Evaluation of the taxonomic status of populations assigned to Phyllomedusa hypochondrialis (Anura, Hylidae, Phyllomedusinae) based on molecular, chromosomal, and morphological approach
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
DOI: | 10.1186/1471-2156-14-70 |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/16044 |
Resumo: | Background: The taxonomic and phylogenetic relationships of the genus Phyllomedusa have been amply discussed. The marked morphological similarities among some species hamper the reliable identification of specimens and may often lead to their incorrect taxonomic classification on the sole basis of morphological traits. Phenotypic variation was observed among populations assigned to either P. azurea or P. hypochondrialis. In order to evaluate whether the variation observed in populations assigned to P. hypochondrialis is related to that in genotypes, a cytogenetic analysis was combined with phylogenetic inferences based on mitochondrial and nuclear sequences.Results: The inter- and intra-population variation in the external morphology observed among the specimens analyzed in the present study do not reflect the phylogenetic relationships among populations. A monophyletic clade was recovered, grouping all the specimens identified as P. hypochondrialis and specimens assigned P. azurea from Minas Gerais state. This clade is characterized by conserved chromosomal morphology and a common C-banding pattern. Extensive variation in the nucleolar organizing region (NOR) was observed among populations, with four distinct NOR positions being recognized in the karyotypes. Intra-population polymorphism of the additional rDNA clusters observed in specimens from Barreiras, Bahia state, also highlights the marked genomic instability of the rDNA in the genome of this group. Based on the topology obtained in the phylogenetic analyses, the re-evaluation of the taxonomic status of the specimens from the southernmost population known in Brazil is recommended.Conclusions: The results of this study support the need for a thorough revision of the phenotypic features used to discriminate P. azurea and P. hypochondrialis. The phylogenetic data presented here also contribute to an extension of the geographic range of P. hypochondrialis, which is known to occur in the Amazon basin and neighboring areas of the Cerrado savanna, where it may be sympatric with P. azurea, within contact zones. The misidentification of specimens may have led to inconsistencies in the original definition of the geographic range of P. azurea. The variability observed in the NOR of P. hypochondrialis reinforces the conclusion that these sites represent hotspots of rearrangement. Intraspecific variation in the location of these sites is the result of constant rearrangements that are not detected by classical cytogenetic methods or are traits of an ancestral, polymorphic karyotype, which would not be phylogenetically informative for this group. © 2013 Bruschi et al.; licensee BioMed Central Ltd. |
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Bruschi, Daniel PachecoBusin, Carmen SílviaFelipe, Toledo, LuísVasconcellos, Gilda A.Strüssmann, ChristineWeber, Luiz NorbertoLima, Albertina PimentalLima, Jucivaldo DiasPimentel, Shirlei Maria Recco2020-05-22T18:37:53Z2020-05-22T18:37:53Z2013https://repositorio.inpa.gov.br/handle/1/1604410.1186/1471-2156-14-70Background: The taxonomic and phylogenetic relationships of the genus Phyllomedusa have been amply discussed. The marked morphological similarities among some species hamper the reliable identification of specimens and may often lead to their incorrect taxonomic classification on the sole basis of morphological traits. Phenotypic variation was observed among populations assigned to either P. azurea or P. hypochondrialis. In order to evaluate whether the variation observed in populations assigned to P. hypochondrialis is related to that in genotypes, a cytogenetic analysis was combined with phylogenetic inferences based on mitochondrial and nuclear sequences.Results: The inter- and intra-population variation in the external morphology observed among the specimens analyzed in the present study do not reflect the phylogenetic relationships among populations. A monophyletic clade was recovered, grouping all the specimens identified as P. hypochondrialis and specimens assigned P. azurea from Minas Gerais state. This clade is characterized by conserved chromosomal morphology and a common C-banding pattern. Extensive variation in the nucleolar organizing region (NOR) was observed among populations, with four distinct NOR positions being recognized in the karyotypes. Intra-population polymorphism of the additional rDNA clusters observed in specimens from Barreiras, Bahia state, also highlights the marked genomic instability of the rDNA in the genome of this group. Based on the topology obtained in the phylogenetic analyses, the re-evaluation of the taxonomic status of the specimens from the southernmost population known in Brazil is recommended.Conclusions: The results of this study support the need for a thorough revision of the phenotypic features used to discriminate P. azurea and P. hypochondrialis. The phylogenetic data presented here also contribute to an extension of the geographic range of P. hypochondrialis, which is known to occur in the Amazon basin and neighboring areas of the Cerrado savanna, where it may be sympatric with P. azurea, within contact zones. The misidentification of specimens may have led to inconsistencies in the original definition of the geographic range of P. azurea. The variability observed in the NOR of P. hypochondrialis reinforces the conclusion that these sites represent hotspots of rearrangement. Intraspecific variation in the location of these sites is the result of constant rearrangements that are not detected by classical cytogenetic methods or are traits of an ancestral, polymorphic karyotype, which would not be phylogenetically informative for this group. © 2013 Bruschi et al.; licensee BioMed Central Ltd.Volume 14, Pags. 1-14Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessRibosome DnaAnimals ExperimentBrasilC BandingChromosome AnalysisChromosome NorCladisticsConservation GeneticsControlled StudyCytogeneticsGene ClusterGenetic VariabilityGenomeGenomic InstabilityGenotypeIntraspecific VariationKaryotypeMaleMorphological TraitNonhumanPhyllomedusaPhyllomedusa HypochondrialisPhylogenyGenetics, PopulationPolymorphism, Single NucleotideTaxonomic IdentificationTaxonomyAnimalsAnuraChromosomeClassificationGeneticsKaryotypingPhylogenyAnuraHylidaePhyllomedusaPhyllomedusa HypochondrialisPhyllomedusinaeAnimalAnuraChromosomesKaryotypingNucleolus Organizer RegionPhylogenyEvaluation of the taxonomic status of populations assigned to Phyllomedusa hypochondrialis (Anura, Hylidae, Phyllomedusinae) based on molecular, chromosomal, and morphological approachinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBMC Geneticsengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa7.pdfartigo-inpa7.pdfapplication/pdf1453601https://repositorio.inpa.gov.br/bitstream/1/16044/1/artigo-inpa7.pdfb1379df697b3bf2654872e93fb3d63d2MD511/160442020-07-14 11:28:09.504oai:repositorio:1/16044Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T15:28:09Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Evaluation of the taxonomic status of populations assigned to Phyllomedusa hypochondrialis (Anura, Hylidae, Phyllomedusinae) based on molecular, chromosomal, and morphological approach |
title |
Evaluation of the taxonomic status of populations assigned to Phyllomedusa hypochondrialis (Anura, Hylidae, Phyllomedusinae) based on molecular, chromosomal, and morphological approach |
spellingShingle |
Evaluation of the taxonomic status of populations assigned to Phyllomedusa hypochondrialis (Anura, Hylidae, Phyllomedusinae) based on molecular, chromosomal, and morphological approach Bruschi, Daniel Pacheco Ribosome Dna Animals Experiment Brasil C Banding Chromosome Analysis Chromosome Nor Cladistics Conservation Genetics Controlled Study Cytogenetics Gene Cluster Genetic Variability Genome Genomic Instability Genotype Intraspecific Variation Karyotype Male Morphological Trait Nonhuman Phyllomedusa Phyllomedusa Hypochondrialis Phylogeny Genetics, Population Polymorphism, Single Nucleotide Taxonomic Identification Taxonomy Animals Anura Chromosome Classification Genetics Karyotyping Phylogeny Anura Hylidae Phyllomedusa Phyllomedusa Hypochondrialis Phyllomedusinae Animal Anura Chromosomes Karyotyping Nucleolus Organizer Region Phylogeny |
title_short |
Evaluation of the taxonomic status of populations assigned to Phyllomedusa hypochondrialis (Anura, Hylidae, Phyllomedusinae) based on molecular, chromosomal, and morphological approach |
title_full |
Evaluation of the taxonomic status of populations assigned to Phyllomedusa hypochondrialis (Anura, Hylidae, Phyllomedusinae) based on molecular, chromosomal, and morphological approach |
title_fullStr |
Evaluation of the taxonomic status of populations assigned to Phyllomedusa hypochondrialis (Anura, Hylidae, Phyllomedusinae) based on molecular, chromosomal, and morphological approach |
title_full_unstemmed |
Evaluation of the taxonomic status of populations assigned to Phyllomedusa hypochondrialis (Anura, Hylidae, Phyllomedusinae) based on molecular, chromosomal, and morphological approach |
title_sort |
Evaluation of the taxonomic status of populations assigned to Phyllomedusa hypochondrialis (Anura, Hylidae, Phyllomedusinae) based on molecular, chromosomal, and morphological approach |
author |
Bruschi, Daniel Pacheco |
author_facet |
Bruschi, Daniel Pacheco Busin, Carmen Sílvia Felipe, Toledo, Luís Vasconcellos, Gilda A. Strüssmann, Christine Weber, Luiz Norberto Lima, Albertina Pimental Lima, Jucivaldo Dias Pimentel, Shirlei Maria Recco |
author_role |
author |
author2 |
Busin, Carmen Sílvia Felipe, Toledo, Luís Vasconcellos, Gilda A. Strüssmann, Christine Weber, Luiz Norberto Lima, Albertina Pimental Lima, Jucivaldo Dias Pimentel, Shirlei Maria Recco |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Bruschi, Daniel Pacheco Busin, Carmen Sílvia Felipe, Toledo, Luís Vasconcellos, Gilda A. Strüssmann, Christine Weber, Luiz Norberto Lima, Albertina Pimental Lima, Jucivaldo Dias Pimentel, Shirlei Maria Recco |
dc.subject.eng.fl_str_mv |
Ribosome Dna Animals Experiment Brasil C Banding Chromosome Analysis Chromosome Nor Cladistics Conservation Genetics Controlled Study Cytogenetics Gene Cluster Genetic Variability Genome Genomic Instability Genotype Intraspecific Variation Karyotype Male Morphological Trait Nonhuman Phyllomedusa Phyllomedusa Hypochondrialis Phylogeny Genetics, Population Polymorphism, Single Nucleotide Taxonomic Identification Taxonomy Animals Anura Chromosome Classification Genetics Karyotyping Phylogeny Anura Hylidae Phyllomedusa Phyllomedusa Hypochondrialis Phyllomedusinae Animal Anura Chromosomes Karyotyping Nucleolus Organizer Region Phylogeny |
topic |
Ribosome Dna Animals Experiment Brasil C Banding Chromosome Analysis Chromosome Nor Cladistics Conservation Genetics Controlled Study Cytogenetics Gene Cluster Genetic Variability Genome Genomic Instability Genotype Intraspecific Variation Karyotype Male Morphological Trait Nonhuman Phyllomedusa Phyllomedusa Hypochondrialis Phylogeny Genetics, Population Polymorphism, Single Nucleotide Taxonomic Identification Taxonomy Animals Anura Chromosome Classification Genetics Karyotyping Phylogeny Anura Hylidae Phyllomedusa Phyllomedusa Hypochondrialis Phyllomedusinae Animal Anura Chromosomes Karyotyping Nucleolus Organizer Region Phylogeny |
description |
Background: The taxonomic and phylogenetic relationships of the genus Phyllomedusa have been amply discussed. The marked morphological similarities among some species hamper the reliable identification of specimens and may often lead to their incorrect taxonomic classification on the sole basis of morphological traits. Phenotypic variation was observed among populations assigned to either P. azurea or P. hypochondrialis. In order to evaluate whether the variation observed in populations assigned to P. hypochondrialis is related to that in genotypes, a cytogenetic analysis was combined with phylogenetic inferences based on mitochondrial and nuclear sequences.Results: The inter- and intra-population variation in the external morphology observed among the specimens analyzed in the present study do not reflect the phylogenetic relationships among populations. A monophyletic clade was recovered, grouping all the specimens identified as P. hypochondrialis and specimens assigned P. azurea from Minas Gerais state. This clade is characterized by conserved chromosomal morphology and a common C-banding pattern. Extensive variation in the nucleolar organizing region (NOR) was observed among populations, with four distinct NOR positions being recognized in the karyotypes. Intra-population polymorphism of the additional rDNA clusters observed in specimens from Barreiras, Bahia state, also highlights the marked genomic instability of the rDNA in the genome of this group. Based on the topology obtained in the phylogenetic analyses, the re-evaluation of the taxonomic status of the specimens from the southernmost population known in Brazil is recommended.Conclusions: The results of this study support the need for a thorough revision of the phenotypic features used to discriminate P. azurea and P. hypochondrialis. The phylogenetic data presented here also contribute to an extension of the geographic range of P. hypochondrialis, which is known to occur in the Amazon basin and neighboring areas of the Cerrado savanna, where it may be sympatric with P. azurea, within contact zones. The misidentification of specimens may have led to inconsistencies in the original definition of the geographic range of P. azurea. The variability observed in the NOR of P. hypochondrialis reinforces the conclusion that these sites represent hotspots of rearrangement. Intraspecific variation in the location of these sites is the result of constant rearrangements that are not detected by classical cytogenetic methods or are traits of an ancestral, polymorphic karyotype, which would not be phylogenetically informative for this group. © 2013 Bruschi et al.; licensee BioMed Central Ltd. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
dc.date.accessioned.fl_str_mv |
2020-05-22T18:37:53Z |
dc.date.available.fl_str_mv |
2020-05-22T18:37:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/16044 |
dc.identifier.doi.none.fl_str_mv |
10.1186/1471-2156-14-70 |
url |
https://repositorio.inpa.gov.br/handle/1/16044 |
identifier_str_mv |
10.1186/1471-2156-14-70 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 14, Pags. 1-14 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
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openAccess |
dc.publisher.none.fl_str_mv |
BMC Genetics |
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BMC Genetics |
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INPA |
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Repositório Institucional do INPA |
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Repositório Institucional do INPA |
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