Intense genomic reorganization in the genus Oecomys (Rodentia, Sigmodontinae): Comparison between DNA barcoding and mapping of repetitive elements in three species of the Brazilian Amazon
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/15855 |
Resumo: | Oecomys Thomas, 1906 is one of the most diverse and widely distributed genera within the tribe Oryzomyini. At least sixteen species in this genus have been described to date, but it is believed this genus contains undescribed species. Morphological, molecular and cytogenetic study has revealed an uncertain taxonomic status for several Oecomys species, suggesting the presence of a complex of species. The present work had the goal of contributing to the genetic characterization of the genus Oecomys in the Brazilian Amazon. Thirty specimens were collected from four locations in the Brazilian Amazon and three nominal species recognized: Oecomys auyantepui (Tate, 1939), O. bicolor (Tomes, 1860) and O. rutilus (Anthony, 1921). COI sequence analysis grouped O. auyantepui, O. bicolor and O. rutilus specimens into one, three and two clades, respectively, which is consistent with their geographic distribution. Cytogenetic data for O. auyantepui revealed the sympatric occurrence of two different diploid numbers, 2n=64/NFa=110 and 2n=66/NFa=114, suggesting polymorphism while O. bicolor exhibited 2n=80/NFa=142 and O. rutilus 2n=54/NFa=90. The distribution of constitutive heterochromatin followed a species-specific pattern. Interspecific variation was evident in the chromosomal location and number of 18S rDNA loci. However, not all loci showed signs of activity. All three species displayed a similar pattern for 5S rDNA, with only one pair carrying this locus. Interstitial telomeric sites were found only in O. auyantepui. The data presented in this work reinforce intra- and interspecific variations observed in the diploid number of Oecomys species and indicate that chromosomal rearrangements have led to the appearance of different diploid numbers and karyotypic formulas. © Renan Gabriel Gomes Júnior et al. |
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Gomes Júnior, Renan GabrielSchneider, Carlos HenriqueLira, Thatianna deCarvalho, Natália Dayane MouraFeldberg, ElianaSilva, Maria Nazareth Ferreira daGross, Maria Claudia2020-05-19T19:58:41Z2020-05-19T19:58:41Z2016https://repositorio.inpa.gov.br/handle/1/1585510.3897/compcytogen.v10i3.8306Oecomys Thomas, 1906 is one of the most diverse and widely distributed genera within the tribe Oryzomyini. At least sixteen species in this genus have been described to date, but it is believed this genus contains undescribed species. Morphological, molecular and cytogenetic study has revealed an uncertain taxonomic status for several Oecomys species, suggesting the presence of a complex of species. The present work had the goal of contributing to the genetic characterization of the genus Oecomys in the Brazilian Amazon. Thirty specimens were collected from four locations in the Brazilian Amazon and three nominal species recognized: Oecomys auyantepui (Tate, 1939), O. bicolor (Tomes, 1860) and O. rutilus (Anthony, 1921). COI sequence analysis grouped O. auyantepui, O. bicolor and O. rutilus specimens into one, three and two clades, respectively, which is consistent with their geographic distribution. Cytogenetic data for O. auyantepui revealed the sympatric occurrence of two different diploid numbers, 2n=64/NFa=110 and 2n=66/NFa=114, suggesting polymorphism while O. bicolor exhibited 2n=80/NFa=142 and O. rutilus 2n=54/NFa=90. The distribution of constitutive heterochromatin followed a species-specific pattern. Interspecific variation was evident in the chromosomal location and number of 18S rDNA loci. However, not all loci showed signs of activity. All three species displayed a similar pattern for 5S rDNA, with only one pair carrying this locus. Interstitial telomeric sites were found only in O. auyantepui. The data presented in this work reinforce intra- and interspecific variations observed in the diploid number of Oecomys species and indicate that chromosomal rearrangements have led to the appearance of different diploid numbers and karyotypic formulas. © Renan Gabriel Gomes Júnior et al.Volume 10, Número 3, Pags. 401-426Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessIntense genomic reorganization in the genus Oecomys (Rodentia, Sigmodontinae): Comparison between DNA barcoding and mapping of repetitive elements in three species of the Brazilian Amazoninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleComparative Cytogeneticsengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa5.pdfartigo-inpa5.pdfapplication/pdf831224https://repositorio.inpa.gov.br/bitstream/1/15855/1/artigo-inpa5.pdf6cc53f047cebd302d372e2e51bdfc79aMD511/158552020-05-27 18:01:36.36oai:repositorio:1/15855Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-05-27T22:01:36Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Intense genomic reorganization in the genus Oecomys (Rodentia, Sigmodontinae): Comparison between DNA barcoding and mapping of repetitive elements in three species of the Brazilian Amazon |
title |
Intense genomic reorganization in the genus Oecomys (Rodentia, Sigmodontinae): Comparison between DNA barcoding and mapping of repetitive elements in three species of the Brazilian Amazon |
spellingShingle |
Intense genomic reorganization in the genus Oecomys (Rodentia, Sigmodontinae): Comparison between DNA barcoding and mapping of repetitive elements in three species of the Brazilian Amazon Gomes Júnior, Renan Gabriel |
title_short |
Intense genomic reorganization in the genus Oecomys (Rodentia, Sigmodontinae): Comparison between DNA barcoding and mapping of repetitive elements in three species of the Brazilian Amazon |
title_full |
Intense genomic reorganization in the genus Oecomys (Rodentia, Sigmodontinae): Comparison between DNA barcoding and mapping of repetitive elements in three species of the Brazilian Amazon |
title_fullStr |
Intense genomic reorganization in the genus Oecomys (Rodentia, Sigmodontinae): Comparison between DNA barcoding and mapping of repetitive elements in three species of the Brazilian Amazon |
title_full_unstemmed |
Intense genomic reorganization in the genus Oecomys (Rodentia, Sigmodontinae): Comparison between DNA barcoding and mapping of repetitive elements in three species of the Brazilian Amazon |
title_sort |
Intense genomic reorganization in the genus Oecomys (Rodentia, Sigmodontinae): Comparison between DNA barcoding and mapping of repetitive elements in three species of the Brazilian Amazon |
author |
Gomes Júnior, Renan Gabriel |
author_facet |
Gomes Júnior, Renan Gabriel Schneider, Carlos Henrique Lira, Thatianna de Carvalho, Natália Dayane Moura Feldberg, Eliana Silva, Maria Nazareth Ferreira da Gross, Maria Claudia |
author_role |
author |
author2 |
Schneider, Carlos Henrique Lira, Thatianna de Carvalho, Natália Dayane Moura Feldberg, Eliana Silva, Maria Nazareth Ferreira da Gross, Maria Claudia |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Gomes Júnior, Renan Gabriel Schneider, Carlos Henrique Lira, Thatianna de Carvalho, Natália Dayane Moura Feldberg, Eliana Silva, Maria Nazareth Ferreira da Gross, Maria Claudia |
description |
Oecomys Thomas, 1906 is one of the most diverse and widely distributed genera within the tribe Oryzomyini. At least sixteen species in this genus have been described to date, but it is believed this genus contains undescribed species. Morphological, molecular and cytogenetic study has revealed an uncertain taxonomic status for several Oecomys species, suggesting the presence of a complex of species. The present work had the goal of contributing to the genetic characterization of the genus Oecomys in the Brazilian Amazon. Thirty specimens were collected from four locations in the Brazilian Amazon and three nominal species recognized: Oecomys auyantepui (Tate, 1939), O. bicolor (Tomes, 1860) and O. rutilus (Anthony, 1921). COI sequence analysis grouped O. auyantepui, O. bicolor and O. rutilus specimens into one, three and two clades, respectively, which is consistent with their geographic distribution. Cytogenetic data for O. auyantepui revealed the sympatric occurrence of two different diploid numbers, 2n=64/NFa=110 and 2n=66/NFa=114, suggesting polymorphism while O. bicolor exhibited 2n=80/NFa=142 and O. rutilus 2n=54/NFa=90. The distribution of constitutive heterochromatin followed a species-specific pattern. Interspecific variation was evident in the chromosomal location and number of 18S rDNA loci. However, not all loci showed signs of activity. All three species displayed a similar pattern for 5S rDNA, with only one pair carrying this locus. Interstitial telomeric sites were found only in O. auyantepui. The data presented in this work reinforce intra- and interspecific variations observed in the diploid number of Oecomys species and indicate that chromosomal rearrangements have led to the appearance of different diploid numbers and karyotypic formulas. © Renan Gabriel Gomes Júnior et al. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2020-05-19T19:58:41Z |
dc.date.available.fl_str_mv |
2020-05-19T19:58:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/15855 |
dc.identifier.doi.none.fl_str_mv |
10.3897/compcytogen.v10i3.8306 |
url |
https://repositorio.inpa.gov.br/handle/1/15855 |
identifier_str_mv |
10.3897/compcytogen.v10i3.8306 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 10, Número 3, Pags. 401-426 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Comparative Cytogenetics |
publisher.none.fl_str_mv |
Comparative Cytogenetics |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional do INPA instname:Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
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Repositório Institucional do INPA |
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https://repositorio.inpa.gov.br/bitstream/1/15855/1/artigo-inpa5.pdf |
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