Anti-Anopheles darlingi saliva antibodies as marker of Plasmodium vivax infection and clinical immunity in the Brazilian Amazon
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/16305 |
Resumo: | Background. Despite governmental and private efforts on providing malaria control, this disease continues to be a major health threat. Thus, innovative strategies are needed to reduce disease burden. The malaria vectors, through the injection of saliva into the host skin, play important role on disease transmission and may influence malaria morbidity. This study describes the humoral immune response against Anopheles (An.) darlingi saliva in volunteers from the Brazilian Amazon and addresses the association between levels of specific antibodies and clinical presentation of Plasmodium (P.) vivax infection. Methods. Adult volunteers from communities in the Rondônia State, Brazil, were screened in order to assess the presence of P. vivax infection by light microscopy and nested PCR. Non-infected volunteers and individuals with symptomatic or symptomless infection were randomly selected and plasma collected. An. darlingi salivary gland sonicates (SGS) were prepared and used to measure anti-saliva antibody levels. Plasma interleukin (IL)-10 and interferon (IFN)- levels were also estimated and correlated to anti-SGS levels. Results. Individuals infected with P. vivax presented higher levels of anti-SGS than non-infected individuals and antibody levels could discriminate infection. Furthermore, anti-saliva antibody measurement was also useful to distinguish asymptomatic infection from non-infection, with a high likelihood ratio. Interestingly, individuals with asymptomatic parasitaemia presented higher titers of anti-SGS and lower IFN-/IL-10 ratio than symptomatic ones. In P. vivax-infected asymptomatic individuals, the IFN-/IL-10 ratio was inversely correlated to anti-SGS titers, although not for while in symptomatic volunteers. Conclusion. The estimation of anti-An. darlingi antibody levels can indicate the probable P. vivax infection status and also could serve as a marker of disease severity in this region of Brazilian Amazon. © 2009 Andrade et al; licensee BioMed Central Ltd. |
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Andrade, Bruno B.Rocha, Bruno CoelhoReis-Filho, AntonioCamargo, Luis Marcelo AranhaTadei, Wanderli PedroMoreira, Luciano AndradeBarral, Aldina Maria PradoBarral-Netto, M.2020-06-03T19:27:18Z2020-06-03T19:27:18Z2009https://repositorio.inpa.gov.br/handle/1/1630510.1186/1475-2875-8-121Background. Despite governmental and private efforts on providing malaria control, this disease continues to be a major health threat. Thus, innovative strategies are needed to reduce disease burden. The malaria vectors, through the injection of saliva into the host skin, play important role on disease transmission and may influence malaria morbidity. This study describes the humoral immune response against Anopheles (An.) darlingi saliva in volunteers from the Brazilian Amazon and addresses the association between levels of specific antibodies and clinical presentation of Plasmodium (P.) vivax infection. Methods. Adult volunteers from communities in the Rondônia State, Brazil, were screened in order to assess the presence of P. vivax infection by light microscopy and nested PCR. Non-infected volunteers and individuals with symptomatic or symptomless infection were randomly selected and plasma collected. An. darlingi salivary gland sonicates (SGS) were prepared and used to measure anti-saliva antibody levels. Plasma interleukin (IL)-10 and interferon (IFN)- levels were also estimated and correlated to anti-SGS levels. Results. Individuals infected with P. vivax presented higher levels of anti-SGS than non-infected individuals and antibody levels could discriminate infection. Furthermore, anti-saliva antibody measurement was also useful to distinguish asymptomatic infection from non-infection, with a high likelihood ratio. Interestingly, individuals with asymptomatic parasitaemia presented higher titers of anti-SGS and lower IFN-/IL-10 ratio than symptomatic ones. In P. vivax-infected asymptomatic individuals, the IFN-/IL-10 ratio was inversely correlated to anti-SGS titers, although not for while in symptomatic volunteers. Conclusion. The estimation of anti-An. darlingi antibody levels can indicate the probable P. vivax infection status and also could serve as a marker of disease severity in this region of Brazilian Amazon. © 2009 Andrade et al; licensee BioMed Central Ltd.Volume 8, Número 1Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessDarlingi Saliva Gland Sonicate AntibodyGamma InterferonInterleukin-10Protozoon AntibodyUnclassified DrugAntibodyBiological MarkerGamma InterferonInsect ProteinsInterleukin-10AdultAntibody Blood LevelAntibody TiterBlood SamplingBrasilConcentration (parameters)Control GroupControlled StudyCytokine ProductionDisease SeverityFemaleHumanHumoral ImmunityImmunityMajor Clinical StudyMicroscopyParasitemiaPlasmodium VivaxPlasmodium Vivax MalariaPolymerase Chain ReactionScreeningAnimalsAnophelesBloodImmunologyMiddle AgedPlasmodium Vivax MalariaPrediction And ForecastingSalivaAdultAnimalAnophelesAntibodiesBiological MarkersBrasilFemaleHumansInsect ProteinsInterferon-gammaInterleukin-10Malaria, VivaxMiddle AgedPredictive Value Of TestsSalivaYoung AdultAnti-Anopheles darlingi saliva antibodies as marker of Plasmodium vivax infection and clinical immunity in the Brazilian Amazoninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMalaria Journalengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfartigo-inpa.pdfapplication/pdf262208https://repositorio.inpa.gov.br/bitstream/1/16305/1/artigo-inpa.pdf4e0b2aa4ddabd6b20eb0f200ff54a629MD511/163052020-06-03 15:52:35.186oai:repositorio:1/16305Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-06-03T19:52:35Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Anti-Anopheles darlingi saliva antibodies as marker of Plasmodium vivax infection and clinical immunity in the Brazilian Amazon |
title |
Anti-Anopheles darlingi saliva antibodies as marker of Plasmodium vivax infection and clinical immunity in the Brazilian Amazon |
spellingShingle |
Anti-Anopheles darlingi saliva antibodies as marker of Plasmodium vivax infection and clinical immunity in the Brazilian Amazon Andrade, Bruno B. Darlingi Saliva Gland Sonicate Antibody Gamma Interferon Interleukin-10 Protozoon Antibody Unclassified Drug Antibody Biological Marker Gamma Interferon Insect Proteins Interleukin-10 Adult Antibody Blood Level Antibody Titer Blood Sampling Brasil Concentration (parameters) Control Group Controlled Study Cytokine Production Disease Severity Female Human Humoral Immunity Immunity Major Clinical Study Microscopy Parasitemia Plasmodium Vivax Plasmodium Vivax Malaria Polymerase Chain Reaction Screening Animals Anopheles Blood Immunology Middle Aged Plasmodium Vivax Malaria Prediction And Forecasting Saliva Adult Animal Anopheles Antibodies Biological Markers Brasil Female Humans Insect Proteins Interferon-gamma Interleukin-10 Malaria, Vivax Middle Aged Predictive Value Of Tests Saliva Young Adult |
title_short |
Anti-Anopheles darlingi saliva antibodies as marker of Plasmodium vivax infection and clinical immunity in the Brazilian Amazon |
title_full |
Anti-Anopheles darlingi saliva antibodies as marker of Plasmodium vivax infection and clinical immunity in the Brazilian Amazon |
title_fullStr |
Anti-Anopheles darlingi saliva antibodies as marker of Plasmodium vivax infection and clinical immunity in the Brazilian Amazon |
title_full_unstemmed |
Anti-Anopheles darlingi saliva antibodies as marker of Plasmodium vivax infection and clinical immunity in the Brazilian Amazon |
title_sort |
Anti-Anopheles darlingi saliva antibodies as marker of Plasmodium vivax infection and clinical immunity in the Brazilian Amazon |
author |
Andrade, Bruno B. |
author_facet |
Andrade, Bruno B. Rocha, Bruno Coelho Reis-Filho, Antonio Camargo, Luis Marcelo Aranha Tadei, Wanderli Pedro Moreira, Luciano Andrade Barral, Aldina Maria Prado Barral-Netto, M. |
author_role |
author |
author2 |
Rocha, Bruno Coelho Reis-Filho, Antonio Camargo, Luis Marcelo Aranha Tadei, Wanderli Pedro Moreira, Luciano Andrade Barral, Aldina Maria Prado Barral-Netto, M. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Andrade, Bruno B. Rocha, Bruno Coelho Reis-Filho, Antonio Camargo, Luis Marcelo Aranha Tadei, Wanderli Pedro Moreira, Luciano Andrade Barral, Aldina Maria Prado Barral-Netto, M. |
dc.subject.eng.fl_str_mv |
Darlingi Saliva Gland Sonicate Antibody Gamma Interferon Interleukin-10 Protozoon Antibody Unclassified Drug Antibody Biological Marker Gamma Interferon Insect Proteins Interleukin-10 Adult Antibody Blood Level Antibody Titer Blood Sampling Brasil Concentration (parameters) Control Group Controlled Study Cytokine Production Disease Severity Female Human Humoral Immunity Immunity Major Clinical Study Microscopy Parasitemia Plasmodium Vivax Plasmodium Vivax Malaria Polymerase Chain Reaction Screening Animals Anopheles Blood Immunology Middle Aged Plasmodium Vivax Malaria Prediction And Forecasting Saliva Adult Animal Anopheles Antibodies Biological Markers Brasil Female Humans Insect Proteins Interferon-gamma Interleukin-10 Malaria, Vivax Middle Aged Predictive Value Of Tests Saliva Young Adult |
topic |
Darlingi Saliva Gland Sonicate Antibody Gamma Interferon Interleukin-10 Protozoon Antibody Unclassified Drug Antibody Biological Marker Gamma Interferon Insect Proteins Interleukin-10 Adult Antibody Blood Level Antibody Titer Blood Sampling Brasil Concentration (parameters) Control Group Controlled Study Cytokine Production Disease Severity Female Human Humoral Immunity Immunity Major Clinical Study Microscopy Parasitemia Plasmodium Vivax Plasmodium Vivax Malaria Polymerase Chain Reaction Screening Animals Anopheles Blood Immunology Middle Aged Plasmodium Vivax Malaria Prediction And Forecasting Saliva Adult Animal Anopheles Antibodies Biological Markers Brasil Female Humans Insect Proteins Interferon-gamma Interleukin-10 Malaria, Vivax Middle Aged Predictive Value Of Tests Saliva Young Adult |
description |
Background. Despite governmental and private efforts on providing malaria control, this disease continues to be a major health threat. Thus, innovative strategies are needed to reduce disease burden. The malaria vectors, through the injection of saliva into the host skin, play important role on disease transmission and may influence malaria morbidity. This study describes the humoral immune response against Anopheles (An.) darlingi saliva in volunteers from the Brazilian Amazon and addresses the association between levels of specific antibodies and clinical presentation of Plasmodium (P.) vivax infection. Methods. Adult volunteers from communities in the Rondônia State, Brazil, were screened in order to assess the presence of P. vivax infection by light microscopy and nested PCR. Non-infected volunteers and individuals with symptomatic or symptomless infection were randomly selected and plasma collected. An. darlingi salivary gland sonicates (SGS) were prepared and used to measure anti-saliva antibody levels. Plasma interleukin (IL)-10 and interferon (IFN)- levels were also estimated and correlated to anti-SGS levels. Results. Individuals infected with P. vivax presented higher levels of anti-SGS than non-infected individuals and antibody levels could discriminate infection. Furthermore, anti-saliva antibody measurement was also useful to distinguish asymptomatic infection from non-infection, with a high likelihood ratio. Interestingly, individuals with asymptomatic parasitaemia presented higher titers of anti-SGS and lower IFN-/IL-10 ratio than symptomatic ones. In P. vivax-infected asymptomatic individuals, the IFN-/IL-10 ratio was inversely correlated to anti-SGS titers, although not for while in symptomatic volunteers. Conclusion. The estimation of anti-An. darlingi antibody levels can indicate the probable P. vivax infection status and also could serve as a marker of disease severity in this region of Brazilian Amazon. © 2009 Andrade et al; licensee BioMed Central Ltd. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009 |
dc.date.accessioned.fl_str_mv |
2020-06-03T19:27:18Z |
dc.date.available.fl_str_mv |
2020-06-03T19:27:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/16305 |
dc.identifier.doi.none.fl_str_mv |
10.1186/1475-2875-8-121 |
url |
https://repositorio.inpa.gov.br/handle/1/16305 |
identifier_str_mv |
10.1186/1475-2875-8-121 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 8, Número 1 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
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openAccess |
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Malaria Journal |
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Malaria Journal |
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Repositório Institucional do INPA |
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