Development and validation of analytical methodology by spectrophotometry in the visible for quantification of total tannins in the stem bark of Simarouba amara Aubl
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/15190 |
Resumo: | The stem bark of Simarouba amara Aubl., Simaroubaceae, is widely used in Brazilian folk medicine and in many countries of Central and South America for the treatment of malaria, intestinal parasites, diarrhea, anemia and fever. Although quassinoids are considered chemotaxonomic markers of the species, the unavailability of commercialization standards stay as a challenge for its quality control. Therefore, the purpose of this study was the development and validation of an analytical methodology by spectrophotometry to quantify the total tannin content from stem bark of S. amara, since this class of metabolites is one of the most abundant and representative in the species. The proposed method was based on quantification at 760 nm after addition of Folin-Ciocalteu reagent and its validation included all parameters recommended by the RE 899. The results showed that the proposed method was adequately developed and validated, and it is an useful analytical tool for the quality control of S. amara. © 2015, Sociedade de Investigacoes Florestais. All rights reserved. |
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Alves, Iasmine Andreza Basilio dos SantosMiranda, Henrique MarcelinoBarbosa, Antenor PereiraRandau, Karina PerrelliSoares, L. A.L.2020-05-07T14:09:25Z2020-05-07T14:09:25Z2015https://repositorio.inpa.gov.br/handle/1/1519010.1590/0100-67622015000100004The stem bark of Simarouba amara Aubl., Simaroubaceae, is widely used in Brazilian folk medicine and in many countries of Central and South America for the treatment of malaria, intestinal parasites, diarrhea, anemia and fever. Although quassinoids are considered chemotaxonomic markers of the species, the unavailability of commercialization standards stay as a challenge for its quality control. Therefore, the purpose of this study was the development and validation of an analytical methodology by spectrophotometry to quantify the total tannin content from stem bark of S. amara, since this class of metabolites is one of the most abundant and representative in the species. The proposed method was based on quantification at 760 nm after addition of Folin-Ciocalteu reagent and its validation included all parameters recommended by the RE 899. The results showed that the proposed method was adequately developed and validated, and it is an useful analytical tool for the quality control of S. amara. © 2015, Sociedade de Investigacoes Florestais. All rights reserved.Volume 39, Número 1, Pags. 37-47Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessDevelopment and validation of analytical methodology by spectrophotometry in the visible for quantification of total tannins in the stem bark of Simarouba amara AublDesenvolvimento e validacáo de metodologia analitica por espectrofotometria no visÍvel para quantificacäo de taninos totais na casca do caule de Simarouba amara Aublinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista Árvoreporreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfapplication/pdf939360https://repositorio.inpa.gov.br/bitstream/1/15190/1/artigo-inpa.pdf5f3d46d832e47315fa1723b7864fc13fMD51CC-LICENSElicense_rdfapplication/octet-stream914https://repositorio.inpa.gov.br/bitstream/1/15190/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD521/151902020-07-14 10:56:48.751oai:repositorio:1/15190Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T14:56:48Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.pt_BR.fl_str_mv |
Development and validation of analytical methodology by spectrophotometry in the visible for quantification of total tannins in the stem bark of Simarouba amara Aubl |
dc.title.alternative.pt_BR.fl_str_mv |
Desenvolvimento e validacáo de metodologia analitica por espectrofotometria no visÍvel para quantificacäo de taninos totais na casca do caule de Simarouba amara Aubl |
title |
Development and validation of analytical methodology by spectrophotometry in the visible for quantification of total tannins in the stem bark of Simarouba amara Aubl |
spellingShingle |
Development and validation of analytical methodology by spectrophotometry in the visible for quantification of total tannins in the stem bark of Simarouba amara Aubl Alves, Iasmine Andreza Basilio dos Santos |
title_short |
Development and validation of analytical methodology by spectrophotometry in the visible for quantification of total tannins in the stem bark of Simarouba amara Aubl |
title_full |
Development and validation of analytical methodology by spectrophotometry in the visible for quantification of total tannins in the stem bark of Simarouba amara Aubl |
title_fullStr |
Development and validation of analytical methodology by spectrophotometry in the visible for quantification of total tannins in the stem bark of Simarouba amara Aubl |
title_full_unstemmed |
Development and validation of analytical methodology by spectrophotometry in the visible for quantification of total tannins in the stem bark of Simarouba amara Aubl |
title_sort |
Development and validation of analytical methodology by spectrophotometry in the visible for quantification of total tannins in the stem bark of Simarouba amara Aubl |
author |
Alves, Iasmine Andreza Basilio dos Santos |
author_facet |
Alves, Iasmine Andreza Basilio dos Santos Miranda, Henrique Marcelino Barbosa, Antenor Pereira Randau, Karina Perrelli Soares, L. A.L. |
author_role |
author |
author2 |
Miranda, Henrique Marcelino Barbosa, Antenor Pereira Randau, Karina Perrelli Soares, L. A.L. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Alves, Iasmine Andreza Basilio dos Santos Miranda, Henrique Marcelino Barbosa, Antenor Pereira Randau, Karina Perrelli Soares, L. A.L. |
description |
The stem bark of Simarouba amara Aubl., Simaroubaceae, is widely used in Brazilian folk medicine and in many countries of Central and South America for the treatment of malaria, intestinal parasites, diarrhea, anemia and fever. Although quassinoids are considered chemotaxonomic markers of the species, the unavailability of commercialization standards stay as a challenge for its quality control. Therefore, the purpose of this study was the development and validation of an analytical methodology by spectrophotometry to quantify the total tannin content from stem bark of S. amara, since this class of metabolites is one of the most abundant and representative in the species. The proposed method was based on quantification at 760 nm after addition of Folin-Ciocalteu reagent and its validation included all parameters recommended by the RE 899. The results showed that the proposed method was adequately developed and validated, and it is an useful analytical tool for the quality control of S. amara. © 2015, Sociedade de Investigacoes Florestais. All rights reserved. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015 |
dc.date.accessioned.fl_str_mv |
2020-05-07T14:09:25Z |
dc.date.available.fl_str_mv |
2020-05-07T14:09:25Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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https://repositorio.inpa.gov.br/handle/1/15190 |
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10.1590/0100-67622015000100004 |
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https://repositorio.inpa.gov.br/handle/1/15190 |
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10.1590/0100-67622015000100004 |
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por |
language |
por |
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Volume 39, Número 1, Pags. 37-47 |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
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openAccess |
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Revista Árvore |
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Revista Árvore |
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