The effect of graded methaemoglobin levels on the swimming performance in chinook salmon (Oncorhynchus tshawytscha)
Main Author: | |
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Publication Date: | 1992 |
Other Authors: | |
Format: | Conference object |
Language: | eng |
Source: | Repositório Institucional do INPA |
Download full: | https://repositorio.inpa.gov.br/handle/1/23332 |
Summary: | Nitrite oxidizes haemoglobin (Hb) to methaemoglobin (MetHb), which is unable to bind oxygen. Nitrite exposure can therefore be used as a tool to manipulate the oxygen-carrying capacity of the blood without changing haematocrit. The objective of this study is to examine the relationship between the critical swimming velocity (Ucrit) and the functional haemoglobin concentration ([Hb]) of the blood in adult chinook salmon. Functional [Hb] was reduced by increasing MetHb levels through intraperitoneal administration of a mass-dependent volume of sodium nitrite. In resting fish, MetHb levels were found to stabilize at 25 % of total [Hb] 3 h after the injection of 30 mg kg-1 sodium nitrite. Methaemoglobin levels increased in proportion to the amount of sodium nitrite injected and reached a maximum (following the injection of 90 mg kg-1 sodium nitrite) of 51.8 % in resting fish and 72 % in fish forced to swim to Ucrit. At 60 and 90 mg kg-1 sodium nitrite, MetHb formation was greater in exercised than in resting fish. A second-order regression revealed that Ucrit was virtually independent of functional [Hb] between 51 and 100 % of control functional [Hb], but was positively correlated with functional [Hb] below 51 % of total [Hb] (4.5 g dl-1). The insensitivity of Ucrit to a functional [Hb] greater than 51 % may be partly due to the exponential increase in aerobic metabolism required to provide the power to overcome hydrodynamic drag at higher water velocities. There were no significant changes in intraerythrocytic organic phosphate (adenylates and guanylates) concentrations standardized to [Hb] in swimming or resting fish over the range of MetHb levels induced in this study. Fish may encounter nitrite naturally; if MetHb levels become severely elevated as a result, swimming ability will be significantly impaired. |
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Val, Adalberto LuisBrauner, Colin Jonh2020-07-16T14:46:36Z2020-07-16T14:46:36Z1992https://repositorio.inpa.gov.br/handle/1/23332Journal of Experimental Biology 1993 185: 121-135Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessTrutaHemoglobinasNataçãoNitritoThe effect of graded methaemoglobin levels on the swimming performance in chinook salmon (Oncorhynchus tshawytscha)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectNitrite oxidizes haemoglobin (Hb) to methaemoglobin (MetHb), which is unable to bind oxygen. Nitrite exposure can therefore be used as a tool to manipulate the oxygen-carrying capacity of the blood without changing haematocrit. The objective of this study is to examine the relationship between the critical swimming velocity (Ucrit) and the functional haemoglobin concentration ([Hb]) of the blood in adult chinook salmon. Functional [Hb] was reduced by increasing MetHb levels through intraperitoneal administration of a mass-dependent volume of sodium nitrite. In resting fish, MetHb levels were found to stabilize at 25 % of total [Hb] 3 h after the injection of 30 mg kg-1 sodium nitrite. Methaemoglobin levels increased in proportion to the amount of sodium nitrite injected and reached a maximum (following the injection of 90 mg kg-1 sodium nitrite) of 51.8 % in resting fish and 72 % in fish forced to swim to Ucrit. At 60 and 90 mg kg-1 sodium nitrite, MetHb formation was greater in exercised than in resting fish. A second-order regression revealed that Ucrit was virtually independent of functional [Hb] between 51 and 100 % of control functional [Hb], but was positively correlated with functional [Hb] below 51 % of total [Hb] (4.5 g dl-1). The insensitivity of Ucrit to a functional [Hb] greater than 51 % may be partly due to the exponential increase in aerobic metabolism required to provide the power to overcome hydrodynamic drag at higher water velocities. There were no significant changes in intraerythrocytic organic phosphate (adenylates and guanylates) concentrations standardized to [Hb] in swimming or resting fish over the range of MetHb levels induced in this study. Fish may encounter nitrite naturally; if MetHb levels become severely elevated as a result, swimming ability will be significantly impaired.Annual Meeting of the Society for Experimental BiologyInglaterraengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALtrabalho-inpa.pdftrabalho-inpa.pdfapplication/pdf152638https://repositorio.inpa.gov.br/bitstream/1/23332/1/trabalho-inpa.pdfe771adc808a95e00d726410e9f6302aaMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.inpa.gov.br/bitstream/1/23332/2/license_rdfe39d27027a6cc9cb039ad269a5db8e34MD521/233322020-07-16 10:46:37.299oai:repositorio:1/23332Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-16T14:46:37Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.pt_BR.fl_str_mv |
The effect of graded methaemoglobin levels on the swimming performance in chinook salmon (Oncorhynchus tshawytscha) |
title |
The effect of graded methaemoglobin levels on the swimming performance in chinook salmon (Oncorhynchus tshawytscha) |
spellingShingle |
The effect of graded methaemoglobin levels on the swimming performance in chinook salmon (Oncorhynchus tshawytscha) Val, Adalberto Luis Truta Hemoglobinas Natação Nitrito |
title_short |
The effect of graded methaemoglobin levels on the swimming performance in chinook salmon (Oncorhynchus tshawytscha) |
title_full |
The effect of graded methaemoglobin levels on the swimming performance in chinook salmon (Oncorhynchus tshawytscha) |
title_fullStr |
The effect of graded methaemoglobin levels on the swimming performance in chinook salmon (Oncorhynchus tshawytscha) |
title_full_unstemmed |
The effect of graded methaemoglobin levels on the swimming performance in chinook salmon (Oncorhynchus tshawytscha) |
title_sort |
The effect of graded methaemoglobin levels on the swimming performance in chinook salmon (Oncorhynchus tshawytscha) |
author |
Val, Adalberto Luis |
author_facet |
Val, Adalberto Luis Brauner, Colin Jonh |
author_role |
author |
author2 |
Brauner, Colin Jonh |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Val, Adalberto Luis Brauner, Colin Jonh |
dc.subject.por.fl_str_mv |
Truta Hemoglobinas Natação Nitrito |
topic |
Truta Hemoglobinas Natação Nitrito |
description |
Nitrite oxidizes haemoglobin (Hb) to methaemoglobin (MetHb), which is unable to bind oxygen. Nitrite exposure can therefore be used as a tool to manipulate the oxygen-carrying capacity of the blood without changing haematocrit. The objective of this study is to examine the relationship between the critical swimming velocity (Ucrit) and the functional haemoglobin concentration ([Hb]) of the blood in adult chinook salmon. Functional [Hb] was reduced by increasing MetHb levels through intraperitoneal administration of a mass-dependent volume of sodium nitrite. In resting fish, MetHb levels were found to stabilize at 25 % of total [Hb] 3 h after the injection of 30 mg kg-1 sodium nitrite. Methaemoglobin levels increased in proportion to the amount of sodium nitrite injected and reached a maximum (following the injection of 90 mg kg-1 sodium nitrite) of 51.8 % in resting fish and 72 % in fish forced to swim to Ucrit. At 60 and 90 mg kg-1 sodium nitrite, MetHb formation was greater in exercised than in resting fish. A second-order regression revealed that Ucrit was virtually independent of functional [Hb] between 51 and 100 % of control functional [Hb], but was positively correlated with functional [Hb] below 51 % of total [Hb] (4.5 g dl-1). The insensitivity of Ucrit to a functional [Hb] greater than 51 % may be partly due to the exponential increase in aerobic metabolism required to provide the power to overcome hydrodynamic drag at higher water velocities. There were no significant changes in intraerythrocytic organic phosphate (adenylates and guanylates) concentrations standardized to [Hb] in swimming or resting fish over the range of MetHb levels induced in this study. Fish may encounter nitrite naturally; if MetHb levels become severely elevated as a result, swimming ability will be significantly impaired. |
publishDate |
1992 |
dc.date.issued.fl_str_mv |
1992 |
dc.date.accessioned.fl_str_mv |
2020-07-16T14:46:36Z |
dc.date.available.fl_str_mv |
2020-07-16T14:46:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/23332 |
url |
https://repositorio.inpa.gov.br/handle/1/23332 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Journal of Experimental Biology 1993 185: 121-135 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Annual Meeting of the Society for Experimental Biology |
dc.publisher.country.fl_str_mv |
Inglaterra |
publisher.none.fl_str_mv |
Annual Meeting of the Society for Experimental Biology |
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Repositório Institucional do INPA |
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