The monotony of transferrin and esterase electrophoretic patterns in pirarucu, Arapaima gigas (Schinz, 1822) from Santa Cruz Lake, Tefé River, Amazonas, Brazil

• Autor(a) principal: Teixeira, Aylton Saturnino
• Data de Publicação: 2008
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional do INPA
• Texto Completo: https://repositorio.inpa.gov.br/handle/1/15006
• topic: Esterases; Transferrin; Allele; Animals Tissue; Arapaima Gigas; Brasil; Freshwater Fish; Gel Electrophoresis; Gene Locus; Polymorphism, Genetic; Genotype; Homozygosity; Lake; Nonhuman; River; Arapaima Gigas

Starch gel electrophoresis was used for examining the transferrin gene locus (Tf) and two esterase gene loci (Est-1 and Est-D1) of a pirarucu (Arapaima gigas) population sample collected from Santa Cruz Lake, Tefé River, Amazonas, Brazil. The Tf locus was tentatively classified as being polymorphic, showing two double-banded patterns (Tf12 and Tf22) of the three theoretically expected ones (Tf11, Tf12 and Tf 22), presumably controlled by two co-dominant alleles, Tf1 and Tf2. The monotony detected in pirarucu Tf locus genotypes showing a very high proportion of the double-banded heterozygote pattern Tf 12 (95% of the sampled individuals) may indicate the possibility of their having come from representatives of the same brood begotten by a pair of fish, where a single-banded Tf11 homozygote pattern male would have crossed with a single-banded Tf22 homozygote pattern female, or vice versa. One zone of electrophoretic activity was detected in esterase, presumably controlled by a monomorphic Est-1 locus with the fixed allele Est-11 where all individuals showed the single-banded Est-111 homozygote pattern. Esterase-D also displayed one zone of electrophoretic activity, presumably controlled by a monomorphic Est-D1 locus with a fixed allele Est-D11 where all individuals revealed the single-banded Est-D1 11 genotype pattern. The monotony comprised by single-banded genotype patterns in both esterase systems tested may also indicate the possibility of the individuals from the sample examined having come from representatives of the same brood begotten by a pair of fish with both the male and female having the same genotypes. ©FUNPEC-RP.