In vitro antibiotic and modulatory activity of Mesosphaerum suaveolens (L.) kuntze against candida strains

• Autor(a) principal: Costa, Adrielle Rodrigues
• Data de Publicação: 2020
• Outros Autores: Bezerra, José Weverton Almeida, Cruz, Rafael Pereira da, Freitas, Maria Audilene de, Silva, Viviane Bezerra da, Neto, João Cruz, dos Santos, Antônia Thassya Lucas, Braga, Maria Flaviana Bezerra Morais, Silva, Leomara Andrade da, Rocha, Maria Ivaneide, Kamdem, J. P., Iriti, Marcello, Vitalini, Sara, Duarte, Antônia Eliene, Barros, Luiz Marivando
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional do INPA
• Texto Completo: https://repositorio.inpa.gov.br/handle/1/15458
• topic: Antibiotic Agent; Apigenin; Caffeic Acid; Catechin; Chlorogenic Acid; Ellagic Acid; Flavonoid; Fluconazole; Gallic Acid; Mesosphaerum Suaveolens Extract; Phenol Derivative; Phytochemical; Plant Extract; Quercetin; Rutoside; Unclassified Drug; Anti-fungal Activity; Antifungal Resistance; Anti-microbial Activity; Broth Dilution; Candida; Candida Tropicalis; Cell Viability; Cell Viability Assay; Controlled Study; Disk Diffusion; Drug Activity; Enzyme-linked Immunosorbent Assay; Fungal Strain; Fungus Growth; High Performance Liquid Chromatography; Ic 50; Medicinal Plant; Mesosphaerum Suaveolens; Microbiology; Minimum Fungicidal Concentration; Minimum Inhibitory Concentration; Nonhuman; Phytochemistry; Priority Journal; Retention Time (chromatography); Spectrophotometry; Ultraviolet Spectroscopy

The emergence of fungal resistance to commercial drugs has been a major problem for the WHO. In this context, research with natural products is promising in the discovery of new active substances. Thus, this work evaluated the antifungal effect of a medicinal plant (i.e., Mesosphaerum suaveolens) against strains of the genus Candida, tested the combined effect with the drug fluconazole, and, finally, determined the phenolic constituents present in the species. Initially, aqueous extracts of leaves (AELMs) and aerial parts (AEAPMs) of the species were prepared. For microbiological assays, the minimum fungicidal concentration was determined by broth microdilution, and the combined effect of fluconazole extracts were verified by sub‐inhibitory microdilution concentrations (CFM/8) followed by spectrophotometric readings which were used to determine the IC50. HPLC detected the presence of flavonoids and phenolic acids, detecting eight compounds present in the samples of which caffeic acid and quercetin were major components. The AELMs modulated fluconazole activity since it decreased fluconazole’s IC50 from 7.8 μg/mL to an IC50 of 4.7 μg/mL (CA LM 77) and from 28.8 μg/mL to 18.26 μg/mL (CA INCQS 40006) for the C. albicans strains. The AEAPMs were able to potentiate the effect of fluconazole more effectively than the AELMs. Such an effect was significant for the 16 μg/mL concentration for CA LM 77 and 32 μg/mL for CA INCQS 40006. The AEAPMs as well as the AELMs presented clinically relevant activities for C. tropicalis strains. For the C. tropicalis LM 23 strain, the AEPMs obtained an IC50 of 25 μg/mL and the AELMs an IC50 of 359.9 μg/mL. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.