sequence and permit the PCR method to be applied to frozen cutaneous biopsies sent by services that do not identify the M. tuberculosis by the biology molecular method.Volume 34, Número 2, Pags. 165-170Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessBacilliBacteria (microorganisms)MycobacteriumMycobacterium TuberculosisPCR in the diagnosis of cutaneous tuberculosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrazilian Journal of Microbiologyengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfartigo-inpa.pdfapplication/pdf708603https://repositorio.inpa.gov.br/bitstream/1/16407/1/artigo-inpa.pdf2d3dbcb09e246a1887d2a4727d8127e6MD511/164072020-06-04 11:35:09.091oai:repositorio:1/16407Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-06-04T15:35:09Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.en.fl_str_mv PCR in the diagnosis of cutaneous tuberculosis
title PCR in the diagnosis of cutaneous tuberculosis
spellingShingle PCR in the diagnosis of cutaneous tuberculosis
Ogusku, Maurício Morishi
Bacilli
Bacteria (microorganisms)
Mycobacterium
Mycobacterium Tuberculosis
title_short PCR in the diagnosis of cutaneous tuberculosis
title_full PCR in the diagnosis of cutaneous tuberculosis
title_fullStr PCR in the diagnosis of cutaneous tuberculosis
title_full_unstemmed PCR in the diagnosis of cutaneous tuberculosis
title_sort PCR in the diagnosis of cutaneous tuberculosis
author Ogusku, Maurício Morishi
author_facet Ogusku, Maurício Morishi
Sadahiro, Aya
Hirata, Mario Hiroyuki
Hirata, Rosario Domínguez Crespo
Zaitz, Clarisse
Salem, Júlia Ignez
author_role author
author2 Sadahiro, Aya
Hirata, Mario Hiroyuki
Hirata, Rosario Domínguez Crespo
Zaitz, Clarisse
Salem, Júlia Ignez
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Ogusku, Maurício Morishi
Sadahiro, Aya
Hirata, Mario Hiroyuki
Hirata, Rosario Domínguez Crespo
Zaitz, Clarisse
Salem, Júlia Ignez
dc.subject.eng.fl_str_mv Bacilli
Bacteria (microorganisms)
Mycobacterium
Mycobacterium Tuberculosis
topic Bacilli
Bacteria (microorganisms)
Mycobacterium
Mycobacterium Tuberculosis
description Seeking to improve the laboratory diagnosis of Cutaneous Tuberculosis, a study was carried out on the application of PCR technique in macerated, decontaminated (with 4% H2SO4 for elimination of normal microbiot), neutralized (with 4% NaOH) biopsies tissues samples stored at -20°C. Of the 37 samples submitted for study, 16.22% were positive by microscopy for acid-fast bacilli (concentrated method) and in 43.24% the Mycobacterium tuberculosis was isolated in Löwenstein-Jensen medium. Using a M. tuberculosis complex specific primer set (gene sequence for 16S rDNA), the mycobacterial DNA was detected in 24.32% of the biopsies. The sensitivity and specificity of PCR were 43.7% and 90.4%, respectively. Due to low sensitivity and discrepant results between bacteriological techniques and PCR methodology, the samples were repeated in a new PCR with primers for the IS6110 target. The sensitivity and specificity of PCR for the IS6110 target obtained 100% in comparison with the culture method. The results confirm the effectiveness of PCR methodology using primers for the IS6110 gene sequence and permit the PCR method to be applied to frozen cutaneous biopsies sent by services that do not identify the M. tuberculosis by the biology molecular method.
publishDate 2003
dc.date.issued.fl_str_mv 2003
dc.date.accessioned.fl_str_mv 2020-06-04T15:17:41Z
dc.date.available.fl_str_mv 2020-06-04T15:17:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv https://repositorio.inpa.gov.br/handle/1/16407
dc.identifier.doi.none.fl_str_mv 10.1590/S1517-83822003000200015
url https://repositorio.inpa.gov.br/handle/1/16407
identifier_str_mv 10.1590/S1517-83822003000200015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Volume 34, Número 2, Pags. 165-170
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Brazilian Journal of Microbiology
publisher.none.fl_str_mv Brazilian Journal of Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional do INPA
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institution INPA
reponame_str Repositório Institucional do INPA
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