Roundup® exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macropomum
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/15911 |
Resumo: | Roundup Original® (RD) is a glyphosate-based herbicide used to control weeds in agriculture. Contamination of Amazon waters has increased as a consequence of anthropogenic pressure, including the use of herbicides as RD. The central goal of this study was to evaluate the toxic effects of RD on juveniles of tambaqui (Colossoma macropomum). Our findings show that biomarkers in tambaqui are organ specific and dependent on RD concentration. Alterations in gills structural and respiratory epithelium were followed by changes in hematological parameters such as concentration of hemoglobin, particularly in fish exposed to the higher concentration tested (75% of RD LC<inf>50</inf> 96h). In addition, both RD concentrations affected the biotransformation process in gills of tambaqui negatively. Instead, liver responses suggest that a production of reactive oxygen species (ROS) occurred in fish exposed to RD, particularly in the animals exposed to 75% RD, as seen by imbalances in biotransformation and antioxidant systems. The increased DNA damage observed in red blood cells of tambaqui exposed to RD is in agreement with this hypothesis. Finally, both tested sub-lethal concentrations of RD markedly inhibited the cholinesterase activity in fish brain. Thus, we can suggest that RD is potentially toxic to tambaqui and possibly to other tropical fish species. © 2015 Elsevier Ltd. |
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Braz-Mota, SusanaSadauskas-Henrique, HelenDuarte, Rafael MendonçaVal, Adalberto LuisAlmeida-Val, Vera Maria Fonseca2020-05-20T14:15:53Z2020-05-20T14:15:53Z2015https://repositorio.inpa.gov.br/handle/1/1591110.1016/j.chemosphere.2015.03.042Roundup Original® (RD) is a glyphosate-based herbicide used to control weeds in agriculture. Contamination of Amazon waters has increased as a consequence of anthropogenic pressure, including the use of herbicides as RD. The central goal of this study was to evaluate the toxic effects of RD on juveniles of tambaqui (Colossoma macropomum). Our findings show that biomarkers in tambaqui are organ specific and dependent on RD concentration. Alterations in gills structural and respiratory epithelium were followed by changes in hematological parameters such as concentration of hemoglobin, particularly in fish exposed to the higher concentration tested (75% of RD LC<inf>50</inf> 96h). In addition, both RD concentrations affected the biotransformation process in gills of tambaqui negatively. Instead, liver responses suggest that a production of reactive oxygen species (ROS) occurred in fish exposed to RD, particularly in the animals exposed to 75% RD, as seen by imbalances in biotransformation and antioxidant systems. The increased DNA damage observed in red blood cells of tambaqui exposed to RD is in agreement with this hypothesis. Finally, both tested sub-lethal concentrations of RD markedly inhibited the cholinesterase activity in fish brain. Thus, we can suggest that RD is potentially toxic to tambaqui and possibly to other tropical fish species. © 2015 Elsevier Ltd.Volume 135, Pags. 53-60Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessAquacultureBiomarkersBrainHerbicidesWeed ControlAnthropogenic PressuresBiotransformation ProcessCholinesterase ActivityGlyphosatesHematological ParametersReactive Oxygen SpeciesRespiratory EpitheliumsTambaquiFishAntioxidantBiological MarkerDnaGlucoseGlyphosateHemoglobinReactive Oxygen MetaboliteRoundupUnclassified DrugCholinergic Receptor Stimulating AgentGlycineGlyphosateHerbicideWater PollutantBioaccumulationBiomarkerBiotransformationBrainDnaHerbicidePollution EffectPollution ExposureRiver PollutionTeleostAnimals ExperimentAnimals TissueBiotransformationCholinergic ActivityColossoma MacropomumConcentration (parameters)Controlled StudyDna DamageEnvironmental ExposureErythrocyteGillGlucose Blood LevelHemoglobin DeterminationHistopathologyJuvenile AnimalsLc50LiverNeurologic DiseaseNonhumanRespiratory EpitheliumTeleostToxicity TestingAnalogs And DerivativesAnimalsBrainDna DamageDrug EffectsFishGillLiverMetabolismPhysiologyToxicityWater PollutantAmazon RiverAnimalsiaColossoma MacropomumColossoma MarcopomumTeleosteiAnimalBrainCholinergic AgentsDna DamageFishesGillsGlycineHerbicidesLiverWater Pollutants, ChemicalRoundup® exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macropomuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleChemosphereengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfartigo-inpa.pdfapplication/pdf974815https://repositorio.inpa.gov.br/bitstream/1/15911/1/artigo-inpa.pdffc9722b65b03c9dd06d844f7533a3a39MD511/159112020-05-20 11:04:17.133oai:repositorio:1/15911Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-05-20T15:04:17Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Roundup® exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macropomum |
title |
Roundup® exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macropomum |
spellingShingle |
Roundup® exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macropomum Braz-Mota, Susana Aquaculture Biomarkers Brain Herbicides Weed Control Anthropogenic Pressures Biotransformation Process Cholinesterase Activity Glyphosates Hematological Parameters Reactive Oxygen Species Respiratory Epitheliums Tambaqui Fish Antioxidant Biological Marker Dna Glucose Glyphosate Hemoglobin Reactive Oxygen Metabolite Roundup Unclassified Drug Cholinergic Receptor Stimulating Agent Glycine Glyphosate Herbicide Water Pollutant Bioaccumulation Biomarker Biotransformation Brain Dna Herbicide Pollution Effect Pollution Exposure River Pollution Teleost Animals Experiment Animals Tissue Biotransformation Cholinergic Activity Colossoma Macropomum Concentration (parameters) Controlled Study Dna Damage Environmental Exposure Erythrocyte Gill Glucose Blood Level Hemoglobin Determination Histopathology Juvenile Animals Lc50 Liver Neurologic Disease Nonhuman Respiratory Epithelium Teleost Toxicity Testing Analogs And Derivatives Animals Brain Dna Damage Drug Effects Fish Gill Liver Metabolism Physiology Toxicity Water Pollutant Amazon River Animalsia Colossoma Macropomum Colossoma Marcopomum Teleostei Animal Brain Cholinergic Agents Dna Damage Fishes Gills Glycine Herbicides Liver Water Pollutants, Chemical |
title_short |
Roundup® exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macropomum |
title_full |
Roundup® exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macropomum |
title_fullStr |
Roundup® exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macropomum |
title_full_unstemmed |
Roundup® exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macropomum |
title_sort |
Roundup® exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macropomum |
author |
Braz-Mota, Susana |
author_facet |
Braz-Mota, Susana Sadauskas-Henrique, Helen Duarte, Rafael Mendonça Val, Adalberto Luis Almeida-Val, Vera Maria Fonseca |
author_role |
author |
author2 |
Sadauskas-Henrique, Helen Duarte, Rafael Mendonça Val, Adalberto Luis Almeida-Val, Vera Maria Fonseca |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Braz-Mota, Susana Sadauskas-Henrique, Helen Duarte, Rafael Mendonça Val, Adalberto Luis Almeida-Val, Vera Maria Fonseca |
dc.subject.eng.fl_str_mv |
Aquaculture Biomarkers Brain Herbicides Weed Control Anthropogenic Pressures Biotransformation Process Cholinesterase Activity Glyphosates Hematological Parameters Reactive Oxygen Species Respiratory Epitheliums Tambaqui Fish Antioxidant Biological Marker Dna Glucose Glyphosate Hemoglobin Reactive Oxygen Metabolite Roundup Unclassified Drug Cholinergic Receptor Stimulating Agent Glycine Glyphosate Herbicide Water Pollutant Bioaccumulation Biomarker Biotransformation Brain Dna Herbicide Pollution Effect Pollution Exposure River Pollution Teleost Animals Experiment Animals Tissue Biotransformation Cholinergic Activity Colossoma Macropomum Concentration (parameters) Controlled Study Dna Damage Environmental Exposure Erythrocyte Gill Glucose Blood Level Hemoglobin Determination Histopathology Juvenile Animals Lc50 Liver Neurologic Disease Nonhuman Respiratory Epithelium Teleost Toxicity Testing Analogs And Derivatives Animals Brain Dna Damage Drug Effects Fish Gill Liver Metabolism Physiology Toxicity Water Pollutant Amazon River Animalsia Colossoma Macropomum Colossoma Marcopomum Teleostei Animal Brain Cholinergic Agents Dna Damage Fishes Gills Glycine Herbicides Liver Water Pollutants, Chemical |
topic |
Aquaculture Biomarkers Brain Herbicides Weed Control Anthropogenic Pressures Biotransformation Process Cholinesterase Activity Glyphosates Hematological Parameters Reactive Oxygen Species Respiratory Epitheliums Tambaqui Fish Antioxidant Biological Marker Dna Glucose Glyphosate Hemoglobin Reactive Oxygen Metabolite Roundup Unclassified Drug Cholinergic Receptor Stimulating Agent Glycine Glyphosate Herbicide Water Pollutant Bioaccumulation Biomarker Biotransformation Brain Dna Herbicide Pollution Effect Pollution Exposure River Pollution Teleost Animals Experiment Animals Tissue Biotransformation Cholinergic Activity Colossoma Macropomum Concentration (parameters) Controlled Study Dna Damage Environmental Exposure Erythrocyte Gill Glucose Blood Level Hemoglobin Determination Histopathology Juvenile Animals Lc50 Liver Neurologic Disease Nonhuman Respiratory Epithelium Teleost Toxicity Testing Analogs And Derivatives Animals Brain Dna Damage Drug Effects Fish Gill Liver Metabolism Physiology Toxicity Water Pollutant Amazon River Animalsia Colossoma Macropomum Colossoma Marcopomum Teleostei Animal Brain Cholinergic Agents Dna Damage Fishes Gills Glycine Herbicides Liver Water Pollutants, Chemical |
description |
Roundup Original® (RD) is a glyphosate-based herbicide used to control weeds in agriculture. Contamination of Amazon waters has increased as a consequence of anthropogenic pressure, including the use of herbicides as RD. The central goal of this study was to evaluate the toxic effects of RD on juveniles of tambaqui (Colossoma macropomum). Our findings show that biomarkers in tambaqui are organ specific and dependent on RD concentration. Alterations in gills structural and respiratory epithelium were followed by changes in hematological parameters such as concentration of hemoglobin, particularly in fish exposed to the higher concentration tested (75% of RD LC<inf>50</inf> 96h). In addition, both RD concentrations affected the biotransformation process in gills of tambaqui negatively. Instead, liver responses suggest that a production of reactive oxygen species (ROS) occurred in fish exposed to RD, particularly in the animals exposed to 75% RD, as seen by imbalances in biotransformation and antioxidant systems. The increased DNA damage observed in red blood cells of tambaqui exposed to RD is in agreement with this hypothesis. Finally, both tested sub-lethal concentrations of RD markedly inhibited the cholinesterase activity in fish brain. Thus, we can suggest that RD is potentially toxic to tambaqui and possibly to other tropical fish species. © 2015 Elsevier Ltd. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015 |
dc.date.accessioned.fl_str_mv |
2020-05-20T14:15:53Z |
dc.date.available.fl_str_mv |
2020-05-20T14:15:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/15911 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.chemosphere.2015.03.042 |
url |
https://repositorio.inpa.gov.br/handle/1/15911 |
identifier_str_mv |
10.1016/j.chemosphere.2015.03.042 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 135, Pags. 53-60 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Chemosphere |
publisher.none.fl_str_mv |
Chemosphere |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional do INPA instname:Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
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Instituto Nacional de Pesquisas da Amazônia (INPA) |
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INPA |
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INPA |
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Repositório Institucional do INPA |
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Repositório Institucional do INPA |
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https://repositorio.inpa.gov.br/bitstream/1/15911/1/artigo-inpa.pdf |
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Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA) |
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