Molecular taxonomy and evolutionary relationships in the Oswaldoi-Konderi complex (Anophelinae: Anopheles: Nyssorhynchus) from the Brazilian Amazon region

Detalhes bibliográficos
Autor(a) principal: Saraiva, José Ferreira
Data de Publicação: 2018
Outros Autores: Picanço Souto, Raimundo Nonato, Scarpassa, Vera Margarete
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional do INPA
Texto Completo: https://repositorio.inpa.gov.br/handle/1/14662
Resumo: Recent studies have shown that Anopheles oswaldoi sensu lato comprises a cryptic species complex in South America. Anopheles konderi, which was previously raised to synonymy with An. oswaldoi, has also been suggested to form a species complex. An. oswaldoi has been incriminated as a malaria vector in some areas of the Brazilian Amazon, Colombia, Peru and Venezuela, but was not recognized as a vector in the remaining regions in its geographic distribution. The role of An. konderi as a malaria vector is unknown or has been misattributed to An. oswaldoi. The focus of this study was molecular identification to infer the evolutionary relationships and preliminarily delimit the geographic distribution of the members of these complexes in the Brazilian Amazon region. The specimens were sampled from 18 localities belonging to five states in the Brazilian Amazon and sequenced for two molecular markers: the DNA barcode region (COI gene of mitochondrial DNA) and Internal Transcribed Spacer 2 (ITS2 ribosomal DNA). COI (83 sequences) and ITS2 (27 sequences) datasets generated 43 and 10 haplotypes, respectively. Haplotype networks and phylogenetic analyses generated with the barcode region (COI gene) recovered five groups corresponding to An. oswaldoi s.s., An. oswaldoi B, An. oswaldoi A, An. konderi and An. sp. nr. konderi; all pairwise genetic distances were greater than 3%. The group represented by An. oswaldoi A exhibited three strongly supported lineages. The molecular dating indicated that the diversification process in these complexes started approximately 2.8 Mya, in the Pliocene. These findings confirm five very closely related species and present new records for these species in the Brazilian Amazon region. The paraphyly observed for the An. oswaldoi complex suggests that An. oswaldoi and An. konderi complexes may comprise a unique species complex named Oswaldoi-Konderi. Anopheles oswaldoi B may be a potential malaria vector in the extreme north of the Brazilian Amazon, whereas evidence of sympatry for the remaining species in other parts of the Brazilian Amazon (Acre, Amazonas, Pará and Rondônia) precluded identification of probable vectors in those areas. © 2018 Saraiva et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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spelling Saraiva, José FerreiraPicanço Souto, Raimundo NonatoScarpassa, Vera Margarete2020-04-24T17:00:01Z2020-04-24T17:00:01Z2018https://repositorio.inpa.gov.br/handle/1/1466210.1371/journal.pone.0193591Recent studies have shown that Anopheles oswaldoi sensu lato comprises a cryptic species complex in South America. Anopheles konderi, which was previously raised to synonymy with An. oswaldoi, has also been suggested to form a species complex. An. oswaldoi has been incriminated as a malaria vector in some areas of the Brazilian Amazon, Colombia, Peru and Venezuela, but was not recognized as a vector in the remaining regions in its geographic distribution. The role of An. konderi as a malaria vector is unknown or has been misattributed to An. oswaldoi. The focus of this study was molecular identification to infer the evolutionary relationships and preliminarily delimit the geographic distribution of the members of these complexes in the Brazilian Amazon region. The specimens were sampled from 18 localities belonging to five states in the Brazilian Amazon and sequenced for two molecular markers: the DNA barcode region (COI gene of mitochondrial DNA) and Internal Transcribed Spacer 2 (ITS2 ribosomal DNA). COI (83 sequences) and ITS2 (27 sequences) datasets generated 43 and 10 haplotypes, respectively. Haplotype networks and phylogenetic analyses generated with the barcode region (COI gene) recovered five groups corresponding to An. oswaldoi s.s., An. oswaldoi B, An. oswaldoi A, An. konderi and An. sp. nr. konderi; all pairwise genetic distances were greater than 3%. The group represented by An. oswaldoi A exhibited three strongly supported lineages. The molecular dating indicated that the diversification process in these complexes started approximately 2.8 Mya, in the Pliocene. These findings confirm five very closely related species and present new records for these species in the Brazilian Amazon region. The paraphyly observed for the An. oswaldoi complex suggests that An. oswaldoi and An. konderi complexes may comprise a unique species complex named Oswaldoi-Konderi. Anopheles oswaldoi B may be a potential malaria vector in the extreme north of the Brazilian Amazon, whereas evidence of sympatry for the remaining species in other parts of the Brazilian Amazon (Acre, Amazonas, Pará and Rondônia) precluded identification of probable vectors in those areas. © 2018 Saraiva et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Volume 13, Número 3Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessInternal Transcribed Spacer 2Dna, MitochondrialDna, MitochondrialRibosome DnaSpacer DnaAdultAnimals TissueAnophelesAnopheles KonderiAnopheles OswaldoiBrasilCoi GeneControlled StudyDna BarcodingEvolutionFemaleGeneGenetic DistanceGeographic DistributionHaplotypeNonhumanParaphylyPhylogenyTaxonomyAnimalsAnophelesClassificationGeneticsEvolution, MolecularAnimalssAnophelesBrasilDna Barcoding, TaxonomicDna, IntergenicDna, MitochondrialRibosomal DnaEvolution, MolecularPhylogenyMolecular taxonomy and evolutionary relationships in the Oswaldoi-Konderi complex (Anophelinae: Anopheles: Nyssorhynchus) from the Brazilian Amazon regioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePLoS ONEengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfapplication/pdf8057653https://repositorio.inpa.gov.br/bitstream/1/14662/1/artigo-inpa.pdf54037d120e7a20b3bd4591f47b2b7265MD51CC-LICENSElicense_rdfapplication/octet-stream914https://repositorio.inpa.gov.br/bitstream/1/14662/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD521/146622020-07-14 09:19:20.636oai:repositorio:1/14662Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T13:19:20Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.en.fl_str_mv Molecular taxonomy and evolutionary relationships in the Oswaldoi-Konderi complex (Anophelinae: Anopheles: Nyssorhynchus) from the Brazilian Amazon region
title Molecular taxonomy and evolutionary relationships in the Oswaldoi-Konderi complex (Anophelinae: Anopheles: Nyssorhynchus) from the Brazilian Amazon region
spellingShingle Molecular taxonomy and evolutionary relationships in the Oswaldoi-Konderi complex (Anophelinae: Anopheles: Nyssorhynchus) from the Brazilian Amazon region
Saraiva, José Ferreira
Internal Transcribed Spacer 2
Dna, Mitochondrial
Dna, Mitochondrial
Ribosome Dna
Spacer Dna
Adult
Animals Tissue
Anopheles
Anopheles Konderi
Anopheles Oswaldoi
Brasil
Coi Gene
Controlled Study
Dna Barcoding
Evolution
Female
Gene
Genetic Distance
Geographic Distribution
Haplotype
Nonhuman
Paraphyly
Phylogeny
Taxonomy
Animals
Anopheles
Classification
Genetics
Evolution, Molecular
Animalss
Anopheles
Brasil
Dna Barcoding, Taxonomic
Dna, Intergenic
Dna, Mitochondrial
Ribosomal Dna
Evolution, Molecular
Phylogeny
title_short Molecular taxonomy and evolutionary relationships in the Oswaldoi-Konderi complex (Anophelinae: Anopheles: Nyssorhynchus) from the Brazilian Amazon region
title_full Molecular taxonomy and evolutionary relationships in the Oswaldoi-Konderi complex (Anophelinae: Anopheles: Nyssorhynchus) from the Brazilian Amazon region
title_fullStr Molecular taxonomy and evolutionary relationships in the Oswaldoi-Konderi complex (Anophelinae: Anopheles: Nyssorhynchus) from the Brazilian Amazon region
title_full_unstemmed Molecular taxonomy and evolutionary relationships in the Oswaldoi-Konderi complex (Anophelinae: Anopheles: Nyssorhynchus) from the Brazilian Amazon region
title_sort Molecular taxonomy and evolutionary relationships in the Oswaldoi-Konderi complex (Anophelinae: Anopheles: Nyssorhynchus) from the Brazilian Amazon region
author Saraiva, José Ferreira
author_facet Saraiva, José Ferreira
Picanço Souto, Raimundo Nonato
Scarpassa, Vera Margarete
author_role author
author2 Picanço Souto, Raimundo Nonato
Scarpassa, Vera Margarete
author2_role author
author
dc.contributor.author.fl_str_mv Saraiva, José Ferreira
Picanço Souto, Raimundo Nonato
Scarpassa, Vera Margarete
dc.subject.eng.fl_str_mv Internal Transcribed Spacer 2
Dna, Mitochondrial
Dna, Mitochondrial
Ribosome Dna
Spacer Dna
Adult
Animals Tissue
Anopheles
Anopheles Konderi
Anopheles Oswaldoi
Brasil
Coi Gene
Controlled Study
Dna Barcoding
Evolution
Female
Gene
Genetic Distance
Geographic Distribution
Haplotype
Nonhuman
Paraphyly
Phylogeny
Taxonomy
Animals
Anopheles
Classification
Genetics
Evolution, Molecular
Animalss
Anopheles
Brasil
Dna Barcoding, Taxonomic
Dna, Intergenic
Dna, Mitochondrial
Ribosomal Dna
Evolution, Molecular
Phylogeny
topic Internal Transcribed Spacer 2
Dna, Mitochondrial
Dna, Mitochondrial
Ribosome Dna
Spacer Dna
Adult
Animals Tissue
Anopheles
Anopheles Konderi
Anopheles Oswaldoi
Brasil
Coi Gene
Controlled Study
Dna Barcoding
Evolution
Female
Gene
Genetic Distance
Geographic Distribution
Haplotype
Nonhuman
Paraphyly
Phylogeny
Taxonomy
Animals
Anopheles
Classification
Genetics
Evolution, Molecular
Animalss
Anopheles
Brasil
Dna Barcoding, Taxonomic
Dna, Intergenic
Dna, Mitochondrial
Ribosomal Dna
Evolution, Molecular
Phylogeny
description Recent studies have shown that Anopheles oswaldoi sensu lato comprises a cryptic species complex in South America. Anopheles konderi, which was previously raised to synonymy with An. oswaldoi, has also been suggested to form a species complex. An. oswaldoi has been incriminated as a malaria vector in some areas of the Brazilian Amazon, Colombia, Peru and Venezuela, but was not recognized as a vector in the remaining regions in its geographic distribution. The role of An. konderi as a malaria vector is unknown or has been misattributed to An. oswaldoi. The focus of this study was molecular identification to infer the evolutionary relationships and preliminarily delimit the geographic distribution of the members of these complexes in the Brazilian Amazon region. The specimens were sampled from 18 localities belonging to five states in the Brazilian Amazon and sequenced for two molecular markers: the DNA barcode region (COI gene of mitochondrial DNA) and Internal Transcribed Spacer 2 (ITS2 ribosomal DNA). COI (83 sequences) and ITS2 (27 sequences) datasets generated 43 and 10 haplotypes, respectively. Haplotype networks and phylogenetic analyses generated with the barcode region (COI gene) recovered five groups corresponding to An. oswaldoi s.s., An. oswaldoi B, An. oswaldoi A, An. konderi and An. sp. nr. konderi; all pairwise genetic distances were greater than 3%. The group represented by An. oswaldoi A exhibited three strongly supported lineages. The molecular dating indicated that the diversification process in these complexes started approximately 2.8 Mya, in the Pliocene. These findings confirm five very closely related species and present new records for these species in the Brazilian Amazon region. The paraphyly observed for the An. oswaldoi complex suggests that An. oswaldoi and An. konderi complexes may comprise a unique species complex named Oswaldoi-Konderi. Anopheles oswaldoi B may be a potential malaria vector in the extreme north of the Brazilian Amazon, whereas evidence of sympatry for the remaining species in other parts of the Brazilian Amazon (Acre, Amazonas, Pará and Rondônia) precluded identification of probable vectors in those areas. © 2018 Saraiva et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2020-04-24T17:00:01Z
dc.date.available.fl_str_mv 2020-04-24T17:00:01Z
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dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0193591
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identifier_str_mv 10.1371/journal.pone.0193591
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dc.relation.ispartof.pt_BR.fl_str_mv Volume 13, Número 3
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http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
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