Mapping of ribosomal genes and chromosomal markers in three species of the genus Serrasalmus (Characidae, Serrasalminae) from the Amazon basin
Main Author: | |
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Publication Date: | 2008 |
Other Authors: | , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional do INPA |
Download full: | https://repositorio.inpa.gov.br/handle/1/14960 |
Summary: | Karyotypic characteristics of three species of the genus serrasalmus (S. altispinnis, S. gouldingi and S. Serrulatus) from the middle and lower Negro River, Amazon Basin, were investigated using different staining techniques and Fluorescent in situ hybridization with 5S and 18S rDNA probes. The diploid number was invariably 2n = 60 and the fundamental number was FN = 110. Nevertheless, the karyotypes differed from each other in composition: 24m, 20sm, 6st, 10a in S. altispinnis; 22m, 22sm, 6st, 10a in S. gouldingi and 20m, 22sm, 8st, 10a in S. serrulatus. The karyotype of S. altispinnis differed from the one previously described in a population from the Pitinga River. C-positive constitutive heterochromatin was mainly pericentromeric in the karyotypes of all species. Nucleolar organizer regions were multiple and preferentially located terminally on the short arms of the subtelocentric/acrocentric chromosomes, as evidenced by both silver nitrate staining and fluorescent in situ hybridization with the 18S rDNA probe. The maximum number of NORs varied among species, as did the NOR-bearing chromosomes. FISH with the 5S rDNA probe produced an interstitial signal on the long arms of the pair 7 in all species, coincident with a C-positive heterochromatic band. While some chromosome features were shared by the three species, some were species-specific and thus useful for cytotaxonomy. Copyright © 2008, Sociedade Brasileira de Genética. |
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Nakayama, Celeste MutukoFeldberg, ElianaBertollo, Luiz Antônio Carlos2020-05-07T13:59:09Z2020-05-07T13:59:09Z2008https://repositorio.inpa.gov.br/handle/1/1496010.1590/S1415-47572008005000018Karyotypic characteristics of three species of the genus serrasalmus (S. altispinnis, S. gouldingi and S. Serrulatus) from the middle and lower Negro River, Amazon Basin, were investigated using different staining techniques and Fluorescent in situ hybridization with 5S and 18S rDNA probes. The diploid number was invariably 2n = 60 and the fundamental number was FN = 110. Nevertheless, the karyotypes differed from each other in composition: 24m, 20sm, 6st, 10a in S. altispinnis; 22m, 22sm, 6st, 10a in S. gouldingi and 20m, 22sm, 8st, 10a in S. serrulatus. The karyotype of S. altispinnis differed from the one previously described in a population from the Pitinga River. C-positive constitutive heterochromatin was mainly pericentromeric in the karyotypes of all species. Nucleolar organizer regions were multiple and preferentially located terminally on the short arms of the subtelocentric/acrocentric chromosomes, as evidenced by both silver nitrate staining and fluorescent in situ hybridization with the 18S rDNA probe. The maximum number of NORs varied among species, as did the NOR-bearing chromosomes. FISH with the 5S rDNA probe produced an interstitial signal on the long arms of the pair 7 in all species, coincident with a C-positive heterochromatic band. While some chromosome features were shared by the three species, some were species-specific and thus useful for cytotaxonomy. Copyright © 2008, Sociedade Brasileira de Genética.Volume 31, Número 4, Pags. 868-873Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessDna 18sDna 5sSilver NitrateAcrocentric ChromosomeAnimals TissueBrasilChromosome MarkerChromosome NorControlled StudyCytotaxonomyDiploidyDna ProbeFish GeneticsIn Situ Hybridization, FluorescenceGene MappingHeterochromatinKaryotypingNonhumanRiver BasinSignal TransductionSpecies DifferenceStainingCharacidaeCharaciformesSerrasalminaeSerrasalmusSerrasalmus GouldingiMapping of ribosomal genes and chromosomal markers in three species of the genus Serrasalmus (Characidae, Serrasalminae) from the Amazon basininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Biologyengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfapplication/pdf312957https://repositorio.inpa.gov.br/bitstream/1/14960/1/artigo-inpa.pdfdba58ed24b1f6aeec5de084315e59c8cMD51CC-LICENSElicense_rdfapplication/octet-stream914https://repositorio.inpa.gov.br/bitstream/1/14960/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD521/149602020-07-14 10:29:57.589oai:repositorio:1/14960Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T14:29:57Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Mapping of ribosomal genes and chromosomal markers in three species of the genus Serrasalmus (Characidae, Serrasalminae) from the Amazon basin |
title |
Mapping of ribosomal genes and chromosomal markers in three species of the genus Serrasalmus (Characidae, Serrasalminae) from the Amazon basin |
spellingShingle |
Mapping of ribosomal genes and chromosomal markers in three species of the genus Serrasalmus (Characidae, Serrasalminae) from the Amazon basin Nakayama, Celeste Mutuko Dna 18s Dna 5s Silver Nitrate Acrocentric Chromosome Animals Tissue Brasil Chromosome Marker Chromosome Nor Controlled Study Cytotaxonomy Diploidy Dna Probe Fish Genetics In Situ Hybridization, Fluorescence Gene Mapping Heterochromatin Karyotyping Nonhuman River Basin Signal Transduction Species Difference Staining Characidae Characiformes Serrasalminae Serrasalmus Serrasalmus Gouldingi |
title_short |
Mapping of ribosomal genes and chromosomal markers in three species of the genus Serrasalmus (Characidae, Serrasalminae) from the Amazon basin |
title_full |
Mapping of ribosomal genes and chromosomal markers in three species of the genus Serrasalmus (Characidae, Serrasalminae) from the Amazon basin |
title_fullStr |
Mapping of ribosomal genes and chromosomal markers in three species of the genus Serrasalmus (Characidae, Serrasalminae) from the Amazon basin |
title_full_unstemmed |
Mapping of ribosomal genes and chromosomal markers in three species of the genus Serrasalmus (Characidae, Serrasalminae) from the Amazon basin |
title_sort |
Mapping of ribosomal genes and chromosomal markers in three species of the genus Serrasalmus (Characidae, Serrasalminae) from the Amazon basin |
author |
Nakayama, Celeste Mutuko |
author_facet |
Nakayama, Celeste Mutuko Feldberg, Eliana Bertollo, Luiz Antônio Carlos |
author_role |
author |
author2 |
Feldberg, Eliana Bertollo, Luiz Antônio Carlos |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Nakayama, Celeste Mutuko Feldberg, Eliana Bertollo, Luiz Antônio Carlos |
dc.subject.eng.fl_str_mv |
Dna 18s Dna 5s Silver Nitrate Acrocentric Chromosome Animals Tissue Brasil Chromosome Marker Chromosome Nor Controlled Study Cytotaxonomy Diploidy Dna Probe Fish Genetics In Situ Hybridization, Fluorescence Gene Mapping Heterochromatin Karyotyping Nonhuman River Basin Signal Transduction Species Difference Staining Characidae Characiformes Serrasalminae Serrasalmus Serrasalmus Gouldingi |
topic |
Dna 18s Dna 5s Silver Nitrate Acrocentric Chromosome Animals Tissue Brasil Chromosome Marker Chromosome Nor Controlled Study Cytotaxonomy Diploidy Dna Probe Fish Genetics In Situ Hybridization, Fluorescence Gene Mapping Heterochromatin Karyotyping Nonhuman River Basin Signal Transduction Species Difference Staining Characidae Characiformes Serrasalminae Serrasalmus Serrasalmus Gouldingi |
description |
Karyotypic characteristics of three species of the genus serrasalmus (S. altispinnis, S. gouldingi and S. Serrulatus) from the middle and lower Negro River, Amazon Basin, were investigated using different staining techniques and Fluorescent in situ hybridization with 5S and 18S rDNA probes. The diploid number was invariably 2n = 60 and the fundamental number was FN = 110. Nevertheless, the karyotypes differed from each other in composition: 24m, 20sm, 6st, 10a in S. altispinnis; 22m, 22sm, 6st, 10a in S. gouldingi and 20m, 22sm, 8st, 10a in S. serrulatus. The karyotype of S. altispinnis differed from the one previously described in a population from the Pitinga River. C-positive constitutive heterochromatin was mainly pericentromeric in the karyotypes of all species. Nucleolar organizer regions were multiple and preferentially located terminally on the short arms of the subtelocentric/acrocentric chromosomes, as evidenced by both silver nitrate staining and fluorescent in situ hybridization with the 18S rDNA probe. The maximum number of NORs varied among species, as did the NOR-bearing chromosomes. FISH with the 5S rDNA probe produced an interstitial signal on the long arms of the pair 7 in all species, coincident with a C-positive heterochromatic band. While some chromosome features were shared by the three species, some were species-specific and thus useful for cytotaxonomy. Copyright © 2008, Sociedade Brasileira de Genética. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008 |
dc.date.accessioned.fl_str_mv |
2020-05-07T13:59:09Z |
dc.date.available.fl_str_mv |
2020-05-07T13:59:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/14960 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S1415-47572008005000018 |
url |
https://repositorio.inpa.gov.br/handle/1/14960 |
identifier_str_mv |
10.1590/S1415-47572008005000018 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 31, Número 4, Pags. 868-873 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Genetics and Molecular Biology |
publisher.none.fl_str_mv |
Genetics and Molecular Biology |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional do INPA instname:Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
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INPA |
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Repositório Institucional do INPA |
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