Genetic diversity in dengue mosquito, Aedes aegypti (Diptera: Culicidae) from Amazon region: Comparative analysis with isozymes and RAPD loci
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/16174 |
Resumo: | Four populations of Aedes aegypti from Manaus were studied, using allozymes and RAPD loci, to determine intra- and interpopulation genetic variability and differentiation and to compare genetic structure parameters assessed with both markers. Five RAPD primers produced 52 polymorphic fragments, whereas only seven of 18 isozyme loci were polymorphic. The population from Praça 14 was the most polymorphic (P= 94.23% and P= 55.6%); while those from Coroado (P= 82.69% and P= 44.40%) and from Cidade Nova (P= 84.61% and P= 44.40%) were the least polymorphic, for both RAPD and isozymes respectively. The observed heterozygosity was higher between populations (Ho= 0.33 - 0.38) as assessed by RAPD. Wright's F statistics showed an Fis value higher than Fst (Fis = 0.164 > Fst = 0.048). AMOVA indicated that 95.12% of the genetic variability is intrapopulational. Even so, both of the genetic markers evaluated showed a relatively high gene flow ((Nm= 15.15), and possibly are still random couplings, although the Fis value was not low. The genetic distance between populations was similarly low for both markers: RAPD (0.012 - 0.016) and Isozymes (0.003 - 0.016). These results show that as assessed by both markers, the populations are genetically similar, and that isozymes (codominant) are the most efficient to detect the population genetic structure. Although isozymes revealed less genetic diversity than RAPDs, the estimated levels of genetic distance were identical. © dos Santos et al. |
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Santos, Joselita Maria Mendes dosFraga, Elmary da CostaMaia, Juracy de FreitasTadei, Wanderli Pedro2020-05-25T20:59:13Z2020-05-25T20:59:13Z2011https://repositorio.inpa.gov.br/handle/1/1617410.2174/1874315301104010011Four populations of Aedes aegypti from Manaus were studied, using allozymes and RAPD loci, to determine intra- and interpopulation genetic variability and differentiation and to compare genetic structure parameters assessed with both markers. Five RAPD primers produced 52 polymorphic fragments, whereas only seven of 18 isozyme loci were polymorphic. The population from Praça 14 was the most polymorphic (P= 94.23% and P= 55.6%); while those from Coroado (P= 82.69% and P= 44.40%) and from Cidade Nova (P= 84.61% and P= 44.40%) were the least polymorphic, for both RAPD and isozymes respectively. The observed heterozygosity was higher between populations (Ho= 0.33 - 0.38) as assessed by RAPD. Wright's F statistics showed an Fis value higher than Fst (Fis = 0.164 > Fst = 0.048). AMOVA indicated that 95.12% of the genetic variability is intrapopulational. Even so, both of the genetic markers evaluated showed a relatively high gene flow ((Nm= 15.15), and possibly are still random couplings, although the Fis value was not low. The genetic distance between populations was similarly low for both markers: RAPD (0.012 - 0.016) and Isozymes (0.003 - 0.016). These results show that as assessed by both markers, the populations are genetically similar, and that isozymes (codominant) are the most efficient to detect the population genetic structure. Although isozymes revealed less genetic diversity than RAPDs, the estimated levels of genetic distance were identical. © dos Santos et al.Volume 4, Número 1, Pags. 11-20Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessIsoenzymeAedes AegyptiComparative StudyControlled StudyDengueFemaleGene FlowGene FrequencyGene LocusGenetic DistancePolymorphism, GeneticGenetic VariabilityGenotypeHeterozygoteHomozygoteMaleMarker GeneNonhumanPriority JournalRandom Amplified Polymorphic DnaGenetic diversity in dengue mosquito, Aedes aegypti (Diptera: Culicidae) from Amazon region: Comparative analysis with isozymes and RAPD lociinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleOpen Tropical Medicine Journalengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfartigo-inpa.pdfapplication/pdf598301https://repositorio.inpa.gov.br/bitstream/1/16174/1/artigo-inpa.pdf6f0bf58ddc1e9f75233f7536ace891b0MD511/161742020-05-25 17:19:06.38oai:repositorio:1/16174Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-05-25T21:19:06Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Genetic diversity in dengue mosquito, Aedes aegypti (Diptera: Culicidae) from Amazon region: Comparative analysis with isozymes and RAPD loci |
title |
Genetic diversity in dengue mosquito, Aedes aegypti (Diptera: Culicidae) from Amazon region: Comparative analysis with isozymes and RAPD loci |
spellingShingle |
Genetic diversity in dengue mosquito, Aedes aegypti (Diptera: Culicidae) from Amazon region: Comparative analysis with isozymes and RAPD loci Santos, Joselita Maria Mendes dos Isoenzyme Aedes Aegypti Comparative Study Controlled Study Dengue Female Gene Flow Gene Frequency Gene Locus Genetic Distance Polymorphism, Genetic Genetic Variability Genotype Heterozygote Homozygote Male Marker Gene Nonhuman Priority Journal Random Amplified Polymorphic Dna |
title_short |
Genetic diversity in dengue mosquito, Aedes aegypti (Diptera: Culicidae) from Amazon region: Comparative analysis with isozymes and RAPD loci |
title_full |
Genetic diversity in dengue mosquito, Aedes aegypti (Diptera: Culicidae) from Amazon region: Comparative analysis with isozymes and RAPD loci |
title_fullStr |
Genetic diversity in dengue mosquito, Aedes aegypti (Diptera: Culicidae) from Amazon region: Comparative analysis with isozymes and RAPD loci |
title_full_unstemmed |
Genetic diversity in dengue mosquito, Aedes aegypti (Diptera: Culicidae) from Amazon region: Comparative analysis with isozymes and RAPD loci |
title_sort |
Genetic diversity in dengue mosquito, Aedes aegypti (Diptera: Culicidae) from Amazon region: Comparative analysis with isozymes and RAPD loci |
author |
Santos, Joselita Maria Mendes dos |
author_facet |
Santos, Joselita Maria Mendes dos Fraga, Elmary da Costa Maia, Juracy de Freitas Tadei, Wanderli Pedro |
author_role |
author |
author2 |
Fraga, Elmary da Costa Maia, Juracy de Freitas Tadei, Wanderli Pedro |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Santos, Joselita Maria Mendes dos Fraga, Elmary da Costa Maia, Juracy de Freitas Tadei, Wanderli Pedro |
dc.subject.eng.fl_str_mv |
Isoenzyme Aedes Aegypti Comparative Study Controlled Study Dengue Female Gene Flow Gene Frequency Gene Locus Genetic Distance Polymorphism, Genetic Genetic Variability Genotype Heterozygote Homozygote Male Marker Gene Nonhuman Priority Journal Random Amplified Polymorphic Dna |
topic |
Isoenzyme Aedes Aegypti Comparative Study Controlled Study Dengue Female Gene Flow Gene Frequency Gene Locus Genetic Distance Polymorphism, Genetic Genetic Variability Genotype Heterozygote Homozygote Male Marker Gene Nonhuman Priority Journal Random Amplified Polymorphic Dna |
description |
Four populations of Aedes aegypti from Manaus were studied, using allozymes and RAPD loci, to determine intra- and interpopulation genetic variability and differentiation and to compare genetic structure parameters assessed with both markers. Five RAPD primers produced 52 polymorphic fragments, whereas only seven of 18 isozyme loci were polymorphic. The population from Praça 14 was the most polymorphic (P= 94.23% and P= 55.6%); while those from Coroado (P= 82.69% and P= 44.40%) and from Cidade Nova (P= 84.61% and P= 44.40%) were the least polymorphic, for both RAPD and isozymes respectively. The observed heterozygosity was higher between populations (Ho= 0.33 - 0.38) as assessed by RAPD. Wright's F statistics showed an Fis value higher than Fst (Fis = 0.164 > Fst = 0.048). AMOVA indicated that 95.12% of the genetic variability is intrapopulational. Even so, both of the genetic markers evaluated showed a relatively high gene flow ((Nm= 15.15), and possibly are still random couplings, although the Fis value was not low. The genetic distance between populations was similarly low for both markers: RAPD (0.012 - 0.016) and Isozymes (0.003 - 0.016). These results show that as assessed by both markers, the populations are genetically similar, and that isozymes (codominant) are the most efficient to detect the population genetic structure. Although isozymes revealed less genetic diversity than RAPDs, the estimated levels of genetic distance were identical. © dos Santos et al. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2020-05-25T20:59:13Z |
dc.date.available.fl_str_mv |
2020-05-25T20:59:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/16174 |
dc.identifier.doi.none.fl_str_mv |
10.2174/1874315301104010011 |
url |
https://repositorio.inpa.gov.br/handle/1/16174 |
identifier_str_mv |
10.2174/1874315301104010011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 4, Número 1, Pags. 11-20 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Open Tropical Medicine Journal |
publisher.none.fl_str_mv |
Open Tropical Medicine Journal |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional do INPA instname:Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
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Instituto Nacional de Pesquisas da Amazônia (INPA) |
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INPA |
institution |
INPA |
reponame_str |
Repositório Institucional do INPA |
collection |
Repositório Institucional do INPA |
bitstream.url.fl_str_mv |
https://repositorio.inpa.gov.br/bitstream/1/16174/1/artigo-inpa.pdf |
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