Partial purification of trypsin inhibitors from Caesalpinia ferrea and Swartzia polyphylla seeds and effect of protein extracts on pathogenic fungi

Detalhes bibliográficos
Autor(a) principal: Bariani, Adriana
Data de Publicação: 2012
Outros Autores: Gonçalves, José Francisco Carvalho de, Chevreuil, Larissa Ramos, Cavallazzi, José Renato Pereira, Souza, Luis Augusto Gomes de, Bentes, Jânia Lilia da Silva, Pando, Silvana Cristina
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional do INPA
Texto Completo: https://repositorio.inpa.gov.br/handle/1/16135
Resumo: Legume seeds present high concentration of trypsin inhibitors; these proteins are involved in both cell metabolism and plant defense mechanisms. In order to confirm or not a possible fungicidal action of these proteins from extracts of seeds of legume tree species, the aim this study was to detect trypsin inhibitors in seeds of Caesalpinia ferrea (CfTI) and Swartzia polyphylla (SpTI) and to test their extracts against the pathogenic fungi Colletotrichum guaranicola, Corynespora cassiicola, Fusarium oxysporum and Sclerotium rolfsii, evaluating mycelial growth and sporulation. Thus, samples of the plant material, finely powdered seeds, were extracted in 150 mM NaCl. The protein extracts were partially purified on Sephadex G-100 column, subjected to detection of inhibitors and SDS-PAGE (12.5%), and used in bioassays against the fungi. The electrophoretic profile revealed a single band in CfTI and eight bands in SPTI. The extracts of C. ferrea and S. polyphylla had an effect in reducing sporulation of the tested fungi, but S. rolfsii was inhibited only by C. ferrea. As to mycelial growth, the two extracts showed an effect on F. oxysporum and S. rolfsii, while C. guaranicola was inhibited only by S. polyphylla, and C. cassiicola by C. ferrea. It was concluded that seeds of C. ferrea and S. polyphylla present trypsin inhibitors. In addition, the results suggest that these tropical tree species are promising for the exploration of natural fungicides, since the extracts reduced mycelial growth and sporulation of C. guaranicola, C. cassiicola, F. oxysporum and S. rolfsii.
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spelling Bariani, AdrianaGonçalves, José Francisco Carvalho deChevreuil, Larissa RamosCavallazzi, José Renato PereiraSouza, Luis Augusto Gomes deBentes, Jânia Lilia da SilvaPando, Silvana Cristina2020-05-24T22:15:13Z2020-05-24T22:15:13Z2012https://repositorio.inpa.gov.br/handle/1/1613510.1590/S0100-54052012000200004Legume seeds present high concentration of trypsin inhibitors; these proteins are involved in both cell metabolism and plant defense mechanisms. In order to confirm or not a possible fungicidal action of these proteins from extracts of seeds of legume tree species, the aim this study was to detect trypsin inhibitors in seeds of Caesalpinia ferrea (CfTI) and Swartzia polyphylla (SpTI) and to test their extracts against the pathogenic fungi Colletotrichum guaranicola, Corynespora cassiicola, Fusarium oxysporum and Sclerotium rolfsii, evaluating mycelial growth and sporulation. Thus, samples of the plant material, finely powdered seeds, were extracted in 150 mM NaCl. The protein extracts were partially purified on Sephadex G-100 column, subjected to detection of inhibitors and SDS-PAGE (12.5%), and used in bioassays against the fungi. The electrophoretic profile revealed a single band in CfTI and eight bands in SPTI. The extracts of C. ferrea and S. polyphylla had an effect in reducing sporulation of the tested fungi, but S. rolfsii was inhibited only by C. ferrea. As to mycelial growth, the two extracts showed an effect on F. oxysporum and S. rolfsii, while C. guaranicola was inhibited only by S. polyphylla, and C. cassiicola by C. ferrea. It was concluded that seeds of C. ferrea and S. polyphylla present trypsin inhibitors. In addition, the results suggest that these tropical tree species are promising for the exploration of natural fungicides, since the extracts reduced mycelial growth and sporulation of C. guaranicola, C. cassiicola, F. oxysporum and S. rolfsii.Volume 38, Número 2, Pags. 131-138Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessAthelia RolfsiiCaesalpiniaCaesalpinia FerreaColletotrichumCorynespora CassiicolaFungiFusarium OxysporumSwartzia PolyphyllaPartial purification of trypsin inhibitors from Caesalpinia ferrea and Swartzia polyphylla seeds and effect of protein extracts on pathogenic fungiPurificação parcial de inibidores de tripsina de sementes de Caesalpinia ferrea e Swartzia polyphylla e o efeito dos extratos protéicos sobre fungos fitopatogênicosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSumma Phytopathologicaporreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa8.pdfartigo-inpa8.pdfapplication/pdf1274549https://repositorio.inpa.gov.br/bitstream/1/16135/1/artigo-inpa8.pdfe2d728a0223d1f2cd522af0d15a2fea7MD511/161352020-07-14 11:28:15.81oai:repositorio:1/16135Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T15:28:15Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.en.fl_str_mv Partial purification of trypsin inhibitors from Caesalpinia ferrea and Swartzia polyphylla seeds and effect of protein extracts on pathogenic fungi
dc.title.alternative.pt_BR.fl_str_mv Purificação parcial de inibidores de tripsina de sementes de Caesalpinia ferrea e Swartzia polyphylla e o efeito dos extratos protéicos sobre fungos fitopatogênicos
title Partial purification of trypsin inhibitors from Caesalpinia ferrea and Swartzia polyphylla seeds and effect of protein extracts on pathogenic fungi
spellingShingle Partial purification of trypsin inhibitors from Caesalpinia ferrea and Swartzia polyphylla seeds and effect of protein extracts on pathogenic fungi
Bariani, Adriana
Athelia Rolfsii
Caesalpinia
Caesalpinia Ferrea
Colletotrichum
Corynespora Cassiicola
Fungi
Fusarium Oxysporum
Swartzia Polyphylla
title_short Partial purification of trypsin inhibitors from Caesalpinia ferrea and Swartzia polyphylla seeds and effect of protein extracts on pathogenic fungi
title_full Partial purification of trypsin inhibitors from Caesalpinia ferrea and Swartzia polyphylla seeds and effect of protein extracts on pathogenic fungi
title_fullStr Partial purification of trypsin inhibitors from Caesalpinia ferrea and Swartzia polyphylla seeds and effect of protein extracts on pathogenic fungi
title_full_unstemmed Partial purification of trypsin inhibitors from Caesalpinia ferrea and Swartzia polyphylla seeds and effect of protein extracts on pathogenic fungi
title_sort Partial purification of trypsin inhibitors from Caesalpinia ferrea and Swartzia polyphylla seeds and effect of protein extracts on pathogenic fungi
author Bariani, Adriana
author_facet Bariani, Adriana
Gonçalves, José Francisco Carvalho de
Chevreuil, Larissa Ramos
Cavallazzi, José Renato Pereira
Souza, Luis Augusto Gomes de
Bentes, Jânia Lilia da Silva
Pando, Silvana Cristina
author_role author
author2 Gonçalves, José Francisco Carvalho de
Chevreuil, Larissa Ramos
Cavallazzi, José Renato Pereira
Souza, Luis Augusto Gomes de
Bentes, Jânia Lilia da Silva
Pando, Silvana Cristina
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bariani, Adriana
Gonçalves, José Francisco Carvalho de
Chevreuil, Larissa Ramos
Cavallazzi, José Renato Pereira
Souza, Luis Augusto Gomes de
Bentes, Jânia Lilia da Silva
Pando, Silvana Cristina
dc.subject.eng.fl_str_mv Athelia Rolfsii
Caesalpinia
Caesalpinia Ferrea
Colletotrichum
Corynespora Cassiicola
Fungi
Fusarium Oxysporum
Swartzia Polyphylla
topic Athelia Rolfsii
Caesalpinia
Caesalpinia Ferrea
Colletotrichum
Corynespora Cassiicola
Fungi
Fusarium Oxysporum
Swartzia Polyphylla
description Legume seeds present high concentration of trypsin inhibitors; these proteins are involved in both cell metabolism and plant defense mechanisms. In order to confirm or not a possible fungicidal action of these proteins from extracts of seeds of legume tree species, the aim this study was to detect trypsin inhibitors in seeds of Caesalpinia ferrea (CfTI) and Swartzia polyphylla (SpTI) and to test their extracts against the pathogenic fungi Colletotrichum guaranicola, Corynespora cassiicola, Fusarium oxysporum and Sclerotium rolfsii, evaluating mycelial growth and sporulation. Thus, samples of the plant material, finely powdered seeds, were extracted in 150 mM NaCl. The protein extracts were partially purified on Sephadex G-100 column, subjected to detection of inhibitors and SDS-PAGE (12.5%), and used in bioassays against the fungi. The electrophoretic profile revealed a single band in CfTI and eight bands in SPTI. The extracts of C. ferrea and S. polyphylla had an effect in reducing sporulation of the tested fungi, but S. rolfsii was inhibited only by C. ferrea. As to mycelial growth, the two extracts showed an effect on F. oxysporum and S. rolfsii, while C. guaranicola was inhibited only by S. polyphylla, and C. cassiicola by C. ferrea. It was concluded that seeds of C. ferrea and S. polyphylla present trypsin inhibitors. In addition, the results suggest that these tropical tree species are promising for the exploration of natural fungicides, since the extracts reduced mycelial growth and sporulation of C. guaranicola, C. cassiicola, F. oxysporum and S. rolfsii.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2020-05-24T22:15:13Z
dc.date.available.fl_str_mv 2020-05-24T22:15:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.inpa.gov.br/handle/1/16135
dc.identifier.doi.none.fl_str_mv 10.1590/S0100-54052012000200004
url https://repositorio.inpa.gov.br/handle/1/16135
identifier_str_mv 10.1590/S0100-54052012000200004
dc.language.iso.fl_str_mv por
language por
dc.relation.ispartof.pt_BR.fl_str_mv Volume 38, Número 2, Pags. 131-138
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Summa Phytopathologica
publisher.none.fl_str_mv Summa Phytopathologica
dc.source.none.fl_str_mv reponame:Repositório Institucional do INPA
instname:Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron:INPA
instname_str Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron_str INPA
institution INPA
reponame_str Repositório Institucional do INPA
collection Repositório Institucional do INPA
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