Multidrug resistant Pseudomonas aeruginosa survey in a stream receiving effluents from ineffective wastewater hospital plants

• Autor(a) principal: Magalhães, Mary Joyce Targino Lopes
• Data de Publicação: 2016
• Outros Autores: Pontes, Gemilson Soares, Serra, Paula Takita, Balieiro, Antônio Alcirley da Silva, Castro, Diogo Pereira de, Pieri, Fabio Alessandro, Crainey, James Lee, Nogueira, Paulo Afonso, Orlandi, Patrícia Puccinelli
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional do INPA
• Texto Completo: https://repositorio.inpa.gov.br/handle/1/15802
• topic: Amikacin; Aminoglycoside Antibiotic Agent; Ampicillin; Aztreonam; Beta Lactam Antibiotic; Carbapenem Derivative; Cefepime; Ceftazidime; Ceftriaxone; Cephalosporin; Ciprofloxacin; Genomic Dna; Gentamicin; Imipenem; Levofloxacin; Meropenem; Norfloxacin; Piperacillin Plus Tazobactam; Polymyxin B; Restriction Endonuclease; Amikacin; Ampicillin; Antiinfective Agent; Rna 16s; Waste Water; Bacterium Culture; Bacterium Isolate; Bacterium Isolation; Biofilm; Brasil; Effluent; Hospital; Microbial Diversity; Multidrug Resistance; Nonhuman; Polymerase Chain Reaction; Pseudomonas Aeruginosa; Pulsed Field Gel Electrophoresis; Sequence Analysis; Stream (river); Waste Water; Waste Water Treatment Plant; Biodiversity; Dna Fingerprinting; Drug Effects; Genetics; Isolation And Purification; Microbial Sensitivity Test; Microbiology; Physiology; Pseudomonas Aeruginosa; River; Amikacin; Ampicillin; Anti-bacterial Agents; Biodiversity; Biofilms; Brasil; Dna Fingerprinting; Drug Resistance, Multiple, Bacterial; Electrophoresis, Gel, Pulsed-field; Hospitals; Microbial Sensitivity Tests; Pseudomonas Aeruginosa; Rivers; Rna, Ribosomal, 16s; Waste Water

Background: Multi-drug resistant forms of Pseudomonas aeruginosa (MDRPA) are a major source of nosocomial infections and when discharged into streams and rivers from hospital wastewater treatment plants (HWWTP) they are known to be able to persist for extended periods. In the city of Manaus (Western Brazilian Amazon), the effluent of three HWWTPs feed into the urban Mindu stream which crosses the city from its rainforest source before draining into the Rio Negro. The stream is routinely used by Manaus residents for bathing and cleaning (of clothes as well as domestic utensils) and, during periods of flooding, can contaminate wells used for drinking water. Results: 16S rRNA metagenomic sequence analysis of 293 cloned PCR fragments, detected an abundance of Pseudomonas aeruginosa (P. aeruginosa) at the stream's Rio Negro drainage site, but failed to detect it at the stream's source. An array of antimicrobial resistance profiles and resistance to all 14 tested antimicrobials was detected among P. aeruginosa cultures prepared from wastewater samples taken from water entering and being discharged from a Manaus HWWTP. Just one P. aeruginosa antimicrobial resistance profile, however, was detected from cultures made from Mindu stream isolates. Comparisons made between P. aeruginosa isolates' genomic DNA restriction enzyme digest fingerprints, failed to determine if any of the P. aeruginosa found in the Mindu stream were of HWWTP origin, but suggested that Mindu stream P. aeruginosa are from diverse origins. Culturing experiments also showed that P. aeruginosa biofilm formation and the extent of biofilm formation produced were both significantly higher in multi drug resistant forms of P. aeruginosa. Conclusions: Our results show that a diverse range of MDRPA are being discharged in an urban stream from a HWWTP in Manaus and that P. aeruginosa strains with ampicillin and amikacin can persist well within it. © 2016 The Author(s).