Identification of Colletotrichum isolates from Capsicum chinense in Amazon

• Autor(a) principal: Oliveira, C. V.S. de
• Data de Publicação: 2017
• Outros Autores: Matos, Kedma Silva, Albuquerque, Dulcinéia Martins C. de, Hanada, Rogério Eiji, Silva, Gilvan Ferreira da
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional do INPA
• Texto Completo: https://repositorio.inpa.gov.br/handle/1/15002
• topic: Actin; Glyceraldehyde 3 Phosphate Dehydrogenase; Molecular Marker; Superoxide Dismutase; Amazonas; Capsicum Chinense; Colletotrichum; Colletotrichum Brevisporum; Colletotrichum Scovillei; Colletotrichum Siamense; Controlled Study; Crop Production; Fungus Identification; Fungus Isolation; Inter Simple Sequence Repeat; Nonhuman; Nucleotide Sequence; Phylogeny; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Unindexed Sequence

Chili pepper (Capsicum chinense) is a great economic important culture on the State of Amazonas, and it represents, approximately, a production of 1.9 thousand tons per year. It is one of the hosts of Colletotrichum genus in the North region of Brazil. The aim of the study was to differentiate and to identify isolates of Colletotrichum collected from C. chinense in Amazon. Molecular characterization, using RFLP-PCR, ERIC-PCR and ISSR, was carried out initially for screening of morphologically similar isolates. Furthermore, phylogenetic analyses were performed using combined regions: Actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for the three isolates, INPA 2066, INPA 2286 and INPA 1858, plus superoxide dismutase (SOD2) for INPA 2066. We showed that the molecular markers were able to distinguish the isolates of Colletotrichum studied and these results were confirmed with the phylogenetic analyses, three different occurrences of Colletotrichum species (C. siamense, C. scovillei and C. brevisporum) causing anthracnose in C. chinense in the State of Amazonas. This study represents the first report of the species C. siamense and C. scovillei in this host in Brazil. © 2017 The Authors.