Larvicidal effects of endophytic and basidiomycete fungus extracts on Aedes and Anopheles larvae (Diptera, Culicidae)

• Autor(a) principal: Bücker, Augusto
• Data de Publicação: 2013
• Outros Autores: Bücker, Nádia Cristina Falcão, Souza, Antônia Queiroz Lima de, Gama, Auricélia Matos da, Rodrigues-Filho, Edson, Costa, Fábio Medeiros da, Nunez, C. V., Silva, Ademir Castro, Tadei, Wanderli Pedro
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional do INPA
• Texto Completo: https://repositorio.inpa.gov.br/handle/1/15150
• topic: Acetic Acid Ethyl Ester; Fungal Extract; Aedes Aegypti; Anopheles; Anopheles Nuneztovari; Arthropod Larva; Basidiomycetes; Controlled Study; Endophyte; Female; Fungus; Fungus Culture; In Vitro Study; Insecticidal Activity; Mycelium; Nonhuman; Pestalotiopsis Virgulata; Pycnoporus Sanguineus; Aedes; Animals; Anopheles; Basidiomycetes; Bioassay; Chemistry; Disease Carrier; Drug Effects; Larva; Ld 50; Aedes Aegypti; Amphisphaeriaceae; Anopheles Nuneztovari; Basidiomycota; Culicidae; Diptera; Fungi; Melanconiales; Pestalotiopsis; Polyporaceae; Pycnoporus Sanguineus; Aedes; Animal; Anopheles; Basidiomycota; Biological Assay; Endophytes; Insect Vectors; Larva; Lethal Dose 50

Introduction: In vitro bioassays were performed to access the larvicidal activity of crude extracts from the endophytic fungus Pestalotiopsis virgulata (Melanconiales, Amphisphaeriaceae) and the saprophytic fungus Pycnoporus sanguineus (Basidiomycetes, Polyporaceae) against the mosquitoes Aedes aegypti and Anopheles nuneztovari. Methods: The extracts were tested at concentrations of 100, 200, 300, 400 and 500ppm. Ethyl acetate mycelia (EAM) extracts and liquid culture media (LCM) from Pe. virgulata and Py. sanguineus were tested against third instar larvae of Ae. aegypti and An. nuneztovari. Results: The larvicidal activity of the EAM extracts from Pe. virgulata against Ae. aegypti had an LC50=101.8ppm, and the extract from the basidiomycete fungus Py. sanguineus had an LC50=156.8ppm against the Ae. aegypti larvae. The Pe. virgulata extract had an LC50=16.3ppm against the An. nuneztovari larvae, and the Py. sanguineus extract had an LC50=87.2ppm against these larvae. Conclusions: These results highlight the larvicidal effect of EAM extracts from the endophyte Pe. virgulata against the two larval mosquitoes tested. Thus, Pe. virgulata and Py. sanguineus have the potential for the production of bioactive substances against larvae of these two tropical disease vectors, with An. nuneztovari being more susceptible to these extracts.