Evaluation of fluorescence in situ hybridisation (FISH) for the detection of fungi directly from blood cultures and cerebrospinal fluid from patients with suspected invasive mycoses

Detalhes bibliográficos
Autor(a) principal: Silva, Roberto Moreira da
Data de Publicação: 2015
Outros Autores: Silva Neto, João Ricardo da, Santos, Carla Silvana, Frickmann, Hagen, Poppert, Sven, Cruz, Kátia Santana, Koshikene, Daniela, Souza, João Vicente Braga de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional do INPA
Texto Completo: https://repositorio.inpa.gov.br/handle/1/15908
Resumo: The aim of this study was to evaluate the diagnostic performance of in-house FISH (fluorescence in situ hybridisation) procedures for the direct identification of invasive fungal infections in blood cultures and cerebrospinal fluid (CSF) samples and to compare these FISH results with those obtained using traditional microbiological techniques and PCR targeting of the ITS1 region of the rRNA gene. In total, 112 CSF samples and 30 positive blood cultures were investigated by microscopic examination, culture, PCR-RFLP and FISH. The sensitivity of FISH for fungal infections in CSF proved to be slightly better than that of conventional microscopy (India ink) under the experimental conditions, detecting 48 (instead of 46) infections in 112 samples. The discriminatory powers of traditional microbiology, PCR-RFLP and FISH for fungal bloodstream infections were equivalent, with the detection of 14 fungal infections in 30 samples. However, the mean times to diagnosis after the detection of microbial growth by automated blood culture systems were 5 hours, 20 hours and 6 days for FISH, PCR-RFLP and traditional microbiology, respectively. The results demonstrate that FISH is a valuable tool for the identification of invasive mycoses that can be implemented in the diagnostic routine of hospital laboratories. © 2015 Da Silva et al.
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spelling Silva, Roberto Moreira daSilva Neto, João Ricardo daSantos, Carla SilvanaFrickmann, HagenPoppert, SvenCruz, Kátia SantanaKoshikene, DanielaSouza, João Vicente Braga de2020-05-20T14:15:52Z2020-05-20T14:15:52Z2015https://repositorio.inpa.gov.br/handle/1/1590810.1186/s12941-015-0065-5The aim of this study was to evaluate the diagnostic performance of in-house FISH (fluorescence in situ hybridisation) procedures for the direct identification of invasive fungal infections in blood cultures and cerebrospinal fluid (CSF) samples and to compare these FISH results with those obtained using traditional microbiological techniques and PCR targeting of the ITS1 region of the rRNA gene. In total, 112 CSF samples and 30 positive blood cultures were investigated by microscopic examination, culture, PCR-RFLP and FISH. The sensitivity of FISH for fungal infections in CSF proved to be slightly better than that of conventional microscopy (India ink) under the experimental conditions, detecting 48 (instead of 46) infections in 112 samples. The discriminatory powers of traditional microbiology, PCR-RFLP and FISH for fungal bloodstream infections were equivalent, with the detection of 14 fungal infections in 30 samples. However, the mean times to diagnosis after the detection of microbial growth by automated blood culture systems were 5 hours, 20 hours and 6 days for FISH, PCR-RFLP and traditional microbiology, respectively. The results demonstrate that FISH is a valuable tool for the identification of invasive mycoses that can be implemented in the diagnostic routine of hospital laboratories. © 2015 Da Silva et al.Volume 14, Número 1Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessAdolescentAdultBlood CultureBloodstream InfectionCerebrospinal FluidChildControlled StudyCryptococcosisDiagnostic Test Accuracy StudyFemaleIn Situ Hybridization, FluorescenceFungal DetectionHospital LaboratoryHumanMajor Clinical StudyMaleMicrobial GrowthPolymorphism, Restriction Fragment LengthSensitivity And SpecificitySystemic MycosisBloodClassificationEvaluation StudyIn Situ Hybridization, FluorescenceFungusGeneticsIsolation And PurificationMicrobiologyMycosesPolymerase Chain ReactionProceduresDna, FungalAdultBloodDna, FungalFemaleFungiHumansIn Situ Hybridization, FluorescenceMaleMycosesPolymerase Chain ReactionEvaluation of fluorescence in situ hybridisation (FISH) for the detection of fungi directly from blood cultures and cerebrospinal fluid from patients with suspected invasive mycosesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAnnals of Clinical Microbiology and Antimicrobialsengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfartigo-inpa.pdfapplication/pdf606323https://repositorio.inpa.gov.br/bitstream/1/15908/1/artigo-inpa.pdfc29f43a4f83494e4c24c5120b00d60fcMD511/159082020-05-20 11:07:45.972oai:repositorio:1/15908Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-05-20T15:07:45Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.en.fl_str_mv Evaluation of fluorescence in situ hybridisation (FISH) for the detection of fungi directly from blood cultures and cerebrospinal fluid from patients with suspected invasive mycoses
title Evaluation of fluorescence in situ hybridisation (FISH) for the detection of fungi directly from blood cultures and cerebrospinal fluid from patients with suspected invasive mycoses
spellingShingle Evaluation of fluorescence in situ hybridisation (FISH) for the detection of fungi directly from blood cultures and cerebrospinal fluid from patients with suspected invasive mycoses
Silva, Roberto Moreira da
Adolescent
Adult
Blood Culture
Bloodstream Infection
Cerebrospinal Fluid
Child
Controlled Study
Cryptococcosis
Diagnostic Test Accuracy Study
Female
In Situ Hybridization, Fluorescence
Fungal Detection
Hospital Laboratory
Human
Major Clinical Study
Male
Microbial Growth
Polymorphism, Restriction Fragment Length
Sensitivity And Specificity
Systemic Mycosis
Blood
Classification
Evaluation Study
In Situ Hybridization, Fluorescence
Fungus
Genetics
Isolation And Purification
Microbiology
Mycoses
Polymerase Chain Reaction
Procedures
Dna, Fungal
Adult
Blood
Dna, Fungal
Female
Fungi
Humans
In Situ Hybridization, Fluorescence
Male
Mycoses
Polymerase Chain Reaction
title_short Evaluation of fluorescence in situ hybridisation (FISH) for the detection of fungi directly from blood cultures and cerebrospinal fluid from patients with suspected invasive mycoses
title_full Evaluation of fluorescence in situ hybridisation (FISH) for the detection of fungi directly from blood cultures and cerebrospinal fluid from patients with suspected invasive mycoses
title_fullStr Evaluation of fluorescence in situ hybridisation (FISH) for the detection of fungi directly from blood cultures and cerebrospinal fluid from patients with suspected invasive mycoses
title_full_unstemmed Evaluation of fluorescence in situ hybridisation (FISH) for the detection of fungi directly from blood cultures and cerebrospinal fluid from patients with suspected invasive mycoses
title_sort Evaluation of fluorescence in situ hybridisation (FISH) for the detection of fungi directly from blood cultures and cerebrospinal fluid from patients with suspected invasive mycoses
author Silva, Roberto Moreira da
author_facet Silva, Roberto Moreira da
Silva Neto, João Ricardo da
Santos, Carla Silvana
Frickmann, Hagen
Poppert, Sven
Cruz, Kátia Santana
Koshikene, Daniela
Souza, João Vicente Braga de
author_role author
author2 Silva Neto, João Ricardo da
Santos, Carla Silvana
Frickmann, Hagen
Poppert, Sven
Cruz, Kátia Santana
Koshikene, Daniela
Souza, João Vicente Braga de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Roberto Moreira da
Silva Neto, João Ricardo da
Santos, Carla Silvana
Frickmann, Hagen
Poppert, Sven
Cruz, Kátia Santana
Koshikene, Daniela
Souza, João Vicente Braga de
dc.subject.eng.fl_str_mv Adolescent
Adult
Blood Culture
Bloodstream Infection
Cerebrospinal Fluid
Child
Controlled Study
Cryptococcosis
Diagnostic Test Accuracy Study
Female
In Situ Hybridization, Fluorescence
Fungal Detection
Hospital Laboratory
Human
Major Clinical Study
Male
Microbial Growth
Polymorphism, Restriction Fragment Length
Sensitivity And Specificity
Systemic Mycosis
Blood
Classification
Evaluation Study
In Situ Hybridization, Fluorescence
Fungus
Genetics
Isolation And Purification
Microbiology
Mycoses
Polymerase Chain Reaction
Procedures
Dna, Fungal
Adult
Blood
Dna, Fungal
Female
Fungi
Humans
In Situ Hybridization, Fluorescence
Male
Mycoses
Polymerase Chain Reaction
topic Adolescent
Adult
Blood Culture
Bloodstream Infection
Cerebrospinal Fluid
Child
Controlled Study
Cryptococcosis
Diagnostic Test Accuracy Study
Female
In Situ Hybridization, Fluorescence
Fungal Detection
Hospital Laboratory
Human
Major Clinical Study
Male
Microbial Growth
Polymorphism, Restriction Fragment Length
Sensitivity And Specificity
Systemic Mycosis
Blood
Classification
Evaluation Study
In Situ Hybridization, Fluorescence
Fungus
Genetics
Isolation And Purification
Microbiology
Mycoses
Polymerase Chain Reaction
Procedures
Dna, Fungal
Adult
Blood
Dna, Fungal
Female
Fungi
Humans
In Situ Hybridization, Fluorescence
Male
Mycoses
Polymerase Chain Reaction
description The aim of this study was to evaluate the diagnostic performance of in-house FISH (fluorescence in situ hybridisation) procedures for the direct identification of invasive fungal infections in blood cultures and cerebrospinal fluid (CSF) samples and to compare these FISH results with those obtained using traditional microbiological techniques and PCR targeting of the ITS1 region of the rRNA gene. In total, 112 CSF samples and 30 positive blood cultures were investigated by microscopic examination, culture, PCR-RFLP and FISH. The sensitivity of FISH for fungal infections in CSF proved to be slightly better than that of conventional microscopy (India ink) under the experimental conditions, detecting 48 (instead of 46) infections in 112 samples. The discriminatory powers of traditional microbiology, PCR-RFLP and FISH for fungal bloodstream infections were equivalent, with the detection of 14 fungal infections in 30 samples. However, the mean times to diagnosis after the detection of microbial growth by automated blood culture systems were 5 hours, 20 hours and 6 days for FISH, PCR-RFLP and traditional microbiology, respectively. The results demonstrate that FISH is a valuable tool for the identification of invasive mycoses that can be implemented in the diagnostic routine of hospital laboratories. © 2015 Da Silva et al.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2020-05-20T14:15:52Z
dc.date.available.fl_str_mv 2020-05-20T14:15:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.inpa.gov.br/handle/1/15908
dc.identifier.doi.none.fl_str_mv 10.1186/s12941-015-0065-5
url https://repositorio.inpa.gov.br/handle/1/15908
identifier_str_mv 10.1186/s12941-015-0065-5
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Volume 14, Número 1
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Annals of Clinical Microbiology and Antimicrobials
publisher.none.fl_str_mv Annals of Clinical Microbiology and Antimicrobials
dc.source.none.fl_str_mv reponame:Repositório Institucional do INPA
instname:Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron:INPA
instname_str Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron_str INPA
institution INPA
reponame_str Repositório Institucional do INPA
collection Repositório Institucional do INPA
bitstream.url.fl_str_mv https://repositorio.inpa.gov.br/bitstream/1/15908/1/artigo-inpa.pdf
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