Roundup® exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macropomum

Detalhes bibliográficos
Autor(a) principal: Braz-Mota, Susana
Data de Publicação: 2015
Outros Autores: Sadauskas-Henrique, Helen, Duarte, Rafael Mendonça, Val, Adalberto Luis, Almeida-Val, Vera Maria Fonseca
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional do INPA
Texto Completo: https://repositorio.inpa.gov.br/handle/1/15911
Resumo: Roundup Original® (RD) is a glyphosate-based herbicide used to control weeds in agriculture. Contamination of Amazon waters has increased as a consequence of anthropogenic pressure, including the use of herbicides as RD. The central goal of this study was to evaluate the toxic effects of RD on juveniles of tambaqui (Colossoma macropomum). Our findings show that biomarkers in tambaqui are organ specific and dependent on RD concentration. Alterations in gills structural and respiratory epithelium were followed by changes in hematological parameters such as concentration of hemoglobin, particularly in fish exposed to the higher concentration tested (75% of RD LC<inf>50</inf> 96h). In addition, both RD concentrations affected the biotransformation process in gills of tambaqui negatively. Instead, liver responses suggest that a production of reactive oxygen species (ROS) occurred in fish exposed to RD, particularly in the animals exposed to 75% RD, as seen by imbalances in biotransformation and antioxidant systems. The increased DNA damage observed in red blood cells of tambaqui exposed to RD is in agreement with this hypothesis. Finally, both tested sub-lethal concentrations of RD markedly inhibited the cholinesterase activity in fish brain. Thus, we can suggest that RD is potentially toxic to tambaqui and possibly to other tropical fish species. © 2015 Elsevier Ltd.
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spelling Braz-Mota, SusanaSadauskas-Henrique, HelenDuarte, Rafael MendonçaVal, Adalberto LuisAlmeida-Val, Vera Maria Fonseca2020-05-20T14:15:53Z2020-05-20T14:15:53Z2015https://repositorio.inpa.gov.br/handle/1/1591110.1016/j.chemosphere.2015.03.042Roundup Original® (RD) is a glyphosate-based herbicide used to control weeds in agriculture. Contamination of Amazon waters has increased as a consequence of anthropogenic pressure, including the use of herbicides as RD. The central goal of this study was to evaluate the toxic effects of RD on juveniles of tambaqui (Colossoma macropomum). Our findings show that biomarkers in tambaqui are organ specific and dependent on RD concentration. Alterations in gills structural and respiratory epithelium were followed by changes in hematological parameters such as concentration of hemoglobin, particularly in fish exposed to the higher concentration tested (75% of RD LC<inf>50</inf> 96h). In addition, both RD concentrations affected the biotransformation process in gills of tambaqui negatively. Instead, liver responses suggest that a production of reactive oxygen species (ROS) occurred in fish exposed to RD, particularly in the animals exposed to 75% RD, as seen by imbalances in biotransformation and antioxidant systems. The increased DNA damage observed in red blood cells of tambaqui exposed to RD is in agreement with this hypothesis. Finally, both tested sub-lethal concentrations of RD markedly inhibited the cholinesterase activity in fish brain. Thus, we can suggest that RD is potentially toxic to tambaqui and possibly to other tropical fish species. © 2015 Elsevier Ltd.Volume 135, Pags. 53-60Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessAquacultureBiomarkersBrainHerbicidesWeed ControlAnthropogenic PressuresBiotransformation ProcessCholinesterase ActivityGlyphosatesHematological ParametersReactive Oxygen SpeciesRespiratory EpitheliumsTambaquiFishAntioxidantBiological MarkerDnaGlucoseGlyphosateHemoglobinReactive Oxygen MetaboliteRoundupUnclassified DrugCholinergic Receptor Stimulating AgentGlycineGlyphosateHerbicideWater PollutantBioaccumulationBiomarkerBiotransformationBrainDnaHerbicidePollution EffectPollution ExposureRiver PollutionTeleostAnimals ExperimentAnimals TissueBiotransformationCholinergic ActivityColossoma MacropomumConcentration (parameters)Controlled StudyDna DamageEnvironmental ExposureErythrocyteGillGlucose Blood LevelHemoglobin DeterminationHistopathologyJuvenile AnimalsLc50LiverNeurologic DiseaseNonhumanRespiratory EpitheliumTeleostToxicity TestingAnalogs And DerivativesAnimalsBrainDna DamageDrug EffectsFishGillLiverMetabolismPhysiologyToxicityWater PollutantAmazon RiverAnimalsiaColossoma MacropomumColossoma MarcopomumTeleosteiAnimalBrainCholinergic AgentsDna DamageFishesGillsGlycineHerbicidesLiverWater Pollutants, ChemicalRoundup® exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macropomuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleChemosphereengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfartigo-inpa.pdfapplication/pdf974815https://repositorio.inpa.gov.br/bitstream/1/15911/1/artigo-inpa.pdffc9722b65b03c9dd06d844f7533a3a39MD511/159112020-05-20 11:04:17.133oai:repositorio:1/15911Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-05-20T15:04:17Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.en.fl_str_mv Roundup® exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macropomum
title Roundup® exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macropomum
spellingShingle Roundup® exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macropomum
Braz-Mota, Susana
Aquaculture
Biomarkers
Brain
Herbicides
Weed Control
Anthropogenic Pressures
Biotransformation Process
Cholinesterase Activity
Glyphosates
Hematological Parameters
Reactive Oxygen Species
Respiratory Epitheliums
Tambaqui
Fish
Antioxidant
Biological Marker
Dna
Glucose
Glyphosate
Hemoglobin
Reactive Oxygen Metabolite
Roundup
Unclassified Drug
Cholinergic Receptor Stimulating Agent
Glycine
Glyphosate
Herbicide
Water Pollutant
Bioaccumulation
Biomarker
Biotransformation
Brain
Dna
Herbicide
Pollution Effect
Pollution Exposure
River Pollution
Teleost
Animals Experiment
Animals Tissue
Biotransformation
Cholinergic Activity
Colossoma Macropomum
Concentration (parameters)
Controlled Study
Dna Damage
Environmental Exposure
Erythrocyte
Gill
Glucose Blood Level
Hemoglobin Determination
Histopathology
Juvenile Animals
Lc50
Liver
Neurologic Disease
Nonhuman
Respiratory Epithelium
Teleost
Toxicity Testing
Analogs And Derivatives
Animals
Brain
Dna Damage
Drug Effects
Fish
Gill
Liver
Metabolism
Physiology
Toxicity
Water Pollutant
Amazon River
Animalsia
Colossoma Macropomum
Colossoma Marcopomum
Teleostei
Animal
Brain
Cholinergic Agents
Dna Damage
Fishes
Gills
Glycine
Herbicides
Liver
Water Pollutants, Chemical
title_short Roundup® exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macropomum
title_full Roundup® exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macropomum
title_fullStr Roundup® exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macropomum
title_full_unstemmed Roundup® exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macropomum
title_sort Roundup® exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macropomum
author Braz-Mota, Susana
author_facet Braz-Mota, Susana
Sadauskas-Henrique, Helen
Duarte, Rafael Mendonça
Val, Adalberto Luis
Almeida-Val, Vera Maria Fonseca
author_role author
author2 Sadauskas-Henrique, Helen
Duarte, Rafael Mendonça
Val, Adalberto Luis
Almeida-Val, Vera Maria Fonseca
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Braz-Mota, Susana
Sadauskas-Henrique, Helen
Duarte, Rafael Mendonça
Val, Adalberto Luis
Almeida-Val, Vera Maria Fonseca
dc.subject.eng.fl_str_mv Aquaculture
Biomarkers
Brain
Herbicides
Weed Control
Anthropogenic Pressures
Biotransformation Process
Cholinesterase Activity
Glyphosates
Hematological Parameters
Reactive Oxygen Species
Respiratory Epitheliums
Tambaqui
Fish
Antioxidant
Biological Marker
Dna
Glucose
Glyphosate
Hemoglobin
Reactive Oxygen Metabolite
Roundup
Unclassified Drug
Cholinergic Receptor Stimulating Agent
Glycine
Glyphosate
Herbicide
Water Pollutant
Bioaccumulation
Biomarker
Biotransformation
Brain
Dna
Herbicide
Pollution Effect
Pollution Exposure
River Pollution
Teleost
Animals Experiment
Animals Tissue
Biotransformation
Cholinergic Activity
Colossoma Macropomum
Concentration (parameters)
Controlled Study
Dna Damage
Environmental Exposure
Erythrocyte
Gill
Glucose Blood Level
Hemoglobin Determination
Histopathology
Juvenile Animals
Lc50
Liver
Neurologic Disease
Nonhuman
Respiratory Epithelium
Teleost
Toxicity Testing
Analogs And Derivatives
Animals
Brain
Dna Damage
Drug Effects
Fish
Gill
Liver
Metabolism
Physiology
Toxicity
Water Pollutant
Amazon River
Animalsia
Colossoma Macropomum
Colossoma Marcopomum
Teleostei
Animal
Brain
Cholinergic Agents
Dna Damage
Fishes
Gills
Glycine
Herbicides
Liver
Water Pollutants, Chemical
topic Aquaculture
Biomarkers
Brain
Herbicides
Weed Control
Anthropogenic Pressures
Biotransformation Process
Cholinesterase Activity
Glyphosates
Hematological Parameters
Reactive Oxygen Species
Respiratory Epitheliums
Tambaqui
Fish
Antioxidant
Biological Marker
Dna
Glucose
Glyphosate
Hemoglobin
Reactive Oxygen Metabolite
Roundup
Unclassified Drug
Cholinergic Receptor Stimulating Agent
Glycine
Glyphosate
Herbicide
Water Pollutant
Bioaccumulation
Biomarker
Biotransformation
Brain
Dna
Herbicide
Pollution Effect
Pollution Exposure
River Pollution
Teleost
Animals Experiment
Animals Tissue
Biotransformation
Cholinergic Activity
Colossoma Macropomum
Concentration (parameters)
Controlled Study
Dna Damage
Environmental Exposure
Erythrocyte
Gill
Glucose Blood Level
Hemoglobin Determination
Histopathology
Juvenile Animals
Lc50
Liver
Neurologic Disease
Nonhuman
Respiratory Epithelium
Teleost
Toxicity Testing
Analogs And Derivatives
Animals
Brain
Dna Damage
Drug Effects
Fish
Gill
Liver
Metabolism
Physiology
Toxicity
Water Pollutant
Amazon River
Animalsia
Colossoma Macropomum
Colossoma Marcopomum
Teleostei
Animal
Brain
Cholinergic Agents
Dna Damage
Fishes
Gills
Glycine
Herbicides
Liver
Water Pollutants, Chemical
description Roundup Original® (RD) is a glyphosate-based herbicide used to control weeds in agriculture. Contamination of Amazon waters has increased as a consequence of anthropogenic pressure, including the use of herbicides as RD. The central goal of this study was to evaluate the toxic effects of RD on juveniles of tambaqui (Colossoma macropomum). Our findings show that biomarkers in tambaqui are organ specific and dependent on RD concentration. Alterations in gills structural and respiratory epithelium were followed by changes in hematological parameters such as concentration of hemoglobin, particularly in fish exposed to the higher concentration tested (75% of RD LC<inf>50</inf> 96h). In addition, both RD concentrations affected the biotransformation process in gills of tambaqui negatively. Instead, liver responses suggest that a production of reactive oxygen species (ROS) occurred in fish exposed to RD, particularly in the animals exposed to 75% RD, as seen by imbalances in biotransformation and antioxidant systems. The increased DNA damage observed in red blood cells of tambaqui exposed to RD is in agreement with this hypothesis. Finally, both tested sub-lethal concentrations of RD markedly inhibited the cholinesterase activity in fish brain. Thus, we can suggest that RD is potentially toxic to tambaqui and possibly to other tropical fish species. © 2015 Elsevier Ltd.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2020-05-20T14:15:53Z
dc.date.available.fl_str_mv 2020-05-20T14:15:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.inpa.gov.br/handle/1/15911
dc.identifier.doi.none.fl_str_mv 10.1016/j.chemosphere.2015.03.042
url https://repositorio.inpa.gov.br/handle/1/15911
identifier_str_mv 10.1016/j.chemosphere.2015.03.042
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Volume 135, Pags. 53-60
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Chemosphere
publisher.none.fl_str_mv Chemosphere
dc.source.none.fl_str_mv reponame:Repositório Institucional do INPA
instname:Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron:INPA
instname_str Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron_str INPA
institution INPA
reponame_str Repositório Institucional do INPA
collection Repositório Institucional do INPA
bitstream.url.fl_str_mv https://repositorio.inpa.gov.br/bitstream/1/15911/1/artigo-inpa.pdf
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repository.name.fl_str_mv Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)
repository.mail.fl_str_mv
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