Cytogenetic data on six leafcutter ants of the genus Acromyrmex Mayr, 1865 (Hymenoptera, Formicidae, Myrmicinae): Insights into chromosome evolution and taxonomic implications
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/15864 |
Resumo: | Cytogenetic data for the genus Acromyrmex Mayr, 1865 are available, to date, for a few species from Brazil and Uruguay, which have uniform chromosome numbers (2n = 38). The recent cytogenetic data of Acromyrmex striatus (Roger, 1863), including its banding patterns, showed a distinct karyotype (2n = 22), similar to earlier studied Atta Fabricius, 1804 species. Karyological data are still scarce for the leafcutter ants and many gaps are still present for a proper understanding of this group. Therefore, this study aimed at increasing cytogenetic knowledge of the genus through the characterization of other six species: Acromyrmex balzani (Emery, 1890), A. coronatus Fabricius, 1804, A. disciger (Mayr, 1887), A. echinatior (Forel, 1899), A. niger (Smith, 1858) and A. rugosus (Smith, 1858), all of which were collected in Minas Gerais - Brazil, except for A. echinatior which was collected in Barro Colorado - Panama. The number and morphology of the chromosomes were studied and the following banding techniques were applied: C-banding, fluorochromes CMA3 and DAPI, as well as the detection of 45S rDNA using FISH technique. All the six species had the same chromosome number observed for already studied species, i.e. 2n = 38. A. balzani had a different karyotype compared with other species mainly due to the first metacentric pair. The heterochromatin distribution also showed interspecific variation. Nevertheless, all the studied species had a pair of bands in the short arm of the first subtelocentric pair. The fluorochrome CMA3 visualized bands in the short arm of the first subtelocentric pair for all the six species, while A. rugosus and A. niger also demonstrated in the other chromosomes. The AT-rich regions with differential staining using DAPI were not observed. 45S ribosomal genes were identified by FISH in the short arm of the first subtelocentric pair in A. coronatus, A. disciger and A. niger. The uniform chromosome number in the genus Acromyrmex (2n = 38) suggests that A. striatus (2n = 22) should be transferred to a new genus. Other aspects of the chromosome evolution in ants are also discussed. © Luísa A.C. Barros et al. |
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Barros, Luísa Antônia CamposAguiar, Hilton Jeferson Alves Cardoso deMariano, Cléa dos Santos FerreiraAndrade-Souza, V.Costa, Marco Antônio F.Delabie, Jacques H.C.Pompolo, Sílvia da Graças2020-05-19T19:58:43Z2020-05-19T19:58:43Z2016https://repositorio.inpa.gov.br/handle/1/1586410.3897/compcytogen.v10i2.7612Cytogenetic data for the genus Acromyrmex Mayr, 1865 are available, to date, for a few species from Brazil and Uruguay, which have uniform chromosome numbers (2n = 38). The recent cytogenetic data of Acromyrmex striatus (Roger, 1863), including its banding patterns, showed a distinct karyotype (2n = 22), similar to earlier studied Atta Fabricius, 1804 species. Karyological data are still scarce for the leafcutter ants and many gaps are still present for a proper understanding of this group. Therefore, this study aimed at increasing cytogenetic knowledge of the genus through the characterization of other six species: Acromyrmex balzani (Emery, 1890), A. coronatus Fabricius, 1804, A. disciger (Mayr, 1887), A. echinatior (Forel, 1899), A. niger (Smith, 1858) and A. rugosus (Smith, 1858), all of which were collected in Minas Gerais - Brazil, except for A. echinatior which was collected in Barro Colorado - Panama. The number and morphology of the chromosomes were studied and the following banding techniques were applied: C-banding, fluorochromes CMA3 and DAPI, as well as the detection of 45S rDNA using FISH technique. All the six species had the same chromosome number observed for already studied species, i.e. 2n = 38. A. balzani had a different karyotype compared with other species mainly due to the first metacentric pair. The heterochromatin distribution also showed interspecific variation. Nevertheless, all the studied species had a pair of bands in the short arm of the first subtelocentric pair. The fluorochrome CMA3 visualized bands in the short arm of the first subtelocentric pair for all the six species, while A. rugosus and A. niger also demonstrated in the other chromosomes. The AT-rich regions with differential staining using DAPI were not observed. 45S ribosomal genes were identified by FISH in the short arm of the first subtelocentric pair in A. coronatus, A. disciger and A. niger. The uniform chromosome number in the genus Acromyrmex (2n = 38) suggests that A. striatus (2n = 22) should be transferred to a new genus. Other aspects of the chromosome evolution in ants are also discussed. © Luísa A.C. Barros et al.Volume 10, Número 2, Pags. 229-243Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessCytogenetic data on six leafcutter ants of the genus Acromyrmex Mayr, 1865 (Hymenoptera, Formicidae, Myrmicinae): Insights into chromosome evolution and taxonomic implicationsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleComparative Cytogeneticsengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfartigo-inpa.pdfapplication/pdf1617124https://repositorio.inpa.gov.br/bitstream/1/15864/1/artigo-inpa.pdf9fcc13eca2184a8ef85861055d23a609MD511/158642020-05-19 16:06:37.344oai:repositorio:1/15864Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-05-19T20:06:37Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Cytogenetic data on six leafcutter ants of the genus Acromyrmex Mayr, 1865 (Hymenoptera, Formicidae, Myrmicinae): Insights into chromosome evolution and taxonomic implications |
title |
Cytogenetic data on six leafcutter ants of the genus Acromyrmex Mayr, 1865 (Hymenoptera, Formicidae, Myrmicinae): Insights into chromosome evolution and taxonomic implications |
spellingShingle |
Cytogenetic data on six leafcutter ants of the genus Acromyrmex Mayr, 1865 (Hymenoptera, Formicidae, Myrmicinae): Insights into chromosome evolution and taxonomic implications Barros, Luísa Antônia Campos |
title_short |
Cytogenetic data on six leafcutter ants of the genus Acromyrmex Mayr, 1865 (Hymenoptera, Formicidae, Myrmicinae): Insights into chromosome evolution and taxonomic implications |
title_full |
Cytogenetic data on six leafcutter ants of the genus Acromyrmex Mayr, 1865 (Hymenoptera, Formicidae, Myrmicinae): Insights into chromosome evolution and taxonomic implications |
title_fullStr |
Cytogenetic data on six leafcutter ants of the genus Acromyrmex Mayr, 1865 (Hymenoptera, Formicidae, Myrmicinae): Insights into chromosome evolution and taxonomic implications |
title_full_unstemmed |
Cytogenetic data on six leafcutter ants of the genus Acromyrmex Mayr, 1865 (Hymenoptera, Formicidae, Myrmicinae): Insights into chromosome evolution and taxonomic implications |
title_sort |
Cytogenetic data on six leafcutter ants of the genus Acromyrmex Mayr, 1865 (Hymenoptera, Formicidae, Myrmicinae): Insights into chromosome evolution and taxonomic implications |
author |
Barros, Luísa Antônia Campos |
author_facet |
Barros, Luísa Antônia Campos Aguiar, Hilton Jeferson Alves Cardoso de Mariano, Cléa dos Santos Ferreira Andrade-Souza, V. Costa, Marco Antônio F. Delabie, Jacques H.C. Pompolo, Sílvia da Graças |
author_role |
author |
author2 |
Aguiar, Hilton Jeferson Alves Cardoso de Mariano, Cléa dos Santos Ferreira Andrade-Souza, V. Costa, Marco Antônio F. Delabie, Jacques H.C. Pompolo, Sílvia da Graças |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Barros, Luísa Antônia Campos Aguiar, Hilton Jeferson Alves Cardoso de Mariano, Cléa dos Santos Ferreira Andrade-Souza, V. Costa, Marco Antônio F. Delabie, Jacques H.C. Pompolo, Sílvia da Graças |
description |
Cytogenetic data for the genus Acromyrmex Mayr, 1865 are available, to date, for a few species from Brazil and Uruguay, which have uniform chromosome numbers (2n = 38). The recent cytogenetic data of Acromyrmex striatus (Roger, 1863), including its banding patterns, showed a distinct karyotype (2n = 22), similar to earlier studied Atta Fabricius, 1804 species. Karyological data are still scarce for the leafcutter ants and many gaps are still present for a proper understanding of this group. Therefore, this study aimed at increasing cytogenetic knowledge of the genus through the characterization of other six species: Acromyrmex balzani (Emery, 1890), A. coronatus Fabricius, 1804, A. disciger (Mayr, 1887), A. echinatior (Forel, 1899), A. niger (Smith, 1858) and A. rugosus (Smith, 1858), all of which were collected in Minas Gerais - Brazil, except for A. echinatior which was collected in Barro Colorado - Panama. The number and morphology of the chromosomes were studied and the following banding techniques were applied: C-banding, fluorochromes CMA3 and DAPI, as well as the detection of 45S rDNA using FISH technique. All the six species had the same chromosome number observed for already studied species, i.e. 2n = 38. A. balzani had a different karyotype compared with other species mainly due to the first metacentric pair. The heterochromatin distribution also showed interspecific variation. Nevertheless, all the studied species had a pair of bands in the short arm of the first subtelocentric pair. The fluorochrome CMA3 visualized bands in the short arm of the first subtelocentric pair for all the six species, while A. rugosus and A. niger also demonstrated in the other chromosomes. The AT-rich regions with differential staining using DAPI were not observed. 45S ribosomal genes were identified by FISH in the short arm of the first subtelocentric pair in A. coronatus, A. disciger and A. niger. The uniform chromosome number in the genus Acromyrmex (2n = 38) suggests that A. striatus (2n = 22) should be transferred to a new genus. Other aspects of the chromosome evolution in ants are also discussed. © Luísa A.C. Barros et al. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2020-05-19T19:58:43Z |
dc.date.available.fl_str_mv |
2020-05-19T19:58:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/15864 |
dc.identifier.doi.none.fl_str_mv |
10.3897/compcytogen.v10i2.7612 |
url |
https://repositorio.inpa.gov.br/handle/1/15864 |
identifier_str_mv |
10.3897/compcytogen.v10i2.7612 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 10, Número 2, Pags. 229-243 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
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openAccess |
dc.publisher.none.fl_str_mv |
Comparative Cytogenetics |
publisher.none.fl_str_mv |
Comparative Cytogenetics |
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INPA |
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Repositório Institucional do INPA |
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Repositório Institucional do INPA |
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https://repositorio.inpa.gov.br/bitstream/1/15864/1/artigo-inpa.pdf |
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