Evidence of two lineages of the dengue vector Aedes aegypti in the Brazilian Amazon, based on mitochondrial DNA ND4 gene sequences

• Autor(a) principal: Lima, Raimundo Sousa
• Data de Publicação: 2009
• Outros Autores: Scarpassa, Vera Margarete
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional do INPA
• Texto Completo: https://repositorio.inpa.gov.br/handle/1/14956
• topic: Dna, Mitochondrial; Reduced Nicotinamide Adenine Dinucleotide Dehydrogenase; Reduced Nicotinamide Adenine Dinucleotide Dehydrogenase 4; Unclassified Drug; Aedes Aegypti; Brasil; Controlled Study; Dengue; Disease Carrier; Gene Frequency; Gene Sequence; Genetic Distance; Genetic Variability; Geographical Variation (species); Haplotype; Nonhuman; Nucleotide Sequence; Phylogeny; Aedes Aegypti

Genetic variation was estimated in ten samples populations of Aedes aegypti from the Brazilian Amazon, by using a 380 bp fragment of the mitochocondrial NADH dehydrogenase subunit 4 (ND4) gene. A total of 123 individuals were analyzed, whereby 13 haplotypes were found. Mean genetic diversity was slightly high (h = 0.666 ± 0.029; π = 0.0115 ± 0.0010). Two AMOVA analyses indicated that most of the variation (∼70%-72%) occurred within populations. The variation found among and between populations within the groups disclosed lower, but even so, highly significant values. FST values were not significant in most of the comparisons, except for the samples from Pacaraima and Rio Branco. The isolation by distance (IBD) model was not significant (r = 0.2880; p = 0.097) when the samples from Pacaraima and Rio Branco were excluded from the analyses, this indicating that genetic distance is not related to geographic distance. This result may be explained either by passive dispersal patterns (via human migrations and commercial exchange) or be due to the recent expansion of this mosquito in the Brazilian Amazon. Phylogenetic relationship analysis showed two genetically distinct groups (lineages) within the Brazilian Amazon, each sharing haplotypes with populations from West Africa and Asia. Copyright © 2009, Sociedade Brasileira de Genética.