Ecophysiology, genotoxicity, histopathology, and gene responses of naphthalene injected colossoma macropomum (Cuvier, 1818) exposed to hypoxia
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/14963 |
Resumo: | The present study aimed to evaluate the biological responses of Colossoma macropomum to naphthalene injection and subsequent hypoxia exposure, emphasizing the expression of the tumor suppressor gene tp53. Tambaquis were intraperitoneally injected with naphthalene (50 mg/kg) and, after 96 hours, the fish were transferred to respirometry chambers and, submitted to progressive hypoxia for the determination of critical PO2. In a subsequent experiment, the fish received an intraperitoneal injection of naphthalene and were kept for 96 hours under normoxia. Successively, fish were challenged with acute hypoxia (PO2 <PO2 crit) during 6 hours. We observed that the PO2 crit was not affected by naphthalene injection. Moreover, hematological parameters were modulated only in response to hypoxia. Fish with naphthalene injection plus hypoxia exposure presented altered activity of the GST and CAT enzymes. Exposure to naphthalene also resulted in DNA damages, which was not influenced by hypoxia. Hypoxia accentuated the hepatic lesions caused by naphthalene, as well as it also impaired the transcription of tp53 in naphtalene injected fish, demonstrating the risks of contaminating aquatic environments, especially environments where hypoxic conditions are common and occur on a daily or on seasonal basis, as in the Amazon basin. © 2019, Sociedade Brasileira de Genética. |
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Souza, Samara Silva deSilva, Grazyelle Sebrenski daAlmeida-Val, Vera Maria Fonseca de2020-05-07T13:59:10Z2020-05-07T13:59:10Z2019https://repositorio.inpa.gov.br/handle/1/1496310.1590/1678-4685-gmb-2018-0084The present study aimed to evaluate the biological responses of Colossoma macropomum to naphthalene injection and subsequent hypoxia exposure, emphasizing the expression of the tumor suppressor gene tp53. Tambaquis were intraperitoneally injected with naphthalene (50 mg/kg) and, after 96 hours, the fish were transferred to respirometry chambers and, submitted to progressive hypoxia for the determination of critical PO2. In a subsequent experiment, the fish received an intraperitoneal injection of naphthalene and were kept for 96 hours under normoxia. Successively, fish were challenged with acute hypoxia (PO2 <PO2 crit) during 6 hours. We observed that the PO2 crit was not affected by naphthalene injection. Moreover, hematological parameters were modulated only in response to hypoxia. Fish with naphthalene injection plus hypoxia exposure presented altered activity of the GST and CAT enzymes. Exposure to naphthalene also resulted in DNA damages, which was not influenced by hypoxia. Hypoxia accentuated the hepatic lesions caused by naphthalene, as well as it also impaired the transcription of tp53 in naphtalene injected fish, demonstrating the risks of contaminating aquatic environments, especially environments where hypoxic conditions are common and occur on a daily or on seasonal basis, as in the Amazon basin. © 2019, Sociedade Brasileira de Genética.Volume 42, Número 2, Pags. 411-424Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessCatalaseGlutathione TransferaseNaphthaleneAnimals ExperimentAnimals ModelAnimals TissueBiochemical AnalysisColossoma MacropomumControlled StudyDna DamageEcophysiologyGene ExpressionGenetic TranscriptionGenotoxicityHematological ParametersHistopathologyHypoxiaLipid PeroxidationLiver InjuryNonhumanOxygen TensionTumor Suppressor GeneEcophysiology, genotoxicity, histopathology, and gene responses of naphthalene injected colossoma macropomum (Cuvier, 1818) exposed to hypoxiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Biologyengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfapplication/pdf2287479https://repositorio.inpa.gov.br/bitstream/1/14963/1/artigo-inpa.pdf749334b64e8dc9ab820c4dac529a97e7MD51CC-LICENSElicense_rdfapplication/octet-stream914https://repositorio.inpa.gov.br/bitstream/1/14963/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD521/149632020-07-14 10:30:02.399oai:repositorio:1/14963Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T14:30:02Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Ecophysiology, genotoxicity, histopathology, and gene responses of naphthalene injected colossoma macropomum (Cuvier, 1818) exposed to hypoxia |
title |
Ecophysiology, genotoxicity, histopathology, and gene responses of naphthalene injected colossoma macropomum (Cuvier, 1818) exposed to hypoxia |
spellingShingle |
Ecophysiology, genotoxicity, histopathology, and gene responses of naphthalene injected colossoma macropomum (Cuvier, 1818) exposed to hypoxia Souza, Samara Silva de Catalase Glutathione Transferase Naphthalene Animals Experiment Animals Model Animals Tissue Biochemical Analysis Colossoma Macropomum Controlled Study Dna Damage Ecophysiology Gene Expression Genetic Transcription Genotoxicity Hematological Parameters Histopathology Hypoxia Lipid Peroxidation Liver Injury Nonhuman Oxygen Tension Tumor Suppressor Gene |
title_short |
Ecophysiology, genotoxicity, histopathology, and gene responses of naphthalene injected colossoma macropomum (Cuvier, 1818) exposed to hypoxia |
title_full |
Ecophysiology, genotoxicity, histopathology, and gene responses of naphthalene injected colossoma macropomum (Cuvier, 1818) exposed to hypoxia |
title_fullStr |
Ecophysiology, genotoxicity, histopathology, and gene responses of naphthalene injected colossoma macropomum (Cuvier, 1818) exposed to hypoxia |
title_full_unstemmed |
Ecophysiology, genotoxicity, histopathology, and gene responses of naphthalene injected colossoma macropomum (Cuvier, 1818) exposed to hypoxia |
title_sort |
Ecophysiology, genotoxicity, histopathology, and gene responses of naphthalene injected colossoma macropomum (Cuvier, 1818) exposed to hypoxia |
author |
Souza, Samara Silva de |
author_facet |
Souza, Samara Silva de Silva, Grazyelle Sebrenski da Almeida-Val, Vera Maria Fonseca de |
author_role |
author |
author2 |
Silva, Grazyelle Sebrenski da Almeida-Val, Vera Maria Fonseca de |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Souza, Samara Silva de Silva, Grazyelle Sebrenski da Almeida-Val, Vera Maria Fonseca de |
dc.subject.eng.fl_str_mv |
Catalase Glutathione Transferase Naphthalene Animals Experiment Animals Model Animals Tissue Biochemical Analysis Colossoma Macropomum Controlled Study Dna Damage Ecophysiology Gene Expression Genetic Transcription Genotoxicity Hematological Parameters Histopathology Hypoxia Lipid Peroxidation Liver Injury Nonhuman Oxygen Tension Tumor Suppressor Gene |
topic |
Catalase Glutathione Transferase Naphthalene Animals Experiment Animals Model Animals Tissue Biochemical Analysis Colossoma Macropomum Controlled Study Dna Damage Ecophysiology Gene Expression Genetic Transcription Genotoxicity Hematological Parameters Histopathology Hypoxia Lipid Peroxidation Liver Injury Nonhuman Oxygen Tension Tumor Suppressor Gene |
description |
The present study aimed to evaluate the biological responses of Colossoma macropomum to naphthalene injection and subsequent hypoxia exposure, emphasizing the expression of the tumor suppressor gene tp53. Tambaquis were intraperitoneally injected with naphthalene (50 mg/kg) and, after 96 hours, the fish were transferred to respirometry chambers and, submitted to progressive hypoxia for the determination of critical PO2. In a subsequent experiment, the fish received an intraperitoneal injection of naphthalene and were kept for 96 hours under normoxia. Successively, fish were challenged with acute hypoxia (PO2 <PO2 crit) during 6 hours. We observed that the PO2 crit was not affected by naphthalene injection. Moreover, hematological parameters were modulated only in response to hypoxia. Fish with naphthalene injection plus hypoxia exposure presented altered activity of the GST and CAT enzymes. Exposure to naphthalene also resulted in DNA damages, which was not influenced by hypoxia. Hypoxia accentuated the hepatic lesions caused by naphthalene, as well as it also impaired the transcription of tp53 in naphtalene injected fish, demonstrating the risks of contaminating aquatic environments, especially environments where hypoxic conditions are common and occur on a daily or on seasonal basis, as in the Amazon basin. © 2019, Sociedade Brasileira de Genética. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019 |
dc.date.accessioned.fl_str_mv |
2020-05-07T13:59:10Z |
dc.date.available.fl_str_mv |
2020-05-07T13:59:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/14963 |
dc.identifier.doi.none.fl_str_mv |
10.1590/1678-4685-gmb-2018-0084 |
url |
https://repositorio.inpa.gov.br/handle/1/14963 |
identifier_str_mv |
10.1590/1678-4685-gmb-2018-0084 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 42, Número 2, Pags. 411-424 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Genetics and Molecular Biology |
publisher.none.fl_str_mv |
Genetics and Molecular Biology |
dc.source.none.fl_str_mv |
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