Differential expression of genes related to the immune response of anopheles (Nyssorhynchus) darlingi in the brazilian amazon basin

Detalhes bibliográficos
Autor(a) principal: Fernandes, L. R.L.
Data de Publicação: 2018
Outros Autores: Rafael, Míriam Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional do INPA
Texto Completo: https://repositorio.inpa.gov.br/handle/1/14980
Resumo: Anopheles (Nyssorhynchus) darlingi is the primary vector of human malaria in South America. Immune responses in mosquito vectors of malaria are mainly regulated by genes of the Toll and IMD pathways through the transcription factors NF-kappa-β, Rel1 and Rel2, which are controlled by the negative regulatory genes Cactus and Caspar. We measured the expression levels of Rel1, Rel2, Caspar and Cactus genes, which are related to the immune system, in adult females of A. darlingi after blood feeding compared to adult females without blood feeding (controls) due to their possible effects on the ability of becoming infected with species of Plasmodium and spreading malaria. Quantitative expression was determined by real-time PCR, using the reference genes GAPDH and β-actin. The expression levels of Rel1, Rel2, Caspar and Cactus varied significantly at 4, 8, 14 and 24 h in mosquitoes that had fed on blood compared to control insects (0 h), with significantly greater expression at 24 h after blood feeding. Relative expression levels among these genes varied at the different post blood feeding times. This information adds to our understanding of the insect immune response system and related questions involved in understanding the biology and control of this mosquito. © FUNPEC-RP.
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spelling Fernandes, L. R.L.Rafael, Míriam Silva2020-05-07T14:00:17Z2020-05-07T14:00:17Z2018https://repositorio.inpa.gov.br/handle/1/1498010.4238/gmr18076Anopheles (Nyssorhynchus) darlingi is the primary vector of human malaria in South America. Immune responses in mosquito vectors of malaria are mainly regulated by genes of the Toll and IMD pathways through the transcription factors NF-kappa-β, Rel1 and Rel2, which are controlled by the negative regulatory genes Cactus and Caspar. We measured the expression levels of Rel1, Rel2, Caspar and Cactus genes, which are related to the immune system, in adult females of A. darlingi after blood feeding compared to adult females without blood feeding (controls) due to their possible effects on the ability of becoming infected with species of Plasmodium and spreading malaria. Quantitative expression was determined by real-time PCR, using the reference genes GAPDH and β-actin. The expression levels of Rel1, Rel2, Caspar and Cactus varied significantly at 4, 8, 14 and 24 h in mosquitoes that had fed on blood compared to control insects (0 h), with significantly greater expression at 24 h after blood feeding. Relative expression levels among these genes varied at the different post blood feeding times. This information adds to our understanding of the insect immune response system and related questions involved in understanding the biology and control of this mosquito. © FUNPEC-RP.Volume 17, Número 4Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessAdultAmazonasAnimals ExperimentAnopheles DarlingiBloodCactus GeneCaspar GeneControlled StudyFeedingFemaleGeneGene ExpressionImmune ResponseNonhumanPlasmodiumRel1 GeneRel2 GeneRiver BasinDifferential expression of genes related to the immune response of anopheles (Nyssorhynchus) darlingi in the brazilian amazon basininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Researchengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfapplication/pdf254287https://repositorio.inpa.gov.br/bitstream/1/14980/1/artigo-inpa.pdf981195085283025960d7ce493bf855d9MD51CC-LICENSElicense_rdfapplication/octet-stream914https://repositorio.inpa.gov.br/bitstream/1/14980/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD521/149802020-07-14 10:30:24.282oai:repositorio:1/14980Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T14:30:24Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.en.fl_str_mv Differential expression of genes related to the immune response of anopheles (Nyssorhynchus) darlingi in the brazilian amazon basin
title Differential expression of genes related to the immune response of anopheles (Nyssorhynchus) darlingi in the brazilian amazon basin
spellingShingle Differential expression of genes related to the immune response of anopheles (Nyssorhynchus) darlingi in the brazilian amazon basin
Fernandes, L. R.L.
Adult
Amazonas
Animals Experiment
Anopheles Darlingi
Blood
Cactus Gene
Caspar Gene
Controlled Study
Feeding
Female
Gene
Gene Expression
Immune Response
Nonhuman
Plasmodium
Rel1 Gene
Rel2 Gene
River Basin
title_short Differential expression of genes related to the immune response of anopheles (Nyssorhynchus) darlingi in the brazilian amazon basin
title_full Differential expression of genes related to the immune response of anopheles (Nyssorhynchus) darlingi in the brazilian amazon basin
title_fullStr Differential expression of genes related to the immune response of anopheles (Nyssorhynchus) darlingi in the brazilian amazon basin
title_full_unstemmed Differential expression of genes related to the immune response of anopheles (Nyssorhynchus) darlingi in the brazilian amazon basin
title_sort Differential expression of genes related to the immune response of anopheles (Nyssorhynchus) darlingi in the brazilian amazon basin
author Fernandes, L. R.L.
author_facet Fernandes, L. R.L.
Rafael, Míriam Silva
author_role author
author2 Rafael, Míriam Silva
author2_role author
dc.contributor.author.fl_str_mv Fernandes, L. R.L.
Rafael, Míriam Silva
dc.subject.eng.fl_str_mv Adult
Amazonas
Animals Experiment
Anopheles Darlingi
Blood
Cactus Gene
Caspar Gene
Controlled Study
Feeding
Female
Gene
Gene Expression
Immune Response
Nonhuman
Plasmodium
Rel1 Gene
Rel2 Gene
River Basin
topic Adult
Amazonas
Animals Experiment
Anopheles Darlingi
Blood
Cactus Gene
Caspar Gene
Controlled Study
Feeding
Female
Gene
Gene Expression
Immune Response
Nonhuman
Plasmodium
Rel1 Gene
Rel2 Gene
River Basin
description Anopheles (Nyssorhynchus) darlingi is the primary vector of human malaria in South America. Immune responses in mosquito vectors of malaria are mainly regulated by genes of the Toll and IMD pathways through the transcription factors NF-kappa-β, Rel1 and Rel2, which are controlled by the negative regulatory genes Cactus and Caspar. We measured the expression levels of Rel1, Rel2, Caspar and Cactus genes, which are related to the immune system, in adult females of A. darlingi after blood feeding compared to adult females without blood feeding (controls) due to their possible effects on the ability of becoming infected with species of Plasmodium and spreading malaria. Quantitative expression was determined by real-time PCR, using the reference genes GAPDH and β-actin. The expression levels of Rel1, Rel2, Caspar and Cactus varied significantly at 4, 8, 14 and 24 h in mosquitoes that had fed on blood compared to control insects (0 h), with significantly greater expression at 24 h after blood feeding. Relative expression levels among these genes varied at the different post blood feeding times. This information adds to our understanding of the insect immune response system and related questions involved in understanding the biology and control of this mosquito. © FUNPEC-RP.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2020-05-07T14:00:17Z
dc.date.available.fl_str_mv 2020-05-07T14:00:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.inpa.gov.br/handle/1/14980
dc.identifier.doi.none.fl_str_mv 10.4238/gmr18076
url https://repositorio.inpa.gov.br/handle/1/14980
identifier_str_mv 10.4238/gmr18076
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Volume 17, Número 4
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Genetics and Molecular Research
publisher.none.fl_str_mv Genetics and Molecular Research
dc.source.none.fl_str_mv reponame:Repositório Institucional do INPA
instname:Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron:INPA
instname_str Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron_str INPA
institution INPA
reponame_str Repositório Institucional do INPA
collection Repositório Institucional do INPA
bitstream.url.fl_str_mv https://repositorio.inpa.gov.br/bitstream/1/14980/1/artigo-inpa.pdf
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