Evolutionary insights of the zw sex chromosomes in snakes: A new chapter added by the amazonian puffing snakes of the genus spilotes
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/15517 |
Resumo: | Amazonian puffing snakes (Spilotes; Colubridae) are snakes widely distributed in the Neotropical region. However, chromosomal data are scarce in this group and, when available, are only limited to karyotype description using conventional staining. In this paper, we focused on the process of karyotype evolution and trends for sex chromosomes in two Amazonian Puffer Snakes (S. pulllatus and S. sulphureus). We performed an extensive karyotype characterization using conventional and molecular cytogenetic approaches. The karyotype of S. sulphureus (presented here for the first time) exhibits a 2n = 36, similar to that previously described in S. pullatus. Both species have highly differentiated ZZ/ZW sex chromosomes, where the W chromosome is highly heterochromatic in S. pullatus but euchromatic in S. sulphureus. Both W chromosomes are homologous between these species as revealed by cross-species comparative genomic hybridization, even with heterogeneous distributions of several repetitive sequences across their genomes, including on the Z and on the W chromosomes. Our study provides evidence that W chromosomes in these two species have shared ancestry. © 2019 by the authors. Licensee MDPI, Basel, Switzerland. |
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Viana, Patrik FerreiraEzaz, Tariq TariqCioffi, Marcelo de BelloAlmeida, Breno Jackson L.Feldberg, Eliana2020-05-14T16:32:37Z2020-05-14T16:32:37Z2019https://repositorio.inpa.gov.br/handle/1/1551710.3390/genes10040288Amazonian puffing snakes (Spilotes; Colubridae) are snakes widely distributed in the Neotropical region. However, chromosomal data are scarce in this group and, when available, are only limited to karyotype description using conventional staining. In this paper, we focused on the process of karyotype evolution and trends for sex chromosomes in two Amazonian Puffer Snakes (S. pulllatus and S. sulphureus). We performed an extensive karyotype characterization using conventional and molecular cytogenetic approaches. The karyotype of S. sulphureus (presented here for the first time) exhibits a 2n = 36, similar to that previously described in S. pullatus. Both species have highly differentiated ZZ/ZW sex chromosomes, where the W chromosome is highly heterochromatic in S. pullatus but euchromatic in S. sulphureus. Both W chromosomes are homologous between these species as revealed by cross-species comparative genomic hybridization, even with heterogeneous distributions of several repetitive sequences across their genomes, including on the Z and on the W chromosomes. Our study provides evidence that W chromosomes in these two species have shared ancestry. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.Volume 10, Número 4Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessChromosome PuffClinical Comparative Genomic HybridizationGenusHeterochromatinKaryotype EvolutionNonhumanSex ChromosomeSnakeEvolutionary insights of the zw sex chromosomes in snakes: A new chapter added by the amazonian puffing snakes of the genus spilotesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenesengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa2.pdfartigo-inpa2.pdfapplication/pdf5887134https://repositorio.inpa.gov.br/bitstream/1/15517/1/artigo-inpa2.pdfb0c680b4c13daf8e67b9157390103d7fMD511/155172020-05-27 17:27:34.825oai:repositorio:1/15517Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-05-27T21:27:34Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Evolutionary insights of the zw sex chromosomes in snakes: A new chapter added by the amazonian puffing snakes of the genus spilotes |
title |
Evolutionary insights of the zw sex chromosomes in snakes: A new chapter added by the amazonian puffing snakes of the genus spilotes |
spellingShingle |
Evolutionary insights of the zw sex chromosomes in snakes: A new chapter added by the amazonian puffing snakes of the genus spilotes Viana, Patrik Ferreira Chromosome Puff Clinical Comparative Genomic Hybridization Genus Heterochromatin Karyotype Evolution Nonhuman Sex Chromosome Snake |
title_short |
Evolutionary insights of the zw sex chromosomes in snakes: A new chapter added by the amazonian puffing snakes of the genus spilotes |
title_full |
Evolutionary insights of the zw sex chromosomes in snakes: A new chapter added by the amazonian puffing snakes of the genus spilotes |
title_fullStr |
Evolutionary insights of the zw sex chromosomes in snakes: A new chapter added by the amazonian puffing snakes of the genus spilotes |
title_full_unstemmed |
Evolutionary insights of the zw sex chromosomes in snakes: A new chapter added by the amazonian puffing snakes of the genus spilotes |
title_sort |
Evolutionary insights of the zw sex chromosomes in snakes: A new chapter added by the amazonian puffing snakes of the genus spilotes |
author |
Viana, Patrik Ferreira |
author_facet |
Viana, Patrik Ferreira Ezaz, Tariq Tariq Cioffi, Marcelo de Bello Almeida, Breno Jackson L. Feldberg, Eliana |
author_role |
author |
author2 |
Ezaz, Tariq Tariq Cioffi, Marcelo de Bello Almeida, Breno Jackson L. Feldberg, Eliana |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Viana, Patrik Ferreira Ezaz, Tariq Tariq Cioffi, Marcelo de Bello Almeida, Breno Jackson L. Feldberg, Eliana |
dc.subject.eng.fl_str_mv |
Chromosome Puff Clinical Comparative Genomic Hybridization Genus Heterochromatin Karyotype Evolution Nonhuman Sex Chromosome Snake |
topic |
Chromosome Puff Clinical Comparative Genomic Hybridization Genus Heterochromatin Karyotype Evolution Nonhuman Sex Chromosome Snake |
description |
Amazonian puffing snakes (Spilotes; Colubridae) are snakes widely distributed in the Neotropical region. However, chromosomal data are scarce in this group and, when available, are only limited to karyotype description using conventional staining. In this paper, we focused on the process of karyotype evolution and trends for sex chromosomes in two Amazonian Puffer Snakes (S. pulllatus and S. sulphureus). We performed an extensive karyotype characterization using conventional and molecular cytogenetic approaches. The karyotype of S. sulphureus (presented here for the first time) exhibits a 2n = 36, similar to that previously described in S. pullatus. Both species have highly differentiated ZZ/ZW sex chromosomes, where the W chromosome is highly heterochromatic in S. pullatus but euchromatic in S. sulphureus. Both W chromosomes are homologous between these species as revealed by cross-species comparative genomic hybridization, even with heterogeneous distributions of several repetitive sequences across their genomes, including on the Z and on the W chromosomes. Our study provides evidence that W chromosomes in these two species have shared ancestry. © 2019 by the authors. Licensee MDPI, Basel, Switzerland. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019 |
dc.date.accessioned.fl_str_mv |
2020-05-14T16:32:37Z |
dc.date.available.fl_str_mv |
2020-05-14T16:32:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/15517 |
dc.identifier.doi.none.fl_str_mv |
10.3390/genes10040288 |
url |
https://repositorio.inpa.gov.br/handle/1/15517 |
identifier_str_mv |
10.3390/genes10040288 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 10, Número 4 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Genes |
publisher.none.fl_str_mv |
Genes |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional do INPA instname:Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
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Instituto Nacional de Pesquisas da Amazônia (INPA) |
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INPA |
institution |
INPA |
reponame_str |
Repositório Institucional do INPA |
collection |
Repositório Institucional do INPA |
bitstream.url.fl_str_mv |
https://repositorio.inpa.gov.br/bitstream/1/15517/1/artigo-inpa2.pdf |
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b0c680b4c13daf8e67b9157390103d7f |
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Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA) |
repository.mail.fl_str_mv |
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