Partial purification of trypsin inhibitors from Caesalpinia ferrea and Swartzia polyphylla seeds and effect of protein extracts on pathogenic fungi
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/16135 |
Resumo: | Legume seeds present high concentration of trypsin inhibitors; these proteins are involved in both cell metabolism and plant defense mechanisms. In order to confirm or not a possible fungicidal action of these proteins from extracts of seeds of legume tree species, the aim this study was to detect trypsin inhibitors in seeds of Caesalpinia ferrea (CfTI) and Swartzia polyphylla (SpTI) and to test their extracts against the pathogenic fungi Colletotrichum guaranicola, Corynespora cassiicola, Fusarium oxysporum and Sclerotium rolfsii, evaluating mycelial growth and sporulation. Thus, samples of the plant material, finely powdered seeds, were extracted in 150 mM NaCl. The protein extracts were partially purified on Sephadex G-100 column, subjected to detection of inhibitors and SDS-PAGE (12.5%), and used in bioassays against the fungi. The electrophoretic profile revealed a single band in CfTI and eight bands in SPTI. The extracts of C. ferrea and S. polyphylla had an effect in reducing sporulation of the tested fungi, but S. rolfsii was inhibited only by C. ferrea. As to mycelial growth, the two extracts showed an effect on F. oxysporum and S. rolfsii, while C. guaranicola was inhibited only by S. polyphylla, and C. cassiicola by C. ferrea. It was concluded that seeds of C. ferrea and S. polyphylla present trypsin inhibitors. In addition, the results suggest that these tropical tree species are promising for the exploration of natural fungicides, since the extracts reduced mycelial growth and sporulation of C. guaranicola, C. cassiicola, F. oxysporum and S. rolfsii. |
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Bariani, AdrianaGonçalves, José Francisco Carvalho deChevreuil, Larissa RamosCavallazzi, José Renato PereiraSouza, Luis Augusto Gomes deBentes, Jânia Lilia da SilvaPando, Silvana Cristina2020-05-24T22:15:13Z2020-05-24T22:15:13Z2012https://repositorio.inpa.gov.br/handle/1/1613510.1590/S0100-54052012000200004Legume seeds present high concentration of trypsin inhibitors; these proteins are involved in both cell metabolism and plant defense mechanisms. In order to confirm or not a possible fungicidal action of these proteins from extracts of seeds of legume tree species, the aim this study was to detect trypsin inhibitors in seeds of Caesalpinia ferrea (CfTI) and Swartzia polyphylla (SpTI) and to test their extracts against the pathogenic fungi Colletotrichum guaranicola, Corynespora cassiicola, Fusarium oxysporum and Sclerotium rolfsii, evaluating mycelial growth and sporulation. Thus, samples of the plant material, finely powdered seeds, were extracted in 150 mM NaCl. The protein extracts were partially purified on Sephadex G-100 column, subjected to detection of inhibitors and SDS-PAGE (12.5%), and used in bioassays against the fungi. The electrophoretic profile revealed a single band in CfTI and eight bands in SPTI. The extracts of C. ferrea and S. polyphylla had an effect in reducing sporulation of the tested fungi, but S. rolfsii was inhibited only by C. ferrea. As to mycelial growth, the two extracts showed an effect on F. oxysporum and S. rolfsii, while C. guaranicola was inhibited only by S. polyphylla, and C. cassiicola by C. ferrea. It was concluded that seeds of C. ferrea and S. polyphylla present trypsin inhibitors. In addition, the results suggest that these tropical tree species are promising for the exploration of natural fungicides, since the extracts reduced mycelial growth and sporulation of C. guaranicola, C. cassiicola, F. oxysporum and S. rolfsii.Volume 38, Número 2, Pags. 131-138Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessAthelia RolfsiiCaesalpiniaCaesalpinia FerreaColletotrichumCorynespora CassiicolaFungiFusarium OxysporumSwartzia PolyphyllaPartial purification of trypsin inhibitors from Caesalpinia ferrea and Swartzia polyphylla seeds and effect of protein extracts on pathogenic fungiPurificação parcial de inibidores de tripsina de sementes de Caesalpinia ferrea e Swartzia polyphylla e o efeito dos extratos protéicos sobre fungos fitopatogênicosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSumma Phytopathologicaporreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa8.pdfartigo-inpa8.pdfapplication/pdf1274549https://repositorio.inpa.gov.br/bitstream/1/16135/1/artigo-inpa8.pdfe2d728a0223d1f2cd522af0d15a2fea7MD511/161352020-07-14 11:28:15.81oai:repositorio:1/16135Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T15:28:15Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Partial purification of trypsin inhibitors from Caesalpinia ferrea and Swartzia polyphylla seeds and effect of protein extracts on pathogenic fungi |
dc.title.alternative.pt_BR.fl_str_mv |
Purificação parcial de inibidores de tripsina de sementes de Caesalpinia ferrea e Swartzia polyphylla e o efeito dos extratos protéicos sobre fungos fitopatogênicos |
title |
Partial purification of trypsin inhibitors from Caesalpinia ferrea and Swartzia polyphylla seeds and effect of protein extracts on pathogenic fungi |
spellingShingle |
Partial purification of trypsin inhibitors from Caesalpinia ferrea and Swartzia polyphylla seeds and effect of protein extracts on pathogenic fungi Bariani, Adriana Athelia Rolfsii Caesalpinia Caesalpinia Ferrea Colletotrichum Corynespora Cassiicola Fungi Fusarium Oxysporum Swartzia Polyphylla |
title_short |
Partial purification of trypsin inhibitors from Caesalpinia ferrea and Swartzia polyphylla seeds and effect of protein extracts on pathogenic fungi |
title_full |
Partial purification of trypsin inhibitors from Caesalpinia ferrea and Swartzia polyphylla seeds and effect of protein extracts on pathogenic fungi |
title_fullStr |
Partial purification of trypsin inhibitors from Caesalpinia ferrea and Swartzia polyphylla seeds and effect of protein extracts on pathogenic fungi |
title_full_unstemmed |
Partial purification of trypsin inhibitors from Caesalpinia ferrea and Swartzia polyphylla seeds and effect of protein extracts on pathogenic fungi |
title_sort |
Partial purification of trypsin inhibitors from Caesalpinia ferrea and Swartzia polyphylla seeds and effect of protein extracts on pathogenic fungi |
author |
Bariani, Adriana |
author_facet |
Bariani, Adriana Gonçalves, José Francisco Carvalho de Chevreuil, Larissa Ramos Cavallazzi, José Renato Pereira Souza, Luis Augusto Gomes de Bentes, Jânia Lilia da Silva Pando, Silvana Cristina |
author_role |
author |
author2 |
Gonçalves, José Francisco Carvalho de Chevreuil, Larissa Ramos Cavallazzi, José Renato Pereira Souza, Luis Augusto Gomes de Bentes, Jânia Lilia da Silva Pando, Silvana Cristina |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Bariani, Adriana Gonçalves, José Francisco Carvalho de Chevreuil, Larissa Ramos Cavallazzi, José Renato Pereira Souza, Luis Augusto Gomes de Bentes, Jânia Lilia da Silva Pando, Silvana Cristina |
dc.subject.eng.fl_str_mv |
Athelia Rolfsii Caesalpinia Caesalpinia Ferrea Colletotrichum Corynespora Cassiicola Fungi Fusarium Oxysporum Swartzia Polyphylla |
topic |
Athelia Rolfsii Caesalpinia Caesalpinia Ferrea Colletotrichum Corynespora Cassiicola Fungi Fusarium Oxysporum Swartzia Polyphylla |
description |
Legume seeds present high concentration of trypsin inhibitors; these proteins are involved in both cell metabolism and plant defense mechanisms. In order to confirm or not a possible fungicidal action of these proteins from extracts of seeds of legume tree species, the aim this study was to detect trypsin inhibitors in seeds of Caesalpinia ferrea (CfTI) and Swartzia polyphylla (SpTI) and to test their extracts against the pathogenic fungi Colletotrichum guaranicola, Corynespora cassiicola, Fusarium oxysporum and Sclerotium rolfsii, evaluating mycelial growth and sporulation. Thus, samples of the plant material, finely powdered seeds, were extracted in 150 mM NaCl. The protein extracts were partially purified on Sephadex G-100 column, subjected to detection of inhibitors and SDS-PAGE (12.5%), and used in bioassays against the fungi. The electrophoretic profile revealed a single band in CfTI and eight bands in SPTI. The extracts of C. ferrea and S. polyphylla had an effect in reducing sporulation of the tested fungi, but S. rolfsii was inhibited only by C. ferrea. As to mycelial growth, the two extracts showed an effect on F. oxysporum and S. rolfsii, while C. guaranicola was inhibited only by S. polyphylla, and C. cassiicola by C. ferrea. It was concluded that seeds of C. ferrea and S. polyphylla present trypsin inhibitors. In addition, the results suggest that these tropical tree species are promising for the exploration of natural fungicides, since the extracts reduced mycelial growth and sporulation of C. guaranicola, C. cassiicola, F. oxysporum and S. rolfsii. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012 |
dc.date.accessioned.fl_str_mv |
2020-05-24T22:15:13Z |
dc.date.available.fl_str_mv |
2020-05-24T22:15:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/16135 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0100-54052012000200004 |
url |
https://repositorio.inpa.gov.br/handle/1/16135 |
identifier_str_mv |
10.1590/S0100-54052012000200004 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 38, Número 2, Pags. 131-138 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Summa Phytopathologica |
publisher.none.fl_str_mv |
Summa Phytopathologica |
dc.source.none.fl_str_mv |
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INPA |
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Repositório Institucional do INPA |
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