Lipase activity among bacteria isolated from amazonian soils

Detalhes bibliográficos
Autor(a) principal: Willerding, André Luis
Data de Publicação: 2011
Outros Autores: Oliveira, Luiz Antonio de, Moreira, Francisco Wesen, Germano, Mariana Gomes, Chagas Júnior, A. F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional do INPA
Texto Completo: https://repositorio.inpa.gov.br/handle/1/16161
Resumo: The objective of this study was to select lipase-producing bacteria collected from different counties of the Amazon region. Of the 440 bacteria strains, 181 were selected for the lipase assay in qualitative tests at Petri dishes, being 75 (41) lipase positive. The enzymatic index was determined during fifteen days at different temperatures (30°, 35°, 40°, and 45°C). The highest lipase activity was observed within 72 hours at 30°C. Twelve bacteria strains presented an index equal to or greater than the standard used like reference, demonstrating the potential of microbial resource. After the bioassay in Petri dishes, the selected bacteria strains were analyzed in quantitative tests on p-nitrophenyl palmitate (p-NPP). A group of the strains was selected for other phases of study with the use in oleaginous substrates of the Amazonian flora, aiming for the application in processes like oil biotransformation. © 2011 André Luis Willerding et al.
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spelling Willerding, André LuisOliveira, Luiz Antonio deMoreira, Francisco WesenGermano, Mariana GomesChagas Júnior, A. F.2020-05-25T19:10:49Z2020-05-25T19:10:49Z2011https://repositorio.inpa.gov.br/handle/1/1616110.4061/2011/720194The objective of this study was to select lipase-producing bacteria collected from different counties of the Amazon region. Of the 440 bacteria strains, 181 were selected for the lipase assay in qualitative tests at Petri dishes, being 75 (41) lipase positive. The enzymatic index was determined during fifteen days at different temperatures (30°, 35°, 40°, and 45°C). The highest lipase activity was observed within 72 hours at 30°C. Twelve bacteria strains presented an index equal to or greater than the standard used like reference, demonstrating the potential of microbial resource. After the bioassay in Petri dishes, the selected bacteria strains were analyzed in quantitative tests on p-nitrophenyl palmitate (p-NPP). A group of the strains was selected for other phases of study with the use in oleaginous substrates of the Amazonian flora, aiming for the application in processes like oil biotransformation. © 2011 André Luis Willerding et al.Volume 2011, Número 1Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccess4 NitrophenylpalmitatePalmitic Acid DerivativeTriacylglycerol LipaseUnclassified DrugBacterial StrainBacterium IsolationBiotransformationBrasilBurkholderiaEnzyme ActivityEnzyme AssayEnzyme SynthesisMicrofloraNonhumanPetri DishPriority JournalPseudomonasQualitative AnalysisSoil MicrofloraTemperatureLipase activity among bacteria isolated from amazonian soilsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleEnzyme Researchengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfartigo-inpa.pdfapplication/pdf1288593https://repositorio.inpa.gov.br/bitstream/1/16161/1/artigo-inpa.pdf820599d88bd9c525d98ed63496a9f308MD511/161612020-05-25 16:29:14.745oai:repositorio:1/16161Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-05-25T20:29:14Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.en.fl_str_mv Lipase activity among bacteria isolated from amazonian soils
title Lipase activity among bacteria isolated from amazonian soils
spellingShingle Lipase activity among bacteria isolated from amazonian soils
Willerding, André Luis
4 Nitrophenylpalmitate
Palmitic Acid Derivative
Triacylglycerol Lipase
Unclassified Drug
Bacterial Strain
Bacterium Isolation
Biotransformation
Brasil
Burkholderia
Enzyme Activity
Enzyme Assay
Enzyme Synthesis
Microflora
Nonhuman
Petri Dish
Priority Journal
Pseudomonas
Qualitative Analysis
Soil Microflora
Temperature
title_short Lipase activity among bacteria isolated from amazonian soils
title_full Lipase activity among bacteria isolated from amazonian soils
title_fullStr Lipase activity among bacteria isolated from amazonian soils
title_full_unstemmed Lipase activity among bacteria isolated from amazonian soils
title_sort Lipase activity among bacteria isolated from amazonian soils
author Willerding, André Luis
author_facet Willerding, André Luis
Oliveira, Luiz Antonio de
Moreira, Francisco Wesen
Germano, Mariana Gomes
Chagas Júnior, A. F.
author_role author
author2 Oliveira, Luiz Antonio de
Moreira, Francisco Wesen
Germano, Mariana Gomes
Chagas Júnior, A. F.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Willerding, André Luis
Oliveira, Luiz Antonio de
Moreira, Francisco Wesen
Germano, Mariana Gomes
Chagas Júnior, A. F.
dc.subject.eng.fl_str_mv 4 Nitrophenylpalmitate
Palmitic Acid Derivative
Triacylglycerol Lipase
Unclassified Drug
Bacterial Strain
Bacterium Isolation
Biotransformation
Brasil
Burkholderia
Enzyme Activity
Enzyme Assay
Enzyme Synthesis
Microflora
Nonhuman
Petri Dish
Priority Journal
Pseudomonas
Qualitative Analysis
Soil Microflora
Temperature
topic 4 Nitrophenylpalmitate
Palmitic Acid Derivative
Triacylglycerol Lipase
Unclassified Drug
Bacterial Strain
Bacterium Isolation
Biotransformation
Brasil
Burkholderia
Enzyme Activity
Enzyme Assay
Enzyme Synthesis
Microflora
Nonhuman
Petri Dish
Priority Journal
Pseudomonas
Qualitative Analysis
Soil Microflora
Temperature
description The objective of this study was to select lipase-producing bacteria collected from different counties of the Amazon region. Of the 440 bacteria strains, 181 were selected for the lipase assay in qualitative tests at Petri dishes, being 75 (41) lipase positive. The enzymatic index was determined during fifteen days at different temperatures (30°, 35°, 40°, and 45°C). The highest lipase activity was observed within 72 hours at 30°C. Twelve bacteria strains presented an index equal to or greater than the standard used like reference, demonstrating the potential of microbial resource. After the bioassay in Petri dishes, the selected bacteria strains were analyzed in quantitative tests on p-nitrophenyl palmitate (p-NPP). A group of the strains was selected for other phases of study with the use in oleaginous substrates of the Amazonian flora, aiming for the application in processes like oil biotransformation. © 2011 André Luis Willerding et al.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2020-05-25T19:10:49Z
dc.date.available.fl_str_mv 2020-05-25T19:10:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.inpa.gov.br/handle/1/16161
dc.identifier.doi.none.fl_str_mv 10.4061/2011/720194
url https://repositorio.inpa.gov.br/handle/1/16161
identifier_str_mv 10.4061/2011/720194
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Volume 2011, Número 1
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Enzyme Research
publisher.none.fl_str_mv Enzyme Research
dc.source.none.fl_str_mv reponame:Repositório Institucional do INPA
instname:Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron:INPA
instname_str Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron_str INPA
institution INPA
reponame_str Repositório Institucional do INPA
collection Repositório Institucional do INPA
bitstream.url.fl_str_mv https://repositorio.inpa.gov.br/bitstream/1/16161/1/artigo-inpa.pdf
bitstream.checksum.fl_str_mv 820599d88bd9c525d98ed63496a9f308
bitstream.checksumAlgorithm.fl_str_mv MD5
repository.name.fl_str_mv Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)
repository.mail.fl_str_mv
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