Ecophysiology, genotoxicity, histopathology, and gene responses of naphthalene injected colossoma macropomum (Cuvier, 1818) exposed to hypoxia

Detalhes bibliográficos
Autor(a) principal: Souza, Samara Silva de
Data de Publicação: 2019
Outros Autores: Silva, Grazyelle Sebrenski da, Almeida-Val, Vera Maria Fonseca de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional do INPA
Texto Completo: https://repositorio.inpa.gov.br/handle/1/14963
Resumo: The present study aimed to evaluate the biological responses of Colossoma macropomum to naphthalene injection and subsequent hypoxia exposure, emphasizing the expression of the tumor suppressor gene tp53. Tambaquis were intraperitoneally injected with naphthalene (50 mg/kg) and, after 96 hours, the fish were transferred to respirometry chambers and, submitted to progressive hypoxia for the determination of critical PO2. In a subsequent experiment, the fish received an intraperitoneal injection of naphthalene and were kept for 96 hours under normoxia. Successively, fish were challenged with acute hypoxia (PO2 <PO2 crit) during 6 hours. We observed that the PO2 crit was not affected by naphthalene injection. Moreover, hematological parameters were modulated only in response to hypoxia. Fish with naphthalene injection plus hypoxia exposure presented altered activity of the GST and CAT enzymes. Exposure to naphthalene also resulted in DNA damages, which was not influenced by hypoxia. Hypoxia accentuated the hepatic lesions caused by naphthalene, as well as it also impaired the transcription of tp53 in naphtalene injected fish, demonstrating the risks of contaminating aquatic environments, especially environments where hypoxic conditions are common and occur on a daily or on seasonal basis, as in the Amazon basin. © 2019, Sociedade Brasileira de Genética.
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spelling Souza, Samara Silva deSilva, Grazyelle Sebrenski daAlmeida-Val, Vera Maria Fonseca de2020-05-07T13:59:10Z2020-05-07T13:59:10Z2019https://repositorio.inpa.gov.br/handle/1/1496310.1590/1678-4685-gmb-2018-0084The present study aimed to evaluate the biological responses of Colossoma macropomum to naphthalene injection and subsequent hypoxia exposure, emphasizing the expression of the tumor suppressor gene tp53. Tambaquis were intraperitoneally injected with naphthalene (50 mg/kg) and, after 96 hours, the fish were transferred to respirometry chambers and, submitted to progressive hypoxia for the determination of critical PO2. In a subsequent experiment, the fish received an intraperitoneal injection of naphthalene and were kept for 96 hours under normoxia. Successively, fish were challenged with acute hypoxia (PO2 <PO2 crit) during 6 hours. We observed that the PO2 crit was not affected by naphthalene injection. Moreover, hematological parameters were modulated only in response to hypoxia. Fish with naphthalene injection plus hypoxia exposure presented altered activity of the GST and CAT enzymes. Exposure to naphthalene also resulted in DNA damages, which was not influenced by hypoxia. Hypoxia accentuated the hepatic lesions caused by naphthalene, as well as it also impaired the transcription of tp53 in naphtalene injected fish, demonstrating the risks of contaminating aquatic environments, especially environments where hypoxic conditions are common and occur on a daily or on seasonal basis, as in the Amazon basin. © 2019, Sociedade Brasileira de Genética.Volume 42, Número 2, Pags. 411-424Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessCatalaseGlutathione TransferaseNaphthaleneAnimals ExperimentAnimals ModelAnimals TissueBiochemical AnalysisColossoma MacropomumControlled StudyDna DamageEcophysiologyGene ExpressionGenetic TranscriptionGenotoxicityHematological ParametersHistopathologyHypoxiaLipid PeroxidationLiver InjuryNonhumanOxygen TensionTumor Suppressor GeneEcophysiology, genotoxicity, histopathology, and gene responses of naphthalene injected colossoma macropomum (Cuvier, 1818) exposed to hypoxiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Biologyengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfapplication/pdf2287479https://repositorio.inpa.gov.br/bitstream/1/14963/1/artigo-inpa.pdf749334b64e8dc9ab820c4dac529a97e7MD51CC-LICENSElicense_rdfapplication/octet-stream914https://repositorio.inpa.gov.br/bitstream/1/14963/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD521/149632020-07-14 10:30:02.399oai:repositorio:1/14963Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T14:30:02Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.en.fl_str_mv Ecophysiology, genotoxicity, histopathology, and gene responses of naphthalene injected colossoma macropomum (Cuvier, 1818) exposed to hypoxia
title Ecophysiology, genotoxicity, histopathology, and gene responses of naphthalene injected colossoma macropomum (Cuvier, 1818) exposed to hypoxia
spellingShingle Ecophysiology, genotoxicity, histopathology, and gene responses of naphthalene injected colossoma macropomum (Cuvier, 1818) exposed to hypoxia
Souza, Samara Silva de
Catalase
Glutathione Transferase
Naphthalene
Animals Experiment
Animals Model
Animals Tissue
Biochemical Analysis
Colossoma Macropomum
Controlled Study
Dna Damage
Ecophysiology
Gene Expression
Genetic Transcription
Genotoxicity
Hematological Parameters
Histopathology
Hypoxia
Lipid Peroxidation
Liver Injury
Nonhuman
Oxygen Tension
Tumor Suppressor Gene
title_short Ecophysiology, genotoxicity, histopathology, and gene responses of naphthalene injected colossoma macropomum (Cuvier, 1818) exposed to hypoxia
title_full Ecophysiology, genotoxicity, histopathology, and gene responses of naphthalene injected colossoma macropomum (Cuvier, 1818) exposed to hypoxia
title_fullStr Ecophysiology, genotoxicity, histopathology, and gene responses of naphthalene injected colossoma macropomum (Cuvier, 1818) exposed to hypoxia
title_full_unstemmed Ecophysiology, genotoxicity, histopathology, and gene responses of naphthalene injected colossoma macropomum (Cuvier, 1818) exposed to hypoxia
title_sort Ecophysiology, genotoxicity, histopathology, and gene responses of naphthalene injected colossoma macropomum (Cuvier, 1818) exposed to hypoxia
author Souza, Samara Silva de
author_facet Souza, Samara Silva de
Silva, Grazyelle Sebrenski da
Almeida-Val, Vera Maria Fonseca de
author_role author
author2 Silva, Grazyelle Sebrenski da
Almeida-Val, Vera Maria Fonseca de
author2_role author
author
dc.contributor.author.fl_str_mv Souza, Samara Silva de
Silva, Grazyelle Sebrenski da
Almeida-Val, Vera Maria Fonseca de
dc.subject.eng.fl_str_mv Catalase
Glutathione Transferase
Naphthalene
Animals Experiment
Animals Model
Animals Tissue
Biochemical Analysis
Colossoma Macropomum
Controlled Study
Dna Damage
Ecophysiology
Gene Expression
Genetic Transcription
Genotoxicity
Hematological Parameters
Histopathology
Hypoxia
Lipid Peroxidation
Liver Injury
Nonhuman
Oxygen Tension
Tumor Suppressor Gene
topic Catalase
Glutathione Transferase
Naphthalene
Animals Experiment
Animals Model
Animals Tissue
Biochemical Analysis
Colossoma Macropomum
Controlled Study
Dna Damage
Ecophysiology
Gene Expression
Genetic Transcription
Genotoxicity
Hematological Parameters
Histopathology
Hypoxia
Lipid Peroxidation
Liver Injury
Nonhuman
Oxygen Tension
Tumor Suppressor Gene
description The present study aimed to evaluate the biological responses of Colossoma macropomum to naphthalene injection and subsequent hypoxia exposure, emphasizing the expression of the tumor suppressor gene tp53. Tambaquis were intraperitoneally injected with naphthalene (50 mg/kg) and, after 96 hours, the fish were transferred to respirometry chambers and, submitted to progressive hypoxia for the determination of critical PO2. In a subsequent experiment, the fish received an intraperitoneal injection of naphthalene and were kept for 96 hours under normoxia. Successively, fish were challenged with acute hypoxia (PO2 <PO2 crit) during 6 hours. We observed that the PO2 crit was not affected by naphthalene injection. Moreover, hematological parameters were modulated only in response to hypoxia. Fish with naphthalene injection plus hypoxia exposure presented altered activity of the GST and CAT enzymes. Exposure to naphthalene also resulted in DNA damages, which was not influenced by hypoxia. Hypoxia accentuated the hepatic lesions caused by naphthalene, as well as it also impaired the transcription of tp53 in naphtalene injected fish, demonstrating the risks of contaminating aquatic environments, especially environments where hypoxic conditions are common and occur on a daily or on seasonal basis, as in the Amazon basin. © 2019, Sociedade Brasileira de Genética.
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2020-05-07T13:59:10Z
dc.date.available.fl_str_mv 2020-05-07T13:59:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.inpa.gov.br/handle/1/14963
dc.identifier.doi.none.fl_str_mv 10.1590/1678-4685-gmb-2018-0084
url https://repositorio.inpa.gov.br/handle/1/14963
identifier_str_mv 10.1590/1678-4685-gmb-2018-0084
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Volume 42, Número 2, Pags. 411-424
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Genetics and Molecular Biology
publisher.none.fl_str_mv Genetics and Molecular Biology
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