Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/15225 |
Resumo: | The so-called â œ blackwatersâ of the Amazonian Rio Negro are rich in highly coloured dissolved organic carbon (DOC), but ion-poor and very acidic, conditions that would cause fatal ionoregulatory failure in most fish. However these blackwaters support 8% of the worldâ ™ s ichthyofauna. We tested the hypothesis that native DOC provides protection against ionoregulatory dysfunction in this extreme environment. DOCs were isolated by reverse-osmosis from two Rio Negro sites. Physico-chemical characterization clearly indicated a terrigenous origin, with a high proportion of hydroxyl and phenolic sites, high chemical reactivity to protons, and unusual proteinaceous fluorescence. When tested using zebrafish (a model organism), Rio Negro DOC provided almost perfect protection against ionoregulatory disturbances associated with acute exposure to pH 4.0 in ion-poor water. DOC reduced diffusive losses of Na + and Cl â', and promoted a remarkable stimulation of Na + uptake that otherwise would have been completely inhibited. Additionally, prior acclimation to DOC at neutral pH reduced rates of branchial Na + turnover, and provided similar protection against acid-induced ionoregulatory disturbances, even if the DOC was no longer present. These results reinforce the important roles that DOC molecules can play in the regulation of gill functions in freshwater fish, particularly in ion-poor, acidic blackwaters. |
id |
INPA-2_d5bd4731c3c72774c3adf18ed179e0db |
---|---|
oai_identifier_str |
oai:repositorio:1/15225 |
network_acronym_str |
INPA-2 |
network_name_str |
Repositório Institucional do INPA |
repository_id_str |
|
spelling |
Duarte, Rafael MendonçaSmith, Donald ScottVal, Adalberto LuisWood, Chris M.2020-05-07T14:14:50Z2020-05-07T14:14:50Z2016https://repositorio.inpa.gov.br/handle/1/1522510.1038/srep20377The so-called â œ blackwatersâ of the Amazonian Rio Negro are rich in highly coloured dissolved organic carbon (DOC), but ion-poor and very acidic, conditions that would cause fatal ionoregulatory failure in most fish. However these blackwaters support 8% of the worldâ ™ s ichthyofauna. We tested the hypothesis that native DOC provides protection against ionoregulatory dysfunction in this extreme environment. DOCs were isolated by reverse-osmosis from two Rio Negro sites. Physico-chemical characterization clearly indicated a terrigenous origin, with a high proportion of hydroxyl and phenolic sites, high chemical reactivity to protons, and unusual proteinaceous fluorescence. When tested using zebrafish (a model organism), Rio Negro DOC provided almost perfect protection against ionoregulatory disturbances associated with acute exposure to pH 4.0 in ion-poor water. DOC reduced diffusive losses of Na + and Cl â', and promoted a remarkable stimulation of Na + uptake that otherwise would have been completely inhibited. Additionally, prior acclimation to DOC at neutral pH reduced rates of branchial Na + turnover, and provided similar protection against acid-induced ionoregulatory disturbances, even if the DOC was no longer present. These results reinforce the important roles that DOC molecules can play in the regulation of gill functions in freshwater fish, particularly in ion-poor, acidic blackwaters.Volume 6Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessCalciumCarbonIonSodiumAnimalsBrasilChemistryGrowth, Development And AgingPhStress, PhysiologicalRiverZebrafishAnimalBrasilCalciumCarbonHydrogen-ion ConcentrationIonsRiversSodiumStress, PhysiologicalZebrafishDissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposureinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleScientific Reportsengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfapplication/pdf1018315https://repositorio.inpa.gov.br/bitstream/1/15225/1/artigo-inpa.pdf09dc9ec74b923572d66147c0927730c4MD51CC-LICENSElicense_rdfapplication/octet-stream914https://repositorio.inpa.gov.br/bitstream/1/15225/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD521/152252020-07-14 10:59:38.409oai:repositorio:1/15225Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T14:59:38Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure |
title |
Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure |
spellingShingle |
Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure Duarte, Rafael Mendonça Calcium Carbon Ion Sodium Animals Brasil Chemistry Growth, Development And Aging Ph Stress, Physiological River Zebrafish Animal Brasil Calcium Carbon Hydrogen-ion Concentration Ions Rivers Sodium Stress, Physiological Zebrafish |
title_short |
Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure |
title_full |
Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure |
title_fullStr |
Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure |
title_full_unstemmed |
Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure |
title_sort |
Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure |
author |
Duarte, Rafael Mendonça |
author_facet |
Duarte, Rafael Mendonça Smith, Donald Scott Val, Adalberto Luis Wood, Chris M. |
author_role |
author |
author2 |
Smith, Donald Scott Val, Adalberto Luis Wood, Chris M. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Duarte, Rafael Mendonça Smith, Donald Scott Val, Adalberto Luis Wood, Chris M. |
dc.subject.eng.fl_str_mv |
Calcium Carbon Ion Sodium Animals Brasil Chemistry Growth, Development And Aging Ph Stress, Physiological River Zebrafish Animal Brasil Calcium Carbon Hydrogen-ion Concentration Ions Rivers Sodium Stress, Physiological Zebrafish |
topic |
Calcium Carbon Ion Sodium Animals Brasil Chemistry Growth, Development And Aging Ph Stress, Physiological River Zebrafish Animal Brasil Calcium Carbon Hydrogen-ion Concentration Ions Rivers Sodium Stress, Physiological Zebrafish |
description |
The so-called â œ blackwatersâ of the Amazonian Rio Negro are rich in highly coloured dissolved organic carbon (DOC), but ion-poor and very acidic, conditions that would cause fatal ionoregulatory failure in most fish. However these blackwaters support 8% of the worldâ ™ s ichthyofauna. We tested the hypothesis that native DOC provides protection against ionoregulatory dysfunction in this extreme environment. DOCs were isolated by reverse-osmosis from two Rio Negro sites. Physico-chemical characterization clearly indicated a terrigenous origin, with a high proportion of hydroxyl and phenolic sites, high chemical reactivity to protons, and unusual proteinaceous fluorescence. When tested using zebrafish (a model organism), Rio Negro DOC provided almost perfect protection against ionoregulatory disturbances associated with acute exposure to pH 4.0 in ion-poor water. DOC reduced diffusive losses of Na + and Cl â', and promoted a remarkable stimulation of Na + uptake that otherwise would have been completely inhibited. Additionally, prior acclimation to DOC at neutral pH reduced rates of branchial Na + turnover, and provided similar protection against acid-induced ionoregulatory disturbances, even if the DOC was no longer present. These results reinforce the important roles that DOC molecules can play in the regulation of gill functions in freshwater fish, particularly in ion-poor, acidic blackwaters. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2020-05-07T14:14:50Z |
dc.date.available.fl_str_mv |
2020-05-07T14:14:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/15225 |
dc.identifier.doi.none.fl_str_mv |
10.1038/srep20377 |
url |
https://repositorio.inpa.gov.br/handle/1/15225 |
identifier_str_mv |
10.1038/srep20377 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 6 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Scientific Reports |
publisher.none.fl_str_mv |
Scientific Reports |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional do INPA instname:Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
instname_str |
Instituto Nacional de Pesquisas da Amazônia (INPA) |
instacron_str |
INPA |
institution |
INPA |
reponame_str |
Repositório Institucional do INPA |
collection |
Repositório Institucional do INPA |
bitstream.url.fl_str_mv |
https://repositorio.inpa.gov.br/bitstream/1/15225/1/artigo-inpa.pdf https://repositorio.inpa.gov.br/bitstream/1/15225/2/license_rdf |
bitstream.checksum.fl_str_mv |
09dc9ec74b923572d66147c0927730c4 4d2950bda3d176f570a9f8b328dfbbef |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA) |
repository.mail.fl_str_mv |
|
_version_ |
1809928891873099776 |