One thousand DNA barcodes of piranhas and pacus reveal geographic structure and unrecognised diversity in the Amazon
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/15234 |
Resumo: | Piranhas and pacus (Characiformes: Serrasalmidae) are a charismatic but understudied family of Neotropical fishes. Here, we analyse a DNA barcode dataset comprising 1,122 specimens, 69 species, 16 genera, 208 localities, and 34 major river drainages in order to make an inventory of diversity and to highlight taxa and biogeographic areas worthy of further sampling effort and conservation protection. Using four methods of species discovery - incorporating both tree and distance based techniques - we report between 76 and 99 species-like clusters, i.e. between 20% and 33% of a priori identified taxonomic species were represented by more than one mtDNA lineage. There was a high degree of congruence between clusters, with 60% supported by three or four methods. Pacus of the genus Myloplus exhibited the most intraspecific variation, with six of the 13 species sampled found to have multiple lineages. Conversely, piranhas of the Serrasalmus rhombeus group proved difficult to delimit with these methods due to genetic similarity and polyphyly. Overall, our results recognise substantially underestimated diversity in the serrasalmids, and emphasise the Guiana and Brazilian Shield rivers as biogeographically important areas with multiple cases of across-shield and within-shield diversifications. We additionally highlight the distinctiveness and complex phylogeographic history of rheophilic taxa in particular, and suggest multiple colonisations of these habitats by different serrasalmid lineages. © 2018 The Author(s). |
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Machado, Valéria NogueiraCollins, Rupert A.Ota, Rafaela PriscilaAndrade, Marcelo C.Farias, Izeni P.Hrbek, Tomas2020-05-07T14:14:55Z2020-05-07T14:14:55Z2018https://repositorio.inpa.gov.br/handle/1/1523410.1038/s41598-018-26550-xPiranhas and pacus (Characiformes: Serrasalmidae) are a charismatic but understudied family of Neotropical fishes. Here, we analyse a DNA barcode dataset comprising 1,122 specimens, 69 species, 16 genera, 208 localities, and 34 major river drainages in order to make an inventory of diversity and to highlight taxa and biogeographic areas worthy of further sampling effort and conservation protection. Using four methods of species discovery - incorporating both tree and distance based techniques - we report between 76 and 99 species-like clusters, i.e. between 20% and 33% of a priori identified taxonomic species were represented by more than one mtDNA lineage. There was a high degree of congruence between clusters, with 60% supported by three or four methods. Pacus of the genus Myloplus exhibited the most intraspecific variation, with six of the 13 species sampled found to have multiple lineages. Conversely, piranhas of the Serrasalmus rhombeus group proved difficult to delimit with these methods due to genetic similarity and polyphyly. Overall, our results recognise substantially underestimated diversity in the serrasalmids, and emphasise the Guiana and Brazilian Shield rivers as biogeographically important areas with multiple cases of across-shield and within-shield diversifications. We additionally highlight the distinctiveness and complex phylogeographic history of rheophilic taxa in particular, and suggest multiple colonisations of these habitats by different serrasalmid lineages. © 2018 The Author(s).Volume 8, Número 1Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessAnimalsBiodiversityCharaciformesClassificationDna BarcodingGene LocusGeneticsGeographyPhylogenyAnimalBiodiversityCharaciformesDna Barcoding, TaxonomicGenetic LociGeographyPhylogenyOne thousand DNA barcodes of piranhas and pacus reveal geographic structure and unrecognised diversity in the Amazoninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleScientific Reportsengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfapplication/pdf5669386https://repositorio.inpa.gov.br/bitstream/1/15234/1/artigo-inpa.pdf7545c193f2b3665680ab14f2d471b2fdMD51CC-LICENSElicense_rdfapplication/octet-stream914https://repositorio.inpa.gov.br/bitstream/1/15234/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD521/152342020-07-14 11:00:22.343oai:repositorio:1/15234Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T15:00:22Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
One thousand DNA barcodes of piranhas and pacus reveal geographic structure and unrecognised diversity in the Amazon |
title |
One thousand DNA barcodes of piranhas and pacus reveal geographic structure and unrecognised diversity in the Amazon |
spellingShingle |
One thousand DNA barcodes of piranhas and pacus reveal geographic structure and unrecognised diversity in the Amazon Machado, Valéria Nogueira Animals Biodiversity Characiformes Classification Dna Barcoding Gene Locus Genetics Geography Phylogeny Animal Biodiversity Characiformes Dna Barcoding, Taxonomic Genetic Loci Geography Phylogeny |
title_short |
One thousand DNA barcodes of piranhas and pacus reveal geographic structure and unrecognised diversity in the Amazon |
title_full |
One thousand DNA barcodes of piranhas and pacus reveal geographic structure and unrecognised diversity in the Amazon |
title_fullStr |
One thousand DNA barcodes of piranhas and pacus reveal geographic structure and unrecognised diversity in the Amazon |
title_full_unstemmed |
One thousand DNA barcodes of piranhas and pacus reveal geographic structure and unrecognised diversity in the Amazon |
title_sort |
One thousand DNA barcodes of piranhas and pacus reveal geographic structure and unrecognised diversity in the Amazon |
author |
Machado, Valéria Nogueira |
author_facet |
Machado, Valéria Nogueira Collins, Rupert A. Ota, Rafaela Priscila Andrade, Marcelo C. Farias, Izeni P. Hrbek, Tomas |
author_role |
author |
author2 |
Collins, Rupert A. Ota, Rafaela Priscila Andrade, Marcelo C. Farias, Izeni P. Hrbek, Tomas |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Machado, Valéria Nogueira Collins, Rupert A. Ota, Rafaela Priscila Andrade, Marcelo C. Farias, Izeni P. Hrbek, Tomas |
dc.subject.eng.fl_str_mv |
Animals Biodiversity Characiformes Classification Dna Barcoding Gene Locus Genetics Geography Phylogeny Animal Biodiversity Characiformes Dna Barcoding, Taxonomic Genetic Loci Geography Phylogeny |
topic |
Animals Biodiversity Characiformes Classification Dna Barcoding Gene Locus Genetics Geography Phylogeny Animal Biodiversity Characiformes Dna Barcoding, Taxonomic Genetic Loci Geography Phylogeny |
description |
Piranhas and pacus (Characiformes: Serrasalmidae) are a charismatic but understudied family of Neotropical fishes. Here, we analyse a DNA barcode dataset comprising 1,122 specimens, 69 species, 16 genera, 208 localities, and 34 major river drainages in order to make an inventory of diversity and to highlight taxa and biogeographic areas worthy of further sampling effort and conservation protection. Using four methods of species discovery - incorporating both tree and distance based techniques - we report between 76 and 99 species-like clusters, i.e. between 20% and 33% of a priori identified taxonomic species were represented by more than one mtDNA lineage. There was a high degree of congruence between clusters, with 60% supported by three or four methods. Pacus of the genus Myloplus exhibited the most intraspecific variation, with six of the 13 species sampled found to have multiple lineages. Conversely, piranhas of the Serrasalmus rhombeus group proved difficult to delimit with these methods due to genetic similarity and polyphyly. Overall, our results recognise substantially underestimated diversity in the serrasalmids, and emphasise the Guiana and Brazilian Shield rivers as biogeographically important areas with multiple cases of across-shield and within-shield diversifications. We additionally highlight the distinctiveness and complex phylogeographic history of rheophilic taxa in particular, and suggest multiple colonisations of these habitats by different serrasalmid lineages. © 2018 The Author(s). |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2020-05-07T14:14:55Z |
dc.date.available.fl_str_mv |
2020-05-07T14:14:55Z |
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article |
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dc.identifier.doi.none.fl_str_mv |
10.1038/s41598-018-26550-x |
url |
https://repositorio.inpa.gov.br/handle/1/15234 |
identifier_str_mv |
10.1038/s41598-018-26550-x |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Volume 8, Número 1 |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
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openAccess |
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Scientific Reports |
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Scientific Reports |
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