Roundup® exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macropomum

• Autor(a) principal: Braz-Mota, Susana
• Data de Publicação: 2015
• Outros Autores: Sadauskas-Henrique, Helen, Duarte, Rafael Mendonça, Val, Adalberto Luis, Almeida-Val, Vera Maria Fonseca
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional do INPA
• Texto Completo: https://repositorio.inpa.gov.br/handle/1/15911
• topic: Aquaculture; Biomarkers; Brain; Herbicides; Weed Control; Anthropogenic Pressures; Biotransformation Process; Cholinesterase Activity; Glyphosates; Hematological Parameters; Reactive Oxygen Species; Respiratory Epitheliums; Tambaqui; Fish; Antioxidant; Biological Marker; Dna; Glucose; Glyphosate; Hemoglobin; Reactive Oxygen Metabolite; Roundup; Unclassified Drug; Cholinergic Receptor Stimulating Agent; Glycine; Glyphosate; Herbicide; Water Pollutant; Bioaccumulation; Biomarker; Biotransformation; Brain; Dna; Herbicide; Pollution Effect; Pollution Exposure; River Pollution; Teleost; Animals Experiment; Animals Tissue; Biotransformation; Cholinergic Activity; Colossoma Macropomum; Concentration (parameters); Controlled Study; Dna Damage; Environmental Exposure; Erythrocyte; Gill; Glucose Blood Level; Hemoglobin Determination; Histopathology; Juvenile Animals; Lc50; Liver; Neurologic Disease; Nonhuman; Respiratory Epithelium; Teleost; Toxicity Testing; Analogs And Derivatives; Animals; Brain; Dna Damage; Drug Effects; Fish; Gill; Liver; Metabolism; Physiology; Toxicity; Water Pollutant; Amazon River; Animalsia; Colossoma Macropomum; Colossoma Marcopomum; Teleostei; Animal; Brain; Cholinergic Agents; Dna Damage; Fishes; Gills; Glycine; Herbicides; Liver; Water Pollutants, Chemical

Roundup Original® (RD) is a glyphosate-based herbicide used to control weeds in agriculture. Contamination of Amazon waters has increased as a consequence of anthropogenic pressure, including the use of herbicides as RD. The central goal of this study was to evaluate the toxic effects of RD on juveniles of tambaqui (Colossoma macropomum). Our findings show that biomarkers in tambaqui are organ specific and dependent on RD concentration. Alterations in gills structural and respiratory epithelium were followed by changes in hematological parameters such as concentration of hemoglobin, particularly in fish exposed to the higher concentration tested (75% of RD LC<inf>50</inf> 96h). In addition, both RD concentrations affected the biotransformation process in gills of tambaqui negatively. Instead, liver responses suggest that a production of reactive oxygen species (ROS) occurred in fish exposed to RD, particularly in the animals exposed to 75% RD, as seen by imbalances in biotransformation and antioxidant systems. The increased DNA damage observed in red blood cells of tambaqui exposed to RD is in agreement with this hypothesis. Finally, both tested sub-lethal concentrations of RD markedly inhibited the cholinesterase activity in fish brain. Thus, we can suggest that RD is potentially toxic to tambaqui and possibly to other tropical fish species. © 2015 Elsevier Ltd.