Guarana (Paullinia cupana) Extract Protects Caenorhabditis elegans Models for Alzheimer Disease and Huntington Disease through Activation of Antioxidant and Protein Degradation Pathways
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/15675 |
Resumo: | Guarana (Paullinia cupana) is largely consumed in Brazil in high energy drinks and dietary supplements because of its stimulant activity on the central nervous system. Although previous studies have indicated that guarana has some protective effects in Parkinson's (PD), Alzheimer's (AD), and Huntington's (HD) disease models, the underlying mechanisms are unknown. Here, we investigated the protective effects of guarana hydroalcoholic extract (GHE) in Caenorhabditis elegans models of HD and AD. GHE reduced polyglutamine (polyQ) protein aggregation in the muscle and also reduced polyQ-mediated neuronal death in ASH sensory neurons and delayed β-amyloid-induced paralysis in a caffeine-independent manner. Moreover, GHE's protective effects were not mediated by caloric restriction, antimicrobial effects, or development and reproduction impairment. Inactivation of the transcription factors SKN-1 and DAF-16 by RNAi partially blocked the protective effects of GHE treatment in the AD model. We show that the protective effect of GHE is associated with antioxidant activity and modulation of proteostasis, since it increased the lifespan and proteasome activity, reduced intracellular ROS and the accumulation of autophagosomes, and increased the expression of SOD-3 and HSP-16.2. Our findings suggest that GHE has therapeutic potential in combating age-related diseases associated with protein misfolding and accumulation. © 2018 Patrícia Ferreira Boasquívis et al. |
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Boasquívis, Patrícia FerreiraSilva, Giovanna Melo MartinsPaiva, Franciny AparecidaCavalcanti, Rodrigo MarinhoNunez, C. V.Paula Oliveira, Riva de2020-05-15T20:46:13Z2020-05-15T20:46:13Z2018https://repositorio.inpa.gov.br/handle/1/1567510.1155/2018/9241308Guarana (Paullinia cupana) is largely consumed in Brazil in high energy drinks and dietary supplements because of its stimulant activity on the central nervous system. Although previous studies have indicated that guarana has some protective effects in Parkinson's (PD), Alzheimer's (AD), and Huntington's (HD) disease models, the underlying mechanisms are unknown. Here, we investigated the protective effects of guarana hydroalcoholic extract (GHE) in Caenorhabditis elegans models of HD and AD. GHE reduced polyglutamine (polyQ) protein aggregation in the muscle and also reduced polyQ-mediated neuronal death in ASH sensory neurons and delayed β-amyloid-induced paralysis in a caffeine-independent manner. Moreover, GHE's protective effects were not mediated by caloric restriction, antimicrobial effects, or development and reproduction impairment. Inactivation of the transcription factors SKN-1 and DAF-16 by RNAi partially blocked the protective effects of GHE treatment in the AD model. We show that the protective effect of GHE is associated with antioxidant activity and modulation of proteostasis, since it increased the lifespan and proteasome activity, reduced intracellular ROS and the accumulation of autophagosomes, and increased the expression of SOD-3 and HSP-16.2. Our findings suggest that GHE has therapeutic potential in combating age-related diseases associated with protein misfolding and accumulation. © 2018 Patrícia Ferreira Boasquívis et al.Volume 2018Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessAmyloid Beta ProteinCaffeineCatechinEpicatechinExtracellular Superoxide DismutaseGlutathione Transferase 4Guarana ExtractMethylxanthinePolyglutamineProteasomeProteinTheobromineTranscription FactorTranscription Factor Daf 16Transcription Factors Skn 1Unclassified DrugAntioxidantPlant ExtractAlzheimer DiseaseAnimals ExperimentAnimals ModelAntioxidant ActivityAutophagosomeBacterial GrowthCaenorhabditis ElegansControlled StudyDpph Radical Scavenging AssayHeat ToleranceHuntington ChoreaLifespanNonhumanProtein AggregationProtein DegradationProtein HomeostasisProtein MisfoldingQuantitative AnalysisRna InterferenceSensory Nerve CellStressAlzheimer DiseaseAnimalsDrug EffectHuntington ChoreaMetabolismPaulliniaAlzheimer DiseaseAnimalAntioxidantsCaenorhabditis ElegansHuntington DiseasePaulliniaPlant ExtractsGuarana (Paullinia cupana) Extract Protects Caenorhabditis elegans Models for Alzheimer Disease and Huntington Disease through Activation of Antioxidant and Protein Degradation Pathwaysinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleOxidative Medicine and Cellular Longevityengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfartigo-inpa.pdfapplication/pdf2378766https://repositorio.inpa.gov.br/bitstream/1/15675/1/artigo-inpa.pdf26a6725dea177968579b6e8489638d37MD511/156752020-05-15 17:44:28.1oai:repositorio:1/15675Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-05-15T21:44:28Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Guarana (Paullinia cupana) Extract Protects Caenorhabditis elegans Models for Alzheimer Disease and Huntington Disease through Activation of Antioxidant and Protein Degradation Pathways |
title |
Guarana (Paullinia cupana) Extract Protects Caenorhabditis elegans Models for Alzheimer Disease and Huntington Disease through Activation of Antioxidant and Protein Degradation Pathways |
spellingShingle |
Guarana (Paullinia cupana) Extract Protects Caenorhabditis elegans Models for Alzheimer Disease and Huntington Disease through Activation of Antioxidant and Protein Degradation Pathways Boasquívis, Patrícia Ferreira Amyloid Beta Protein Caffeine Catechin Epicatechin Extracellular Superoxide Dismutase Glutathione Transferase 4 Guarana Extract Methylxanthine Polyglutamine Proteasome Protein Theobromine Transcription Factor Transcription Factor Daf 16 Transcription Factors Skn 1 Unclassified Drug Antioxidant Plant Extract Alzheimer Disease Animals Experiment Animals Model Antioxidant Activity Autophagosome Bacterial Growth Caenorhabditis Elegans Controlled Study Dpph Radical Scavenging Assay Heat Tolerance Huntington Chorea Lifespan Nonhuman Protein Aggregation Protein Degradation Protein Homeostasis Protein Misfolding Quantitative Analysis Rna Interference Sensory Nerve Cell Stress Alzheimer Disease Animals Drug Effect Huntington Chorea Metabolism Paullinia Alzheimer Disease Animal Antioxidants Caenorhabditis Elegans Huntington Disease Paullinia Plant Extracts |
title_short |
Guarana (Paullinia cupana) Extract Protects Caenorhabditis elegans Models for Alzheimer Disease and Huntington Disease through Activation of Antioxidant and Protein Degradation Pathways |
title_full |
Guarana (Paullinia cupana) Extract Protects Caenorhabditis elegans Models for Alzheimer Disease and Huntington Disease through Activation of Antioxidant and Protein Degradation Pathways |
title_fullStr |
Guarana (Paullinia cupana) Extract Protects Caenorhabditis elegans Models for Alzheimer Disease and Huntington Disease through Activation of Antioxidant and Protein Degradation Pathways |
title_full_unstemmed |
Guarana (Paullinia cupana) Extract Protects Caenorhabditis elegans Models for Alzheimer Disease and Huntington Disease through Activation of Antioxidant and Protein Degradation Pathways |
title_sort |
Guarana (Paullinia cupana) Extract Protects Caenorhabditis elegans Models for Alzheimer Disease and Huntington Disease through Activation of Antioxidant and Protein Degradation Pathways |
author |
Boasquívis, Patrícia Ferreira |
author_facet |
Boasquívis, Patrícia Ferreira Silva, Giovanna Melo Martins Paiva, Franciny Aparecida Cavalcanti, Rodrigo Marinho Nunez, C. V. Paula Oliveira, Riva de |
author_role |
author |
author2 |
Silva, Giovanna Melo Martins Paiva, Franciny Aparecida Cavalcanti, Rodrigo Marinho Nunez, C. V. Paula Oliveira, Riva de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Boasquívis, Patrícia Ferreira Silva, Giovanna Melo Martins Paiva, Franciny Aparecida Cavalcanti, Rodrigo Marinho Nunez, C. V. Paula Oliveira, Riva de |
dc.subject.eng.fl_str_mv |
Amyloid Beta Protein Caffeine Catechin Epicatechin Extracellular Superoxide Dismutase Glutathione Transferase 4 Guarana Extract Methylxanthine Polyglutamine Proteasome Protein Theobromine Transcription Factor Transcription Factor Daf 16 Transcription Factors Skn 1 Unclassified Drug Antioxidant Plant Extract Alzheimer Disease Animals Experiment Animals Model Antioxidant Activity Autophagosome Bacterial Growth Caenorhabditis Elegans Controlled Study Dpph Radical Scavenging Assay Heat Tolerance Huntington Chorea Lifespan Nonhuman Protein Aggregation Protein Degradation Protein Homeostasis Protein Misfolding Quantitative Analysis Rna Interference Sensory Nerve Cell Stress Alzheimer Disease Animals Drug Effect Huntington Chorea Metabolism Paullinia Alzheimer Disease Animal Antioxidants Caenorhabditis Elegans Huntington Disease Paullinia Plant Extracts |
topic |
Amyloid Beta Protein Caffeine Catechin Epicatechin Extracellular Superoxide Dismutase Glutathione Transferase 4 Guarana Extract Methylxanthine Polyglutamine Proteasome Protein Theobromine Transcription Factor Transcription Factor Daf 16 Transcription Factors Skn 1 Unclassified Drug Antioxidant Plant Extract Alzheimer Disease Animals Experiment Animals Model Antioxidant Activity Autophagosome Bacterial Growth Caenorhabditis Elegans Controlled Study Dpph Radical Scavenging Assay Heat Tolerance Huntington Chorea Lifespan Nonhuman Protein Aggregation Protein Degradation Protein Homeostasis Protein Misfolding Quantitative Analysis Rna Interference Sensory Nerve Cell Stress Alzheimer Disease Animals Drug Effect Huntington Chorea Metabolism Paullinia Alzheimer Disease Animal Antioxidants Caenorhabditis Elegans Huntington Disease Paullinia Plant Extracts |
description |
Guarana (Paullinia cupana) is largely consumed in Brazil in high energy drinks and dietary supplements because of its stimulant activity on the central nervous system. Although previous studies have indicated that guarana has some protective effects in Parkinson's (PD), Alzheimer's (AD), and Huntington's (HD) disease models, the underlying mechanisms are unknown. Here, we investigated the protective effects of guarana hydroalcoholic extract (GHE) in Caenorhabditis elegans models of HD and AD. GHE reduced polyglutamine (polyQ) protein aggregation in the muscle and also reduced polyQ-mediated neuronal death in ASH sensory neurons and delayed β-amyloid-induced paralysis in a caffeine-independent manner. Moreover, GHE's protective effects were not mediated by caloric restriction, antimicrobial effects, or development and reproduction impairment. Inactivation of the transcription factors SKN-1 and DAF-16 by RNAi partially blocked the protective effects of GHE treatment in the AD model. We show that the protective effect of GHE is associated with antioxidant activity and modulation of proteostasis, since it increased the lifespan and proteasome activity, reduced intracellular ROS and the accumulation of autophagosomes, and increased the expression of SOD-3 and HSP-16.2. Our findings suggest that GHE has therapeutic potential in combating age-related diseases associated with protein misfolding and accumulation. © 2018 Patrícia Ferreira Boasquívis et al. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2020-05-15T20:46:13Z |
dc.date.available.fl_str_mv |
2020-05-15T20:46:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/15675 |
dc.identifier.doi.none.fl_str_mv |
10.1155/2018/9241308 |
url |
https://repositorio.inpa.gov.br/handle/1/15675 |
identifier_str_mv |
10.1155/2018/9241308 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 2018 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Oxidative Medicine and Cellular Longevity |
publisher.none.fl_str_mv |
Oxidative Medicine and Cellular Longevity |
dc.source.none.fl_str_mv |
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INPA |
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INPA |
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Repositório Institucional do INPA |
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Repositório Institucional do INPA |
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https://repositorio.inpa.gov.br/bitstream/1/15675/1/artigo-inpa.pdf |
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26a6725dea177968579b6e8489638d37 |
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Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA) |
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