PCR in the diagnosis of cutaneous tuberculosis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2003 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/16407 |
Resumo: | Seeking to improve the laboratory diagnosis of Cutaneous Tuberculosis, a study was carried out on the application of PCR technique in macerated, decontaminated (with 4% H2SO4 for elimination of normal microbiot), neutralized (with 4% NaOH) biopsies tissues samples stored at -20°C. Of the 37 samples submitted for study, 16.22% were positive by microscopy for acid-fast bacilli (concentrated method) and in 43.24% the Mycobacterium tuberculosis was isolated in Löwenstein-Jensen medium. Using a M. tuberculosis complex specific primer set (gene sequence for 16S rDNA), the mycobacterial DNA was detected in 24.32% of the biopsies. The sensitivity and specificity of PCR were 43.7% and 90.4%, respectively. Due to low sensitivity and discrepant results between bacteriological techniques and PCR methodology, the samples were repeated in a new PCR with primers for the IS6110 target. The sensitivity and specificity of PCR for the IS6110 target obtained 100% in comparison with the culture method. The results confirm the effectiveness of PCR methodology using primers for the IS6110 gene sequence and permit the PCR method to be applied to frozen cutaneous biopsies sent by services that do not identify the M. tuberculosis by the biology molecular method. |
id |
INPA-2_3c8db39710f324b997bcc86201e706ef |
---|---|
oai_identifier_str |
oai:repositorio:1/16407 |
network_acronym_str |
INPA-2 |
network_name_str |
Repositório Institucional do INPA |
repository_id_str |
|
spelling |
Ogusku, Maurício MorishiSadahiro, AyaHirata, Mario HiroyukiHirata, Rosario Domínguez CrespoZaitz, ClarisseSalem, Júlia Ignez2020-06-04T15:17:41Z2020-06-04T15:17:41Z2003https://repositorio.inpa.gov.br/handle/1/1640710.1590/S1517-83822003000200015Seeking to improve the laboratory diagnosis of Cutaneous Tuberculosis, a study was carried out on the application of PCR technique in macerated, decontaminated (with 4% H2SO4 for elimination of normal microbiot), neutralized (with 4% NaOH) biopsies tissues samples stored at -20°C. Of the 37 samples submitted for study, 16.22% were positive by microscopy for acid-fast bacilli (concentrated method) and in 43.24% the Mycobacterium tuberculosis was isolated in Löwenstein-Jensen medium. Using a M. tuberculosis complex specific primer set (gene sequence for 16S rDNA), the mycobacterial DNA was detected in 24.32% of the biopsies. The sensitivity and specificity of PCR were 43.7% and 90.4%, respectively. Due to low sensitivity and discrepant results between bacteriological techniques and PCR methodology, the samples were repeated in a new PCR with primers for the IS6110 target. The sensitivity and specificity of PCR for the IS6110 target obtained 100% in comparison with the culture method. The results confirm the effectiveness of PCR methodology using primers for the IS6110 gene sequence and permit the PCR method to be applied to frozen cutaneous biopsies sent by services that do not identify the M. tuberculosis by the biology molecular method.Volume 34, Número 2, Pags. 165-170Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessBacilliBacteria (microorganisms)MycobacteriumMycobacterium TuberculosisPCR in the diagnosis of cutaneous tuberculosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrazilian Journal of Microbiologyengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfartigo-inpa.pdfapplication/pdf708603https://repositorio.inpa.gov.br/bitstream/1/16407/1/artigo-inpa.pdf2d3dbcb09e246a1887d2a4727d8127e6MD511/164072020-06-04 11:35:09.091oai:repositorio:1/16407Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-06-04T15:35:09Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
PCR in the diagnosis of cutaneous tuberculosis |
title |
PCR in the diagnosis of cutaneous tuberculosis |
spellingShingle |
PCR in the diagnosis of cutaneous tuberculosis Ogusku, Maurício Morishi Bacilli Bacteria (microorganisms) Mycobacterium Mycobacterium Tuberculosis |
title_short |
PCR in the diagnosis of cutaneous tuberculosis |
title_full |
PCR in the diagnosis of cutaneous tuberculosis |
title_fullStr |
PCR in the diagnosis of cutaneous tuberculosis |
title_full_unstemmed |
PCR in the diagnosis of cutaneous tuberculosis |
title_sort |
PCR in the diagnosis of cutaneous tuberculosis |
author |
Ogusku, Maurício Morishi |
author_facet |
Ogusku, Maurício Morishi Sadahiro, Aya Hirata, Mario Hiroyuki Hirata, Rosario Domínguez Crespo Zaitz, Clarisse Salem, Júlia Ignez |
author_role |
author |
author2 |
Sadahiro, Aya Hirata, Mario Hiroyuki Hirata, Rosario Domínguez Crespo Zaitz, Clarisse Salem, Júlia Ignez |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Ogusku, Maurício Morishi Sadahiro, Aya Hirata, Mario Hiroyuki Hirata, Rosario Domínguez Crespo Zaitz, Clarisse Salem, Júlia Ignez |
dc.subject.eng.fl_str_mv |
Bacilli Bacteria (microorganisms) Mycobacterium Mycobacterium Tuberculosis |
topic |
Bacilli Bacteria (microorganisms) Mycobacterium Mycobacterium Tuberculosis |
description |
Seeking to improve the laboratory diagnosis of Cutaneous Tuberculosis, a study was carried out on the application of PCR technique in macerated, decontaminated (with 4% H2SO4 for elimination of normal microbiot), neutralized (with 4% NaOH) biopsies tissues samples stored at -20°C. Of the 37 samples submitted for study, 16.22% were positive by microscopy for acid-fast bacilli (concentrated method) and in 43.24% the Mycobacterium tuberculosis was isolated in Löwenstein-Jensen medium. Using a M. tuberculosis complex specific primer set (gene sequence for 16S rDNA), the mycobacterial DNA was detected in 24.32% of the biopsies. The sensitivity and specificity of PCR were 43.7% and 90.4%, respectively. Due to low sensitivity and discrepant results between bacteriological techniques and PCR methodology, the samples were repeated in a new PCR with primers for the IS6110 target. The sensitivity and specificity of PCR for the IS6110 target obtained 100% in comparison with the culture method. The results confirm the effectiveness of PCR methodology using primers for the IS6110 gene sequence and permit the PCR method to be applied to frozen cutaneous biopsies sent by services that do not identify the M. tuberculosis by the biology molecular method. |
publishDate |
2003 |
dc.date.issued.fl_str_mv |
2003 |
dc.date.accessioned.fl_str_mv |
2020-06-04T15:17:41Z |
dc.date.available.fl_str_mv |
2020-06-04T15:17:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/16407 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S1517-83822003000200015 |
url |
https://repositorio.inpa.gov.br/handle/1/16407 |
identifier_str_mv |
10.1590/S1517-83822003000200015 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 34, Número 2, Pags. 165-170 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Brazilian Journal of Microbiology |
publisher.none.fl_str_mv |
Brazilian Journal of Microbiology |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional do INPA instname:Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
instname_str |
Instituto Nacional de Pesquisas da Amazônia (INPA) |
instacron_str |
INPA |
institution |
INPA |
reponame_str |
Repositório Institucional do INPA |
collection |
Repositório Institucional do INPA |
bitstream.url.fl_str_mv |
https://repositorio.inpa.gov.br/bitstream/1/16407/1/artigo-inpa.pdf |
bitstream.checksum.fl_str_mv |
2d3dbcb09e246a1887d2a4727d8127e6 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 |
repository.name.fl_str_mv |
Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA) |
repository.mail.fl_str_mv |
|
_version_ |
1809928882271289344 |